Performance evaluation of the inverse dynamics method for optimal spacecraft reorientation

The article of record may be found at http://dx.doi.org/10.1016/j.actaastro.2014.11.041This paper investigates the application of the inverse dynamics in the virtual domain method to Euler angles, quaternions, and modified Rodrigues parameters for rapid optimal attitude trajectory generation for spacecraft reorientation maneuvers. The impact of the virtual domain and attitude representation is numerically investigated for both minimum time and minimum energy problems. Owing to the nature of the inverse dynamics method, it yields sub-optimal solutions for minimum time problems. Further- more, the virtual domain improves the optimality of the solution, but at the cost of more computational time. The attitude representation also affects solution quality and compu- tational speed. For minimum energy problems, the optimal solution can be obtained without the virtual domain with any considered attitude representation

Extension of analytical indicial aerodynamics to generic trapezoidal wings in subsonic flow

Analytical indicial aerodynamic functions are calculated for several trapezoidal wings in subsonic flow, with a Mach number 0.3≤ Ma≤ 0.7. The formulation herein proposed extends well-known aerodynamic theories, which are limited to thin aerofoils in incompressible flow, to generic trapezoidal wing planforms. Firstly, a thorough study is executed to assess the accuracy and limitation of analytical predictions, using unsteady results from two state-of-the-art computational fluid dynamics solvers as cross-validated benchmarks. Indicial functions are calculated for a step change in the angle of attack and for a sharp-edge gust, each for four wing configurations and three Mach numbers. Then, analytical and computational indicial responses are used to predict dynamic derivatives and the maximum lift coefficient following an encounter with a one-minus-cosine gust. It is found that the analytical results are in excellent agreement with the computational results for all test cases. In particular, the deviation of the analytical results from the computational results is within the scatter or uncertainty in the data arising from using two computational fluid dynamics solvers. This indicates the usefulness of the developed analytical theories

Applicazione del laser scanner terrestre per la valutazione della condizione delle chiome in Quercus suber L.

The objective of this paper is to show the first results on the evaluation of the possible use of this technique for the estimation of crown condition in cork oak stands (Quercus suber L.). The results show that the TLS (Terrestrial laser Scanner) technology has good potential applications for the deciduous woodlands. The next objective will be to evaluate the performance of this approach in the estimation of the damage caused by insects

Functional Dissection of the PE Domain Responsible for Translocation of PE_PGRS33 across the Mycobacterial Cell Wall

PE are peculiar exported mycobacterial proteins over-represented in pathogenic mycobacterial species. They are characterized by an N-terminal domain of about 110 amino acids (PE domain) which has been demonstrated to be responsible for their export and localization. In this paper, we characterize the PE domain of PE_PGRS33 (PERv1818c), one of the best characterized PE proteins. We constructed several mutated proteins in which portions of the PE domain were deleted or subjected to defined mutations. These proteins were expressed in different mycobacterial species and their localization was characterized. We confirmed that the PE domain is essential for PE_PGRS33 surface localization, and demonstrated that a PE domain lacking its first 30 amino acids loses its function. However, single amino acid substitutions in two regions extremely well conserved within the N-terminal domain of all PE proteins had some effect on the stability of PE_PGRS33, but not on its localization. Using Mycobacterium marinum we could show that the type VII secretion system ESX-5 is essential for PE_PGRS33 export. Moreover, in M. marinum, but not in Mycobacterium bovis BCG and in Mycobacterium tuberculosis, the PE domain of PE_PGRS33 is processed and secreted into the culture medium when expressed in the absence of the PGRS domain. Finally, using chimeric proteins in which different portions of the PERv1818c domain were fused to the N-terminus of the green fluorescent protein, we could hypothesize that the first 30 amino acids of the PE domain contain a sequence that allows protein translocation

Probing the Binding Site of Abl Tyrosine Kinase Using in Situ Click Chemistry

Modern combinatorial chemistry is used to discover compounds with desired function by an alternative strategy, in which the biological target is directly involved in the choice of ligands assembled from a pool of smaller fragments. Herein, we present the first experimental result where the use of in situ click chemistry has been successfully applied to probe the ligand-binding site of Abl and the ability of this enzyme to form its inhibitor. Docking studies show that Abl is able to allow the in situ click chemistry between specific azide and alkyne fragments by binding to Abl-active sites. This report allows medicinal chemists to use protein-directed in situ click chemistry for exploring the conformational space of a ligand-binding pocket and the ability of the protein to guide its inhibitor. This approach can be a novel, valuable tool to guide drug design synthesis in the field of tyrosine kinases

The Tracking performance for the IDEA drift chamber

The IDEA detector concept for a future e$^{+}$e$^{-}$ collider adopts an ultra-low mass drift chamber as a central tracking system. The He-based ultra-low mass drift chamber is designed to provide efficient tracking, a high-precision momentum measurement, and excellent particle identification by exploiting the cluster counting technique. This paper describes the expected tracking performance, obtained with full and fast simulation, for track reconstruction on detailed simulated physics events. Moreover, the details of the construction parameters of the drift chamber, including the inspection of new material for the wires, new techniques for soldering the wires, the development of an improved schema for the drift cell, and the choice of a gas mixture, will be described

Application of simulation modeling for wildfire exposure and transmission assessment in Sardinia, Italy

Abstract The development of comprehensive fire management and risk assessment strategies is of prominent concern in Southern Europe, due to the expanding scale of wildfire risk. In this work, we applied simulation modeling to analyze fine-scale (100-m resolution) wildfire exposure and risk transmission in the 24,000 km2 island of Sardinia (Italy). Sardinia contains a variety of ecological, cultural, anthropic and touristic resources that each summer are threatened by wildfires, and represents well the Mediterranean Basin environments and conditions. Wildfire simulations based on the minimum travel time algorithm were used to characterize wildfire exposure and risk transmission in terms of annual burn probability, flame length, structures exposed and type and amount of transmission. We focused on the historical conditions associated with large (>50 ha) and very large (>200 ha) wildfires that occurred in Sardinia in the period 1998–2016, and combined outputs from wildfire simulation modeling with land uses, building footprint locations, weather, and historical ignition data. The outputs were summarized for weather zones, main wind scenarios and land uses. Our study characterized spatial variations in wildfire spread, exposure and risk transmission among and within weather zones and the main winds associated with large events. This work provides a novel quantitative approach to inform wildfire risk management and planning in Mediterranean areas. The proposed methodology can serve as reference for wildfire risk assessment and can be replicated elsewhere. Findings can be used to better understand the spatial dynamics and patterns of wildfire risk and evaluate expected wildfire behavior or transmission potential in Sardinia and neighboring regions

Cluster counting algorithms for particle identification at future colliders

Recognition of electron peaks and primary ionization clusters in real data-driven waveform signals is the main goal of research for the usage of the cluster counting technique in particle identification at future colliders. The state-of-the-art open-source algorithms fail in finding the cluster distribution Poisson behavior even in low-noise conditions. In this work, we present cutting-edge algorithms and their performance to search for electron peaks and identify ionization clusters in experimental data using the latest available computing tools and physics knowledge.Comment: 6 pages, 12 figures, Proceedings of: ACAT202

Novel site-specific PEGylated L-asparaginase

L-asparaginase (ASNase) from Escherichia coli is currently used in some countries in its PEGylated form (ONCASPAR, pegaspargase) to treat acute lymphoblastic leukemia (ALL). PEGylation refers to the covalent attachment of poly(ethylene) glycol to the protein drug and it not only reduces the immune system activation but also decreases degradation by plasmatic proteases. However, pegaspargase is randomly PEGylated and, consequently, with a high degree of polydispersity in its final formulation. In this work we developed a site-specific N-terminus PEGylation protocol for ASNase. The monoPEG-ASNase was purified by anionic followed by size exclusion chromatography to a final purity of 99%. The highest yield of monoPEG-ASNase of 42% was obtained by the protein reaction with methoxy polyethylene glycol-carboxymethyl N-hydroxysuccinimidyl ester (10kDa) in 100 mM PBS at pH 7.5 and PEG:ASNase ratio of 25:1. The monoPEG-ASNase was found to maintain enzymatic stability for more days than ASNase, also was resistant to the plasma proteases like asparaginyl endopeptidase and cathepsin B. Additionally, monoPEG-ASNase was found to be potent against leukemic cell lines (MOLT-4 and REH) in vitro like polyPEG-ASNase. monoPEG-ASNase demonstrates its potential as a novel option for ALL treatment, being an inventive novelty that maintains the benefits of the current enzyme and solves challenges.publishe

GIARPS: the unique VIS-NIR high precision radial velocity facility in this world

GIARPS (GIAno & haRPS) is a project devoted to have on the same focal station of the Telescopio Nazionale Galileo (TNG) both the high resolution spectrographs HARPS-N (VIS) and GIANO (NIR) working simultaneously. This could be considered the first and unique worldwide instrument providing cross-dispersed echelle spectroscopy at a high resolution (R=115,000 in the visual and R=50,000 in the IR) and over in a wide spectral range (0.383 - 2.45 micron) in a single exposure. The science case is very broad, given the versatility of such an instrument and the large wavelength range. A number of outstanding science cases encompassing mainly extra-solar planet science starting from rocky planet search and hot Jupiters, atmosphere characterization can be considered. Furthermore both instrument can measure high precision radial velocity by means the simultaneous thorium technique (HARPS - N) and absorbing cell technique (GIANO) in a single exposure. Other science cases are also possible. Young stars and proto-planetary disks, cool stars and stellar populations, moving minor bodies in the solar system, bursting young stellar objects, cataclysmic variables and X-ray binary transients in our Galaxy, supernovae up to gamma-ray bursts in the very distant and young Universe, can take advantage of the unicity of this facility both in terms of contemporaneous wide wavelength range and high resolution spectroscopy.Comment: 8 pages, 5 figures, SPIE Conference Proceeding