Drought reduces transmission of Turnip yellows virus , an insect-vectored circulative virus

Application of a severe water deficit to Arabidopsis thaliana plants infected with a mutant of Turnip yellows virus (TuYV, Family Luteoviridae) triggers a significant alteration of several plant phenology traits and strongly reduces the transmission efficiency of the virus by aphids. Although virus accumulation in water-stressed plants was similar to that in plants grown under well-watered conditions, virus accumulation was reduced in aphids fed on plant under water deficit. These results suggest alteration of the aphid feeding behavior on plants under water deficit

The Genome Sequence of the Grape Phylloxera Provides Insights into the Evolution, Adaptation, and Invasion Routes of an Iconic Pest

Background: Although native to North America, the invasion of the aphid-like grape phylloxera Daktulosphaira vitifoliae across the globe altered the course of grape cultivation. For the past 150 years, viticulture relied on grafting-resistant North American Vitis species as rootstocks, thereby limiting genetic stocks tolerant to other stressors such as pathogens and climate change. Limited understanding of the insect genetics resulted in successive outbreaks across the globe when rootstocks failed. Here we report the 294-Mb genome of D. vitifoliae as a basic tool to understand host plant manipulation, nutritional endosymbiosis, and enhance global viticulture. Results: Using a combination of genome, RNA, and population resequencing, we found grape phylloxera showed high duplication rates since its common ancestor with aphids, but similarity in most metabolic genes, despite lacking obligate nutritional symbioses and feeding from parenchyma. Similarly, no enrichment occurred in development genes in relation to viviparity. However, phylloxera evolved > 2700 unique genes that resemble putative effectors and are active during feeding. Population sequencing revealed the global invasion began from the upper Mississippi River in North America, spread to Europe and from there to the rest of the world. Conclusions: The grape phylloxera genome reveals genetic architecture relative to the evolution of nutritional endosymbiosis, viviparity, and herbivory. The extraordinary expansion in effector genes also suggests novel adaptations to plant feeding and how insects induce complex plant phenotypes, for instance galls. Finally, our understanding of the origin of this invasive species and its genome provide genetics resources to alleviate rootstock bottlenecks restricting the advancement of viticulture

Impact of Abiotic Stresses on Plant Virus Transmission by Aphids

Plants regularly encounter abiotic constraints, and plant response to stress has been a focus of research for decades. Given increasing global temperatures and elevated atmospheric CO 2 levels and the occurrence of water stress episodes driven by climate change, plant biochemistry, in particular, plant defence responses, may be altered significantly. Environmental factors also have a wider impact, shaping viral transmission processes that rely on a complex set of interactions between, at least, the pathogen, the vector, and the host plant. This review considers how abiotic stresses influence the transmission and spread of plant viruses by aphid vectors, mainly through changes in host physiology status, and summarizes the latest findings in this research field. The direct effects of climate change and severe weather events that impact the feeding behaviour of insect vectors as well as the major traits (e.g., within-host accumulation, disease severity and transmission) of viral plant pathogens are discussed. Finally, the intrinsic capacity of viruses to react to environmental cues in planta and how this may influence viral transmission efficiency is summarized. The clear interaction between biotic (virus) and abiotic stresses is a risk that must be accounted for when modelling virus epidemiology under scenarios of climate change

Drought alters epidemiological traits of phytoviruses

National audienc

Water deficit enhances the transmission of plant viruses by insect vectors_raw data.xlsx

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Plant-virus-vector interactions under water deficit. Relationships between epidemiological traits of the Cauliflower mosaic virus and Arabidopsis thaliana growth-related traits under artificial drought

National audienc

Effet d'un stress hydrique sur l'épidémiologie d'un virus non circulant, le Cauliflower mosaic virus et adaptation des plantes à la sècheresse

National audienceLa production agricole et le fonctionnement des écosystèmes sontfréquemment contraints par des épisodes de sécheresse dont la fréquence e tla durée devraient augmenter sous l'influence des changements climatiques. Les recherches en agronomie doivent permettre de proposer une évolution des pratiques agricoles et de gestion durable des écosystèmes dans un contexte de limitation des ressources en eau. Les interactions entre les plantes, leur scortèges de pathogènes, de symbiotes, de vecteurs et de l'environnement abiotique sont encore trop peu intégrés à ces recherches. A ce jour, 900 phytovirus ont été décrits, infectant environ 70% des plantes et générant des pertes majeures de productivité. Outre l'impact négatif des virus sur leurs plantes hôtes, un aspect inattendu mais convergent de ces virus, émerge d'après la littérature et des résultats préliminaires effectués à BGPI (par Manuella VanMunster). Dans certains cas, une infection virale pourrait être bénéfique, en améliorant la tolérance des plantes à des stress abiotiques comme un déficiten eau. Dans d'autres cas, la transmission virale serait plus importante en contexte de sécheresse. Ces résultats pourraient être en lien avec la granderéactivité des phytovirus à percevoir et réagir (en termes d'efficacité de transmission et de virulence) en réponse à un déficit hydrique du sol. Les buts du projet sont d'évaluer les paramètres épidémiologiques (efficacitéde transmission, charge virale, virulence) et éco physiologiques de plantes infectées en conditions de stress hydrique sévère et d'analyser les effets délétères ou bénéfiques du CaMV dans ces conditions. Deux questions seront abordées : l'infection virale améliore-t-elle la tolérance des plantes à un stress hydrique sévère ? Quelles sont les conséquences d'un déficit hydrique sévère sur l'épidémiologie virale

From stylet to cuticle: Transcriptomics and genomics of aphid cuticular protein

International audienc

Can plants use an entomopathogenic virus as a defense against herbivores?

International audienceIt is by now well established that plants use various strategies to defend themselves against herbivores. Besides conventional weapons such as spines and stinging hairs and sophisticated chemical defenses, plants can also involve the enemies of the herbivores in their defense. It has been suggested that plants could even use entomopathogens as part of their defense strategies. In this paper, we show that Brassica oleraceae plants that are attacked by Myzus persicae aphids infected with an entomopathogenic parvovirus (M. persicae densovirus) transport the virus through the phloem locally and systematically. Moreover, healthy aphids that fed on the same leaf, but separated from infected aphids were infected via the plant. Hence, this is proof of the principle that plants can be vectors of an insect virus and can possibly use this virus as a defense against herbivores