Characterization of semigroup by rough interval pythagorean fuzzy set

This paper expose a study on rough interval valued pythagorean fuzzy sets in semigroups. We characterize rough interval valued pythagorean fuzzy sets by an example. Characterize composition of two interval valued pythagorean fuzzy sets. Introduce rough interval valued pythagorean fuzzy left(right, bi-, interior-,(1,2)-)ideals in semigroups. Moreover we prove an interval valued pythagorean fuzzy set is an upper rough interval valued pythagorean fuzzy left(right) ideal of semigroup also we give an example for converse of this is not true. Lower and upper approximation of an interval valued pythagorean fuzzy ideal of semigroup is an interval valued pythagorean fuzzy ideal of semigroup.Publisher's Versio

A subset of human limbal epithelial cells with greater nucleus-to-cytoplasm ratio expressing high levels of p63 possesses slow-cycling property

Purpose: The purpose of this study was to evaluate the subset of limbal epithelial cells with greater nucleus-to-cytoplasm (N/C) ratio expressing high levels of p63 for their slow-cycling property, a characteristic feature of stem cells (SCs). Methods: Limbal and peripheral corneal explant cultures were pulse labeled with 5-5-bromo-2'-deoxyuridine (BrdU) for 5 days, followed by a period of 3-week chase. Cultured explants were cryosectioned and stained for BrdU. The epithelial cells in the outgrowth and those remaining on the explant were isolated and subjected to cytospin and double immunostaining for BrdU and p63, followed by identification of label-retaining cells (LRCs) and quantification of p63 expression using confocal microscopy. Results: A distinct population of small cells with large N/C ratio expressing high levels of p63 retained the BrdU label after 21-day chase. Further, this population of LRCs, negative for the differentiation marker K3, was observed in the epithelial outgrowth of limbal but not in that of peripheral cornea. LRCs were seen to migrate along the cut edge of limbal explants in culture and were also observed as clusters of small cells in the outgrowth, which contained cells with the ability to form holoclone colonies. Conclusions: These results demonstrate that the small cells with large N/C ratio and high levels of p63 have BrdU label retaining slo-cycling property, thus confirming that these 2 parameters in combination may serve as a precise marker for identification and quantification of ex vivo-expanded limbal SCs. This method would be useful to standardize the optimal culture conditions that can maintain and expand SCs for therapeutic applications

High expression of p63 combined with a large N/C ratio defines a subset of human limbal epithelial cells: implications on epithelial stem cells

Purpose: To characterize human limbal epithelial cells based on the expression levels of nuclear protein p63 and the nucleus-to-cytoplasm (N/C) ratio. Methods: Limbal, peripheral, and central corneal epithelia were separated from the stroma by Dispase II and subsequently were treated with trypsin to obtain single-cell suspensions. Cytospin smears of the cell suspensions were double immunostained for p63 and then stained for any one of the markers (acidic cytokeratins [AE1], K5, K3, or connexin 43 [C×43]). They were counterstained with propidium iodide. More than 100 cells from each zone were analyzed for p63 expression levels and nuclear/cellular area using quantitative confocal microscopy. Results: A gradient of p63-positive cells was observed in corneal and limbal epithelial cells. The percentage of p63-positive cells and the level of p63 expression were significantly higher in the limbal than in the peripheral or central corneal epithelium. Two-parameter (p63 levels and N/C ratio) analysis revealed the presence of a distinct population of small cells with higher levels of p63 and a large N/C ratio in the limbal epithelium. Such limbal epithelial cells were positive for AE1 and K5 but negative for K3 and C×43. Conclusions: These results suggest that this distinct group of small cells in the limbal epithelium with greater N/C ratio, expressing high levels of nuclear protein p63, probably represent corneal epithelial stem cells

Toward allele-specific targeting therapy and pharmacodynamic marker for spinocerebellar ataxia type 3

Spinocerebellar ataxia type 3 (SCA3), caused by a CAG repeat expansion in the ataxin-3 gene (ATXN3), is characterized by neuronal polyglutamine (polyQ) ATXN3 protein aggregates. Although there is no cure for SCA3, gene-silencing approaches to reduce toxic polyQ ATXN3 showed promise in preclinical models. However, a major limitation in translating putative treatments for this rare disease to the clinic is the lack of pharmacodynamic markers for use in clinical trials. Here, we developed an immunoassay that readily detects polyQ ATXN3 proteins in human biological fluids and discriminates patients with SCA3 from healthy controls and individuals with other ataxias. We show that polyQ ATXN3 serves as a marker of target engagement in human fibroblasts, which may bode well for its use in clinical trials. Last, we identified a single-nucleotide polymorphism that strongly associates with the expanded allele, thus providing an exciting drug target to abrogate detrimental events initiated by mutant ATXN3. Gene-silencing strategies for several repeat diseases are well under way, and our results are expected to improve clinical trial preparedness for SCA3 therapies

First characterization of the SPADnet sensor:a digital silicon photomultiplier for PET applications

Silicon Photomultipliers have the ability to replace photomultiplier tubes when used as light sensors in scintillation gamma-ray detectors. Their timing properties, compactness, and magnetic field compatibility make them interesting for use in Time-of-Flight Magnetic Resonance Imaging compatible Positron Emission Tomography. In this paper, we present a new fully digital Single Photon Avalanche Diode (SPAD) based detector fabricated in CMOS image sensor technology. It contains 16x8 pixels with a pitch of 610x571.2 mu m(2). The Dark Count Rate and the Photon Detection Probability of each SPAD has been measured and the homogeneity of these parameters in the entire 92000 SPAD array is shown. The sensor has been optically coupled to a single LYSO needle and a LYSO array. The scintillator crystal was irradiated with several gamma sources and the resulting images and energy spectra are presented

SPADnet: Embedded coincidence in a smart sensor network for PET applications

n this paper we illustrate the core technologies at the basis of the European SPADnet project (www.spadnet.eu), and present the corresponding first results. SPADnet is aimed at a new generation of MRI-compatible, scalable large area image sensors, based on CMOS technology, that are networked to perform gamma-ray detection and coincidence to be used primarily in (Time-of-Flight) Positron Emission Tomography (PET). The project innovates in several areas of PET systems, from optical coupling to single-photon sensor architectures, from intelligent ring networks to reconstruction algorithms. In addition, SPADnet introduced the first computational model enabling study of the full chain from gamma photons to network coincidence detection through scintillation events, optical coupling, etc

Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

The question whether taxonomic descriptions naming new animal species without type specimen(s) deposited in collections should be accepted for publication by scientific journals and allowed by the Code has already been discussed in Zootaxa (Dubois & Nemésio 2007; Donegan 2008, 2009; Nemésio 2009a–b; Dubois 2009; Gentile & Snell 2009; Minelli 2009; Cianferoni & Bartolozzi 2016; Amorim et al. 2016). This question was again raised in a letter supported by 35 signatories published in the journal Nature (Pape et al. 2016) on 15 September 2016. On 25 September 2016, the following rebuttal (strictly limited to 300 words as per the editorial rules of Nature) was submitted to Nature, which on 18 October 2016 refused to publish it. As we think this problem is a very important one for zoological taxonomy, this text is published here exactly as submitted to Nature, followed by the list of the 493 taxonomists and collection-based researchers who signed it in the short time span from 20 September to 6 October 2016

In vitro Shoot multiplication of Physalis minima L. - an important Medicinal Herb

The present study was to develop an efficient protocol for shoot multiplication of Physalis minima L. Shoots were cultured using nodal segments from 20 days old field grown young plant. The nodal explants were cultured on MS medium supplemented with different concentrations of BAP and KIN. The two cytokinins tested, KIN was found to respond well in shoot multiplication and number of shoots from the nodal explants when compared to BAP. Large number of shoots was induced from all the concentrations of both BAP and KIN. BAP was found to develop in shoot multiplication and higher number of shoots from the nodal explants when compared to KIN. The highest frequency of 100% shoot induction was observed on MS basal medium supplemented with 8μM BAP and 10 μM KIN. Matured shoots are isolated and then transferred to the MS basal medium supplemented with  different concentration of NAA and IBA for root induction. The higher number of roots were produced in 30 days on MS basal medium supplemented with 10 μM IBA and 6 μM NAA. The well rooted plantlets were isolated and transplanted to the paper cup for hardening and the well established plants were transferred to the field for acclimatization

In vitro rapid multiplication of Coldenia procumbens L. from shoot tip explants

An efficient protocol for in vitro rapid multiplication of Coldenia procumbens L. has been developed. The shoot tip explants were cultured on MS basal medium supplemented with different concentrations of BAP and KIN. The two cytokinins are tested, BAP was found to develop higher number of shoots from the shoot tip explants when compared to KIN. higher number of shoots was produced from all the concentrations of BAP and KIN. The highest  frequency of 100% shoot induction was observed on MS basal medium supplemented with 6 μM BAP and 10 μM KIN. The number of shoots observed on the MS basal medium supplemented with BAP  obtained  14 as well as 11 in KIN. The data's are recorded after 30days of inoculation

Effect of Macrotyloma Uniflorum on Free Radicals and An-tioxidants in Tissues of High Fructose-fed Rats

Feeding rats with high fructose, induces insulin resistance, hyperinsulinaemia, elevation of blood glucose level and impaired glucose tolerance. Oxidative stress plays a vital role in pathology associated with insulin resistance. The present study aimed to investigate the effects of Macrotyloma uniflorum (M. uniflorum) on the oxidant-antioxidant status in liver, kidney and heart of high fructose-fed diet (HFFD) rats. Male albino Wistar rats (160-180 g) were divided into six groups. Groups I and II received control diet (Group I served as normal control, group II received M.uniflorum (1000 mg/kg). Groups III-IV received HFFD (groups IV-VI received 250, 500 and 1000 mg/kg of M. uniflorum respectively). The HFFD fed rats showed increased levels of glucose, thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), lipid hydroperoxides (HP) and impaired antioxidant defense as evidenced by decreased in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) glutathione reductase (GR) and the levels of reduced gluatathione (GSH). Treatment with M.uniflorum to the fructose-fed rats mitigated these alterations. M. uniflorum administration for 15 days decreased glucose levels; lipid peroxidation and restored the antioxidant potential. These findings support and strengthen the utility of M. uniflorum in the management of IR and associated pathology during diabetes.  &nbsp