Analyse multi-physique pour la tenue en puissance des dispositifs passifs hyperfréquences : prise en compte des boîtiers métalliques

National audienceAnalyse multi-physique pour la tenue en puissance des dispositifs passifs hyperfréquences : prise en compte des boîtiers métalliques Résumé Ce papier propose une analyse de la capacité en puissance (APHC) de dispositifs passifs en technologie micro-ruban. Cette étude a pour originalité de prendre en compte les conditions environnementales telles que le boîtier métallique, classiquement utilisé dans les systèmes hyperfréquences, la convection, le rayonnement et la température extérieure. Une méthode systématique et analytique est tout d'abord développée pour l'analyse de la capacité en puissance en prenant en considération les transferts de chaleur par convection et par rayonnement. La méthode et le modèle proposés sont validés par simulation multi-physique et mesure de profils thermiques sur deux filtres coupe-bande à 10 GHz

Fast Evaluation of the Fatigue Lifetime of Elastomers Based on a Heat Build-up Protocol and Micro-tomography Measurements

WOSInternational audienceThe temperature of rubber-like materials increases under cyclic loadings, due to their dissipative behaviour and low thermal conductivity. This well-known phenomenon, called heat build-up, has attracted the attention of researchers for a long time. But, to our knowledge, no published studies have tried to link this temperature rise to fatigue behaviour, as already done for many metallic materials. Two main points are discussed in this paper. The first one is dedicated to heat build-up measurements: a specific experimental protocol was developed to capture the instantaneous heat build-up and, based on this protocol, a "heat build-up test" was defined in order to link the temperature rise to the principal maximum strain, which is a commonly used variable for fatigue criterion. A discussion on the correlation between these results and the fatigue behaviour is opened. This relation is illustrated for several industrial materials by a comparison between heat build-up measurements and fatigue life duration. The second point investigates the ability to couple X-ray tomography measurements presented elsewhere [1] to the former heat build-up results in order to predict the initiation lifetime. An approach based on a critical energy criterion was proposed and the comparison to a classic Wöhler curve approach gave very good results

ABCC6 is a basolateral plasma membrane protein

RATIONALE:: ABCC6 plays a crucial role in ectopic calcification; mutations of the gene cause pseudoxanthoma elasticum and general arterial calcification of infancy. To elucidate the role of ABCC6 in cellular physiology and disease, it is crucial to establish the exact subcellular localization of the native ABCC6 protein. OBJECTIVE:: In a recent article in Circulation Research, ABCC6 was reported to localize to the mitochondria-associated membrane and not the plasma membrane. As the suggested mitochondrial localization is inconsistent with published data and the presumed role of ABCC6, we performed experiments to determine the cellular localization of ABCC6 in its physiological environment. METHODS AND RESULTS:: We performed immunofluorescent labeling of frozen mouse and human liver sections, as well as primary hepatocytes. We used several different antibodies recognizing human and mouse ABCC6. Our results unequivocally show that ABCC6 is in the basolateral membrane of hepatocytes and is not associated with the mitochondria, mitochondria-associated membrane, or the endoplasmic reticulum. CONCLUSIONS:: Our findings support the model that ABCC6 is in the basolateral membrane, mediating the sinusoidal efflux of a metabolite from the hepatocytes to systemic circulation. © 2013 American Heart Association, Inc

Alteration of Extracellular Nucleotide Metabolism in Pseudoxanthoma Elasticum

Pseudoxanthoma elasticum (PXE) is a rare genetic condition primarily caused by hepatic ABCC6 transporter dysfunction. Most clinical manifestations of PXE are due to premature calcification of elastic fibers. However, the vascular impact of PXE is pleiotropic and remains ill defined. ABCC6 expression has recently been associated with cellular nucleotide export. We studied the impact of ABCC6 deficiency on blood levels of adenosine triphosphate and related metabolites and on soluble nucleotidase activities in PXE patients and Abcc6 mice. In addition, we investigated the expression of genes encoding ectocellular purinergic signaling proteins in mouse liver and aorta. Plasma adenosine triphosphate and pyrophosphate levels were significantly reduced in PXE patients and in Abcc6 mice, whereas adenosine concentration was not modified. Moreover, 5\u27-nucleotidase/CD73 activity was increased in the serum of PXE patients and Abcc6 mice. Consistent with alterations of purinergic signaling, the expression of genes involved in purine and phosphate transport/metabolism was dramatically modified in Abcc6 mouse aorta, with much less impact on the liver. ABCC6 deficiency causes impaired vascular homeostasis and tissue perfusion. Our findings suggest that these alterations are linked to changes in extracellular nucleotide metabolism that are remote from the liver. This opens new perspectives for the understanding of PXE pathophysiology

Dietary Pyrophosphate Modulates Calcification in a Mouse Model of Pseudoxanthoma Elasticum: Implication for Treatment of Patients

Pseudoxanthoma elasticum (PXE) is a heritable disease caused by ABCC6 deficiency. Patients develop ectopic calcification in skin, eyes and vascular tissues. ABCC6, primarily found in liver and kidneys, mediates the cellular efflux of ATP, which is rapidly converted into pyrophosphate (PPi), a potent inhibitor of calcification. PXE patients and Abcc6 mice display reduced PPi levels in plasma and peripheral tissues. PXE is currently incurable, although some palliative treatments exist. In recent years, we have successfully developed therapeutic methodologies to compensate the PPi deficit in animal models and humans. Here, we inadvertently discovered that modulating dietary PPi can also be an effective approach to reducing calcification in Abcc6 mice. Our findings were prompted by a change in institutional rodent diet. The new chow was enriched in PPi, which increased plasma PPi, and significantly reduced mineralization in Abcc6 mice. We also found that dietary PPi is readily absorbed in humans. Our results suggest that the consumption of food naturally or artificially enriched in PPi represents a possible intervention to mitigate calcification progression in PXE, that dietary preferences of patients may explain PXE heterogeneous manifestations and that animal chow has the potential to influence data reproducibility

Two-dimensional simulations of solar-like models with artificially enhanced luminosity. II. Impact on internal gravity waves

This is the final version. Available from EDP Sciences via the DOI in this recordArtificially increasing the luminosity and the thermal diffusivity of a model is a common tactic adopted in hydrodynamical simulations of stellar convection. In this work, we analyse the impact of these artificial modifications on the physical properties of stellar interiors and specifically on internal gravity waves. We perform two-dimensional simulations of solar-like stars with the MUSIC code. We compare three models with different luminosity enhancement factors to a reference model. The results confirm that properties of the waves are impacted by the artificial enhancement of the luminosity and thermal diffusivity. We find that an increase in the stellar luminosity yields a decrease in the bulk convective turnover timescale and an increase in the characteristic frequency of excitation of the internal waves. We also show that a higher energy input in a model, corresponding to a larger luminosity, results in higher energy in high frequency waves. Across our tests with the luminosity and thermal diffusivity enhanced together by up to a factor of 104, our results are consistent with theoretical predictions of radiative damping. Increasing the luminosity also has an impact on the amplitude of oscillatory motions across the convective boundary. One must use caution when interpreting studies of internal gravity waves based on hydrodynamical simulations with artificially enhanced luminosity.Science and Technology Facilities Council (STFC)European Research Council (ERC

Further investigation of confirmed urinary tract infection (UTI) in children under five years: a systematic review.

Background: Further investigation of confirmed UTI in children aims to prevent renal scarring and future complications. Methods: We conducted a systematic review to determine the most effective approach to the further investigation of confirmed urinary tract infection (UTI) in children under five years of age. Results: 73 studies were included. Many studies had methodological limitations or were poorly reported. Effectiveness of further investigations: One study found that routine imaging did not lead to a reduction in recurrent UTIs or renal scarring. Diagnostic accuracy: The studies do not support the use of less invasive tests such as ultrasound as an alternative to renal scintigraphy, either to rule out infection of the upper urinary tract (LR- = 0.57, 95%CI: 0.47, 0.68) and thus to exclude patients from further investigation or to detect renal scarring (LR+ = 3.5, 95% CI: 2.5, 4.8). None of the tests investigated can accurately predict the development of renal scarring. The available evidence supports the consideration of contrast-enhanced ultrasound techniques for detecting vesico-ureteric reflux (VUR), as an alternative to micturating cystourethrography (MCUG) (LR+ = 14.1, 95% CI: 9.5, 20.8; LR- = 0.20, 95%CI: 0.13, 0.29); these techniques have the advantage of not requiring exposure to ionising radiation. Conclusion: There is no evidence to support the clinical effectiveness of routine investigation of children with confirmed UTI. Primary research on the effectiveness, in terms of improved patient outcome, of testing at all stages in the investigation of confirmed urinary tract infection is urgently required

YSE-PZ: A Transient Survey Management Platform that Empowers the Human-in-the-Loop

The modern study of astrophysical transients has been transformed by an exponentially growing volume of data. Within the last decade, the transient discovery rate has increased by a factor of ~20, with associated survey data, archival data, and metadata also increasing with the number of discoveries. To manage the data at this increased rate, we require new tools. Here we present YSE-PZ, a transient survey management platform that ingests multiple live streams of transient discovery alerts, identifies the host galaxies of those transients, downloads coincident archival data, and retrieves photometry and spectra from ongoing surveys. YSE-PZ also presents a user with a range of tools to make and support timely and informed transient follow-up decisions. Those subsequent observations enhance transient science and can reveal physics only accessible with rapid follow-up observations. Rather than automating out human interaction, YSE-PZ focuses on accelerating and enhancing human decision making, a role we describe as empowering the human-in-the-loop. Finally, YSE-PZ is built to be flexibly used and deployed; YSE-PZ can support multiple, simultaneous, and independent transient collaborations through group-level data permissions, allowing a user to view the data associated with the union of all groups in which they are a member. YSE-PZ can be used as a local instance installed via Docker or deployed as a service hosted in the cloud. We provide YSE-PZ as an open-source tool for the community.Comment: 23 pages, 9 figures, submitted to PAS

Author correction : a global database for metacommunity ecology, integrating species, traits, environment and space

Correction to: Scientific Data https://doi.org/10.1038/s41597-019-0344-7, published online 08 January 202

Author correction : a global database for metacommunity ecology, integrating species, traits, environment and space

Correction to: Scientific Data https://doi.org/10.1038/s41597-019-0344-7, published online 08 January 202