Hepatitis C virus quasispecies in chronically infected children subjected to interferon–ribavirin therapy

Accumulating evidence suggests that certain features of hepatitis C virus (HCV), especially its high genetic variability, might be responsible for the low efficiency of anti-HCV treatment. Here, we present a bioinformatic analysis of HCV-1a populations isolated from 23 children with chronic hepatitis C (CHC) subjected to interferon–ribavirin therapy. The structures of the viral quasispecies were established based on a 132-amino-acid sequence derived from E1/E2 protein, including hypervariable region 1 (HVR1). Two types of HCV populations were identified. The first type, found in non-responders, contained a small number of closely related variants. The second type, characteristic for sustained responders, was composed of a large number of distantly associated equal-rank variants. Comparison of 445 HVR1 sequences showed that a significant number of variants present in non-responding patients are closely related, suggesting that certain, still unidentified properties of the pathogen may be key factors determining the result of CHC treatment

Latitude dictates plant diversity effects on instream decomposition

Running waters contribute substantially to global carbon fluxes through decomposition of terrestrial plant litter by aquatic microorganisms and detritivores. Diversity of this litter may influence instream decomposition globally in ways that are not yet understood. We investigated latitudinal differences in decomposition of litter mixtures of low and high functional diversity in 40 streams on 6 continents and spanning 113 degrees of latitude. Despite important variability in our dataset, we found latitudinal differences in the effect of litter functional diversity on decomposition, which we explained as evolutionary adaptations of litter-consuming detritivores to resource availability. Specifically, a balanced diet effect appears to operate at lower latitudes versus a resource concentration effect at higher latitudes. The latitudinal pattern indicates that loss of plant functional diversity will have different consequences on carbon fluxes across the globe, with greater repercussions likely at low latitudes

Review on Fake Iris Detection Method using Image Quality Assessment

Abstract A biometric system is a computer system which is used to identify the person on their behavioral and physiological characteristic for example fingerprint, face, iris, key-stroke, signature, voice, et

Environmental status of some beaches in Daman and South Gujarat, India

641-649Beaches are the prime choice of amusement, hence determining its quality is significant to public health. The existing status of water and sediment quality of four beaches along the north-west coast of India is evaluated at high and low tide (for three weeks) during the pre-monsoon season. Water samples were measured for hydrography, nutrients, Petroleum Hydrocarbons (PHc) and Fecal Coliform (FC). Sediments were analyzed for heavy metals, PHc and FC. Results indicated higher concentrations of ammonia (28.7 μmol/l) and phosphate (57.3 μmol/l) in the waters of Tadgam beach owing to the proximity to industrial discharge. Metals such as Fe, Al, Mn, Cr, Zn, Cu, Ni, Co, and Hg were found to be above Lowest Effective Range (LER) as compared to Cr and Fe, those exceed Severe Effective Range (SER). Elevated levels of FC in both water and sediment in all the studied beaches indicated microbial contamination relative to anthropogenic activities

NUMA-aware graph-structured analytics

Graph-structured analytics has been widely adopted in a number of big data applications such as social computation, web-search and recommendation systems. Though much prior research focuses on scaling graph-analytics on distributed environments, the strong desire on performance per core, dollar and joule has generated considerable interests of processing large-scale graphs on a single server-class machine, which may have several terabytes of RAM and 80 or more cores. However, prior graph-analytics systems are largely neutral to NUMA characteristics and thus have suboptimal performance. This paper presents a detailed study of NUMA characteristics and their impact on the efficiency of graph-analytics. Our study uncovers two insights: 1) either random or interleaved allocation of graph data will significantly hamper data locality and paral-lelism; 2) sequential inter-node (i.e., remote) memory accesses have much higher bandwidth than both intra- and inter-node ran-dom ones. Based on them, this paper describes Polymer, a NUMA-aware graph-analytics system on multicore with two key design decisions. First, Polymer differentially allocates and places topol-ogy data, application-defined data and mutable runtime states of a graph system according to their access patterns to minimize remote accesses. Second, for some remaining random accesses, Polymer carefully converts random remote accesses into sequential remote accesses, by using lightweight replication of vertices across NUMA nodes. To improve load balance and vertex convergence, Polymer is further built with a hierarchical barrier to boost parallelism and locality, an edge-oriented balanced partitioning for skewed graphs, and adaptive data structures according to the proportion of ac-tive vertices. A detailed evaluation on an 80-core machine shows that Polymer often outperforms the state-of-the-art single-machine graph-analytics systems, including Ligra, X-Stream and Galois, for a set of popular real-world and synthetic graphs

Ribosomal protein SA haploinsufficiency in humans with isolated congenital asplenia

Isolated congenital asplenia (ICA) is characterized by the absence of a spleen at birth in individuals with no other developmental defects. The patients are prone to life-threatening bacterial infections. The unbiased analysis of exomes revealed heterozygous mutations in RPSA in 18 patients from eight kindreds, corresponding to more than half the patients and over one-third of the kindreds studied. The clinical penetrance in these kindreds is complete. Expression studies indicated that the mutations carried by the patients—a nonsense mutation, a frameshift duplication, and five different missense mutations—cause autosomal dominant ICA by haploinsufficiency. RPSA encodes ribosomal protein SA, a component of the small subunit of the ribosome. This discovery establishes an essential role for RPSA in human spleen development

Ribosomal protein SA haploinsufficiency in humans with isolated congenital asplenia

Isolated congenital asplenia (ICA) is characterized by the absence of a spleen at birth in individuals with no other developmental defects. The patients are prone to life-threatening bacterial infections. The unbiased analysis of exomes revealed heterozygous mutations in RPSA in 18 patients from eight kindreds, corresponding to more than half the patients and over one-third of the kindreds studied. The clinical penetrance in these kindreds is complete. Expression studies indicated that the mutations carried by the patients-a nonsense mutation, a frameshift duplication, and five different missense mutations-cause autosomal dominant ICA by haploinsufficiency. RPSA encodes ribosomal protein SA, a component of the small subunit of the ribosome. This discovery establishes an essential role for RPSA in human spleen development