Supporting Pedagogical Practices Through the Interactive Learning Network (ILN)

Led by Mr. James Henri, Deputy Director of CITE, the IPPO project is co-investigated by six staff members of the Division of Information & Technology Studies. Like in the IPPO project, the seminar will be run as a team effort of the IPPO team.This seminar is co-organised with the Faculty of Education Research Office In this seminar, the speakers share with participants their experiences of implementing different online pedagogical practices using the ILN, the online learning support platform that CITE has developed. The speakers will draw on a collaborative action research project, the Innovative Pedagogical Practices Online (IPPO) project, funded by HKU SPACE Research Grant and launched early in 2003 within the Division of IT&S. Objectives of the study included investigating the learning styles of part-time students in the BEd ITE and LIS programs to enhance motivation and performance. Innovative practices were developed and trialed using the ILN platform to investigate how self-directed BEd students are in their learning. The ILN is a purpose built Content and Learner Management System allowing online delivery and access to materials. It is also a community-building environment where teachers/students work as teams and engage in reflective, collegial patterns of work. It facilitates both cognitive and social scaffolding, enabling educators and students to become progressively more involved in the community to sustain their commitment and interests. This environment is designed to support academic programs that rely heavily on pedagogies that emphasize the emergence and growth of autonomous, collaborative learning, rather than teacher-directed delivery of learning materials. This presentation will outline overall pedagogical design and showcase three examples of IPPO highlighting the ILN features used. The various approaches to integrating ILN to support pedagogy, peer and student-teacher interaction, and scaffolding will be demonstrated. Lecturers will provide an assessment of how well learning outcomes were achieved in their IPPO environments.published_or_final_versionCentre for Information Technology in Education, University of Hong Kon

Intranasal Flu Vaccine Protective against Seasonal and H5N1 Avian Influenza Infections

Background Influenza A (flu) virus causes significant morbidity and mortality worldwide, and current vaccines require annual updating to protect against the rapidly arising antigenic variations due to antigenic shift and drift. In fact, current subunit or split flu vaccines rely exclusively on antibody responses for protection and do not induce cytotoxic T (Tc) cell responses, which are broadly cross-reactive between virus strains. We have previously reported that γ-ray inactivated flu virus can induce cross-reactive Tc cell responses. Methodology/Principal Finding Here, we report that intranasal administration of purified γ-ray inactivated human influenza A virus preparations (γ-Flu) effectively induces heterotypic and cross-protective immunity. A single intranasal administration of γ-A/PR8[H1N1] protects mice against lethal H5N1 and other heterotypic infections. Conclusions/Significance Intranasal γ-Flu represents a unique approach for a cross-protective vaccine against both seasonal as well as possible future pandemic influenza A virus infections.Mohammed Alsharifi, Yoichi Furuya, Timothy R. Bowden, Mario Lobigs, Aulikki Koskinen, Matthias Regner, Lee Trinidad, David B. Boyle and Arno Müllbache

A rotating molecular disk toward IRAS 18162-2048, the exciting source of HH 80-81

We present several molecular line emission arcsec and subarcsec observations obtained with the Submillimeter Array (SMA) in the direction of the massive protostar IRAS 18162-2048, the exciting source of HH 80-81. The data clearly indicates the presence of a compact (radius~425-850 AU) SO2 structure, enveloping the more compact (radius~150 AU) 1.4 millimeter dust emission (reported in a previous paper). The emission spatially coincides with the position of the prominent thermal radio jet which terminates at the HH 80-81 and HH 80N Herbig-Haro objects. Furthermore, the molecular emission is elongated in the direction perpendicular to the axis of the thermal radio jet, suggesting a disk-like structure. We derive a total dynamic mass (disk-like structure and protostar) of 11-15 msun. The SO2 spectral line data also allow us to constrain the structure temperature between 120-160 K and the volume density > 2x10^9 cm-3. We also find that such a rotating flattened system could be unstable due to gravitational disturbances. The data from C17O line emission show a dense core within this star-forming region. Additionally, the H2CO and the SO emissions appear clumpy and trace the disk-like structure, a possible interaction between a molecular core and the outflows, and in part, the cavity walls excavated by the thermal radio jet.Comment: 13 pages,11 figures. Accepted in the AJ, 07-15-201

Clustered star formation and outflows in AFGL 2591

We report on a detailed study of the water maser kinematics and radio continuum emission toward the most massive and young object in the star-forming region AFGL 2591. Our analysis shows at least two spatial scales of multiple star formation, one projected across 0.1 pc on the sky and another one at about 2000 AU from a ZAMS star of about 38 Solar masses. This young stellar object drives a powerful jet- and wind-driven outflow system with the water masers associated to the outflow walls, previously detected as a limb-brightened cavity in the NIR band. At about 1300 AU to the north of this object a younger protostar drives two bow shocks, outlined by arc-like water maser emission, at 200 AU either side of the source. We have traced the velocity profile of the gas that expands along these arc-like maser structures and compared it with the jet-driven outflow model. This analysis suggests that the ambient medium around the northern protostar is swept up by a jet-driven shock (>66 km/s) and perhaps a lower-velocity (~10 km/s) wind with an opening angle of about 20 degrees from the jet axis.Comment: 21 pages, 4 figures, 2 tables, accepted by The Astrophysical Journa

Late Neanderthal subsistence strategies and cultural traditions in the northern Iberia Peninsula: Insights from Prado Vargas, Burgos, Spain

In order to better understand the causes and geographic patterns of Neanderthal demise it is necessary to broaden the focus of existing Neanderthal studies to include new sites from understudied regions, particularly those containing multi-level fossil and lithic records, and to improve regional-scale Neanderthal extinction frameworks using multiple dating techniques. To this end, we present an interdisciplinary study of the stratigraphy, chronology, pollen, fauna, lithic technology and human remains of the last Neanderthal level (Level N4) of Prado Vargas – a cave in northern Iberia, whose geographic location and chronology are ideal for investigating possible socio-economic and climatic influences on Neanderthal decline. Level N4 has yielded a rich Late Mousterian palimpsest indicative of repeated seasonal occupations, as well as a deciduous Neanderthal tooth, confirming the presence of children at the site. A wide range of human activities are detected in Level 4, with subsistence strategies demonstrating knowledgeable exploitation of the natural environs around the area. The site provides evidence for a distinctive recycling economy, including bone retouchers, recycling of cores, and intense (re)use of raw materials, which may reflect recurrent occupations or the particular cultural traditions of a regional group. Level N4 is dated to between 54.7 and 39.8 thousand years ago (ka) according to our new OSL and radiocarbon study. The late Neanderthal inhabitants of Prado Vargas were cold-adapted, and may have already been living in small, separate groups with marked territories and cultural traditions prior to the arrival of Homo sapiens in the Iberia Peninsula.Consejería de Cultura y Turismo de la Junta de Castilla y León y Ayuntamiento Merindad de Sotoscueva. The C14 dating was funded by Fundación Palarq. The OSL dating research was funded by Australian Research Council (ARC) Discovery Early Career Researcher Award DE160100743 and ARC Future Fellowship project FT200100816 awarded to M. Demuro. Marta Santamaría is the beneficiary of a predoctoral grant from University of Burgos (UBU). Gala Gómez Merino did tasks of cleaning and conservation of the tooth. We are grateful to Fundación La Escuela (Cornejo), Asociación Naboki (Quisicedo), Casa del Parque del Monumento Natural de Ojo Guareña and Benigno Gómez Pereda

Analysis of dietary fibre of boiled and canned legumes commonly consumed in the United Kingdom

The use of different analytical methods to measure the dietary fibre content of foods complicates the interpretation of epidemiological studies. The aim of this study was to determine the total (TDF) and insoluble (IDF) fibre content of 14 boiled and canned legumes commonly consumed in the UK using the Association of Official Analytical Chemists (AOAC) enzymatic gravimetric method. The fibre values obtained were compared to non-starch polysaccharide (NSP) values. The results showed that mean values for TDF (2.7–11.2 g/100 g) were higher than NSP (2.6–6.7 g/100 g), with a mean NSP:TDF ratio of 1:1.43. TDF was correlated with NSP (r = 0.6; p = 0.02). Canning significantly reduced TDF and IDF by an average of 30% and 26% compared to boiling respectively. However, IDF represented at least 60% of the TDF in both boiled and canned samples. In conclusion, fibre values are affected by the processing and analytical method used

Synaptic Accumulation of PSD-95 and Synaptic Function Regulated by Phosphorylation of Serine-295 of PSD-95

SummaryThe scaffold protein PSD-95 promotes the maturation and strengthening of excitatory synapses, functions that require proper localization of PSD-95 in the postsynaptic density (PSD). Here we report that phosphorylation of ser-295 enhances the synaptic accumulation of PSD-95 and the ability of PSD-95 to recruit surface AMPA receptors and potentiate excitatory postsynaptic currents. We present evidence that a Rac1-JNK1 signaling pathway mediates ser-295 phosphorylation and regulates synaptic content of PSD-95. Ser-295 phosphorylation is suppressed by chronic elevation, and increased by chronic silencing, of synaptic activity. Rapid dephosphorylation of ser-295 occurs in response to NMDA treatment that causes chemical long-term depression (LTD). Overexpression of a phosphomimicking mutant (S295D) of PSD-95 inhibited NMDA-induced AMPA receptor internalization and blocked the induction of LTD. The data suggest that synaptic strength can be regulated by phosphorylation-dephosphorylation of ser-295 of PSD-95 and that synaptic depression requires the dephosphorylation of ser-295

Quit Attempt Correlates among Smokers by Race/Ethnicity

Introduction: Cigarette smoking is the leading preventable cause of premature deaths in the U.S., accounting for approximately 443,000 deaths annually. Although smoking prevalence in recent decades has declined substantially among all racial/ethnic groups, disparities in smoking-related behaviors among racial/ethnic groups continue to exist. Two of the goals of Healthy People 2020 are to reduce smoking prevalence among adults to 12% or less and to increase smoking cessation attempts by adult smokers from 41% to 80%. Our study assesses whether correlates of quit attempts vary by race/ethnicity among adult (≥18 years) smokers in the U.S. Understanding racial/ethnic differences in how both internal and external factors affect quit attempts is important for targeting smoking-cessation interventions to decrease tobacco-use disparities. Methods: We used 2003 Tobacco Use Supplement to the Current Population Survey (CPS) data from 16,213 adults to examine whether the relationship between demographic characteristics, smoking behaviors, smoking policies and having made a quit attempt in the past year varied by race/ethnicity. Results: Hispanics and persons of multiple races were more likely to have made a quit attempt than whites. Overall, younger individuals and those with >high school education, who smoked fewer cigarettes per day and had smoked for fewer years were more likely to have made a quit attempt. Having a smoke-free home, receiving a doctor’s advice to quit, smoking menthol cigarettes and having a greater time to when you smoked your first cigarette of the day were also associated with having made a quit attempt. The relationship between these four variables and quit attempts varied by race/ethnicity; most notably receiving a doctor’s advice was not related to quit attempts among Asian American/Pacific Islanders and menthol use among whites was associated with a lower prevalence of quit attempts while black menthol users were more likely to have made a quit attempt than white non-menthol users. Conclusions: Most correlates of quit attempts were similar across all racial/ethnic groups. Therefore population-based comprehensive tobacco control programs that increase quit attempts and successful cessation among all racial/ethnic groups should be continued and expanded. Additional strategies may be needed to encourage quit attempts among less educated, older, and more addicted smokers

Identification of Novel Predictor Classifiers for Inflammatory Bowel Disease by Gene Expression Profiling

BACKGROUND: Improvement of patient quality of life is the ultimate goal of biomedical research, particularly when dealing with complex, chronic and debilitating conditions such as inflammatory bowel disease (IBD). This is largely dependent on receiving an accurate and rapid diagnose, an effective treatment and in the prediction and prevention of side effects and complications. The low sensitivity and specificity of current markers burden their general use in the clinical practice. New biomarkers with accurate predictive ability are needed to achieve a personalized approach that take the inter-individual differences into consideration. METHODS: We performed a high throughput approach using microarray gene expression profiling of colon pinch biopsies from IBD patients to identify predictive transcriptional signatures associated with intestinal inflammation, differential diagnosis (Crohn's disease or ulcerative colitis), response to glucocorticoids (resistance and dependence) or prognosis (need for surgery). Class prediction was performed with self-validating Prophet software package. RESULTS: Transcriptional profiling divided patients in two subgroups that associated with degree of inflammation. Class predictors were identified with predictive accuracy ranging from 67 to 100%. The expression accuracy was confirmed by real time-PCR quantification. Functional analysis of the predictor genes showed that they play a role in immune responses to bacteria (PTN, OLFM4 and LILRA2), autophagy and endocytocis processes (ATG16L1, DNAJC6, VPS26B, RABGEF1, ITSN1 and TMEM127) and glucocorticoid receptor degradation (STS and MMD2). CONCLUSIONS: We conclude that using analytical algorithms for class prediction discovery can be useful to uncover gene expression profiles and identify classifier genes with potential stratification utility of IBD patients, a major step towards personalized therapy

Rapid and bi-directional regulation of AMPA receptor phosphorylation and trafficking by JNK

Jun N-terminal kinases (JNKs) are implicated in various neuropathological conditions. However, physiological roles for JNKs in neurons remain largely unknown, despite the high expression level of JNKs in brain. Here, using bioinformatic and biochemical approaches, we identify the AMPA receptor GluR2L and GluR4 subunits as novel physiological JNK substrates in vitro, in heterologous cells and in neurons. Consistent with this finding, GluR2L and GluR4 associate with specific JNK signaling components in the brain. Moreover, the modulation of the novel JNK sites in GluR2L and GluR4 is dynamic and bi-directional, such that phosphorylation and de-phosphorylation are triggered within minutes following decreases and increases in neuronal activity, respectively. Using live-imaging techniques to address the functional consequence of these activity-dependent changes we demonstrate that the novel JNK site in GluR2L controls reinsertion of internalized GluR2L back to the cell surface following NMDA treatment, without affecting basal GluR2L trafficking. Taken together, our results demonstrate that JNK directly regulates AMPA-R trafficking following changes in neuronal activity in a rapid and bi-directional manner