142 research outputs found

    Influence of Tire Tread Pattern and Runway Surface Condition on Braking Friction and Rolling Resistance of a Modern Aircraft Tire

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    A series of taxiing tests was conducted at the Langley landing loads track with both braked and unbraked (freely rolling) single and tandem wheels equipped with 32x8.8 type VII aircraft tires of different tread designs to obtain data on tire and braking characteristics during operation on dry and on contaminated concrete and asphalt run ways. Contaminants used were water, slush, JP-4 jet fuel, and organic and detergent fire-extinguishing foams. Forward velocities for the tests ranged from approximately 13 to 104 knots. Vertical loads of approximately 9,000 to 22,000 pounds and tire inflation pressures of 85 to 350 pounds per square inch were used. Results indicated that the unbraked tire rolling resistance increased with increasing forward velocity on dry and on contaminated runway surfaces. Peak tire-ground friction coefficients developed during wheel braking decreased rapidly with increasing velocity on contaminated runways but remained relatively unchanged on dry runways as the forward velocity was increased. Dry-runway friction coefficients were found to be relatively insensitive to tire tread pattern. However, the magnitude of the friction coefficients developed by tires on contaminated runways was extremely sensitive to the tire tread pat tern used, with circumferential-groove treads developing the highest values of friction coefficient, and smooth and dimple treads the lowest values for the tread patterns and runway conditions investigated

    Dairy farming with reduced inductions

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    The New Zealand (NZ) dairy industry is reliant on seasonal pasture production and a concentrated calving interval to best match pasture supply and animal demand. To achieve this goal, some farmers induce lactation in late calving cows. This has animal welfare implications, which could result in non-tariff trade barriers to NZ dairy products (Blackett, Compton and Glassey, C. 2006, Stevens, J., Burton, L, Rendel, J. 2000). Additionally there are concerns with drug residues in the milk from herds where a large percentage of cows are induced. New standards were introduced in the 2010-11 season by the NZ Veterinarians Association (NZVA), Dairy NZ, Dairy Companies Association of NZ (DCANZ) and Federated Farmers. In the 2011-12 season the level of inductions within an individual herd will not exceed 8% reducing to 4% in 2012-13. There will be requirements for information about the stage of pregnancy; the age of the cow (under eight years old) and body condition score (4.5 to 6.5). Although this reduction may seem onerous, the NZVA has stated that only 3% of the national herd was induced in the season just finished, with 98% of farms being under 15% (Benny 2011). A survey of Canterbury dairy farmers in 2008 found that 36% operate a nil induction policy (Pangborn, 2008). With reduced levels of inductions farmers will be forced to adopt an eight week mating system if they are to maintain the traditional calving patterns. If the number of late calving cows cannot be reduced to fewer than 4%, then a larger number of cows will be culled. If a pregnant cow is worth 2,000andanonpregnantcow2,000 and a non-pregnant cow 500 there could be significant capital losses. The purpose of this paper is to review the basics of getting cows in calf and strategies for reduced inductions, discuss the results of the nil induction policy of the Lincoln University Dairy Farm (LUDF), and look at the plan of one Canterbury farm to meet the new guidelines

    Biomineralization of dolomite and magnesite discovered in tropical coralline algae: a biological solution to the geological dolomite problem

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    Final revised paper can be found at Description (Link) belowDolomite is a magnesium-rich carbonate mineral abundant in fossil carbonate reef platforms but surprisingly rare in modern sedimentary environments, a conundrum known as the ''Dolomite Problem". Marine sedimentary dolomite has been interpreted to form by an unconfirmed, post-depositional diagenetic process, despite minimal experimental success at replicating this. Here we show that dolomite, accompanied by magnesite, forms within living crustose coralline alga, Hydrolithon onkodes, a prolific global tropical reef species. Chemical micro-analysis of the coralline skeleton reveals that not only are the cell walls calcitised, but that cell spaces are typically filled with magnesite, rimmed by dolomite, or both. Mineralogy was confirmed by X-ray diffraction. Thus there are at least three mineral phases present (magnesium calcite, dolomite and magnesite) rather than one or two (magnesium calcite and brucite) as previously thought. Our results are consistent with dolomite occurrences in coralline algae rich environments in fossil reefs. Instead of a theory of post-depositional dolomitisation, we present evidence revealing biomineralization that can account for the massive formations seen in the geologic record. Additionally, our findings imply that previously unrecognized dolomite and magnesite have formed throughout the Holocene. This discovery together with the scale of coralline algae dominance in past shallow carbonate environments raises the possibility that environmental factors driving this biological dolomitisation process have influenced the global marine magnesium/calcium cycle. Perhaps, most importantly, we reveal that what has been considered a geological process can be a biological process, having many implications for both disciplines.M. C. Nash, U. Troitzsch, B. N. Opdyke, J. M. Trafford, B. D. Russell and D. I. Klinehttp://hdl.handle.net/2440/6855

    First discovery of dolomite and magnesite in living coralline algae and its geobiological implications

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    Dolomite is a magnesium-rich carbonate mineral abundant in fossil carbonate reef platforms but surprisingly rare in modern sedimentary environments, a conundrum known as the "Dolomite Problem". Marine sedimentary dolomite has been interpreted to form by an unconfirmed, post-depositional diagenetic process, despite minimal experimental success at replicating this. Here we show that dolomite, accompanied by magnesite, forms within living crustose coralline alga, Hydrolithon onkodes, a prolific global tropical reef species. Chemical micro-analysis of the coralline skeleton reveals that not only are the cell walls calcitised, but that cell spaces are typically filled with magnesite, rimmed by dolomite, or both. Mineralogy was confirmed by X-ray Diffraction. Thus there are at least three mineral phases present (magnesium calcite, dolomite and magnesite) rather than one or two (magnesium calcite and brucite) as previously thought. Our results are consistent with dolomite occurrences in coralline algae rich environments in fossil reefs of the last 60 million years. We reveal that the standard method of removing organic material prior to Xray Diffraction analysis can result in a decrease in the most obvious dolomite and magnesite diffraction patterns and this may explain why the abundant protodolomite and magnesite discovered in this study has not previously been recognized. This discovery of dolomite in living coralline algae extends the range of palaeo-environments for which biologically initiated dolomite can be considered a possible source of primary dolomite.M. C. Nash, U. Troitzsch, B. N. Opdyke, J. M. Trafford, B. D. Russell and D. I. Klin

    A carbohydrate-binding protein, B-GRANULE CONTENT 1, influences starch granule size distribution in a dose-dependent manner in polyploid wheat

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    In Triticeae endosperm (e.g. wheat and barley), starch granules have a bimodal size distribution (with A- and B-type granules) whereas in other grasses the endosperm contains starch granules with a unimodal size distribution. Here, we identify the gene, BGC1 (B-GRANULE CONTENT 1), responsible for B-type starch granule content in Aegilops and wheat. Orthologues of this gene are known to influence starch synthesis in diploids such as rice, Arabidopsis, and barley. However, using polyploid Triticeae species, we uncovered a more complex biological role for BGC1 in starch granule initiation: BGC1 represses the initiation of A-granules in early grain development but promotes the initiation of B-granules in mid grain development. We provide evidence that the influence of BGC1 on starch synthesis is dose dependent and show that three very different starch phenotypes are conditioned by the gene dose of BGC1 in polyploid wheat: normal bimodal starch granule morphology; A-granules with few or no B-granules; or polymorphous starch with few normal A- or B-granules. We conclude from this work that BGC1 participates in controlling B-type starch granule initiation in Triticeae endosperm and that its precise effect on granule size and number varies with gene dose and stage of development

    Dairy industry scenarios: Informing dairy farm systems for the future

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    The purpose of the Dairy Farm Systems for the Future project is to explore how best to identify and design farming systems best suited to the changing environment and farmer circumstances. The first step in the project was to describe that changing business environment to develop a better understanding by farmers, industry and researchers of possible, plausible future scenarios for dairying in New Zealand. These scenarios are now providing reliable and comprehensive information, a context, for the design and rigorous evaluation of a range of farming systems best suited to each possible, plausible future. The scenarios were developed through a series of workshops and research phases throughout 2014/15. The process involved 68 industry stakeholders (25 organisations plus farmers/farm consultants) and resulted in the development of four scenarios. The scenario development process, outlined in this report, looked beyond the current views (the base scenario) to come up with three very different futures that the dairy industry could be facing in 10 years' time. The futures reflect the interpretation of 16 specific local and global uncertainties/risks. In-depth research into the level of variation in, and the implications of, each uncertainty informed the scenario building process.DairyNZ, Ministry for Primary Industrie

    Frequency-modulated atomic force microscopy localises viscoelastic remodelling in the ageing sheep aorta

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    We gratefully acknowledge funding from the Royal Society for the provision of an International Travel Grant for Collaboration (R112205) to RA, and Wellcome Trust Value in People Award to RA and MJS. MJS and BD gratefully acknowledge the support of the Medical Research Council (www.mrc.ac.uk: grant reference G1001398)

    K201 (JTV-519) alters the spatiotemporal properties of diastolic Ca2+ release and the associated diastolic contraction during β-adrenergic stimulation in rat ventricular cardiomyocytes

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    K201 has previously been shown to reduce diastolic contractions in vivo during β-adrenergic stimulation and elevated extracellular calcium concentration ([Ca2+]o). The present study characterised the effect of K201 on electrically stimulated and spontaneous diastolic sarcoplasmic reticulum (SR)-mediated Ca2+ release and contractile events in isolated rat cardiomyocytes during β-adrenergic stimulation and elevated [Ca2+]o. Parallel experiments using confocal microscopy examined spontaneous diastolic Ca2+ release events at an enhanced spatiotemporal resolution. 1.0 μmol/L K201 in the presence of 150 nmol/L isoproterenol (ISO) and 4.75 mmol/L [Ca2+]o significantly decreased the amplitude of diastolic contractions to ~16% of control levels. The stimulated free Ca2+ transient amplitude was significantly reduced, but stimulated cell shortening was not significantly altered. When intracellular buffering was taken into account, K201 led to an increase in action potential-induced SR Ca2+ release. Myofilament sensitivity to Ca2+ was not changed by K201. Confocal microscopy revealed diastolic events composed of multiple Ca2+ waves (2–3) originating at various points along the cardiomyocyte length during each diastolic period. 1.0 μmol/L K201 significantly reduced the (a) frequency of diastolic events and (b) initiation points/diastolic interval in the remaining diastolic events to 61% and 71% of control levels respectively. 1.0 μmol/L K201 can reduce the probability of spontaneous diastolic Ca2+ release and their associated contractions which may limit the propensity for the contractile dysfunction observed in vivo

    Responsible management: Engaging moral reflexive practice through threshold concepts

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    YesIn this conceptual paper we argue that, to date, principles of responsible management have not impacted practice as anticipated because of a disconnect between knowledge and practice. This disconnect means that an awareness of ethical concerns, by itself, does not help students take personal responsibility for their actions. We suggest that an abstract knowledge of principles has to be supplemented by an engaged understanding of the responsibility of managers and leaders to actively challenge irresponsible practices. We argue that a form of moral reflexive practice drawing on an understanding of threshold concepts is central to responsible management, and provides a gateway to transformative learning. Our conceptual argument leads to implications for management and professional education

    Examination of the Effects of Heterogeneous Organization of RyR Clusters, Myofibrils and Mitochondria on Ca2+ Release Patterns in Cardiomyocytes

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    Spatio-temporal dynamics of intracellular calcium, [Ca2+]i, regulate the contractile function of cardiac muscle cells. Measuring [Ca2+]i flux is central to the study of mechanisms that underlie both normal cardiac function and calcium-dependent etiologies in heart disease. However, current imaging techniques are limited in the spatial resolution to which changes in [Ca2+]i can be detected. Using spatial point process statistics techniques we developed a novel method to simulate the spatial distribution of RyR clusters, which act as the major mediators of contractile Ca2+ release, upon a physiologically-realistic cellular landscape composed of tightly-packed mitochondria and myofibrils.We applied this method to computationally combine confocal-scale (~ 200 nm) data of RyR clusters with 3D electron microscopy data (~ 30 nm) of myofibrils and mitochondria, both collected from adult rat left ventricular myocytes. Using this hybrid-scale spatial model, we simulated reaction-diffusion of [Ca2+]i during the rising phase of the transient (first 30 ms after initiation). At 30 ms, the average peak of the simulated [Ca2+]i transient and of the simulated fluorescence intensity signal, F/F0, reached values similar to that found in the literature ([Ca2+]i 1 μM; F/F0 5.5). However, our model predicted the variation in [Ca2+]i to be between 0.3 and 12.7 μM (~3 to 100 fold from resting value of 0.1 μM) and the corresponding F/F0 signal ranging from 3 to 9.5. We demonstrate in this study that: (i) heterogeneities in the [Ca2+]i transient are due not only to heterogeneous distribution and clustering of mitochondria; (ii) but also to heterogeneous local densities of RyR clusters. Further, we show that: (iii) these structureinduced heterogeneities in [Ca2+]i can appear in line scan data. Finally, using our unique method for generating RyR cluster distributions, we demonstrate the robustness in the [Ca2+]i transient to differences in RyR cluster distributions measured between rat and human cardiomyocytes
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