Electromagnetic levitation coil fabrication technique for MSFC containerless processing facilities

A technique is described for more reproducible fabrication of electromagnetic levitation coils. A split mandrel was developed upon which the coil is wound. After fabrication the mandrel can be disassembled to remove it from the coil. Previously, a full day was required to fabricate a levitation coil and the success rate for a functional coil was only 50 percent. About eight coils may be completed in one day using the technique developed and 95 percent of them are good levitation coils

INDEPENDENT VALIDATION OF SENSOR MODELS IN THE COMMUNITY SENSOR MODEL PROGRAM

ABSTRACT As the military community continues its reliance on imagery from airborne sensors, the need to standardize sensor models for current and future sensors becomes paramount. In support of this requirement, the Community Sensor Model (CSM) program was established within the Department of Defense. Through this program, users of airborne data are provided a common interface to all essential photogrammetric functions while maintaining proprietary aspects of the sensor, through the use of shared libraries. To date, sensor model validation has ensured that all function calls return values for variables in the correct format, as specified by the Interface Control Document (ICD). The authors propose an enhanced method of validation through the use of photogrammetric processes that quantitatively evaluates a CSM's functionality on mission imagery. To support this, the validation team has developed the Generic Software Exploitation Tool (GSET) that stresses the sensor model's essential capabilities: accurate ground-to-image and image-to-ground transformations, sensor model adjustability to account for possible systematic errors, and full rigorous error propagation. One of the most critical GSET functions proposed by the authors is the photogrammetric resection. Once the sensor model is run through this test, the control and checkpoint residuals resulting from the resection will be quantitatively analyzed for modeling quality and the presence of remaining systematic errors. Results are then reported to the CSM developer as feedback and refinement of CSM development until its final delivery

Identification of proteins from Mycobacterium tuberculosis missing in attenuated Mycobacterium bovis BCG strains.

A proteome approach, combining high-resolution two-dimensional electrophoresis (2-DE) with mass spectrometry, was used to compare the cellular protein composition of two virulent strains of Mycobacterium tuberculosis with two attenuated strains of Mycobacterium bovis Bacillus Calmette-Guerin (BCG), in order to identify unique proteins of these strains. Emphasis was given to the identification of M. tuberculosis specific proteins, because we consider these proteins to represent putative virulence factors and interesting candidates for vaccination and diagnosis of tuberculosis. The genome of M. tuberculosis strain H37Rv comprises nearly 4000 predicted open reading frames. In contrast, the separation of proteins from whole mycobacterial cells by 2-DE resulted in silver-stained patterns comprising about 1800 distinct protein spots. Amongst these, 96 spots were exclusively detected either in the virulent (56 spots) or in the attenuated (40 spots) mycobacterial strains. Fifty-three of these spots were analyzed by mass spectrometry, of which 41 were identified, including 32 M. tuberculosis specific spots. Twelve M. tuberculosis specific spots were identified as proteins, encoded by genes previously reported to be deleted in M. bovis BCG. The remaining 20 spots unique for M. tuberculosis were identified as proteins encoded by genes that are not known to be missing in M. bovis BCG

Применение тонкослойной хроматографии для индентификации дифенгидрамина гидрохлорида и прокаина гидрохлорида при их совместном присутствии

ТЕХНОЛОГИЯ ФАРМАЦЕВТИЧЕСКАЯДИМЕДРОЛНОВОКАИНХРОМАТОГРАФИЯ ТОНКОСЛОЙНАЯ /ИС

Towards the proteome of the marine bacterium Rhodopirellula baltica: mapping the soluble proteins

The marine bacterium Rhodopirellula baltica, a member of the phylum Planctomycetes, has distinct morphological properties and contributes to remineralization of biomass in the natural environment. On the basis of its recently determined complete genome we investigated its proteome by 2-DE and established a reference 2-DE gel for the soluble protein fraction. Approximately 1000 protein spots were excised from a colloidal Coomassie-stained gel (pH 4-7), analyzed by MALDI-MS and identified by PMF. The non-redundant data set contained 626 distinct protein spots, corresponding to 558 different genes. The identified proteins were classified into role categories according to their predicted functions. The experimentally determined and the theoretically predicted proteomes were compared. Proteins, which were most abundant in 2-DE gels and the coding genes of which were also predicted to be highly expressed, could be linked mainly to housekeeping functions in glycolysis, tricarboxic acid cycle, amino acid biosynthesis, protein quality control and translation. Absence of predictable signal peptides indicated a localization of these proteins in the intracellular compartment, the pirellulosome. Among the identified proteins, 146 contained a predicted signal peptide suggesting their translocation. Some proteins were detected in more than one spot on the gel, indicating post-translational modification. In addition to identifying proteins present in the published sequence database for R. baltica, an alternative approach was used, in which the mass spectrometric data was searched against a maximal ORF set, allowing the identification of four previously unpredicted ORFs. The 2-DE reference map presented here will serve as framework for further experiments to study differential gene expression of R. baltica in response to external stimuli or cellular development and compartmentalization

Theoretical study of the absorption spectra of the lithium dimer

For the lithium dimer we calculate cross sections for absorption of radiation from the vibrational-rotational levels of the ground X [singlet Sigma g +] electronic state to the vibrational levels and continua of the excited A [singlet Sigma u +] and B [singlet Pi u] electronic states. Theoretical and experimental data are used to characterize the molecular properties taking advantage of knowledge recently obtained from photoassociation spectroscopy and ultra-cold atom collision studies. The quantum-mechanical calculations are carried out for temperatures in the range from 1000 to 2000 K and are compared with previous calculations and measurements.Comment: 20 pages, revtex, epsf, 6 fig

Project ThaiPASS: international outreach blending astronomy and Python

We present our outreach program, the Thailand–UK Python+Astronomy Summer School (ThaiPASS), a collaborative project comprising UK and Thai institutions and assess its impact and possible application to schools in the United Kingdom. Since its inception in 2018, the annual ThaiPASS has trained around 60 Thai high-school students in basic data handling skills using Python in the context of various astronomy topics, using current research from the teaching team. Our impact assessment of the 5 day summer schools shows an overwhelmingly positive response from students in both years, with over 80% of students scoring the activities above average in all activities but one. We use this data to suggest possible future improvements. We also discuss how ThaiPASS may inspire further outreach and engagement activities within the UK and beyond

Volunteering in a hybrid institutional and organizational environment: an emerging research agenda

Traditionally, volunteers are core participants in classic voluntary associations; however, the organizational context of volunteering has changed significantly in recent decades through the proliferation of new and hybrid settings of participation that mingle roles and rationalities of civil society, state and market. In this chapter, I examine the consequences of this organizational change for the nature and functions of volunteering by means of a literature review

Lean NOx Trap Catalysis for Lean Natural Gas Engine Applications

Distributed energy is an approach for meeting energy needs that has several advantages. Distributed energy improves energy security during natural disasters or terrorist actions, improves transmission grid reliability by reducing grid load, and enhances power quality through voltage support and reactive power. In addition, distributed energy can be efficient since transmission losses are minimized. One prime mover for distributed energy is the natural gas reciprocating engine generator set. Natural gas reciprocating engines are flexible and scalable solutions for many distributed energy needs. The engines can be run continuously or occasionally as peak demand requires, and their operation and maintenance is straightforward. Furthermore, system efficiencies can be maximized when natural gas reciprocating engines are combined with thermal energy recovery for cooling, heating, and power applications. Expansion of natural gas reciprocating engines for distributed energy is dependent on several factors, but two prominent factors are efficiency and emissions. Efficiencies must be high enough to enable low operating costs, and emissions must be low enough to permit significant operation hours, especially in non-attainment areas where emissions are stringently regulated. To address these issues the U.S. Department of Energy and the California Energy Commission launched research and development programs called Advanced Reciprocating Engine Systems (ARES) and Advanced Reciprocating Internal Combustion Engines (ARICE), respectively. Fuel efficiency and low emissions are two primary goals of these programs. The work presented here was funded by the ARES program and, thus, addresses the ARES 2010 goals of 50% thermal efficiency (fuel efficiency) and <0.1 g/bhp-hr emissions of oxides of nitrogen (NOx). A summary of the goals for the ARES program is given in Table 1-1. ARICE 2007 goals are 45% thermal efficiency and <0.015 g/bhp-hr NOx. Several approaches for improving the efficiency and emissions of natural gas reciprocating engines are being pursued. Approaches include: stoichiometric engine operation with exhaust gas recirculation and three-way catalysis, advanced combustion modes such as homogeneous charge compression ignition, and extension of the lean combustion limit with advanced ignition concepts and/or hydrogen mixing. The research presented here addresses the technical approach of combining efficient lean spark-ignited natural gas combustion with low emissions obtained from a lean NOx trap catalyst aftertreatment system. This approach can be applied to current lean engine technology or advanced lean engines that may result from related efforts in lean limit extension. Furthermore, the lean NOx trap technology has synergy with hydrogen-assisted lean limit extension since hydrogen is produced from natural gas during the lean NOx trap catalyst system process. The approach is also applicable to other lean engines such as diesel engines, natural gas turbines, and lean gasoline engines; other research activities have focused on those applications. Some commercialization of the technology has occurred for automotive applications (both diesel and lean gasoline engine vehicles) and natural gas turbines for stationary power. The research here specifically addresses barriers to commercialization of the technology for large lean natural gas reciprocating engines for stationary power. The report presented here is a comprehensive collection of research conducted by Oak Ridge National Laboratory (ORNL) on lean NOx trap catalysis for lean natural gas reciprocating engines. The research was performed in the Department of Energy's ARES program from 2003 to 2007 and covers several aspects of the technology. All studies were conducted at ORNL on a Cummins C8.3G+ natural gas engine chosen based on industry input to simulate large lean natural gas engines. Specific technical areas addressed by the research include: NOx reduction efficiency, partial oxidation and reforming chemistry, and the effects of sulfur poisons on the partial oxidation, reformer, and lean NOx trap catalysts. The initial work on NOx reduction efficiency demonstrated that NOx emissions <0.1 g/bhp-hr (the ARES goal) can be achieved with the lean NOx trap catalyst technology. Subsequent work focused on cost and size optimization and durability issues which addressed two specific ARES areas of interest to industry ('Cost of Power' and 'Availability, Reliability, and Maintainability', respectively). Thus, the research addressed the approach of the lean NOx trap catalyst technology toward the ARES goals as shown in Table 1-1

Angstrom-Scale Distance Dependence of Antenna-Enhanced Vibrational Signals

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