Coralsnake venomics: Analyses of venom gland transcriptomes and proteomes of six Brazilian taxa

Venom gland transcriptomes and proteomes of six Micrurus taxa (M. corallinus, M. lemniscatus carvalhoi, M. lemniscatus lemniscatus, M. paraensis, M. spixii spixii, and M. surinamensis) were investigated, providing the most comprehensive, quantitative data on Micrurus venom composition to date, and more than tripling the number of Micrurus venom protein sequences previously available. The six venomes differ dramatically. All are dominated by 2–6 toxin classes that account for 91–99% of the toxin transcripts. The M. s. spixii venome is compositionally the simplest. In it, three-finger toxins (3FTxs) and phospholipases A2 (PLA2s) comprise >99% of the toxin transcripts, which include only four additional toxin families at levels ≥0.1%. Micrurus l. lemniscatus venom is the most complex, with at least 17 toxin families. However, in each venome, multiple structural subclasses of 3FTXs and PLA2s are present. These almost certainly differ in pharmacology as well. All venoms also contain phospholipase B and vascular endothelial growth factors. Minor components (0.1–2.0%) are found in all venoms except that of M. s. spixii. Other toxin families are present in all six venoms at trace levels (<0.005%). Minor and trace venom components differ in each venom. Numerous novel toxin chemistries include 3FTxs with previously unknown 8- and 10-cysteine arrangements, resulting in new 3D structures and target specificities. 9-cysteine toxins raise the possibility of covalent, homodimeric 3FTxs or heterodimeric toxins with unknown pharmacologies. Probable muscarinic sequences may be reptile-specific homologs that promote hypotension via vascular mAChRs. The first complete sequences are presented for 3FTxs putatively responsible for liberating glutamate from rat brain synaptosomes. Micrurus C-type lectin-like proteins may have 6–9 cysteine residues and may be monomers, or homo- or heterodimers of unknown pharmacology. Novel KSPIs, 3× longer than any seen previously, appear to have arisen in three species by gene duplication and fusion. Four species have transcripts homologous to the nociceptive toxin, (MitTx) α-subunit, but all six species had homologs to the β-subunit. The first non-neurotoxic, non-catalytic elapid phospholipase A2s are reported. All are probably myonecrotic. Phylogenetic analysis indicates that the six taxa diverged 15–35 million years ago and that they split from their last common ancestor with Old World elapines nearly 55 million years ago. Given their early diversification, many cryptic micrurine taxa are anticipated

The Seventeenth Data Release of the Sloan Digital Sky Surveys: Complete Release of MaNGA, MaStar and APOGEE-2 Data

This paper documents the seventeenth data release (DR17) from the Sloan Digital Sky Surveys; the fifth and final release from the fourth phase (SDSS-IV). DR17 contains the complete release of the Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey, which reached its goal of surveying over 10,000 nearby galaxies. The complete release of the MaNGA Stellar Library (MaStar) accompanies this data, providing observations of almost 30,000 stars through the MaNGA instrument during bright time. DR17 also contains the complete release of the Apache Point Observatory Galactic Evolution Experiment 2 (APOGEE-2) survey which publicly releases infra-red spectra of over 650,000 stars. The main sample from the Extended Baryon Oscillation Spectroscopic Survey (eBOSS), as well as the sub-survey Time Domain Spectroscopic Survey (TDSS) data were fully released in DR16. New single-fiber optical spectroscopy released in DR17 is from the SPectroscipic IDentification of ERosita Survey (SPIDERS) sub-survey and the eBOSS-RM program. Along with the primary data sets, DR17 includes 25 new or updated Value Added Catalogs (VACs). This paper concludes the release of SDSS-IV survey data. SDSS continues into its fifth phase with observations already underway for the Milky Way Mapper (MWM), Local Volume Mapper (LVM) and Black Hole Mapper (BHM) surveys

Addendum: Aird, S.D. et al. Coralsnake Venomics: Analyses of Venom Gland Transcriptomes and Proteomes of Six Brazilian Taxa. Toxins 2017, 9(6), 187

Following publication of this paper, Dr. Daniel Dashevsky discovered to our chagrin, that the transcriptomic datasets uploaded to the DNA Databank of Japan (DDBJ) contained numerous complete 3FTx sequences that were not included in our paper.[...

Cidade e práticas urbanas: nas fronteiras incertas entre o ilegal, o informal e o ilícito The city and urban practices: in the uncertain frontiers between the illegal, the informal and the illicit

Neste artigo, pretende-se abordar o tráfico de drogas a partir de suas capilaridades no mundo social e nas tramas urbanas, tomando como "posto de observação" alguns de seus pontos de ancoramento na periferia da cidade de São Paulo. Essa é uma perspectiva descritiva (e analítica) que permite situar as práticas criminosas nas suas relações com o que poderíamos definir como a gestão das ilegalidades inscritas nos agenciamentos concretos da vida cotidiana. A rigor, esse é o foco da discussão a ser desenvolvida: as evidências de uma crescente e expansiva trama de ilegalidades (nova e velhas) entrelaçadas nas práticas urbanas, seus circuitos e redes sociais, e que são urdidas nas relações hoje redefinidas (e a serem bem compreendidas) entre o ilegal, o informal e o ilícito. Com base em resultados de pesquisa recente, o artigo pretende o exercício de "etnografia experimental" para tentar flagrar as mediações e conexões pelas quais esses deslocamentos das fronteiras do legal e do ilegal vêm se processando. São essas conexões e mediações que precisam ser bem compreendidas: é nelas que se tem uma chave para identificar e compreender a porosidade entre o legal e ilegal, e as fronteiras borradas entre o trabalho, expedientes de sobrevivência e práticas ilícitas; é nelas que se podem identificar e compreender as capilaridades do tráfico de drogas no mundo social, capilaridades urdidas nessas formas de junção e conjugação da trama urbana.<br>This article intends to approach the drug trade from the perspective of its capillary network in the social world and in urban weaves, using one of its trafficking points in São Paulo’s periphery as an "observation station". This is a descriptive (and analytic) perspective that allows us to consider criminal practices in relation to what could be defined as the illegality management, which is present in concrete every-day life administration practices. This is, indeed, the focus of the discussion: evidences of a growing and expansive mesh of illegalities (both old and new ones), their circuits and social networks, which are woven in urban practices and warped in the redefined relations among the illegal, the informal and the illicit. Based on the results of a recent research, the article aims to put an "experimental ethnography" into practice, in an attempt to find out the mediations and connections through which the movements of the boundaries between the legal and the illegal have been taking place. These connections and mediations must be well understood for they contain the key to identifying and understanding the porosity existent between the legal and the illegal, and the blurry boundaries among work, survival expedients and illicit practices. In them, one may identify and comprehend the capillary network of the drug trade in the social world, which is warped in the junctions and conjunctions of the urban weave

Contestation et subversion en prison

L’étude des formes de contestation et de subversion en prison ne constitue pas un champ nouveau. Néanmoins, ce numéro vise à renouveler ce champ à partir de travaux basés sur des recherches empiriques menées dans plusieurs pays (États-Unis, Mexique, Brésil, Belgique, Irlande, France, Burundi, Cameroun et Côté d’Ivoire). Les contributions analysent différentes modalités de résistance des prisonnier·e·s (résistance au système carcéral ou aux effets de l’incarcération), ainsi que la manière dont ces résistances affectent l’institution elle-même. Les articles montrent la diversité de ces résistances qui peuvent être individuelles ou collectives, bénéficier d’une forte médiatisation ou être clandestines et ils explorent ce que leur récit révèle du système carcéral. Struggle and subversion in prison are not new fields for prison studies. However, this issue aims to renew this field with articles based on empirical research conducted in several countries (United States, Mexico, Brazil, Belgium, Ireland, France, Burundi, Cameroon and Ivory Coast). The articles analyze different kinds of prisoners’ resistance (either they resist the prison system or how incarceration affect them) and how this resistance impacts the institution itself. The articles investigate individual and collective forms of resistance, either highly publicized or clandestine, and explore what their narratives reveal of the prison system

Ilegalismos urbanos e a cidade

Tomando como ponto de partida situações encontradas nas periferias paulistas, este artigo discute as relações redefinidas das relações entre o informal, o ilegal e o ilícito. Se é verdade que a transitividade entre o legal e ilegal, formal e informal sempre acompanhou a história de nossas cidades (e sociedade), apresenta-se hoje o desafio de construir um jogo de referência distinto do espaço conceitual que vigorava até recentemente, em grande medida regido pelo tema das chamadas incompletudes da modernidade brasileira.<br>Taking as a guideline situations found in the periphery of cities in the State of São Paulo, this article tries to redefine the notions of informal, illegal and illicit. If it is true that the interchange between legal and illegal, formal and informal has always been part of the history of our cities (and society), today the challenge is to build another kind of conceptual space, distant from the usual framework of the so-called incompleteness of Brazilian modernity