A simple plating assay for aneuploidy in sexual progeny of Neurospora crassa, and a new allele of mei-1.

We developed a simple ascospore plating assay for aneuploidy, based on identifying disomic progeny that inherit two independently selectable mtr alleles. We validated the assay using a known meiotic mutant, mei-2. We used this assay to demonstrate that elevated frequencies of aneuploidy previously reported to be associated with reduced DNA methylation were not, in fact, due to the methylation deficiencies. A new allele of the mei-1 gene was responsible for some of the high aneuploidy

Active-distributed temperature sensing to continuously quantify vertical flow in boreholes

We show how a distributed borehole flowmeter can be created from armored Fiber Optic cables with the Active-Distributed Temperature Sensing (A-DTS) method. The principle is that in a flowing fluid, the difference in temperature between a heated and unheated cable is a function of the fluid velocity. We outline the physical basis of the methodology and report on the deployment of a prototype A-DTS flowmeter in a fractured rock aquifer. With this design, an increase in flow velocity from 0.01 to 0.3 m s−1 elicited a 2.5°C cooling effect. It is envisaged that with further development this method will have applications where point measurements of borehole vertical flow do not fully capture combined spatiotemporal dynamics

One-Liners

One-Liners from P.T. Borgia, J. Irelan and E.U. Selker, and B.C. Turner and A. Fairfiel

Technical Note: Bed conduction impact on fiber optic DTS water temperature measurements

Error in Distributed Temperature Sensor (DTS) water temperature measurements may be introduced by contact of the fiber optic cable sensor with bed materials (e.g., seafloor, lakebed, stream bed). Heat conduction from the bed materials can affect cable temperature and the resulting DTS measurements. In the Middle Fork John Day River, apparent water temperature measurements were influenced by cable sensor contact with aquatic vegetation and fine sediment bed materials. Affected cable segments measured a diurnal temperature range reduced by 10% and lagged by 20–40 min relative to that of ambient stream temperature. The diurnal temperature range deeper within the vegetation–sediment bed material was reduced 70% and lagged 240min relative to ambient stream temperature. These site-specific results illustrate the potential magnitude of bed-conduction impacts with buried DTS measurements. Researchers who deploy DTS for water temperature monitoring should understand the importance of the environment into which the cable is placed on the range and phase of temperature measurements.This discussion paper has been under review for the journal Geoscientific Instrumentation, Methods and Data Systems (GI). Please refer to the corresponding final paper in GI. The published article is copyrighted by the author(s) and published by Copernicus Publications on behalf of the European Geosciences Union. The published article can be found at: http://www.geoscientific-instrumentation-methods-and-data-systems.net/ The final revised paper is available at: http://hdl.handle.net/1957/5657

Characterizing groundwater flow and heat transport in fractured rock using Fiber-Optic Distributed Temperature Sensing

International audienceWe show how fully distributed space-time measurements with Fiber-Optic Distributed Temperature Sensing (FO-DTS) can be used to investigate groundwater flow and heat transport in fractured media. Heat injection experiments are combined with temperature measurements along fiber-optic cables installed in boreholes. Thermal dilution tests are shown to enable detection of cross-flowing fractures and quantification of the cross flow rate. A cross borehole thermal tracer test is then analyzed to identify fracture zones that are in hydraulic connection between boreholes and to estimate spatially distributed temperature breakthrough in each fracture zone. This provides a significant improvement compared to classical tracer tests, for which concentration data are usually integrated over the whole abstraction borehole. However, despite providing some complementary results, we find that the main contributive fracture for heat transport is different to that for a solute tracer

Bed conduction impact on fiber optic distributed temperature sensing water temperature measurements

Error in distributed temperature sensing (DTS) water temperature measurements may be introduced by contact of the fiber optic cable sensor with bed materials (e.g., seafloor, lakebed, streambed). Heat conduction from the bed materials can affect cable temperature and the resulting DTS measurements. In the Middle Fork John Day River, apparent water temperature measurements were influenced by cable sensor contact with aquatic vegetation and fine sediment bed materials. Affected cable segments measured a diurnal temperature range reduced by 10%and lagged by 20–40 min relative to that of ambient stream temperature. The diurnal temperature range deeper within the vegetation–sediment bed material was reduced 70% and lagged 240 min relative to ambient stream temperature. These site-specific results illustrate the potential magnitude of bed-conduction impacts with buried DTS measurements. Researchers who deploy DTS for water temperature monitoring should understand the importance of the environment into which the cable is placed on the range and phase of temperature measurements.This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Copernicus Publications on behalf of the European Geosciences Union. The published article can be found at: http://www.geoscientific-instrumentation-methods-and-data-systems.net

Thermal-Plume fibre Optic Tracking (T-POT) test for flow velocity measurement in groundwater boreholes

International audienceWe develop an approach for measuring in-well fluid velocities using point electrical heating combined with spatially and temporally continuous temperature monitoring using Distributed Temperature Sensing (DTS). The method uses a point heater to warm a discrete volume of water. The rate of advection of this plume, once the heating is stopped, equates to the average flow velocity in the well. We conducted Thermal-Plume fibre Optic Tracking (T-POT) tests in a borehole in a fractured rock aquifer with the heater at the same depth and multiple pumping rates. Tracking of the thermal plume peak allowed the spatially varying velocity to be estimated up to 50 m downstream from the heating point, depending on the pumping rate. The T-POT technique can be used to estimate the velocity throughout long intervals provided that thermal dilution due to inflows, dispersion, or cooling by conduction do not render the thermal pulse unresolvable with DTS. A complete flow log may be obtained by deploying the heater at multiple depths, or with multiple point heaters

Impact of Technetium-99m Sestamibi Imaging on the Emergency Department Management and Costs in the Evaluation of Low-risk Chest Pain

Objectives: To assess the impact of rest sestamibi scanning on emergency physicians' (EPs') diagnostic certainty and decision making (as assessed by the hypothetical disposition of patients) for 69 consenting stable patients with suspected acute cardiac ischemia and nondiagnostic electrocardiograms. The resultant impact on costs was examined as a secondary outcome. Methods: Patients with suspected acute cardiac ischemia were injected with 25 mCi of sestamibi within two hours of active pain in one of three emergency department study sites. The probability of acute myocardial infarction (AMI) and unstable angina (UA), and hypothetical disposition decisions were recorded immediately before and after physicians were notified of scan results. Changes in disposition were classified as optimal or suboptimal. For the cost determinations, a cost-based decision support program was used. Results: For the subgroup found to be free of acute cardiac events (ACEs) ( n = 62), the EPs' post-sestamibi scan probabilities for AMI decreased by 11% and UA by 18% (p < 0.001 for both conditions). In seven patients with ACEs, the post-scan probabilities of AMI and UA increased, but neither was statistically significant. Scan results led to hypothetical disposition changes in 29 patients (42%), of which 27 (93%) were optimal (nine patients were reassigned to a lower level of care, two to a higher level, and 16 additional patients to “discharge-home” status). The strategy of scanning all patients who were low to moderate risk for acute cardiac ischemia would result in an increase of direct costs of care of $222 per patient evaluated, due to added cost of sestamibi scanning. Conclusions: Sestamibi scanning results appropriately affected the EPs' estimates of the probability of AMI and UA and improved disposition decisions. Scanning all low-risk patients would likely be associated with increased costs at this medical center.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73784/1/j.1553-2712.2001.tb02108.x.pd

Probabilistic classification of acute myocardial infarction from multiple cardiac markers

Logistic regression and Gaussian mixture model (GMM) classifiers have been trained to estimate the probability of acute myocardial infarction (AMI) in patients based upon the concentrations of a panel of cardiac markers. The panel consists of two new markers, fatty acid binding protein (FABP) and glycogen phosphorylase BB (GPBB), in addition to the traditional cardiac troponin I (cTnI), creatine kinase MB (CKMB) and myoglobin. The effect of using principal component analysis (PCA) and Fisher discriminant analysis (FDA) to preprocess the marker concentrations was also investigated. The need for classifiers to give an accurate estimate of the probability of AMI is argued and three categories of performance measure are described, namely discriminatory ability, sharpness, and reliability. Numerical performance measures for each category are given and applied. The optimum classifier, based solely upon the samples take on admission, was the logistic regression classifier using FDA preprocessing. This gave an accuracy of 0.85 (95% confidence interval: 0.78–0.91) and a normalised Brier score of 0.89. When samples at both admission and a further time, 1–6 h later, were included, the performance increased significantly, showing that logistic regression classifiers can indeed use the information from the five cardiac markers to accurately and reliably estimate the probability AMI

Caracterizacion de la alimentacion de verano en los sistemas ganaderos de la comunidad de Jacuapa Centro, Municipio de Matagalpa en el primer semestre 2017

Ésta investigación se realizó en Jucuapa Centro, Matagalpa, Nicaragua, en el primer semestre 2017, con el objetivo de caracterizar la alimentación de verano en los sistemas ganaderos. La presente investigación es de carácter descriptivo, de enfoque mixto cuali-cuantitativo. El universo está constituido por 15 fincas, siendo esta la muestra. Se realizó visitas, se aplicó encuesta a los productores pecuarios para obtención de los datos de interés, encontrando que los sistemas ganaderos están conformados por pequeños productores de autoconsumo que poseen como media entre 2 a 5 cabezas de ganado por finca, siendo los registro máximos de más de 11 cabeza, los sistemas se encuentran conformado por vacas lactantes, terneras y vacas preñadas con el objetivo de producción lechera, utilizando razas de doble propósito: Pardo con Brahman los que se encuentran en condición corporal normal. El tipo de explotación es extensiva sobre pasturas naturales las que en esta época del año son de mala calidad, las alternativas de alimentación de verano consisten en la compra de pacas y el almacenamiento de heno tradicional (guates) con suministro de sal común y vitaminas. Hacen usos de árboles forrajeros pero en su mayoría son consumidos de manera voluntaria en los potrero, sin un manejo sobre ellos, llegando a la conclusión que los productores de Jucuapa Centro carecen de tecnologías de manejo apropiadas para alimentar el hato en la época seca lo que ocasiona incremento en los costos de producción y la baja en los rendimientos productivos y económicos Palabras clave: Sistemas ganaderos, manejo alimentario, alimentación de veran