Sex and β-Endorphin Influence the Effects of Ethanol on Limbic Gabra2 Expression in a Mouse Binge Drinking Model

Binge drinking is a widespread problem linked to increased risk for alcohol-related complications, including development of alcohol use disorders. In the last decade, binge drinking has increased significantly, specifically in women. Clinically, sexually dimorphic effects of alcohol are well-characterized, however, the underlying mechanisms for these dimorphisms in the physiological and behavioral effects of alcohol are poorly understood. Among its many effects, alcohol consumption reduces anxiety via the inhibitory neurotransmitter GABA, most likely acting upon receptors containing the α-2 subunit (Gabra2). Previous research from our laboratory indicates that female mice lacking the endogenous opioid peptide β-endorphin (βE) have an overactive stress axis and enhanced anxiety-like phenotype, coupled with increased binge-like alcohol consumption. Because βE works via GABA signaling to reduce anxiety, we sought to determine whether sexually dimorphic binge drinking behavior in βE deficient mice is coupled with differences in CNS Gabra2 expression. To test this hypothesis, we used βE knock-out mice in a drinking in the dark model where adult male and female C57BL/6J controls (βE +/+) and βE deficient (βE -/-; B6.129S2-Pomctm1Low/J) mice were provided with one bottle of 20% ethanol (EtOH) and one of water (EtOH drinkers) or two bottles of water (water drinkers) 3 h into the dark cycle for four consecutive days. Following a binge test on day 4, limbic tissue was collected and frozen for subsequent qRT-PCR analysis of Gabra2 mRNA expression. Water-drinking βE +/+ females expressed more Gabra2 in central nucleus of the amygdala and the bed nucleus of the stria terminalis than males, but this sex difference was absent in the βE -/- mice. Genotype alone had no effect on alcohol consumption or drug-induced increase in Gabra2 expression. In contrast, βE expression had bi-directional effects in females: in wildtypes, Gabra2 mRNA was reduced by binge EtOH consumption, while EtOH increased expression in βE -/- females to levels commensurate with drug-naïve βE +/+ females. These results support the contention that βE plays a role in sexually dimorphic binge-like EtOH consumption, perhaps through differential expression of GABAA α2 subunits in limbic structures known to play key roles in the regulation of stress and anxiety

Comparing impacts of alien plants and animals in Europe using a standard scoring system

© 2015 British Ecological Society. Alien species can change the recipient environment in various ways, and some of them cause considerable damage. Understanding such impacts is crucial to direct management actions. This study addresses the following questions: Is it possible to quantify impact across higher taxa in a comparative manner? Do impacts differ between taxonomic groups? How are environmental and socio-economic impacts related? Can impacts be predicted based on those in other regions? To address these questions, we reviewed literature describing the impacts of 300 species from five major taxonomic groups: mammals, birds, fish, terrestrial arthropods and plants. To make very diverse impact measures comparable, we used the semi-quantitative generic impact scoring system (GISS) which describes environmental and socio-economic impacts using twelve categories. In each category, scores range from zero (no impact known or detectable) to five (the highest possible impact). Using the same scoring system for taxa as diverse as invertebrates, vertebrates and plants, we found that overall, alien mammals in Europe have the highest impact, while fish have the lowest. Terrestrial arthropods were found to have the lowest environmental impact, while fish had relatively low socio-economic impact. Overall, the magnitude of environmental and socio-economic impacts of individual alien species is highly correlated. However, at the species level, major deviations are found. For mammals and birds, the impacts in invaded ranges outside of Europe are broadly similar to those recorded for alien species within Europe, indicating that a consideration of the known impacts of a species in other regions can be generally useful when predicting the impacts of an alien species. However, it should be noted that this pattern is not consistent across all mammal and bird orders, and thus, such information should be considered with caution. Synthesis and applications. Comparing the impacts of alien species across taxa is necessary for prioritizing management efforts and effective allocation of resources. By applying the generic impact scoring system (GISS) to five major taxonomic groups, we provide the basis for a semi-quantitative cross-taxa listing process (e.g. 'black lists' or 100-worst-lists). If more data are collated from different geographical regions and habitats using standard GISS protocols, risk assessments for alien species based on rigorous measures of impact could be improved by taking into account local variation, and context dependence of impacts. This would also allow studies at lower taxonomic levels, and within-taxon analyses of functional groups and guilds. Comparing the impacts of alien species across taxa is necessary for prioritizing management efforts and effective allocation of resources. By applying the generic impact scoring system (GISS) to five major taxonomic groups, we provide the basis for a semi-quantitative cross-taxa listing process (e.g. 'black lists' or 100-worst-lists). If more data are collated from different geographical regions and habitats using standard GISS protocols, risk assessments for alien species based on rigorous measures of impact could be improved by taking into account local variation, and context dependence of impacts. This would also allow studies at lower taxonomic levels, and within-taxon analyses of functional groups and guilds.Peer Reviewe

Sex and β-Endorphin Influence the Effects of Ethanol on Limbic Gabra2 Expression in a Mouse Binge Drinking Model

Binge drinking is a widespread problem linked to increased risk for alcohol-related complications, including development of alcohol use disorders. In the last decade, binge drinking has increased significantly, specifically in women. Clinically, sexually dimorphic effects of alcohol are well-characterized, however, the underlying mechanisms for these dimorphisms in the physiological and behavioral effects of alcohol are poorly understood. Among its many effects, alcohol consumption reduces anxiety via the inhibitory neurotransmitter GABA, most likely acting upon receptors containing the α-2 subunit (Gabra2). Previous research from our laboratory indicates that female mice lacking the endogenous opioid peptide β-endorphin (βE) have an overactive stress axis and enhanced anxiety-like phenotype, coupled with increased binge-like alcohol consumption. Because βE works via GABA signaling to reduce anxiety, we sought to determine whether sexually dimorphic binge drinking behavior in βE deficient mice is coupled with differences in CNS Gabra2 expression. To test this hypothesis, we used βE knock-out mice in a “drinking in the dark” model where adult male and female C57BL/6J controls (βE +/+) and βE deficient (βE -/-; B6.129S2-Pomctm1Low/J) mice were provided with one bottle of 20% ethanol (EtOH) and one of water (EtOH drinkers) or two bottles of water (water drinkers) 3 h into the dark cycle for four consecutive days. Following a binge test on day 4, limbic tissue was collected and frozen for subsequent qRT-PCR analysis of Gabra2 mRNA expression. Water-drinking βE +/+ females expressed more Gabra2 in central nucleus of the amygdala and the bed nucleus of the stria terminalis than males, but this sex difference was absent in the βE -/- mice. Genotype alone had no effect on alcohol consumption or drug-induced increase in Gabra2 expression. In contrast, βE expression had bi-directional effects in females: in wildtypes, Gabra2 mRNA was reduced by binge EtOH consumption, while EtOH increased expression in βE -/- females to levels commensurate with drug-naïve βE +/+ females. These results support the contention that βE plays a role in sexually dimorphic binge-like EtOH consumption, perhaps through differential expression of GABAA α2 subunits in limbic structures known to play key roles in the regulation of stress and anxiety

Do contaminants originating from state-of-the-art treated wastewater impact the ecological quality of surface waters?

Since the 1980s, advances in wastewater treatment technology have led to considerably improved surface water quality in the urban areas of many high income countries. However, trace concentrations of organic wastewater-associated contaminants may still pose a key environmental hazard impairing the ecological quality of surface waters. To identify key impact factors, we analyzed the effects of a wide range of anthropogenic and environmental variables on the aquatic macroinvertebrate community. We assessed ecological water quality at 26 sampling sites in four urban German lowland river systems with a 0–100% load of state-of-the-art biological activated sludge treated wastewater. The chemical analysis suite comprised 12 organic contaminants (five phosphor organic flame retardants, two musk fragrances, bisphenol A, nonylphenol, octylphenol, diethyltoluamide, terbutryn), 16 polycyclic aromatic hydrocarbons, and 12 heavy metals. Non-metric multidimensional scaling identified organic contaminants that are mainly wastewater-associated (i.e., phosphor organic flame retardants, musk fragrances, and diethyltoluamide) as a major impact variable on macroinvertebrate species composition. The structural degradation of streams was also identified as a significant factor. Multiple linear regression models revealed a significant impact of organic contaminants on invertebrate populations, in particular on Ephemeroptera, Plecoptera, and Trichoptera species. Spearman rank correlation analyses confirmed wastewater-associated organic contaminants as the most significant variable negatively impacting the biodiversity of sensitive macroinvertebrate species. In addition to increased aquatic pollution with organic contaminants, a greater wastewater fraction was accompanied by a slight decrease in oxygen concentration and an increase in salinity. This study highlights the importance of reducing the wastewater-associated impact on surface waters. For aquatic ecosystems in urban areas this would lead to: (i) improvement of the ecological integrity, (ii) reduction of biodiversity loss, and (iii) faster achievement of objectives of legislative requirements, e.g., the European Water Framework Directive

Spider conservation in Europe : a review

Despite their ecological importance and diversity, spiders (Arachnida: Araneae) are underrepresented in conservation policies in comparison to other groups. We review all extant conservation tools focusing on spiders in Europe, highlighting general patterns, limitations, gaps, and future directions. We assembled a comprehensive online database reporting all available information concerning the legal protection and conservation status of 4,154 spider species. Existing international legislation has limited coverage, with only one species listed in the Bern Convention and EU Habitats Directive. At the national and subnational levels, 178 species are formally mentioned in the legislation of 19 European countries. Moreover, the International Union for Conservation of Nature (IUCN) includes assessments for 301 species worldwide, 164 of these threatened and eight native to Europe. In addition, spiders are mentioned in Regional Red Lists and Red Books in 28 out of 42 European countries considered in this review. Northern and Central European countries have the highest percentage of species assessed at the regional level in Red Lists and Red Books. The Mediterranean basin has the highest spider diversities in Europe but conservation efforts are lacking, both in terms of assessments and national or subnational legislation. Among European species, Dolomedes plantarius, Argyroneta aquatica and Eresus kollari are the most frequently mentioned in European conservation measures, possibly due to their ecological traits and their strict association with declining habitats. Considering the current threats to spiders in Europe, the protection of large areas of suitable habitat should be considered as the most effective approach to spider conservation.Peer reviewe

Towards establishment of a centralized spider traits database

A main goal of ecological and evolutionary biology is understanding and predicting interactions between populations and both abiotic and biotic environments, the spatial and temporal variation of these interactions, and the effects on population dynamics and performance. Trait-based approaches can help to model these interactions and generate a comprehensive understanding of ecosystem functioning. A central tool is the collation of databases that include species trait information. Such centralized databases have been set up for a number of organismal groups but is lacking for one of the most important groups of predators in terrestrial ecosystems - spiders. Here we promote the collation of an open spider traits database, integrated into the global Open Traits Network. We explore the current collation of spider data and cover the logistics of setting up a global database, including which traits to include, the source of data, how to input data, database governance, geographic cover, accessibility, quality control and how to make the database sustainable long-term. Finally, we explore the scope of research questions that could be investigated using a global spider traits database.Peer reviewe

Pharmaceutical Formulation Facilities as Sources of Opioids and Other Pharmaceuticals to Wastewater Treatment Plant Effluents

Facilities involved in the manufacture of pharmaceutical products are an under-investigated source of pharmaceuticals to the environment. Between 2004 and 2009, 35 to 38 effluent samples were collected from each of three wastewater treatment plants (WWTPs) in New York and analyzed for seven pharmaceuticals including opioids and muscle relaxants. Two WWTPs (NY2 and NY3) receive substantial flows (>20% of plant flow) from pharmaceutical formulation facilities (PFF) and one (NY1) receives no PFF flow. Samples of effluents from 23 WWTPs across the United States were analyzed once for these pharmaceuticals as part of a national survey. Maximum pharmaceutical effluent concentrations for the national survey and NY1 effluent samples were generally <1 μg/L. Four pharmaceuticals (methadone, oxycodone, butalbital, and metaxalone) in samples of NY3 effluent had median concentrations ranging from 3.4 to >400 μg/L. Maximum concentrations of oxycodone (1700 μg/L) and metaxalone (3800 μg/L) in samples from NY3 effluent exceeded 1000 μg/L. Three pharmaceuticals (butalbital, carisoprodol, and oxycodone) in samples of NY2 effluent had median concentrations ranging from 2 to 11 μg/L. These findings suggest that current manufacturing practices at these PFFs can result in pharmaceuticals concentrations from 10 to 1000 times higher than those typically found in WWTP effluents

Using structured eradication feasibility assessment to prioritize the management of new and emerging invasive alien species in Europe

Prioritizing the management of invasive alien species (IAS) is of global importance and within Europe integral to the EU IAS regulation. To prioritize management effectively, the risks posed by IAS need to be assessed, but so too does the feasibility of their management. While the risk of IAS to the EU has been assessed, the feasibility of management has not. We assessed the feasibility of eradicating 60 new (not yet established) and 35 emerging (established with limited distribution) species that pose a threat to the EU, as identified by horizon scanning. The assessment was carried out by 34 experts in invasion management from across Europe, applying the Non‐Native Risk Management scheme to defined invasion scenarios and eradication strategies for each species, assessing the feasibility of eradication using seven key risk management criteria. Management priorities were identified by combining scores for risk (derived from horizon scanning) and feasibility of eradication. The results show eradication feasibility score and risk score were not correlated, indicating that risk management criteria evaluate different information than risk assessment. In all, 17 new species were identified as particularly high priorities for eradication should they establish in the future, whereas 14 emerging species were identified as priorities for eradication now. A number of species considered highest priority for eradication were terrestrial vertebrates, a group that has been the focus of a number of eradication attempts in Europe. However, eradication priorities also included a diverse range of other taxa (plants, invertebrates and fish) suggesting there is scope to broaden the taxonomic range of attempted eradication in Europe. We demonstrate that broad scale structured assessments of management feasibility can help prioritize IAS for management. Such frameworks are needed to support evidence‐based decision‐making

Biodiversity and structure of spider communities along a metal pollution gradient

The objective of the study was to determine whether long-term metal pollution affects communities of epigeal spiders (Aranea), studied at three taxonomic levels: species, genera, and families. Biodiversity was defined by three indices: the Hierarchical Richness Index (HRI), Margalef index (DM) and Pielou evenness index (J). In different ways the indices describe taxa richness and the distribution of individuals among taxa. The dominance pattern of the communities was described with four measures: number of dominant species at a site, percentage of dominant species at a site, average dominant species abundance at a site, and the share of the most numerous species (Alopecosa cuneata) at a site. Spiders were collected along a metal pollution gradient in southern Poland, extending ca. 33 km from zinc and lead smelter to an uncontaminated area. The zinc concentration in soil was used as the pollution index.The study revealed a significant effect of metal pollution on spider biodiversity as described by HRI for species (p = 0.039), genera (p = 0.0041) and families (p = 0.0147), and by DM for genera (p = 0.0259) and families (p = 0.0028). HRI correlated negatively with pollution level, while DM correlated positively. This means that although broadly described HRI diversity decreased with increasing pollution level, species richness increased with increasing contamination. Mesophilic meadows were generally richer. Pielou (J) did not show any significant correlations. There were a few evidences for the intermediate disturbance hypothesis: certain indices reached their highest values at moderate pollution levels rather than at the cleanest or most polluted sites