European rodent on the edge: status and distribution of the Vojvodina blind mole rat

Recent research of blind mole rats of the species complex Nannospalax (superspecies leucodon) identified a small and fragmented population of these rodents on both sides of the Hungarian-Serbian border. Cytogenetic investigations proved that this population karyologically identical with the Vojvodina blind mole rat described earlier as Nannospalax (leucodon) montanosyrmiensis. Based on cytochrome b gene sequences obtained from three specimens originating from separate locations, these blind mole rats form a discrete phylogenetic clade which, with a difference of about 10%, is well separated from other blind mole rat taxa inhabiting the Carpathian Basin. The taxon has only two extant populations that are 150 km apart from each other. The combined occupied area is estimated to be less than 10 km(2), and the total estimated number of individuals is less than 300. These two remaining populations are heavily fragmented and under imminent threat by the establishment of tree plantations, small-scale and agro-industrial farms and land development. The situation is further aggravated by the fact that 80% of the individuals inhabit unprotected areas. A study of the landscape history of the wider area surrounding one of the populations - based on military maps spanning over the last 200 years - has shown a drastic decrease in the extent and quality of potential habitats. Based on our present knowledge, the Vojvodina blind mole rat is one of the most seriously threatened, rarest mammal in Europe, the remaining population of which can be wiped out within years unless immediate conservation action is taken. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-2-2) contains supplementary material, which is available to authorized users

Supplementary data for the article: Marković, J. M.; Trišović, N. P.; Tóth-Katona, T.; Milčić, M. K.; Marinković, A. D.; Zhang, C.; Jákli, A. J.; Fodor-Csorba, K. A Structure-Property Relationship Study of Bent-Core Mesogens with Pyridine as the Central Unit. New Journal of Chemistry 2014, 38 (4), 1751–1760. https://doi.org/10.1039/c3nj01430d

Supplementary material for: [https://doi.org/10.1039/c3nj01430d]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1518

Comparison of biological and chemical properties of arable and pasture Solonetz soils

Soil samples were collected from salt-affected soils (Solonetz) under different land uses, namely arable (SnA) and pasture (SnP), to investigate the effects of land use on microbiological [basal soil respiration (BSR), microbial biomass carbon (MBC), dehydrogenase activity (DHA) and phosphatase activity] and chemical properties [organic carbon (OC), humic ratio (E4/E6), pH, electrical conductivity (EC), ammonium nitrogen (NH4-N), nitrate nitrogen (NO3-N), available forms of phosphorus (P2O5), potassium (K2O), calcium (Ca2+), magnesium (Mg2+), sodium (Na+)] and on the moisture content. The results showed that the two sites, SnA and SnP, were statistically different from each other for all the microbiological and chemical parameters investigated except Na+ and moisture content. Higher values of MBC (575.67 μg g-1), BSR (9.71 μg CO2 g-1 soil h-1), DHA (332.76 μg formazan g-1 day-1) and phosphatase activity (0.161 μmol PNP g-1 hr-1) were observed for the SnP soil. Great heterogeneity was found in SnP in terms of microbiological properties, whereas the SnA plots showed more homogeneous microbiological activity due to ploughing. 75.34% of variance was explained by principal component one (PC1), which significantly separated SnA and SnP, especially on the basis of soil MBC and P2O5. Moreover, it was concluded that the pasture land (SnP) was microbiologically more active than arable land (SnA) among the Hungarian salt-affected soils investigated

Asteroseismology of the Beta Cephei star 12 (DD) Lacertae: photometric observations, pulsational frequency analysis and mode identification

We report a multisite photometric campaign for the Beta Cephei star 12 Lacertae. 750 hours of high-quality differential photoelectric Stromgren, Johnson and Geneva time-series photometry were obtained with 9 telescopes during 190 nights. Our frequency analysis results in the detection of 23 sinusoidal signals in the light curves. Eleven of those correspond to independent pulsation modes, and the remainder are combination frequencies. We find some slow aperiodic variability such as that seemingly present in several Beta Cephei stars. We perform mode identification from our colour photometry, derive the spherical degree l for the five strongest modes unambiguously and provide constraints on l for the weaker modes. We find a mixture of modes of 0 <= l <= 4. In particular, we prove that the previously suspected rotationally split triplet within the modes of 12 Lac consists of modes of different l; their equal frequency splitting must thus be accidental. One of the periodic signals we detected in the light curves is argued to be a linearly stable mode excited to visible amplitude by nonlinear mode coupling via a 2:1 resonance. We also find a low-frequency signal in the light variations whose physical nature is unclear; it could be a parent or daughter mode resonantly coupled. The remaining combination frequencies are consistent with simple light-curve distortions. The range of excited pulsation frequencies of 12 Lac may be sufficiently large that it cannot be reproduced by standard models. We suspect that the star has a larger metal abundance in the pulsational driving zone, a hypothesis also capable of explaining the presence of Beta Cephei stars in the LMC.Comment: 12 pages, 7 figures, MNRAS, in pres

Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants

RNA silencing is one of the main defense mechanisms employed by plants to fight viruses. In change, viruses have evolved silencing suppressor proteins to neutralize antiviral silencing. Since the endogenous and antiviral functions of RNA silencing pathway rely on common components, it was suggested that viral suppressors interfere with endogenous silencing pathway contributing to viral symptom development. In this work, we aimed to understand the effects of the tombusviral p19 suppressor on endogenous and antiviral silencing during genuine virus infection. We showed that ectopically expressed p19 sequesters endogenous small RNAs (sRNAs) in the absence, but not in the presence of virus infection. Our presented data question the generalized model in which the sequestration of endogenous sRNAs by the viral suppressor contributes to the viral symptom development. We further showed that p19 preferentially binds the perfectly paired ds-viral small interfering RNAs (vsiRNAs) but does not select based on their sequence or the type of the 5’ nucleotide. Finally, co-immunoprecipitation of sRNAs with AGO1 or AGO2 from virus-infected plants revealed that p19 specifically impairs vsiRNA loading into AGO1 but not AGO2. Our findings, coupled with the fact that p19-expressing wild type Cymbidium ringspot virus (CymRSV) overcomes the Nicotiana benthamiana silencing based defense killing the host, suggest that AGO1 is the main effector of antiviral silencing in this host-virus combination

Supplementary data for the article: Marković, J. M.; Trišović, N. P.; Tóth-Katona, T.; Milčić, M. K.; Marinković, A. D.; Zhang, C.; Jákli, A. J.; Fodor-Csorba, K. A Structure-Property Relationship Study of Bent-Core Mesogens with Pyridine as the Central Unit. New Journal of Chemistry 2014, 38 (4), 1751–1760. https://doi.org/10.1039/c3nj01430d

Supplementary material for: [https://doi.org/10.1039/c3nj01430d]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1518