2,276 research outputs found

    Locality and Accessibility in Wh-Questions

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    Even in relatively configurational languages, such as English, speakers frequently have a choice between different constituent orders. Many of these word order variations have been linked to complexity (Hawkins 2005; inter alia). For example, heavy-NP shift is more likely if the shifted NP is more complex than the NP it shifts over (Wasow 1997). Other cases of word order variations, however, have not been considered in these terms. The choice between different wh-phrase orders, as in (1), has been said to be determined by (categorical) grammatical constraints, such as Superiorit

    On the X-ray lines in the afterglows of GRBs

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    The observation of X-ray lines in the afterglow of GRB 011211 has been reported, and challenged. The lines were interpreted as blue-shifted X-rays characteristic of a set of photoionized ``metals'', located in a section of a supernova shell illuminated by a GRB emitted a couple of days after the supernova explosion. We show that the most prominent reported lines coincide with the ones predicted in the ``cannonball'' model of GRBs. In this model, the putative signatures are Hydrogen lines, boosted by the (highly relativistic) motion of the cannonballs (CBs). The corresponding Doppler boost can be extracted from the fit to the observed I-, R- and V-band light-curves of the optical afterglow of GRB 011211, so that, since the redshift is also known, the line energies are --in the CB model-- predicted. We also discuss other GRBs of known redshift which show spectral features generally interpreted as Fe lines, or Fe recombination edges. The ensemble of results is very encouraging from the CB-model's point of view, but the data on each individual GRB are not good enough to draw (any) objectively decisive conclusions. We outline a strategy for X-ray observers to search for lines which, in the CB model, move predictably from higher to lower energies.Comment: Accepted for publication in Ap

    Optimum Small Optical Beam Displacement Measurement

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    We derive the quantum noise limit for the optical beam displacement of a TEM00 mode. Using a multimodal analysis, we show that the conventional split detection scheme for measuring beam displacement is non-optimal with 80% efficiency. We propose a new displacement measurement scheme that is optimal for small beam displacement. This scheme utilises a homodyne detection setup that has a TEM10 mode local oscillator. We show that although the quantum noise limit to displacement measurement can be surpassed using squeezed light in appropriate spatial modes for both schemes, the TEM10 homodyning scheme out-performs split detection for all values of squeezing.Comment: 13 pages, 7 figure

    REACTIVITY OF CHLOROPHYLL a/b-PROTEINS AND MICELLAR TRITON X-100 COMPLEXES OF CHLOROPHYLLS a OR b WITH BOROHYDRIDE

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    The reaction of several plant chlorophyll-protein complexes with NaBH4 has been studied by absorption spectroscopy. In all the complexes studied, chlorophyll b is more reactive than Chi a, due to preferential reaction of its formyl substituent at C-7. The complexes also show large variations in reactivity towards NaBH4 and the order of reactivity is: LHCI > PSII complex > LHCII > PSI > P700 (investigated as a component of PSI). Differential pools of the same type of chlorophyll have been observed in several complexes. Parallel work was undertaken on the reactivity of micellar complexes of chlorophyll a and of chlorophyll b with NaBH4 to study the effect of aggregation state on this reactivity. In these complexes, both chlorophyll a and b show large variations in reactivity in the order monomer > oligomer > polymer with chlorophyll b generally being more reactive than chlorophyll a. It is concluded that aggregation decreases the reactivity of chlorophylls towards NaBH4 in vitro, and may similarly decrease reactivity in naturally-occurring chlorophyll-protein complexes

    Developing young people's sense of self and place through sport

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    Previous research has recognized positive health implications, both physical and mental, as an outcome of participation in leisure pursuits. They provide opportunities for self-expression and stress reduction, as well as an environment in which people can socialize. Leisure activities, specifically sport activities, can play a significant role in young people's identity development. This paper explores the leisure activities in which young people in Adelaide, Australia participate. It examines the role of leisure activities in terms of young people's identity and feelings towards their hometown. This study consisted of semi-structured focus groups conducted with 24 senior high school students, followed by a survey resulting in 226 useable responses. Respondents were aged between 16 and 18 years of age. From the range of activities identified and explored, the results revealed sports activities to have the greatest impact on young people's lives. The results demonstrated that frequency of participation has a significant effect on young people's involvement levels and how they identify with the activity

    The Crystal Structure of CHIR-AB1: A Primordial Avian Classical Fc Receptor

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    CHIR-AB1 is a newly identified avian immunoglobulin (Ig) receptor that includes both activating and inhibitory motifs and was therefore classified as a potentially bifunctional receptor. Recently, CHIR-AB1 was shown to bind the Fc region of chicken IgY and to induce calcium mobilization via association with the common γ-chain, a subunit that transmits signals upon ligation of many different immunoreceptors. Here we describe the 1.8-Å-resolution crystal structure of the CHIR-AB1 ectodomain. The receptor ectodomain consists of a single C2-type Ig domain resembling the Ig-like domains found in mammalian Fc receptors such as FcγRs and FcαRI. Unlike these receptors and other monomeric Ig superfamily members, CHIR-AB1 crystallized as a 2-fold symmetrical homodimer that bears no resemblance to variable or constant region dimers in an antibody. Analytical ultracentrifugation demonstrated that CHIR-AB1 exists as a mixture of monomers and dimers in solution, and equilibrium gel filtration revealed a 2:1 receptor/ligand binding stoichiometry. Measurement of the 1:1 CHIR-AB1/IgY interaction affinity indicates a relatively low affinity complex, but a 2:1 CHIR-AB1/IgY interaction allows an increase in apparent affinity due to avidity effects when the receptor is tethered to a surface. Taken together, these results add to the structural understanding of Fc receptors and their functional mechanisms

    Spatial Heterogeneity of Seasonal Grazing Pressure Created by Herd Movement Patterns on Hilly Rangelands Using GPS and GIS

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    The spatial heterogeneity of grazing pressure on extensive rangelands has management implications (Adler et al., 2001) but it has traditionally been difficult to quantify. Combination of technologies based on GPS (Global Positioning System) and GIS (Geographic Information Systems) is a quantum leap in our ability to address this issue. These tools were used to estimate the spatial heterogeneity of grazing pressure at a farm scale, and examine the relation between local landscape features and local grazing pressure

    Localization of a 64-kDa phosphoprotein in the lumen between the outer and inner envelopes of pea chloroplasts

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    The identification and localization of a marker protein for the intermembrane space between the outer and inner chloroplast envelopes is described. This 64-kDa protein is very rapidly labeled by [γ-32P]ATP at very low (30 nM) ATP concentrations and the phosphoryl group exhibits a high turnover rate. It was possible to establish the presence of the 64-kDa protein in this plastid compartment by using different chloroplast envelope separation and isolation techniques. In addition comparison of labeling kinetics by intact and hypotonically lysed pea chloroplasts support the localization of the 64-kDa protein in the intermembrane space. The 64-kDa protein was present and could be labeled in mixed envelope membranes isolated from hypotonically lysed plastids. Mixed envelope membranes incorporated high amounts of 32P from [γ-32P]ATP into the 64-kDa protein, whereas separated outer and inner envelope membranes did not show significant phosphorylation of this protein. Water/Triton X-114 phase partitioning demonstrated that the 64-kDa protein is a hydrophilic polypeptide. These findings suggest that the 64-kDa protein is a soluble protein trapped in the space between the inner and outer envelope membranes. After sonication of mixed envelope membranes, the 64-kDa protein was no longer present in the membrane fraction, but could be found in the supernatant after a 110000 × g centrifugation

    Evaluating the boundary and covering degree of planar Minkowski sums and other geometrical convolutions

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    AbstractAlgorithms are developed, based on topological principles, to evaluate the boundary and “internal structure” of the Minkowski sum of two planar curves. A graph isotopic to the envelope curve is constructed by computing its characteristic points. The edges of this graph are in one-to-one correspondence with a set of monotone envelope segments. A simple formula allows a degree to be assigned to each face defined by the graph, indicating the number of times its points are covered by the Minkowski sum. The boundary can then be identified with the set of edges that separate faces of zero and non-zero degree, and the boundary segments corresponding to these edges can be approximated to any desired geometrical accuracy. For applications that require only the Minkowski sum boundary, the algorithm minimizes geometrical computations on the “internal” envelope edges, that do not contribute to the final boundary. In other applications, this internal structure is of interest, and the algorithm provides comprehensive information on the covering degree for different regions within the Minkowski sum. Extensions of the algorithm to the computation of Minkowski sums in R3, and other forms of geometrical convolution, are briefly discussed

    Expression Analysis of the Ligands for the Natural Killer Cell Receptors NKp30 and NKp44

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    BACKGROUND: The natural cytotoxicity receptors (NCR) are important to stimulate the activity of Natural Killer (NK) cells against transformed cells. Identification of NCR ligands and their level of expression on normal and neoplastic cells has important implications for the rational design of immunotherapy strategies for cancer. METHODOLOGY/PRINCIPAL FINDINGS: Here we analyze the expression of NKp30 ligand and NKp44 ligand on 30 transformed or non-transformed cell lines of different origin. We find intracellular and surface expression of these two ligands on almost all cell lines tested. Expression of NKp30 and NKp44 ligands was variable and did not correlate with the origin of the cell line. Expression of NKp30 and NKp44 ligand correlated with NKp30 and NKp44-mediated NK cell lysis of tumor cells, respectively. The surface expression of NKp30 ligand and NKp44 ligand was sensitive to trypsin treatment and was reduced in cells arrested in G(2)/M phase. CONCLUSION/SIGNIFICANCE: These data demonstrate the ubiquitous expression of the ligands for NKp30 and NKp44 and give an important insight into the regulation of these ligands
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