Complete genome sequence of Frog virus 3, isolated from a strawberry poison frog (Oophaga pumilio) imported from Nicaragua into the Netherlands

Frog virus 3 was isolated from a strawberry poison frog (Oophaga pumilio) imported from Nicaragua via Germany to the Netherlands, and its complete genome sequence was determined. Frog virus 3 isolate Op/2015/Netherlands/UU3150324001 is 107,183 bp long and has a nucleotide similarity of 98.26% to the reference Frog virus 3 isolate

Qué papel juega el hongo Phytophthora cinnamomi en el desarrollo de la regeneración natural y repoblaciones de encina y alcornoque

Se presentan los resultados resumidos de tres trabajos realizados para conocer el papel que juega el hongo Phytophthora cinnamomi en el desarrollo de plántulas de encina (Quercus ilex) y alcornoque (Quercus suber). Se realizaron dos prospecciones para detectar la presencia de P.cinnamomi en focos de seca en dehesas de encina y alcornoque de Extremadura en 1991-1992 y 1999-2000. El porcentaje de focos en los que se detectó la presencia de P. cinnamomi ha permanecido relativamente estable entre las dos prospecciones. También se determinó la tasa de crecimiento relativo para nueve aislados de P. cinnamomi a diferentes temperaturas, observando una elevada variabilidad tanto en la temperatura óptima de crecimiento como en el valor de la propia tasa diaria de crecimiento máxima. También se estudió la emergencia y supervivencia de plántulas de encina y alcornoque procedentes de dos dehesas ecológicamente diferentes, cultivadas en condiciones controladas en suelos de estas dehesas infectados naturalmente con P. cinnamomi y en estos mismos suelos previamente desinfectados en autoclave. Un factor limitante en los procesos de regeneración natural y en las repoblaciones por siembra directa de encinas y alcornoque puede ser su alta susceptibilidad a las infecciones por P. cinnamomi.Por último, se estudió durante dos años consecutivos, la supervivencia de las plantas de encina de una repoblación sobre un suelo naturalmente infectado con P. cinnamomi en la provincia de Badajoz (SO de España). Los resultados obtenidos se discuten teniendo en cuenta el déficit hídrico, los daños por P. cinnamomi, la competencia con otras especies y las posibles medidas sanitarias que amortigüen los daños en las repoblaciones con encinas._______________________________Three different studies were performed with the aim to determine the role of Phytophthora cinnamomi in the early development of seedlings of holm oak (Quercus ilex) and cork oak (Quercus suber). Two surveys, for the presence of Phytophthora cinnamomi in declining holm oak and cork oak stands in Extremadura (SW Spain) were performed in 1991-1992 and 1999-2000. The percentage of decline foci where P. cinnamomi is envolved has remained relatively stable in the lapse of time between the two surveys. Growth rates were determined for 9 P. cinnamomi isolates. Considerable variation in optimum growth temperatures and in daily growth rates occurred among isolates. The emergence and survival of pregerminated holm oak and cork oak acorns from two ecologically different dehesas (Mediterranean open woodlands) were studied in two soils from these stands naturally infected with P. cinnamomi, and in the same soils previously sterilized in the autoclave. The demonstrated high susceptibility of holm and cork oak young seedlings to P. cinnamomi could be a limiting factor in mediterranean opend woodlands (dehesas) not only in natural regenerationprocesses but also when reforestation by direct sowing is implemented. Finally, the viability of 1 year old holm oak seedlings in a soil naturally infected with P. cinnamomi was studied during two consecutive years in a plot located in SW Spain. Results obtained are discussed in the framework of seasonal water deficit, P. cinnamomi damage, weed competition and sanitation thechniques to be used in declined holm oak stands in Spain

Experimental study of solid mixing mechanism in a 2D fluidized bed

The main mechanism of solids mixing in bubbling fluidized beds is well understood. When a bubble rises through the bed, it carries a wake of particles to the bed surface. A downflow of solids exists in the region surrounding the rising bubbles, resulting on an overall convective circulation of particles in the axial direction (1). In this work, a new method to characterize solids mixing in a 2D fluidized bed is developed. This mixing index is able to macroscopically characterize the rate of mixing in a fluidized bed by means of DIA. The mixing index is analogous to the Lacey’s mixing index of particle mixing (2). The experiments are carried out in a pseudo-2D fluidized bed using glass beads as bed material. These glass beads have the same density and diameter but half of them are painted in black (Figure-1). At the beginning of each experiment, the particles are placed in a perfectly lateral segregated state and then the fluidizing air is suddenly injected while images are recorded. Two different regions are detected in the time evolution of the mixing index. The first one is a region with a fast convective mixing, where the initial boundary between the black and white particles is broken. The second one is a region where diffusive mixing is dominant and the particles clusters are mixed with the bulk following an exponential trend (Figure-1). These two mechanisms, as well as the overall mixing time are characterized for different superficial gas velocities and particle sizes. REFERENCES M.J. Rhodes, X.S. Wang, M. Nguyen, P. Stewart, K. Liffman. Study of mixing in gas-fluidized beds using DEM model. Chem. Eng. Sci., 56(8):2859-2866, 2001. P.M.C. Lacey. Developments in the theory of particle mixing. J. Appl. Chem., 4:257-268, 1954. Please click Additional Files below to see the full abstract

Prevalencia de anemia infecciosa equina en el Departamento de Córdoba.

Se hizo un estudio serológico con el objeto de determinar la prevalencia de anemia infecciosa equina en el Departamento de Córdoba, Colombia, y conocer la distribución y espidemiología en esta zona. Bajo condiciones prácticas de manejo y asepsia, se hizo la toma de muestras de sangre a 400 equinos elegidos completamente al azar, en 130 fincas distribuídas uniformemente en los 25 municipios que en la actualidad conforman el Departamento de Córdoba. Los sueros fueron analizados para determinar la presencia de anticuerpos precipitantes específicos para la anemia, utilizando para ello la prueba de inmunodifusión en agar-gel descrita por Coggins. De un total de 396 sueros estudiados, 54 resultaron con reacción positiva a anemia. Por no encontrarse diferencias significativas en cuanto a presentación de la enfermedad en el Alto, Medio y Bajo Sinú, se considera que la enfermedad se encuentra uniformemente distribuida en todo el Departamento. Se encontró que la enfermedad tiende a propagarse más fácilmente en fincas donde la población equina es más permanente, y por tanto donde existe una relación más estrecha entre animales, que en las que están sometidas a desplazamiento continuo de animales. Se comprueba una vez mas el bajo índice de diseminación con características de mortalidad de la enfermedad. No hubo relación entre la presentación de la enfermedad y la edad, sexo ni especie equin

Diagnostico de intemperismo en la catedral de puebla

This paper presents a physicochemical approach to diagnose basalt weathering at Cathedral of Puebla facade, building located at downtown Puebla, which was built in the XVII century and has the particularity of having three sides exposed to the action of rain, wind and sun. Applied methodology included physical inspection of the building, collection of weathered samples, which were grounded, mixed with distilled water to prepare an analytical sample, determined physicochemical parameters allowed to discriminate which anions are causing basalt weathering.

IgG anti-high density lipoprotein antibodies are elevated in abdominal aortic aneurysm and associated with lipid profile and clinical features

High-density lipoproteins cholesterol (HDLc) levels are decreased in abdominal aortic aneurysm (AAA), which is hallmarked by autoimmunity and lipid aortic deposits. To investigate whether IgG anti-HDL antibodies were present in AAA and their potential association with clinical features, IgG anti-HDL and total IgG along with HDLc plasma levels were measured in 488 AAA patients and 184 controls from the Viborg Vascular (VIVA) study, and in tissue-conditioned media from AAA intraluminal thrombus and media layer samples compared to control aortas. Higher IgG anti-HDL levels were found in AAA compared to controls, even after correcting for total IgG, and after adjusting for potential confounders. IgG anti-HDL levels were correlated with aortic diameter in univariate and adjusted multivariate analyses. IgG anti-HDL antibodies were negatively associated with HDLc levels before and after correcting for potential confounders. Increased anti-HDL antibodies were identified in tissue-conditioned media from AAA samples compared to healthy aortas, with higher levels being observed in the media layer. In conclusion, increased IgG anti-HDL levels (both in plasma and in tissue) are linked to AAA, associated with aortic diameter and HDLc levels. These data suggest a potential immune response against HDL in AAA and support an emerging role of anti-HDL antibodies in AAA

Solar Intranetwork Magnetic Elements: bipolar flux appearance

The current study aims to quantify characteristic features of bipolar flux appearance of solar intranetwork (IN) magnetic elements. To attack such a problem, we use the Narrow-band Filter Imager (NFI) magnetograms from the Solar Optical Telescope (SOT) on board \emph{Hinode}; these data are from quiet and an enhanced network areas. Cluster emergence of mixed polarities and IN ephemeral regions (ERs) are the most conspicuous forms of bipolar flux appearance within the network. Each of the clusters is characterized by a few well-developed ERs that are partially or fully co-aligned in magnetic axis orientation. On average, the sampled IN ERs have total maximum unsigned flux of several 10^{17} Mx, separation of 3-4 arcsec, and a lifetime of 10-15 minutes. The smallest IN ERs have a maximum unsigned flux of several 10^{16} Mx, separations less than 1 arcsec, and lifetimes as short as 5 minutes. Most IN ERs exhibit a rotation of their magnetic axis of more than 10 degrees during flux emergence. Peculiar flux appearance, e.g., bipole shrinkage followed by growth or the reverse, is not unusual. A few examples show repeated shrinkage-growth or growth-shrinkage, like magnetic floats in the dynamic photosphere. The observed bipolar behavior seems to carry rich information on magneto-convection in the sub-photospheric layer.Comment: 26 pages, 14 figure

Revista de Vertebrados de la Estación Biológica de Doñana

The Osteology of Barbus bocagei (Steindachner, 1866) (Pisces: Cyprinidae).El crecimiento del Cacho (Leuciscus cephalus pyraenaicus Günther, 1862) en el rio Jarama (cuenca del Tajo)Contribución al estudio de la bermejuela, Rutilus arcasi, Steindachner 1866 de la cuenca del Júcar (Osteichthyes: Cyprinidae) 1. MorfometríaDimensiones, estructura, forma y disposición en la cavidad de los nidos de Corvus monedula.Variaciones altitudinales en la composición de las comunidades nidificantes de aves de Sierra Nevada (Sur de España)Ecomorfología de una comunidadde Passeriformes en la Sierra de Cazorla, SE de EspañaLa Distribución del pez-sol (Lepolllis gibbostlS L.) en la Península IbéricaSobre la distribución de Barbus haasi (OSTARIOPHYSI: CYPRINIDAE)La Distribución de Barbus bocagei STEINDACHNER, 1865 (OSTARIOPHYSI: CYPRINIDAE) en la Península IbéricaLighy effects on circadian locomotor activity of Lacerta lepida under constant temperatureEvaluación de diferentes métodos para estimar el área de campeo de dos especies de iguánidos.Un Casal de Cartaxo-Nottenho (Saxicola rubetra) a criar em Portugal.Nidificación de Passer hispaniolensis en antiguos nidos de Dendrocopos major thanneri.Observación de un lince ibérico (Lynx pardina) en la provincia de Lugo. Norte de España.El Visón americano, Mustela vison SCHREBER, 1777 (MAMMALIA, MUSTELIDAE) en Cataluña, N.E. de la Península Ibérica.Peer reviewe

BB flavour tagging using charm decays at the LHCb experiment

An algorithm is described for tagging the flavour content at production of neutral $B$ mesons in the LHCb experiment. The algorithm exploits the correlation of the flavour of a $B$ meson with the charge of a reconstructed secondary charm hadron from the decay of the other $b$ hadron produced in the proton-proton collision. Charm hadron candidates are identified in a number of fully or partially reconstructed Cabibbo-favoured decay modes. The algorithm is calibrated on the self-tagged decay modes $B^+ \to J/\psi \, K^+$ and $B^0 \to J/\psi \, K^{*0}$ using $3.0\mathrm{\,fb}^{-1}$ of data collected by the LHCb experiment at $pp$ centre-of-mass energies of $7\mathrm{\,TeV}$ and $8\mathrm{\,TeV}$. Its tagging power on these samples of $B \to J/\psi \, X$ decays is $(0.30 \pm 0.01 \pm 0.01) \%$.Comment: All figures and tables, along with any supplementary material and additional information, are available at http://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-027.htm

Consenso colombiano de atención, diagnóstico y manejo de la infección por SARS-COV-2/COVID-19 en establecimientos de atención de la salud Recomendaciones basadas en consenso de expertos e informadas en la evidencia

The “Asociación Colombiana de Infectología” (ACIN) and the “Instituto de Evaluación de Nuevas Tecnologías de la Salud” (IETS) created a task force to develop recommendations for Covid 19 health care diagnosis, management and treatment informed, and based, on evidence. Theses reccomendations are addressed to the health personnel on the Colombian context of health services. © 2020 Asociacion Colombiana de Infectologia. All rights reserved