Reconstrucción digital de estructuras de tejados históricos: desarrollo de un flujo de trabajo de análisis altamente automatizado

[EN] Planning on adaptive reuse, maintenance and restoration of historic timber structuresrequiresextensive architectural and structural analysis of the actual condition. Current methods for a modellingof roof constructions consist of several manual steps including the time-consuming dimensional modelling. The continuous development of terrestrial laser scanners increases the accuracy, comfort and speed of the surveying work inroof constructions. Resultingpoint clouds enabledetailed visualisation of theconstructionsrepresented by single points or polygonal meshes, but in fact donot containinformation about the structural system and the beam elements. The developed workflow containsseveral processing steps on the point cloud dataset. The most important among them arethenormal vector computation, the segmentation of points to extract planarfaces, a classification of planarsegmentsto detect the beam side facesand finally theparametric modelling of the beams on the basis of classified segments. Thisenablesa highly automated transitionfrom raw point cloud data to a geometric model containing beams of the structural system. The geometric model,as well as additional information about the structural properties of involved wooden beams and their joints,is necessaryinput for a furtherstructural modellingof timber constructions. The results of the workflow confirm that the proposed methods work well for beams with a rectangularcross-section and minor deformations. Scan shadows and occlusionof beamsby additional installationsor interlockingbeamsdecreases the modelling performance, but in generala high level ofaccuracy and completeness isachieved ata high degree of automation.[ES] Las estructuras históricas de madera requieren un análisis arquitectónico y estructural exhaustivo de su condición real en aras de planificar la reutilización flexible, el mantenimiento y la restauración. Los métodos actuales que modelan las construcciones de cubiertas pasan por aplicar varias etapas en modo manual, que incluye el lento modelado dimensional. El desarrollo continuo de escáneres láser terrestres aumenta la exactitud, la comodidad y la velocidad del trabajo topográfico en construcciones de tejados. Las nubes de puntos resultantes permiten la visualización detallada de las construcciones representadas por puntos o mallas poligonales, pero de hecho no contienen información sobre el sistema estructural y los elementos del travesaño. El flujo de trabajo desarrollado contiene varias etapas de procesamiento en el conjunto de datos de la nube de puntos. Los más importantes son el cálculo del vector normal, la segmentación de puntos que extraen caras planas, la clasificación de segmentos planos que detectan las caras laterales del travesaño y, finalmente, el modelado paramétrico de los travesaños en función de los segmentos clasificados. Esto permite una transición altamente automatizada de los datos de la nube de puntos brutos a un modelo geométrico que contiene los travesaños del sistema estructural. El modelo geométrico, así como la información adicional sobre las propiedades estructurales de las vigas de madera involucradas y de sus juntas, es información necesaria de entrada para el modelado estructural eventual de las construcciones de madera. Los resultados del flujo de trabajo confirman que los métodos propuestos funcionan bien en travesaños que presentan secciones transversales rectangulares y deformaciones menores. Las sombras en los escaneados y las oclusiones de los travesaños a partir de instalaciones adicionales o vigas entrelazados disminuye el rendimiento del modelado, pero en general se logra un nivel de exactitud e integridad elevado con un alto grado de automatización.Pöchtrager, M.; Styhler-Aydın, G.; Döring-Williams, M.; Pfeifer, N. (2018). Digital reconstruction of historic roof structures: developing a workflow for a highly automated analysis. Virtual Archaeology Review. 9(19):21-33. doi:10.4995/var.2018.8855SWORD2133919Attene, M., & Spagnuolo, M. (2000). Automatic surface reconstruction from point sets in space. Computer Graphics Forum, 19(3), 457-465. doi:10.1111/1467-8659.00438Baik, A., Yaagoubi, R., & Boehm, J. (2015). 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vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development

Skip to Next Section The Drosophila gene vasa is required for pole plasm assembly and function, and also for completion of oogenesis. To investigate the role of vasa in oocyte development, we generated a new null mutation of vasa, which deletes the entire coding region. Analysis of vasa-null ovaries revealed that the gene is involved in the growth of germline cysts. In vasa-null ovaries, germaria are atrophied, and contain far fewer developing cysts than do wild-type germaria; a phenotype similar to, but less severe than, that of a null nanos allele. The null mutant also revealed roles for vasa in oocyte differentiation, anterior-posterior egg chamber patterning, and dorsal-ventral follicle patterning, in addition to its better-characterized functions in posterior embryonic patterning and pole cell specification. The anterior-posterior and dorsal-ventral patterning phenotypes resemble those observed in gurken mutants. vasa-null oocytes fail to efficiently accumulate many localized RNAs, such as Bicaudal-D, orb, oskar, and nanos, but still accumulate gurken RNA. However, GRK accumulation in the oocyte is severely reduced in the absence of vasa function, suggesting a function for VASA in activating gurken translation in wild-type ovaries

SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster

Sisters on the loose (SOLO) protein functions as a meiotic cohesin component critical for correct chromosome segregation in Drosophila

cDNA Cloning and Expression Analysis of Gustavus Gene in the Oriental River Prawn Macrobrachium nipponense

The gustavus gene is required for localizing pole plasm and specifying germ cells. Research on gustavus gene expression will advance our understanding of the biological function of gustavus in animals. A cDNA encoding gustavus protein was identified and termed MnGus in the oriental river prawn Macrobrachium nipponense. Bioinformatic analyses showed that this gene encoded a protein of 262 amino acids and the protein belongs to the Spsb1 family. Real-time quantitative PCR analyses revealed that the expression level of MnGus in prawn embryos was slightly higher at the cleavage stage than at the blastula stage, and reached the maximum level during the zoea stage of embryos. The minimum level of MnGus expression occurred during the perinucleolus stage in the ovary, while the maximum was at the oil globule stage, and then the level of MnGus expression gradually decreased with the advancement of ovarian development. The expression level of MnGus in muscle was much higher than that in other tissues in mature prawn. The gustavus cDNA sequence was firstly cloned from the oriental river prawn and the pattern of gene expression was described during oocyte maturation, embryonic development, and in other tissues. The differential expression patterns of MnGus in the embryo, ovary and other somatic tissues suggest that the gustavus gene performs multiple physiological functions in the oriental river prawn

The SPRY domain–containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation

Macrophages lacking SPSB2 have increased NO production and enhanced pathogen-killing capabilities due to decreased ubiquitin-mediated destruction of iNOS

The Meiotic Recombination Checkpoint Suppresses NHK-1 Kinase to Prevent Reorganisation of the Oocyte Nucleus in Drosophila

The meiotic recombination checkpoint is a signalling pathway that blocks meiotic progression when the repair of DNA breaks formed during recombination is delayed. In comparison to the signalling pathway itself, however, the molecular targets of the checkpoint that control meiotic progression are not well understood in metazoans. In Drosophila, activation of the meiotic checkpoint is known to prevent formation of the karyosome, a meiosis-specific organisation of chromosomes, but the molecular pathway by which this occurs remains to be identified. Here we show that the conserved kinase NHK-1 (Drosophila Vrk-1) is a crucial meiotic regulator controlled by the meiotic checkpoint. An nhk-1 mutation, whilst resulting in karyosome defects, does so independent of meiotic checkpoint activation. Rather, we find unrepaired DNA breaks formed during recombination suppress NHK-1 activity (inferred from the phosphorylation level of one of its substrates) through the meiotic checkpoint. Additionally DNA breaks induced by X-rays in cultured cells also suppress NHK-1 kinase activity. Unrepaired DNA breaks in oocytes also delay other NHK-1 dependent nuclear events, such as synaptonemal complex disassembly and condensin loading onto chromosomes. Therefore we propose that NHK-1 is a crucial regulator of meiosis and that the meiotic checkpoint suppresses NHK-1 activity to prevent oocyte nuclear reorganisation until DNA breaks are repaired

rasiRNA pathway controls antisense expression of Drosophila telomeric retrotransposons in the nucleus

Telomeres in Drosophila are maintained by the specialized telomeric retrotransposons HeT-A, TART and TAHRE. Sense transcripts of telomeric retroelements were shown to be the targets of a specialized RNA-interference mechanism, a repeat-associated short interfering (rasi)RNA-mediated system. Antisense rasiRNAs play a key role in this mechanism, highlighting the importance of antisense expression in retrotransposon silencing. Previously, bidirectional transcription was reported for the telomeric element TART. Here, we show that HeT-A is also bidirectionally transcribed, and HeT-A antisense transcription in ovaries is regulated by a promoter localized within its 3′ untranslated region. A remarkable feature of noncoding HeT-A antisense transcripts is the presence of multiple introns. We demonstrate that sense and antisense HeT-A-specific rasiRNAs are present in the same tissue, indicating that transcripts of both directions may be considered as natural targets of the rasiRNA pathway. We found that the expression of antisense transcripts of telomeric elements is regulated by the RNA silencing machinery, suggesting rasiRNA-mediated interplay between sense and antisense transcripts in the cell. Finally, this regulation occurs in the nucleus since disruption of the rasiRNA pathway leads to an accumulation of TART and HeT-A transcripts in germ cell nuclei

miRNA-Dependent Translational Repression in the Drosophila Ovary

Background: The Drosophila ovary is a tissue rich in post-transcriptional regulation of gene expression. Many of the regulatory factors are proteins identified via genetic screens. The more recent discovery of microRNAs, which in other animals and tissues appear to regulate translation of a large fraction of all mRNAs, raised the possibility that they too might act during oogenesis. However, there has been no direct demonstration of microRNA-dependent translational repression in the ovary. Methodology/Principal Findings: Here, quantitative analyses of transcript and protein levels of transgenes with or without synthetic miR-312 binding sites show that the binding sites do confer translational repression. This effect is dependent on the ability of the cells to produce microRNAs. By comparison with microRNA-dependent translational repression in other cell types, the regulated mRNAs and the protein factors that mediate repression were expected to be enriched in sponge bodies, subcellular structures with extensive similarities to the P bodies found in other cells. However, no such enrichment was observed. Conclusions/Significance: Our results reveal the variety of post-transcriptional regulatory mechanisms that operate in the Drosophila ovary, and have implications for the mechanisms of miRNA-dependent translational control used in the ovary.This work was supported in part by NIH grant GM54409 and in part by Research Grant No. 1-FY08-445. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Cellular and Molecular Biolog