Polynucleotide: Adenosine glycosidase is the sole activity of ribosome-inactivating proteins on DNA

Polynucleotide: adenosine glycosidases (PNAG) are a class of plant and bacterial enzymes commonly known as ribosome-inactivating proteins (RIP). They are presently classified as rRNA N-glycosidases in the enzyme nomenclature [EC 3.2.2.22]. Several activities on nucleic acids, other than depurination, have been attributed to PNAG: in particular modifications induced in circular plasmids, including linearisation and topological changes, and cleavage of guanidinic residues. Here we describe a chromatographic procedure to obtain nuclease-free PNAG by dye-chromatography onto Procion Red derivatized Sepharose®. Highly purified enzymes depurinate extensively pBR322 circular, supercoiled DNA at neutral pH and exhibit neither DNase nor DNA glycolyase activities, do not cause topological changes, and adenine is the only base released from DNA and rRNA, even at very high enzyme concentrations. A scanning force microscopy (SFM) study of pBR322 treated with saporin-S6 confirmed that (i) this PNAG binds extensively to the plasmid, (ii) the distribution of the bound saporin-S6 molecules along the DNA chain is markedly variable, (iii) plasmids already digested with saporin-S6 do not appear fragmented or topologically modified. The observations here described demonstrate that polynucleotide:adenosine glycosidase is the sole enzymatic activity of the four ribosome-inactivating proteins gelonin, momordin I, pokeweed antiviral protein from seeds and saporin-S6. These proteins belong to different families, suggesting that the findings here described may be generalized to all PNAG

A Main Sequence for Quasars

AD and MM-A acknowledge financial support from the Spanish Ministry for Economy and Competitiveness through grants AYA2013-42227-P and AYA2016-76682-C3-1-P. DD and CN acknowledge support from grants PAPIIT108716, UNAM, and CONACyT221398. EB and NB acknowledge grants 176003 Gravitation and the large scale structure of the Universe and 176001 Astrophysical spectroscopy of extragalactic objects supported by the Ministry of Education and Science of the Republic of Serbia

Massive ionized outflows in quasars

The most luminous quasars in the Hamburg- ESO (HE) survey show, at a high prevalence, CIV λ1549 and [OIII]λλ4959,5007 emission line profiles with high-amplitude blueshifts which indicate outflows occurring over a wide range of spatial scales. We found evidence in favor of the nuclear origin of the outflows diagnosed by [OIII]λλ 4959,5007. The derived ionized gas mass, kinetic power, and radiation thrust are extremely high, and suggest widespread feedback on the host galaxies of very luminous quasars, at cosmic epochs between 2 and 6 Gyr from the Big Bang

Quasar massive ionized outflows traced by CIV λ1549 and [OIII]λλ4959,5007

The most luminous quasars (with bolometric luminosities are ≳ 1047 erg/s) show a high prevalence of CIV λ1549 and [OIII]λλ4959,5007 emission line profiles with strong blueshifts. Blueshifts are interpreted as due to Doppler effect and selective obscuration, and indicate outflows occurring over a wide range of spatial scales. We found evidence in favor of the nuclear origin of the outflows diagnosed by [OIII]λλ4959,5007. The ionized gas mass, kinetic power, and mechanical thrust are extremely high, and suggest widespread feedback effects on the host galaxies of very luminous quasars, at cosmic epochs between 2 and 6 Gyr from the Big Bang. In this mini-review we summarize results obtained by our group and reported in several major papers in the last few years with an eye on challenging aspects of quantifying feedback effects in large samples of quasars

A Main Sequence For Quasars

The 4D eigenvector 1 parameter space defined by Sulentic et al. may be seen as a surrogate H-R diagram for quasars. As in the stellar H-R diagram, a source sequence can be easily identified. In the case of quasars, the main sequence appears to be mainly driven by Eddington ratio. A transition Eddington ratio may in part explain the striking observational differences between quasars at opposite ends of the main sequence. The eigenvector-1 approach opens the door towards properly contextualized models of quasar physics, geometry and kinematics. We review some of the progress that has been made over the past 15 years, and point out still unsolved issues

Highly accreting quasars: a tool for cosmology?

Highly accreting quasars are possible cosmological probes, as their Eddington ratio is expected to saturate toward values of order unity. We present preliminary estimates of redshift- independent source luminosities and the Hubble diagram for quasars in the redshift range 0.1 <~ z <~ 2.6

VizieR Online Data Catalog: Spectra of 28 intermediate redshift quasars (Sulentic+, 2017)

Spectroscopic data for 28 intermediate redshift quasars are identified in Table 1. Actual data files are in FITS format in the spectra sub-directory. Units are in wavelength in Angstroms, and specific flux in erg/s/cm2/Angstrom (pW/m3)x1E15 in the rest frame (i.e., after redshift correction). The last column of Table 1 reports the FITS file names. (2 data files)

Functional cortical source connectivity of resting state electroencephalographic alpha rhythms shows similar abnormalities in patients with mild cognitive impairment due to Alzheimer's and Parkinson's diseases

Objective: This study tested the hypothesis that markers of functional cortical source connectivity of resting state eyes-closed electroencephalographic (rsEEG) rhythms may be abnormal in subjects with mild cognitive impairment due to Alzheimer's (ADMCI) and Parkinson's (PDMCI) diseases compared to healthy elderly subjects (Nold). Methods: rsEEG data had been collected in ADMCI, PDMCI, and Nold subjects (N = 75 for any group). eLORETA freeware estimated functional lagged linear connectivity (LLC) from rsEEG cortical sources. Area under receiver operating characteristic (AUROC) curve indexed the accuracy in the classification of Nold and MCI individuals. Results: Posterior interhemispheric and widespread intrahemispheric alpha LLC solutions were abnormally lower in both MCI groups compared to the Nold group. At the individual level, AUROC curves of LLC solutions in posterior alpha sources exhibited moderate accuracies (0.70-0.72) in the discrimination of Nold vs. ADMCI-PDMCI individuals. No differences in the LLC solutions were found between the two MCI groups. Conclusions: These findings unveil similar abnormalities in functional cortical connectivity estimated in widespread alpha sources in ADMCI and PDMCI. This was true at both group and individual levels. Significance: The similar abnormality of alpha source connectivity in ADMCI and PDMCI subjects might reflect common cholinergic impairment. (C) 2018 International Federation of Clinical Neurophysiology. Published by Elsevier B.V. All rights reserved

Immunotoxins and Anticancer Drug Conjugate Assemblies: The Role of the Linkage between Components

Immunotoxins and antibody-drug conjugates are protein-based drugs combining a target-specific binding domain with a cytotoxic domain. Such compounds are potentially therapeutic against diseases including cancer, and several clinical trials have shown encouraging results. Although the targeted elimination of malignant cells is an elegant concept, there are numerous practical challenges that limit conjugates’ therapeutic use, including inefficient cellular uptake, low cytotoxicity, and off-target effects. During the preparation of immunoconjugates by chemical synthesis, the choice of the hinge component joining the two building blocks is of paramount importance: the conjugate must remain stable in vivo but must afford efficient release of the toxic moiety when the target is reached. Vast efforts have been made, and the present article reviews strategies employed in developing immunoconjugates, focusing on the evolution of chemical linkers