Reducing the Environmental Impacts of Garments through Industrially Scalable Closed-Loop Recycling: Life Cycle Assessment of a Recycled Wool Blend Sweater

Wool recycling has been practiced commercially for more than 200 years. This study used data from established, commercial processes with the aim of determining the environmental impacts of a recycled wool blend garment and the contribution of recycling to reducing impacts on the market for wool sweaters, in comparison to other emission reduction approaches relating to garment use. A cradle-to-grave life cycle assessment showed impacts of 0.05 kg CO2-e, 0.63 MJ, 0.58 L H2O-e and 0.95 L per wear of a recycled wool blend sweater for climate change, fossil energy demand, water stress and freshwater consumption, respectively. Impacts predominantly arose from garment manufacturing and consumer practices (retail and garment care). When a recycled wool blend sweater was maintained with best practice garment use and care, impacts were reduced by 66–90% relative to standard maintenance of a virgin pure wool sweater. Increasing the closed-loop recycling rate to 50% had the potential to reduce impacts for the wool sweater market 7–24%, depending on the impact category. Brands and consumers hold the key to increasing recycling rates and reducing environmental impacts via increased donation of garments for recycling and increased adoption of garments containing recycled wool

Sequencing of the Sea Lamprey (Petromyzon marinus) Genome Provides Insights into Vertebrate Evolution

Lampreys are representatives of an ancient vertebrate lineage that diverged from our own ∼500 million years ago. By virtue of this deeply shared ancestry, the sea lamprey (P. marinus) genome is uniquely poised to provide insight into the ancestry of vertebrate genomes and the underlying principles of vertebrate biology. Here, we present the first lamprey whole-genome sequence and assembly. We note challenges faced owing to its high content of repetitive elements and GC bases, as well as the absence of broad-scale sequence information from closely related species. Analyses of the assembly indicate that two whole-genome duplications likely occurred before the divergence of ancestral lamprey and gnathostome lineages. Moreover, the results help define key evolutionary events within vertebrate lineages, including the origin of myelin-associated proteins and the development of appendages. The lamprey genome provides an important resource for reconstructing vertebrate origins and the evolutionary events that have shaped the genomes of extant organisms

The Effect of SOD1 Mutation on Cellular Bioenergetic Profile and Viability in Response to Oxidative Stress and Influence of Mutation-Type.

Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the progressive degeneration of motor neurons. Substantial evidence implicates oxidative stress and mitochondrial dysfunction as early events in disease progression. Our aim was to ascertain whether mutation of the SOD1 protein increases metabolic functional susceptibility to oxidative stress. Here we used a motor neuron-like cell line (NSC34) stably transfected with various human mutant SOD1 transgenes (G93A, G37R, H48Q) to investigate the impact of oxidative stress on cell viability and metabolic function within intact cells. NSC34 cells expressing mutant SOD1 showed a dose dependent reduction in cell viability when exposed to oxidative stress induced by hydrogen peroxide, with variation between mutations. The G93A transfectants showed greater cell death and LDH release compared to cells transfected with the other SOD1 mutations, and H48Q showed an accelerated decline at later time points. Differences in mitochondrial bioenergetics, including mitochondrial respiration, coupling efficiency and proton leak, were identified between the mutations, consistent with the differences observed in viability. NSC34 cells expressing G93A SOD1 displayed reduced coupled respiration and mitochondrial membrane potential compared to controls. Furthermore, the G93A mutation had significantly increased metabolic susceptibility to oxidative stress, with hydrogen peroxide increasing ROS production, reducing both cellular oxygen consumption and glycolytic flux in the cell. This study highlights bioenergetic defects within a cellular model of ALS and suggests that oxidative stress is not only detrimental to oxygen consumption but also glycolytic flux, which could lead to an energy deficit in the cell

Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study

Background Huntington's disease is caused by a CAG repeat expansion in the huntingtin gene, HTT. Age at onset has been used as a quantitative phenotype in genetic analysis looking for Huntington's disease modifiers, but is hard to define and not always available. Therefore, we aimed to generate a novel measure of disease progression and to identify genetic markers associated with this progression measure. Methods We generated a progression score on the basis of principal component analysis of prospectively acquired longitudinal changes in motor, cognitive, and imaging measures in the 218 indivduals in the TRACK-HD cohort of Huntington's disease gene mutation carriers (data collected 2008–11). We generated a parallel progression score using data from 1773 previously genotyped participants from the European Huntington's Disease Network REGISTRY study of Huntington's disease mutation carriers (data collected 2003–13). We did a genome-wide association analyses in terms of progression for 216 TRACK-HD participants and 1773 REGISTRY participants, then a meta-analysis of these results was undertaken. Findings Longitudinal motor, cognitive, and imaging scores were correlated with each other in TRACK-HD participants, justifying use of a single, cross-domain measure of disease progression in both studies. The TRACK-HD and REGISTRY progression measures were correlated with each other (r=0·674), and with age at onset (TRACK-HD, r=0·315; REGISTRY, r=0·234). The meta-analysis of progression in TRACK-HD and REGISTRY gave a genome-wide significant signal (p=1·12 × 10−10) on chromosome 5 spanning three genes: MSH3, DHFR, and MTRNR2L2. The genes in this locus were associated with progression in TRACK-HD (MSH3 p=2·94 × 10−8 DHFR p=8·37 × 10−7 MTRNR2L2 p=2·15 × 10−9) and to a lesser extent in REGISTRY (MSH3 p=9·36 × 10−4 DHFR p=8·45 × 10−4 MTRNR2L2 p=1·20 × 10−3). The lead single nucleotide polymorphism (SNP) in TRACK-HD (rs557874766) was genome-wide significant in the meta-analysis (p=1·58 × 10−8), and encodes an aminoacid change (Pro67Ala) in MSH3. In TRACK-HD, each copy of the minor allele at this SNP was associated with a 0·4 units per year (95% CI 0·16–0·66) reduction in the rate of change of the Unified Huntington's Disease Rating Scale (UHDRS) Total Motor Score, and a reduction of 0·12 units per year (95% CI 0·06–0·18) in the rate of change of UHDRS Total Functional Capacity score. These associations remained significant after adjusting for age of onset. Interpretation The multidomain progression measure in TRACK-HD was associated with a functional variant that was genome-wide significant in our meta-analysis. The association in only 216 participants implies that the progression measure is a sensitive reflection of disease burden, that the effect size at this locus is large, or both. Knockout of Msh3 reduces somatic expansion in Huntington's disease mouse models, suggesting this mechanism as an area for future therapeutic investigation

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Using LCA and Circularity Indicators to Measure the Sustainability of Textiles—Examples of Renewable and Non-Renewable Fibres

Reducing environmental impacts by increasing circularity is highly relevant to the textiles sector. Here, we examine results from life cycle assessment (LCA) and circularity indicators applied to renewable and non-renewable fibres to evaluate the synergies between the two approaches for improving sustainability assessment of textiles. Using LCA, impacts were quantified for sweaters made from fossil feedstock-derived and bio-based PET. These same sweaters were scored using four circularity indicators. Both sweaters showed similar fossil energy footprints, but the bio-PET raw material acquisition stage greenhouse gas, water and land occupation impacts were 1.9 to 60 times higher, leading to higher full life cycle impacts. These contrasts were principally determined by what raw material acquisition processes were considered outside the system boundary of the alternative feedstocks. Using circularity indicators, fossil-feedstock PET scored lowest (worst) because the feedstock was from a non-renewable source. These examples highlight the limitations of LCA: the renewability or non-renewability of raw materials is not fully considered, and contrasts in processes included within system boundaries can preclude equitable comparisons. For LCA to be suitable for quantifying sustainability, it should be complemented by circularity indicators capable of demonstrating the contrast between renewable and non-renewable raw materials, particularly in the case of textiles

Clothing Lifespans: What Should Be Measuredand How

Increasing the use of each product, most often called longer lifespans, is an effective environmental strategy. This article discusses how garment lifespans can be described in order to be measured and compared. It answers two sub-questions: (1) what to measure (units), and (2) how to measure (methods). We introduce and define terms related to clothing lifespans and contribute to discussions about an appropriate functional unit for garments in life cycle assessments (LCA) and other environmental accounting tools. We use a global wardrobe survey to exemplify the units and methods. Clothing lifespans can be described and measured in years, the number of wears, cleaning cycles, and users. All have an independent value that show different and central aspects of clothing lifespans. A functional unit for LCAs should emphasise both the number of wears for all users as well as the service lifespan in years. Number of wears is the best measure for regular clothing, while number of years is most suited for occasion wear, because it is important to account for the need of more garments to cover all the relevant occasions during a specified time period. It is possible to study lifespan via carefully constructed surveys, providing key data relating to actual garment use

Using LCA and Circularity Indicators to Measure the Sustainability of Textiles—Examples of Renewable and Non-Renewable Fibres

Reducing environmental impacts by increasing circularity is highly relevant to the textiles sector. Here, we examine results from life cycle assessment (LCA) and circularity indicators applied to renewable and non-renewable fibres to evaluate the synergies between the two approaches for improving sustainability assessment of textiles. Using LCA, impacts were quantified for sweaters made from fossil feedstock-derived and bio-based PET. These same sweaters were scored using four circularity indicators. Both sweaters showed similar fossil energy footprints, but the bio-PET raw material acquisition stage greenhouse gas, water and land occupation impacts were 1.9 to 60 times higher, leading to higher full life cycle impacts. These contrasts were principally determined by what raw material acquisition processes were considered outside the system boundary of the alternative feedstocks. Using circularity indicators, fossil-feedstock PET scored lowest (worst) because the feedstock was from a non-renewable source. These examples highlight the limitations of LCA: the renewability or non-renewability of raw materials is not fully considered, and contrasts in processes included within system boundaries can preclude equitable comparisons. For LCA to be suitable for quantifying sustainability, it should be complemented by circularity indicators capable of demonstrating the contrast between renewable and non-renewable raw materials, particularly in the case of textiles