117 research outputs found
Lineage tree analysis of immunoglobulin variable-region gene mutations in autoimmune diseases: chronic activation, normal selection
Autoimmune diseases show high diversity in the affected organs, clinical manifestations and disease dynamics. Yet they all share common features, such as the ectopic germinal centers found in many affected tissues. Lineage trees depict the diversification, via somatic hypermutation (SHM), of immunoglobulin variable-region (IGV) genes. We previously developed an algorithm for quantifying the graphical properties of IGV gene lineage trees, allowing evaluation of the dynamical interplay between SHM and antigen-driven selection in different lymphoid tissues, species, and disease situations. Here, we apply this method to ectopic GC B cell clones from patients with Myasthenia Gravis, Rheumatoid Arthritis, and Sjögren’s Syndrome, using data scaling to minimize the effects of the large variability due to methodological differences between groups. Autoimmune trees were found to be significantly larger relative to normal controls. In contrast, comparison of the measurements for tree branching indicated that similar selection pressure operates on autoimmune and normal control clones
Biotransformation of anthracene and fluoranthene by Absidia fusca Linnemann
A strain of Absidia fusca was isolated from a pesticide-contaminated
soil (Annaba, Algeria). The biotransformation capability of this strain
towards two polycyclic aromatic hydrocarbons (PAHs): anthracene and
fluoranthene was compared to that exhibited by another strain of A.
fusca isolated from a non-contaminated milieu and considered as a
control. The results obtained were statistically analyzed and showed
that the strain isolated from the contaminated soil was more efficient
than the control to remove anthracene from the medium, during all the
kinetics (90% removed versus 45% after 24 hrs). Concerning
fluoranthene, the amount removed by both strains was very high during
the first 24 hrs however the control strain was slightly more efficient
(94% versus 89%) while the results were similar for the two strains
during the rest of the kinetics. This study reveals for the first time
the potential interest of the species A. fusca for the bioremediation
of PAHs
Spitzer Mapping of PAHs and H2 in Photodissociation Regions
The mid-infrared (MIR) spectra of dense photodissociation regions (PDRs) are
typically dominated by emission from polycyclic aromatic hydrocarbons (PAHs)
and the lowest pure rotational states of molecular hydrogen (H2); two species
which are probes of the physical properties of gas and dust in intense UV
radiation fields. We utilize the high angular resolution of the Infrared
Spectrograph on the Spitzer Space Telescope to construct spectral maps of the
PAH and H2 features for three of the best studied PDRs in the galaxy, NGC 7023,
NGC 2023 and IC 63. We present spatially resolved maps of the physical
properties, including the H2 ortho-to-para ratio, temperature, and G_o/n_H. We
also present evidence for PAH dehydrogenation, which may support theories of H2
formation on PAH surfaces, and a detection of preferential self-shielding of
ortho-H2. All PDRs studied exhibit average temperatures of ~500 - 800K, warm H2
column densities of ~10^20 cm^-2, G_o/n_H ~ 0.1 - 0.8, and ortho-to-para ratios
of ~ 1.8. We find that while the average of each of these properties is
consistent with previous single value measurements of these PDRs, when
available, the addition of spatial resolution yields a diversity of values with
gas temperatures as high as 1500 K, column densities spanning ~ 2 orders of
magnitude, and extreme ortho-to-para ratios of 3.Comment: 14 figure
Two rapid assays for screening of patulin biodegradation
Artículo sobre distintos ensayos para comprobar la biodegradación de la patulinaThe mycotoxin patulin is produced by the blue
mould pathogen Penicillium expansum in rotting apples
during postharvest storage. Patulin is toxic to a wide range
of organisms, including humans, animals, fungi and bacteria.
Wash water from apple packing and processing
houses often harbours patulin and fungal spores, which can
contaminate the environment. Ubiquitous epiphytic yeasts,
such as Rhodosporidium kratochvilovae strain LS11 which
is a biocontrol agent of P. expansum in apples, have the
capacity to resist the toxicity of patulin and to biodegrade
it. Two non-toxic products are formed. One is desoxypatulinic
acid. The aim of the work was to develop rapid,
high-throughput bioassays for monitoring patulin degradation
in multiple samples. Escherichia coli was highly
sensitive to patulin, but insensitive to desoxypatulinic acid.
This was utilized to develop a detection test for patulin,
replacing time-consuming thin layer chromatography or
high-performance liquid chromatography. Two assays for patulin degradation were developed, one in liquid medium
and the other in semi-solid medium. Both assays allow the
contemporary screening of a large number of samples. The
liquid medium assay utilizes 96-well microtiter plates and
was optimized for using a minimum of patulin. The semisolid
medium assay has the added advantage of slowing
down the biodegradation, which allows the study and isolation
of transient degradation products. The two assays are
complementary and have several areas of utilization, from
screening a bank of microorganisms for biodegradation
ability to the study of biodegradation pathways
WIYN Open Cluster Study. LXIII. Abundance in the Super-Metal-Rich Open Cluster NGC 6253 from Hydra Sprectroscopy of the 7774 Å Oxygen Triplet Region
We present a spectroscopic abundance analysis of the old, super-metal-rich open cluster NGC 6253, with emphasis on its O abundance. High-dispersion, 7774 Å O i triplet region spectra of 47 stars were obtained using Hydra II on the CTIO Blanco 4 m. Radial velocity analysis confirms 39 stars consistent with single star membership, primarily at the turnoff. Thirty-six of these are included in our abundance analysis. Our differential analysis relative to the Sun yields primarily scaled-solar values, with weighted cluster averages of [O/H] = +0.440 ± 0.020, [Fe/H] = +0.445 ± 0.014, [Al/H] = +0.487 ± 0.020, [Si/H] = +0.504 ± 0.018, and [Ni/H] = +0.702 ± 0.018 (where the errors are ). We discuss possible origins for the three known super-metal-rich clusters based upon their abundance patterns, Galactic locations, and space motions. The abundance patterns of NGC 6253 are very similar to those of NGC 6791 and NGC 6583. With the possible exception of oxygen, the abundances of these clusters are all close to scaled-solar, and they are similar to patterns seen in metal-rich disk dwarfs and giants. However, they also seem to differ from those of metal-rich bulge stars. We demonstrate that NGC 6253 is unusually oxygen rich (in [O/H]) for its 3.3 Gyr age. While we find [O/Fe] to be scaled-solar for NGC 6253, the more recently reported values for NGC 6791 show a large variation, from values close to scaled-solar down to values at least a factor of two below scaled-solar. We discuss the possibility that the scaled-solar [O/Fe] abundances of NGC 6253 and NGC 6791 might reflect a flattening of the Galactic [O/Fe] versus [Fe/H] relationship. This possibility may be consistent with disk star abundance data, which show an apparent "floor" at [O/Fe] for [Fe/H] , and with chemical evolution model results, which may predict such a flattening due to a decrease in supernova Fe yields at super-solar-metallicities. Orbit solutions for NGC 6791 allow that it may have formed in the inner disk and was then kicked out, but the origins of the other two much younger clusters remain mysterious. We re-evaluate the age of NGC 6583 in view of the evidence that the cluster is super-metal-rich, and confirm a probable age less than 1 Gyr (best range: 500–900 Myr). We also argue that it is unlikely the cluster is more than 3 kpc away (best range: 2–3 kpc) if the apparent turnoff, main sequence, and giants are all cluster members
Nuclear Disks of Gas and Dust in Early Type Galaxies and the Hunt for Massive Black Holes: Hubble Space Telescope Observations of NGC 6251
We discuss Hubble Space Telescope optical images and spectra of NGC 6251, a
giant E2 galaxy and powerful radio source at a distance of 106 Mpc (for H_0 =
70 km/s/Mpc). The galaxy is known to host a very well defined dust disk (O'Neil
et al. 1994); the exceptional resolution of our V and I images allows a
detailed study of the disk structure. Furthermore, narrow band images centered
on the Halpha+[NII] emission lines, reveal the presence of ionized gas in the
inner 0.3 arcsec of the disk. We used the HST/Faint Object Spectrograph with
the 0.09 arcsec aperture to study the velocity structure of the disk. Dynamical
models were constructed for two extreme (in terms of central concentration)
analytical representations of the stellar surface brightness profile, from
which the mass density and corresponding rotational velocity are derived
assuming a constant mass-to-light ratio (M/L)_V ~ 8.5 M_solar/L_solar. For both
representations of the stellar component, the models show that the gas is in
Keplerian motion around a central mass ~ 4 - 8 X 10^8 solar masses, and that
the contribution of radial flows to the velocity field is negligible.Comment: 45 pages, submitted to Ap
The Sun was not born in M 67
Using the most recent proper-motion determination of the old,
Solar-metallicity, Galactic open cluster M 67, in orbital computations in a
non-axisymmetric model of the Milky Way, including a bar and 3D spiral arms, we
explore the possibility that the Sun once belonged to this cluster. We have
performed Monte Carlo numerical simulations to generate the present-day orbital
conditions of the Sun and M 67, and all the parameters in the Galactic model.
We compute 3.5 \times 10^5 pairs of orbits Sun-M 67 looking for close
encounters in the past with a minimum distance approach within the tidal radius
of M 67. In these encounters we find that the relative velocity between the Sun
and M 67 is larger than 20 km/s. If the Sun had been ejected from M 67 with
this high velocity by means of a three-body encounter, this interaction would
destroy an initial circumstellar disk around the Sun, or disperse its already
formed planets. We also find a very low probability, much less than 10^-7, that
the Sun was ejected from M 67 by an encounter of this cluster with a giant
molecular cloud. This study also excludes the possibility that the Sun and M 67
were born in the same molecular cloud. Our dynamical results convincingly
demonstrate that M67 could not have been the birth cluster of our Solar System.Comment: Astronomical Journal accepted (35 pages, 9 figures
The X-ray fast-time variability of Sco X-2 (GX 349+2) with RXTE
Sco X-2 (GX 349+2) is a low-mass X-ray binary and Z source. We have analysed
156 ks of Rossi X-ray Timing Explorer data, obtained in 1998 January, on this
source. We investigated the fast-time variability as a function of position on
the Z track. During these observations, Sco X-2 traced out the most extensive Z
track ever reported from this object, making this the most comprehensive study
thus far. We found the broad peaked flaring branch noise that is typical of Sco
X-2, with a centroid frequency in the range 3.3--5.8 Hz. We also discovered low
frequency noise, and a new peaked noise feature, with centroid frequencies in
the range 5.4--7.6 Hz and 11--54 Hz, respectively. We discuss the phenomenology
of these features, their relationship with the power spectral components found
in other low-mass X-ray binaries, and the implications for current models. In
particular, the low frequency noise we observed was strongest at intermediate
energies, in contrast to the low frequency noise seen in other Z sources. We
also detected very low frequency noise, and have calculated complex cross
spectra between intensity and hardness. We found that the very low frequency
noise is not entirely due to motion along the Z track.Comment: 17 pages, 9 figures, minor improvements, accepted for publication in
MNRA
Inhibition of GPR30 by estriol prevents growth stimulation of triple-negative breast cancer cells by 17β-estradiol
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