Efecto biostimulante de Trichoderma atroviride en zapallo anco (Cucurbita moschata Duch. ex Poir)

The biostimulant effect of Trichoderma atroviride on seedling growth and commercial yield of pumpkin (Cucurbita moschata) has been assessed. The seedling stage was evaluated in a greenhouse and the treatments were T0: control -without inoculation-; T1: 200 ml.m-2 and T2: 500 ml.m-2 of T. atroviride SC 108 conidia. After 30 days, number of plants; root length; seedling height; number of sheets; aerial and root fresh and dry weight and root/aerial index were evaluated. Significant differences were found for seedling height in T1 compared to T0 and T2. The average air fresh weight was significantly higher in T1 compared to T2. The root fresh weight was statistically higher in T2 compared to the rest. Seedlings treated with higher doses of T. atroviride registered an increase in root mass, and below ground biomass indexes were closer to 1. The other variables did not register any statistical difference between treatments and T0. In the field, the treatments were as follows: T0: without inoculation; T2 and T3 inoculated with 20 ml.m-2 and 50 ml.m-2 in seedling stage respectively, and addition of 3 l.ha-1 of T. atroviride SC 108 conidia to the transplant. At 100, 108 and 115 days after the transplant (DPT), it was harvested. The number of plants that remained until the harvest and the fruits’ ammount and weight per plant were considered. T2 differed significantly, surpassing T0 and T1 in number of plants at harvest time. The amount of fruits did not show significant differences along the harvests. However, T2 showed greater weight of commercial fruits harvested at 108 DPT, providing greater precocity.Se evaluó el efecto bioestimulante de Trichoderma atroviride en el crecimiento de plantines y rendimiento comercial de zapallo (Cucurbita moschata). La etapa de plantin se evaluó en invernadero y los tratamientos fueron T0: control -sin inocular-; T1: 200 ml.m-2 y T2: 500 ml.m-2 de T. atroviride SC 108 conidios. A los 30 días se evaluó: número de plantas; longitud radical; altura del plantín; número de hojas; peso fresco y seco aéreo y radical e índice raíz/aéreo. Se halló diferencias significativas para altura de plantín en T1 respecto de T0 y T2. La media del peso fresco aéreo fue significativamente superior en T1 respecto a T2. El peso fresco radical fue superior estadísticamente en T2 respecto al resto. Plantines tratados con dosis más alta de T. atroviride registraron aumento en masa radical, e índices raíz/aéreo próximos a 1. El resto de las variables no registraron diferencias estadísticas entre tratamientos y T0. A campo, los tratamientos fueron T0: sin inocular; T2 y T3 inoculados con 20 ml.m-2 y 50 ml.m-2 en etapa de plantin respectivamente, y adición de 3 l.ha-1 de T. atroviride SC 108 conidios al trasplante. Se cosechó 100, 108 y 115 días posteriores al trasplante (DPT). Se evaluó cantidad de plantas que prosperaron a cosecha, número y peso de frutos por planta. El T2 se diferenció significativamente superando a T0 y T1 en número de plantas a cosecha. El número de frutos no mostró diferencias significativas en las diferentes cosechas. No obstante, T2 evidenció mayor peso de frutos comerciales cosechados a los 108 DPT, dando una mayor precocidad

Anti-Allergic Cromones Inhibit Histamine and Eicosanoid Release from Activated Human and Murine Mast Cells by Releasing Annexin A1

PMCID: PMC3601088This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Suitability Mapping of Solar Energy Potential of Selected Areas in Camarines Sur using ArcGIS

Abstract— Solar energy, the most common and scalable renewable energy, has a huge potential to supply the increasing electricity demand. Hence, proper site selection for deploying solar PV systems is required. This paper presents the development of a solar suitability map to identify potential sites for solar PV systems in the selected areas in the Rinconada District of the province of Camarines Sur, Philippines. ArcGIS analyzed the annual average solar radiation, weather datasets, and geographical conditions and performed suitability mapping combined with the Analytic Hierarchy Process (AHP) to identify the weights of the nine criteria of suitable site selection. Based on these factors, twenty-seven (27) barangays were found suitable, where the central and eastern parts of Barangay Malawag in Nabua and a massive part of Barangay Causip in Bula are the most suitable locations for large-scale solar PV installation

Absence of annexin I expression in B-cell non-Hodgkin's lymphomas and cell lines

BACKGROUND: Annexin I, one of the 20 members of the annexin family of calcium and phospholipid-binding proteins, has been implicated in diverse biological processes including signal transduction, mediation of apoptosis and immunosuppression. Previous studies have shown increased annexin I expression in pancreatic and breast cancers, while it is absent in prostate and esophageal cancers. RESULTS: Data presented here show that annexin I mRNA and protein are undetectable in 10 out of 12 B-cell lymphoma cell lines examined. Southern blot analysis indicates that the annexin I gene is intact in B-cell lymphoma cell lines. Aberrant methylation was examined as a cause for lack of annexin I expression by treating cells 5-Aza-2-deoxycytidine. Reexpression of annexin I was observed after prolonged treatment with the demethylating agent indicating methylation may be one of the mechanisms of annexin I silencing. Treatment of Raji and OMA-BL-1 cells with lipopolysaccharide, an inflammation inducer, and with hydrogen peroxide, a promoter of oxidative stress, also failed to induce annexin I expression. Annexin I expression was examined in primary lymphoma tissues by immunohistochemistry and presence of annexin I in a subset of normal B-cells and absence of annexin I expression in the lymphoma tissues were observed. These results show that annexin I is expressed in normal B-cells, and its expression is lost in all primary B-cell lymphomas and 10 of 12 B-cell lymphoma cell lines. CONCLUSIONS: Our results suggest that, similar to prostate and esophageal cancers, annexin I may be an endogenous suppressor of cancer development, and loss of annexin I may contribute to B-cell lymphoma development

Regulation of blood-brain barrier integrity by microbiome-associated methylamines and cognition by trimethylamine N-oxide.

BACKGROUND: Communication between the gut microbiota and the brain is primarily mediated via soluble microbe-derived metabolites, but the details of this pathway remain poorly defined. Methylamines produced by microbial metabolism of dietary choline and L-carnitine have received attention due to their proposed association with vascular disease, but their effects upon the cerebrovascular circulation have hitherto not been studied. RESULTS: Here, we use an integrated in vitro/in vivo approach to show that physiologically relevant concentrations of the dietary methylamine trimethylamine N-oxide (TMAO) enhanced blood-brain barrier (BBB) integrity and protected it from inflammatory insult, acting through the tight junction regulator annexin A1. In contrast, the TMAO precursor trimethylamine (TMA) impaired BBB function and disrupted tight junction integrity. Moreover, we show that long-term exposure to TMAO protects murine cognitive function from inflammatory challenge, acting to limit astrocyte and microglial reactivity in a brain region-specific manner. CONCLUSION: Our findings demonstrate the mechanisms through which microbiome-associated methylamines directly interact with the mammalian BBB, with consequences for cerebrovascular and cognitive function. Video abstract

Cancer-Associated Fibroblasts Neutralize the Anti-tumor Effect of CSF1 Receptor Blockade by Inducing PMN-MDSC Infiltration of Tumors.

Tumor-associated macrophages (TAM) contribute to all aspects of tumor progression. Use of CSF1R inhibitors to target TAM is therapeutically appealing, but has had very limited anti-tumor effects. Here, we have identified the mechanism that limited the effect of CSF1R targeted therapy. We demonstrated that carcinoma-associated fibroblasts (CAF) are major sources of chemokines that recruit granulocytes to tumors. CSF1 produced by tumor cells caused HDAC2-mediated downregulation of granulocyte-specific chemokine expression in CAF, which limited migration of these cells to tumors. Treatment with CSF1R inhibitors disrupted this crosstalk and triggered a profound increase in granulocyte recruitment to tumors. Combining CSF1R inhibitor with a CXCR2 antagonist blocked granulocyte infiltration of tumors and showed strong anti-tumor effects

Induction of immunosuppressive functions and NF-\u3baB by FLIP in monocytes

Immunosuppression is a hallmark of tumor progression, and treatments that inhibit or deplete monocytic myeloid-derived suppressive cells could promote anti-tumor immunity. c-FLIP is a central regulator of caspase-8-mediated apoptosis and necroptosis. Here we show that low-dose cytotoxic chemotherapy agents cause apoptosis linked to c-FLIP down-regulation selectively in monocytes. Enforced expression of c-FLIP or viral FLIP rescues monocytes from cytotoxicity and concurrently induces potent immunosuppressive activity, in T cell cultures and in vivo models of tumor progression and immunotherapy. FLIP-transduced human blood monocytes can suppress graft versus host disease. Neither expression of FLIP in granulocytes nor expression of other anti-apoptotic genes in monocytes conferred immunosuppression, suggesting that FLIP effects on immunosuppression are specific to monocytic lineage and distinct from death inhibition. Mechanistically, FLIP controls a broad transcriptional program, partially by NF-\u3baB activation. Therefore, modulation of FLIP in monocytes offers a means to elicit or block immunosuppressive myeloid cells

The Glucose Transporter 2 regulates CD8+ T cell function via environment sensing

T cell activation is associated with a profound and rapid metabolic response to meet increased energy demands for cell division, differentiation and development of effector function. Glucose uptake and engagement of the glycolytic pathway are major checkpoints for this event. Here we show that the low-affinity, concentration-dependent glucose transporter 2 (Glut2) regulates the development of CD8+ T cell effector responses in mice by promoting glucose uptake, glycolysis and glucose storage. Expression of Glut2 is modulated by environmental factors including glucose and oxygen availability and extracellular acidification. Glut2 is highly expressed by circulating, recently primed T cells, allowing efficient glucose uptake and storage. In glucose-deprived inflammatory environments, Glut2 becomes downregulated, thus preventing passive loss of intracellular glucose. Mechanistically, Glut2 expression is regulated by a combination of molecular interactions involving hypoxia-inducible factor-1 alpha, galectin-9 and stomatin. Finally, we show that human T cells also rely on this glucose transporter, thus providing a potential target for therapeutic immunomodulation

Expressed sequence tag analysis of adult human optic nerve for NEIBank: Identification of cell type and tissue markers

<p>Abstract</p> <p>Background</p> <p>The optic nerve is a pure white matter central nervous system (CNS) tract with an isolated blood supply, and is widely used in physiological studies of white matter response to various insults. We examined the gene expression profile of human optic nerve (ON) and, through the NEIBANK online resource, to provide a resource of sequenced verified cDNA clones. An un-normalized cDNA library was constructed from pooled human ON tissues and was used in expressed sequence tag (EST) analysis. Location of an abundant oligodendrocyte marker was examined by immunofluorescence. Quantitative real time polymerase chain reaction (qRT-PCR) and Western analysis were used to compare levels of expression for key calcium channel protein genes and protein product in primate and rodent ON.</p> <p>Results</p> <p>Our analyses revealed a profile similar in many respects to other white matter related tissues, but significantly different from previously available ON cDNA libraries. The previous libraries were found to include specific markers for other eye tissues, suggesting contamination. Immune/inflammatory markers were abundant in the new ON library. The oligodendrocyte marker QKI was abundant at the EST level. Immunofluorescence revealed that this protein is a useful oligodendrocyte cell-type marker in rodent and primate ONs. L-type calcium channel EST abundance was found to be particularly low. A qRT-PCR-based comparative mammalian species analysis reveals that L-type calcium channel expression levels are significantly lower in primate than in rodent ON, which may help account for the class-specific difference in responsiveness to calcium channel blocking agents. Several known eye disease genes are abundantly expressed in ON. Many genes associated with normal axonal function, mRNAs associated with axonal transport, inflammation and neuroprotection are observed.</p> <p>Conclusion</p> <p>We conclude that the new cDNA library is a faithful representation of human ON and EST data provide an initial overview of gene expression patterns in this tissue. The data provide clues for tissue-specific and species-specific properties of human ON that will help in design of therapeutic models.</p