3,187 research outputs found
Whole Exome Sequencing of Cell-Free DNA for Early Lung Cancer: A Pilot Study to Differentiate Benign From Malignant CT-Detected Pulmonary Lesions
Introduction: Indeterminate pulmonary lesions (IPL) detected by CT pose a significant clinical challenge, frequently necessitating long-term surveillance or biopsy for diagnosis. In this pilot investigation, we performed whole exome sequencing (WES) of plasma cell free (cfDNA) and matched germline DNA in patients with CT-detected pulmonary lesions to determine the feasibility of somatic cfDNA mutations to differentiate benign from malignant pulmonary nodules.Methods: 33 patients with a CT-detected pulmonary lesions were retrospectively enrolled (n = 16 with a benign nodule, n = 17 with a malignant nodule). Following isolation and amplification of plasma cfDNA and matched peripheral blood mononuclear cells (PBMC) from patient blood samples, WES of cfDNA and PBMC DNA was performed. After genomic alignment and filtering, we looked for lung-cancer associated driver mutations and next identified high-confidence somatic variants in both groups.Results: Somatic cfDNA mutations were observed in both groups, with the cancer group demonstrating more variants than the benign group (1083 ± 476 versus 553 ± 519, p < 0.0046). By selecting variants present in >2 cancer patients and not the benign group, we accurately identified 82% (14/17) of cancer patients.Conclusions: This study suggests a potential role for cfDNA for the early identification of lung cancer in patients with CT-detected pulmonary lesions. Importantly, a substantial number of somatic variants in healthy patients with benign pulmonary nodules were also found. Such “benign” variants, while largely unexplored to date, have widespread relevance to all liquid biopsies if cfDNA is to be used accurately for cancer detection
A Bright Submillimeter Source in the Bullet Cluster (1E0657--56) Field Detected with BLAST
We present the 250, 350, and 500 micron detection of bright submillimeter
emission in the direction of the Bullet Cluster measured by the Balloon-borne
Large Aperture Submillimeter Telescope (BLAST). The 500 micron centroid is
coincident with an AzTEC 1.1 mm point-source detection at a position close to
the peak lensing magnification produced by the cluster. However, the 250 micron
and 350 micron centroids are elongated and shifted toward the south with a
differential shift between bands that cannot be explained by pointing
uncertainties. We therefore conclude that the BLAST detection is likely
contaminated by emission from foreground galaxies associated with the Bullet
Cluster. The submillimeter redshift estimate based on 250-1100 micron
photometry at the position of the AzTEC source is z_phot = 2.9 (+0.6 -0.3),
consistent with the infrared color redshift estimation of the most likely IRAC
counterpart. These flux densities indicate an apparent far-infrared luminosity
of L_FIR = 2E13 Lsun. When the amplification due to the gravitational lensing
of the cluster is removed, the intrinsic far-infrared luminosity of the source
is found to be L_FIR <= 10^12 Lsun, consistent with typical luminous infrared
galaxies.Comment: Accepted for publication in the Astrophysical Journal. Maps are
available at http://blastexperiment.info
The BLAST View of the Star Forming Region in Aquila (ell=45deg,b=0deg)
We have carried out the first general submillimeter analysis of the field
towards GRSMC 45.46+0.05, a massive star forming region in Aquila. The
deconvolved 6 deg^2 (3\degree X 2\degree) maps provided by BLAST in 2005 at
250, 350, and 500 micron were used to perform a preliminary characterization of
the clump population previously investigated in the infrared, radio, and
molecular maps. Interferometric CORNISH data at 4.8 GHz have also been used to
characterize the Ultracompact HII regions (UCHIIRs) within the main clumps. By
means of the BLAST maps we have produced an initial census of the submillimeter
structures that will be observed by Herschel, several of which are known
Infrared Dark Clouds (IRDCs). Our spectral energy distributions of the main
clumps in the field, located at ~7 kpc, reveal an active population with
temperatures of T~35-40 K and masses of ~10^3 Msun for a dust emissivity index
beta=1.5. The clump evolutionary stages range from evolved sources, with
extended HII regions and prominent IR stellar population, to massive young
stellar objects, prior to the formation of an UCHIIR.The CORNISH data have
revealed the details of the stellar content and structure of the UCHIIRs. In
most cases, the ionizing stars corresponding to the brightest radio detections
are capable of accounting for the clump bolometric luminosity, in most cases
powered by embedded OB stellar clusters
Substance use disorders and risk of suicide in a general US population: a case control study
BACKGROUND: Prior research suggests that substance use disorders (SUDs) are associated with risk of suicide mortality, but most previous work has been conducted among Veterans Health Administration patients. Few studies have examined the relationship between SUDs and suicide mortality in general populations. Our study estimates the association of SUDs with suicide mortality in a general US population of men and women who receive care across eight integrated health systems.
METHODS: We conducted a case-control study using electronic health records and claims data from eight integrated health systems of the Mental Health Research Network. Participants were 2674 men and women who died by suicide between 2000-2013 and 267,400 matched controls. The main outcome was suicide mortality, assessed using data from the health systems and confirmed by state death data systems. Demographic and diagnostic data on substance use disorders and other health conditions were obtained from each health system. First, we compared descriptive statistics for cases and controls, including age, gender, income, and education. Next, we compared the rate of each substance use disorder category for cases and controls. Finally, we used conditional logistic regression models to estimate unadjusted and adjusted odds of suicide associated with each substance use disorder category.
RESULTS: All categories of substance use disorders were associated with increased risk of suicide mortality. Adjusted odds ratios ranged from 2.0 (CI 1.7, 2.3) for patients with tobacco use disorder only to 11.2 (CI 8.0, 15.6) for patients with multiple alcohol, drug, and tobacco use disorders. Substance use disorders were associated with increased relative risk of suicide for both women and men across all categories, but the relative risk was more pronounced in women.
CONCLUSIONS: Substance use disorders are associated with significant risk of suicide mortality, especially for women, even after controlling for other important risk factors. Experiencing multiple substance use disorders is particularly risky. These findings suggest increased suicide risk screening and prevention efforts for individuals with substance use disorders are needed
BLAST05: Power Spectra of Bright Galactic Cirrus at Submillimeter Wavelengths
We report multi-wavelength power spectra of diffuse Galactic dust emission
from BLAST observations at 250, 350, and 500 microns in Galactic Plane fields
in Cygnus X and Aquila. These submillimeter power spectra statistically
quantify the self-similar structure observable over a broad range of scales and
can be used to assess the cirrus noise which limits the detection of faint
point sources. The advent of submillimeter surveys with the Herschel Space
Observatory makes the wavelength dependence a matter of interest. We show that
the observed relative amplitudes of the power spectra can be related through a
spectral energy distribution (SED). Fitting a simple modified black body to
this SED, we find the dust temperature in Cygnus X to be 19.9 +/- 1.3 K and in
the Aquila region 16.9 +/- 0.7 K. Our empirical estimates provide important new
insight into the substantial cirrus noise that will be encountered in
forthcoming observations.Comment: Submitted to the Astrophysical Journal. Maps and other data are
available at http://blastexperiment.info
Submillimeter Number Counts From Statistical Analysis of BLAST Maps
We describe the application of a statistical method to estimate submillimeter
galaxy number counts from confusion limited observations by the Balloon-borne
Large Aperture Submillimeter Telescope (BLAST). Our method is based on a
maximum likelihood fit to the pixel histogram, sometimes called 'P(D)', an
approach which has been used before to probe faint counts, the difference being
that here we advocate its use even for sources with relatively high
signal-to-noise ratios. This method has an advantage over standard techniques
of source extraction in providing an unbiased estimate of the counts from the
bright end down to flux densities well below the confusion limit. We
specifically analyse BLAST observations of a roughly 10 sq. deg. map centered
on the Great Observatories Origins Deep Survey South (GOODS-S) field. We
provide estimates of number counts at the three BLAST wavelengths, 250, 350,
and 500 microns; instead of counting sources in flux bins we estimate the
counts at several flux density nodes connected with power-laws. We observe a
generally very steep slope for the counts of about -3.7 at 250 microns and -4.5
at 350 and 500 microns, over the range ~0.02-0.5 Jy, breaking to a shallower
slope below about 0.015 Jy at all three wavelengths. We also describe how to
estimate the uncertainties and correlations in this method so that the results
can be used for model-fitting. This method should be well-suited for analysis
of data from the Herschel satellite.Comment: Accepted for publication in the Astrophysical Journal; see associated
data and other papers at http://blastexperiment.info
The BLAST Survey of the Vela Molecular Cloud: Physical Properties of the Dense Cores in Vela-D
The Balloon-borne Large-Aperture Submillimeter Telescope (BLAST) carried out
a 250, 350 and 500 micron survey of the galactic plane encompassing the Vela
Molecular Ridge, with the primary goal of identifying the coldest dense cores
possibly associated with the earliest stages of star formation. Here we present
the results from observations of the Vela-D region, covering about 4 square
degrees, in which we find 141 BLAST cores. We exploit existing data taken with
the Spitzer MIPS, IRAC and SEST-SIMBA instruments to constrain their
(single-temperature) spectral energy distributions, assuming a dust emissivity
index beta = 2.0. This combination of data allows us to determine the
temperature, luminosity and mass of each BLAST core, and also enables us to
separate starless from proto-stellar sources. We also analyze the effects that
the uncertainties on the derived physical parameters of the individual sources
have on the overall physical properties of starless and proto-stellar cores,
and we find that there appear to be a smooth transition from the pre- to the
proto-stellar phase. In particular, for proto-stellar cores we find a
correlation between the MIPS24 flux, associated with the central protostar, and
the temperature of the dust envelope. We also find that the core mass function
of the Vela-D cores has a slope consistent with other similar (sub)millimeter
surveys.Comment: Accepted for publication in the Astrophysical Journal. Data and maps
are available at http://blastexperiment.info
Deconvolution of Images from BLAST 2005: Insight into the K3-50 and IC 5146 Star-Forming Regions
We present an implementation of the iterative flux-conserving Lucy-Richardson
(L-R) deconvolution method of image restoration for maps produced by the
Balloon-borne Large Aperture Submillimeter Telescope (BLAST). We have analyzed
its performance and convergence extensively through simulations and
cross-correlations of the deconvolved images with available highresolution
maps. We present new science results from two BLAST surveys, in the Galactic
regions K3-50 and IC 5146, further demonstrating the benefits of performing
this deconvolution.
We have resolved three clumps within a radius of 4.'5 inside the star-forming
molecular cloud containing K3-50. Combining the well-resolved dust emission map
with available multi-wavelength data, we have constrained the Spectral Energy
Distributions (SEDs) of five clumps to obtain masses (M), bolometric
luminosities (L), and dust temperatures (T). The L-M diagram has been used as a
diagnostic tool to estimate the evolutionary stages of the clumps. There are
close relationships between dust continuum emission and both 21-cm radio
continuum and 12CO molecular line emission.
The restored extended large scale structures in the Northern Streamer of IC
5146 have a strong spatial correlation with both SCUBA and high resolution
extinction images. A dust temperature of 12 K has been obtained for the central
filament. We report physical properties of ten compact sources, including six
associated protostars, by fitting SEDs to multi-wavelength data. All of these
compact sources are still quite cold (typical temperature below ~ 16 K) and are
above the critical Bonner-Ebert mass. They have associated low-power Young
Stellar Objects (YSOs). Further evidence for starless clumps has also been
found in the IC 5146 region.Comment: 13 pages, 12 Figures, 3 Table
The Balloon-Borne Large Aperture Submillimeter Telescope (BLAST) 2005: A 10 deg^2 Survey of Star Formation in Cygnus X
We present Cygnus X in a new multi-wavelength perspective based on an
unbiased BLAST survey at 250, 350, and 500 micron, combined with rich datasets
for this well-studied region. Our primary goal is to investigate the early
stages of high mass star formation. We have detected 184 compact sources in
various stages of evolution across all three BLAST bands. From their
well-constrained spectral energy distributions, we obtain the physical
properties mass, surface density, bolometric luminosity, and dust temperature.
Some of the bright sources reaching 40 K contain well-known compact H II
regions. We relate these to other sources at earlier stages of evolution via
the energetics as deduced from their position in the luminosity-mass (L-M)
diagram. The BLAST spectral coverage, near the peak of the spectral energy
distribution of the dust, reveals fainter sources too cool (~ 10 K) to be seen
by earlier shorter-wavelength surveys like IRAS. We detect thermal emission
from infrared dark clouds and investigate the phenomenon of cold ``starless
cores" more generally. Spitzer images of these cold sources often show stellar
nurseries, but these potential sites for massive star formation are ``starless"
in the sense that to date there is no massive protostar in a vigorous accretion
phase. We discuss evolution in the context of the L-M diagram. Theory raises
some interesting possibilities: some cold massive compact sources might never
form a cluster containing massive stars; and clusters with massive stars might
not have an identifiable compact cold massive precursor.Comment: 42 pages, 31 Figures, 6 table
The Impossibility of a Perfectly Competitive Labor Market
Using the institutional theory of transaction cost, I demonstrate that the assumptions of the competitive labor market model are internally contradictory and lead to the conclusion that on purely theoretical grounds a perfectly competitive labor market is a logical impossibility. By extension, the familiar diagram of wage determination by supply and demand is also a logical impossibility and the neoclassical labor demand curve is not a well-defined construct. The reason is that the perfectly competitive market model presumes zero transaction cost and with zero transaction cost all labor is hired as independent contractors, implying multi-person firms, the employment relationship, and labor market disappear. With positive transaction cost, on the other hand, employment contracts are incomplete and the labor supply curve to the firm is upward sloping, again causing the labor demand curve to be ill-defined. As a result, theory suggests that wage rates are always and everywhere an amalgam of an administered and bargained price. Working Paper 06-0
- …