Global and regional IUCN Red List Assessments: 1

In this contribution, the conservation status assessment of six plant species according to IUCN categories and criteria are presented. It includes the assessment at global level of Linaria tonzigii Lona, Allium garganicum Brullo, Pavone, Salmeri & Terrasi, Ferula arrigonii Bocchieri, Orchis patens Desf. subsp. patens and Armeria saviana Selvi and the assessment at regional level (Italy) of Viola jordanii Hanry

Multilocus genotyping reveals new molecular markers for differentiating distinct genetic lineages among “candidatus phytoplasma solani” strains associated with grapevine bois noir

Grapevine Bois noir (BN) is associated with infection by “Candidatus Phytoplasma solani” (CaPsol). In this study, an array of CaPsol strains was identified from 142 symptomatic grapevines in vineyards of northern, central, and southern Italy and North Macedonia. Molecular typing of the CaPsol strains was carried out by analysis of genes encoding 16S rRNA and translation elongation factor EF-Tu, as well as eight other previously uncharacterized genomic fragments. Strains of tuf-type a and b were found to be differentially distributed in the examined geographic regions in correlation with the prevalence of nettle and bindweed. Two sequence variants were identified in each of the four genomic segments harboring hlyC, cbiQ-glyA, trxA-truB-rsuA, and rplS-tyrS-csdB, respectively. Fifteen CaPsol lineages were identified based on distinct combinations of sequence variations within these genetic loci. Each CaPsol lineage exhibited a unique collective restriction fragment length polymorphism (RFLP) pattern and differed from each other in geographic distribution, probably in relation to the diverse ecological complexity of vineyards and their surroundings. This RFLP-based typing method could be a useful tool for investigating the ecology of CaPsol and the epidemiology of its associated diseases. Phylogenetic analyses highlighted that the sequence variants of the gene hlyC, which encodes a hemolysin III-like protein, separated into two clusters consistent with the separation of two distinct lineages on the basis of tufB gene sequences. Alignments of deduced full protein sequences of elongation factor-Tu (tufB gene) and hemolysin III-like protein (hlyC gene) revealed the presence of critical amino acid substitutions distinguishing CaPsol strains of tuf-type a and b. Findings from the present study provide new insights into the genetic diversity and ecology of CaPsol populations in vineyards

Notulae to the Italian native vascular flora: 9

In this contribution, new data concerning the distribution of native vascular flora in Italy are presented. It includes new records, confirmations, exclusions, and status changes to the Italian administrative regions. Two new combinations are proposed. Nomenclatural and distribution updates, published elsewhere, and corrigenda are provided as Suppl. material

Notulae to the Italian native vascular flora: 9

In this contribution, new data concerning the distribution of native vascular flora in Italy are presented. It includes new records, confirmations, exclusions, and status changes to the Italian administrative regions. Two new combinations are proposed. Nomenclatural and distribution updates, published elsewhere, and corrigenda are provided as Suppl. material 1

Folding of Matrix Metalloproteinase-2 Prevents Endogenous Generation of MHC Class-I Restricted Epitope

BACKGROUND: We previously demonstrated that the matrix metalloproteinase-2 (MMP-2) contained an antigenic peptide recognized by a CD8 T cell clone in the HLA-A*0201 context. The presentation of this peptide on class I molecules by human melanoma cells required a cross-presentation mechanism. Surprisingly, the classical endogenous processing pathway did not process this MMP-2 epitope. METHODOLOGY/PRINCIPAL FINDINGS: By PCR directed mutagenesis we showed that disruption of a single disulfide bond induced MMP-2 epitope presentation. By Pulse-Chase experiment, we demonstrated that disulfide bonds stabilized MMP-2 and impeded its degradation. Finally, using drugs, we documented that mutated MMP-2 epitope presentation used the proteasome and retrotranslocation complex. CONCLUSIONS/SIGNIFICANCE: These data appear crucial to us since they established the existence of a new inhibitory mechanism for the generation of a T cell epitope. In spite of MMP-2 classified as a self-antigen, the fact that cross-presentation is the only way to present this MMP-2 epitope underlines the importance to target this type of antigen in immunotherapy protocols

Tumor-specific expression of αvβ3 integrin promotes spontaneous metastasis of breast cancer to bone

INTRODUCTION: Studies in xenograft models and experimental models of metastasis have implicated several β3 integrin-expressing cell populations, including endothelium, platelets and osteoclasts, in breast tumor progression. Since orthotopic human xenograft models of breast cancer are poorly metastatic to bone and experimental models bypass the formation of a primary tumor, however, the precise contribution of tumor-specific αvβ3 to the spontaneous metastasis of breast tumors from the mammary gland to bone remains unclear. METHODS: We used a syngeneic orthotopic model of spontaneous breast cancer metastasis to test whether exogenous expression of αvβ3 in a mammary carcinoma line (66cl4) that metastasizes to the lung, but not to bone, was sufficient to promote its spontaneous metastasis to bone from the mammary gland. The tumor burden in the spine and the lung following inoculation of αvβ3-expressing 66cl4 (66cl4beta3) tumor cells or control 66cl4pBabe into the mammary gland was analyzed by real-time quantitative PCR. The ability of these cells to grow and form osteolytic lesions in bone was determined by histology and tartrate-resistant acid phosphatase staining of bone sections following intratibial injection of tumor cells. The adhesive, migratory and invasive properties of 66cl4pBabe and 66cl4beta3 cells were evaluated in standard in vitro assays. RESULTS: The 66cl4beta3 tumors showed a 20-fold increase in metastatic burden in the spine compared with 66cl4pBabe. A similar trend in lung metastasis was observed. αvβ3 did not increase the proliferation of 66cl4 cells in vitro or in the mammary gland in vivo. Similarly, αvβ3 is not required for the proliferation of 66cl4 cells in bone as both 66cl4pBabe and 66cl4beta3 proliferated to the same extent when injected directly into the tibia. 66cl4beta3 tumor growth in the tibia, however, increased osteoclast recruitment and bone resorption compared with 66cl4 tumors. Moreover, αvβ3 increased 66cl4 tumor cell adhesion and αvβ3-dependent haptotactic migration towards bone matrix proteins, as well as their chemotactic response to bone-derived soluble factors in vitro. CONCLUSION: These results demonstrate for the first time that tumor-specific αvβ3 contributes to spontaneous metastasis of breast tumors to bone and suggest a critical role for this receptor in mediating chemotactic and haptotactic migration towards bone factors

Carex phyllostachys C. A. MEY. (Cyperaceae) new for Italy and Phytogeographical Considerations

The occurrence of Carex phyllostachys C. A. MEY. has been reported from Santeramo in Colle (Apulia, southeastern Italy) in a deciduous wood, ascribable to the association Stipo bromoidis-Quercetum dalechampii. It is the first record of this species in Italy. The new site represents the westernmost limit of the distribution of this mostly Caucasian species. In Europe the species is very rare, until now known from the Balkan Peninsula only. The occurrence of this species in Apulia underlines once more the affinities between the flora and the vegetation of this Italian region and southeastern Europe. The IUCN assessment leads to consider the species as Critically Endangered (CR) in Italy

Notulae: 2135–2136. Gagea lacaitae (Liliaceae), Ornithogalum etruscum subsp. etruscum (Asparagaceae). In: Bartolucci F., Nepi C., Domina G., Peruzzi L. (eds.), Notulae alla checklist della flora vascolare italiana: 20

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Ophioglossum lusitanicum L.: New Records of Plant Community and 92/43/EEC Habitat in Italy

In this paper, integrating field surveys and literature data, an analysis of Ophioglossum lusitanicum plant communities and related 92/43/EEC habitats are reported for Italy. Two new syntaxa, Euphorbio exiguae-Ophioglossetum lusitanici ass. nova hoc loco and trifolietosum scabri subass. nova hoc loco of the Rumici bucephalophori-Ophioglossetum lusitanici were described in the Apulia and Campania regions.Both types of vegetation identified in Apulia, Campania, and Sicily regions represent two different aspects of the same priority habitat: “pseudo-steppe with grasses and annuals of the Thero-Brachypodietea” (habitat code 6220*). A phytosociological and ecological dataset of the literature and new field surveys highlighting the soil type as parameters affecting the vegetation cover of this small fernlike plant, with the Trachynion distachyae Rivas–Martínez, 1978 alliance on calcareous soils and Helianthemion guttati Br.-Bl. in Br.-Bl. et al., 1940 alliance on volcanic soils. Many species of other types of annual meadows have been identified within Ophioglossum communities due to the very small patches of land, where they have been found, and ecological conditions that facilitate this phenomenon of the transgression of other therophytes species