New Investigations in the Environment, History and Archaeology of the Iraqi Hilly Flanks: Shahrizor Survey Project 2009-2011

Recent palaeoenvironmental, historical, and archaeological investigations, primarily consisting of site reconnaissance, in the Shahrizor region within the province of Sulaymaniyah in Iraqi Kurdistan are bringing to light new information on the region’s social and socio-ecological development. This paper summarises two seasons of work by researchers from German, British, Dutch, and Iraqi-Kurdish institutions working in the survey region. Palaeoenvironmental data have determined that during the Pleistocene many terraces developed which came to be occupied by a number of the larger tell sites in the Holocene. In the sedimentary record, climatic and anthropogenic patterns are noticeable, and alluviation has affected the recovery of archaeological remains through site burial in places. Historical data show the Shahrizor shifting between periods of independence, either occupied by one regional state or several smaller entities, and periods that saw the plain’s incorporation within large empires, often in a border position. New archaeological investigations have provided insight into the importance of the region as a transit centre between Western Iran and northern and southern Mesopotamia, with clear material culture links recovered. Variations between periods’ settlement patterns and occupations are also beginning to emerge

Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper

<p>Abstract</p> <p>Background</p> <p>Bacterial spot of tomato and pepper is caused by four <it>Xanthomonas </it>species and is a major plant disease in warm humid climates. The four species are distinct from each other based on physiological and molecular characteristics. The genome sequence of strain 85-10, a member of one of the species, <it>Xanthomonas euvesicatoria </it>(<it>Xcv</it>) has been previously reported. To determine the relationship of the four species at the genome level and to investigate the molecular basis of their virulence and differing host ranges, draft genomic sequences of members of the other three species were determined and compared to strain 85-10.</p> <p>Results</p> <p>We sequenced the genomes of <it>X. vesicatoria </it>(<it>Xv</it>) strain 1111 (ATCC 35937), <it>X. perforans </it>(<it>Xp</it>) strain 91-118 and <it>X. gardneri </it>(<it>Xg</it>) strain 101 (ATCC 19865). The genomes were compared with each other and with the previously sequenced <it>Xcv </it>strain 85-10. In addition, the molecular features were predicted that may be required for pathogenicity including the type III secretion apparatus, type III effectors, other secretion systems, quorum sensing systems, adhesins, extracellular polysaccharide, and lipopolysaccharide determinants. Several novel type III effectors from <it>Xg </it>strain 101 and <it>Xv </it>strain 1111 genomes were computationally identified and their translocation was validated using a reporter gene assay. A homolog to Ax21, the elicitor of XA21-mediated resistance in rice, and a functional Ax21 sulfation system were identified in <it>Xcv</it>. Genes encoding proteins with functions mediated by type II and type IV secretion systems have also been compared, including enzymes involved in cell wall deconstruction, as contributors to pathogenicity.</p> <p>Conclusions</p> <p>Comparative genomic analyses revealed considerable diversity among bacterial spot pathogens, providing new insights into differences and similarities that may explain the diverse nature of these strains. Genes specific to pepper pathogens, such as the O-antigen of the lipopolysaccharide cluster, and genes unique to individual strains, such as novel type III effectors and bacteriocin genes, have been identified providing new clues for our understanding of pathogen virulence, aggressiveness, and host preference. These analyses will aid in efforts towards breeding for broad and durable resistance in economically important tomato and pepper cultivars.</p

Mapping and Identification of the RXopJ4 Resistance Gene and the Search for New Sources of Durable Resistance to Bacterial Spot Disease of Tomato

Bacterial spot of tomato (Solanum lycopersicum) is a devastating disease that severely limits yields in important tomato-­growing regions, including the southeastern United States, where the predominant bacterial spot pathogen species is Xanthomonas perforans. Attempts to control the disease with antibiotics and copper-­based pesticides have led to the selection of bacterial strains that are resistant to these treatments. Therefore, we turn to genetic sources of resistance as a sustainable path to reduce crop losses to bacterial spot disease.This work describes the fine mapping and identification of the RXopJ4 disease resistance locus from the wild tomato relative Solanum pennellii LA716. RXopJ4 resistance depends on recognition of the X. perforans type III effector protein XopJ4. We developed a collection of fourteen molecular markers to map on a segregating F2 population from a cross between the susceptible parent S. lycopersicum FL8000 and the resistant parent RXopJ4 8000 OC7. In the F2 population, a 190-­kb segment on chromosome 6 cosegregated with resistance. This fine mapping enabled the identification of three RXopJ4 candidate genes, all of which encode putative intracellular serine-­threonine protein kinases. Transient coexpression of the XopJ4 effector with each kinase revealed a promising RXopJ4 candidate gene that triggered a hypersensitive response (HR) in Nicotiana benthamiana. Mutations in both XopJ4 and RXopJ4 identified conserved residues required for recognition and the induction of a hypersensitive cell death phenotype. Homozygous transgenic tomato plants containing the RXopJ4 candidate gene have been constructed and will soon be evaluated for disease resistance.In addition, we undertook a genomic survey of fourteen X. perforans field isolates from all five fresh market tomato production zones of Florida, revealing a preliminary set of core type III effectors common to all isolates. We used this set of core effectors to inform a search for new sources of resistance to bacterial spot disease and identified accessions of Nicotiana and Solanum americanum that recognize core X. perforans effectors. Finally, we performed disease assays on 224 wild tomato accessions and found nine potential sources of bacterial spot disease resistance

Peer support for family carers of people with dementia, alone or in combination with group reminiscence in a factorial design: study protocol for a randomised controlled trial

Background Peer support interventions can improve carer wellbeing and interventions that engage both the carer and person with dementia can have significant mutual benefits. Existing research has been criticised for inadequate rigour of design or reporting. This paper describes the protocol for a complex trial that evaluates one-to-one peer support and a group reminiscence programme, both separately and together, in a factorial design. Design A 2 × 2 factorial multi-site randomised controlled trial of individual peer support and group reminiscence interventions for family carers and people with dementia in community settings in England, addressing both effectiveness and cost-effectiveness. Discussion The methods described in this protocol have implications for research into psychosocial interventions, particularly complex interventions seeking to test both individual and group approaches

Fine genetic mapping of RXopJ4, a bacterial spot disease resistance locus from Solanum pennellii LA716

The RXopJ4 resistance locus from the wild accession Solanum pennellii (Sp) LA716 confers resistance to bacterial spot disease of tomato (S. lycopersicum, Sl) caused by Xanthomonas perforans (Xp). RXopJ4 resistance depends on recognition of the pathogen type III effector protein XopJ4. We used a collection of Sp introgression lines (ILs) to narrow the RXopJ4 locus to a 4.2-Mb segment on the long arm of chromosome 6, encompassed by the ILs 6-2 and 6-2-2. We then adapted or developed a collection of 14 molecular markers to map on a segregating F(2) population from a cross between the susceptible parent Sl FL8000 and the resistant parent RXopJ4 8000 OC(7). In the F(2) population, a 190-kb segment between the markers J350 and J352 cosegregated with resistance. This fine mapping will enable both the identification of candidate genes and the detection of resistant plants using cosegregating markers. The RXopJ4 resistance gene(s), in combination with other recently characterized genes and a quantitative trait locus (QTL) for bacterial spot disease resistance, will likely be an effective tool for the development of durable resistance in cultivated tomato

The New Age of Security: Implications for refugees and internally displaced persons in the Horn of Africa

Cawo M. Abdi looks at the Somali crisis as the most recent conflict being fought in the name of ‘war against terrorism’. She argues that it entails major implications for migration in general and for migrant women in particular. Her central concerns are to track the new realities in the various forms of migration across the world and to better understand at a policy level how migration and development nexus encourages south–north, south–south social and economic relations with more open and accountable development paths. She tracks how the migration and development nexus challenges concepts of citizenship, trans-national borders, diversity, social protection and security. Development (2007) 50, 75–81. doi:10.1057/palgrave.development.1100434