38 research outputs found
Recommended from our members
Mechanisms of organelle biogenesis govern stochastic fluctuations in organelle abundance
Fluctuations in organelle abundance can profoundly limit the precision of cell biological processes from secretion to metabolism. We modeled the dynamics of organelle biogenesis and predicted that organelle abundance fluctuations depend strongly on the specific mechanisms that increase or decrease the number of a given organelle. Our model exactly predicts the size of experimentally measured Golgi apparatus and vacuole abundance fluctuations, suggesting that cells tolerate the maximum level of variability generated by the Golgi and vacuole biogenesis pathways. We observe large increases in peroxisome abundance fluctuations when cells are transferred from glucose-rich to fatty acid-rich environments. These increased fluctuations are significantly diminished in mutants lacking peroxisome fission factors, leading us to infer that peroxisome biogenesis switches from de novo synthesis to primarily fission. Our work provides a general framework for exploring stochastic organelle biogenesis and using fluctuations to quantitatively unravel the biophysical pathways that control the abundance of subcellular structures. DOI: http://dx.doi.org/10.7554/eLife.02678.00
The dynamics of enzymatic switch cascades
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Physics; and, (S.B.)--Massachusetts Institute of Technology, Dept. of Mathematics, 2004.Includes bibliographical references (leaf 67).We examine the dynamics of the mitogen-activated protein kinase (MAPK) multi-step enzymatic switching cascade, a highly conserved architecture utilised in cellular signal transduction. In treating the equations of motion, we replace the usual deterministic differential equation formalism with stochastic equations to accurately model the 'effective collisions' picture of the biochemical reactions that constitute the network. Furthermore we measure the fidelity of the signaling process through the mutual information content between the output of a given switch and the original environmental input to the system. We find that the enzymatic switches act as low-pass filters, with each switch in the cascade able to average over high frequency stochastic fluctuations in the network and throughput cleaner signals to downstream switches. We find optimal regions of mutual information transfer with respect to reaction velocity and species number parameters, and observe the dynamical memory-gain and memory-loss as well as decay in mutual information in quadruple-linked switch systems.by Shankar Mukherji.S.B
Recommended from our members
Robust Circadian Oscillations in Growing Cyanobacteria Require Transcriptional Feedback
The remarkably stable circadian oscillations of single cyanobacteria enable a population of growing cells to maintain synchrony for weeks. The cyanobacterial pacemaker is a posttranslational regulation (PTR) circuit that generates circadian oscillations in the phosphorylation state of the clock protein KaiC. Layered on top of the PTR is transcriptional-translational feedback regulation (TTR), common to all circadian systems, consisting of a negative feedback loop in which KaiC regulates its own production. We found that the PTR circuit is sufficient to generate oscillations in growing cyanobacteria. However, in the absence of TTR, individual oscillators were less stable and synchrony was not maintained in a population of cells. Experimentally constrained mathematical modeling reproduced sustained oscillations in the PTR circuit alone and demonstrated the importance of TTR for oscillator synchrony.Chemistry and Chemical BiologyMolecular and Cellular BiologyPhysic
Optically-Trapped Nanodiamond-Relaxometry Detection of Nanomolar Paramagnetic Spins in Aqueous Environments
Probing electrical and magnetic properties in aqueous environments remains a
frontier challenge in nanoscale sensing. Our inability to do so with
quantitative accuracy imposes severe limitations, for example, on our
understanding of the ionic environments in a diverse array of systems, ranging
from novel materials to the living cell. The Nitrogen-Vacancy (NV) center in
fluorescent nanodiamonds (FNDs) has emerged as a good candidate to sense
temperature, pH, and the concentration of paramagnetic species at the
nanoscale, but comes with several hurdles such as particle-to-particle
variation which render calibrated measurements difficult, and the challenge to
tightly confine and precisely position sensors in aqueous environment. To
address this, we demonstrate relaxometry with NV centers within
optically-trapped FNDs. In a proof of principle experiment, we show that
optically-trapped FNDs enable highly reproducible nanomolar sensitivity to the
paramagnetic ion, (\mathrm{Gd}^{3+}). We capture the three distinct phases of
our experimental data by devising a model analogous to nanoscale Langmuir
adsorption combined with spin coherence dynamics. Our work provides a basis for
routes to sense free paramagnetic ions and molecules in biologically relevant
conditions.Comment: 6+7 pages, 3+8 figure
MicroRNAs can generate thresholds in target gene expression
MicroRNAs (miRNAs) are short, highly conserved noncoding RNA molecules that repress gene expression in a sequence-dependent manner. We performed single-cell measurements using quantitative fluorescence microscopy and flow cytometry to monitor a target gene's protein expression in the presence and absence of regulation by miRNA. We find that although the average level of repression is modest, in agreement with previous population-based measurements, the repression among individual cells varies dramatically. In particular, we show that regulation by miRNAs establishes a threshold level of target mRNA below which protein production is highly repressed. Near this threshold, protein expression responds sensitively to target mRNA input, consistent with a mathematical model of molecular titration. These results show that miRNAs can act both as a switch and as a fine-tuner of gene expression.National Institutes of Health (U.S.). Director's Pioneer Award (1DP1OD003936)National Cancer Institute (U.S.). Physical Sciences-Oncology Center (U54CA143874)United States. Public Health Service (Grant R01-CA133404)United States. Public Health Service (Grant R01-GM34277)National Cancer Institute (U.S.) (PO1-CA42063)National Cancer Institute (U.S.) Cancer Center Support (Grant P30-CA14051)Howard Hughes Medical Institute. Predoctoral FellowshipCleo and Paul Schimmel Foundation. FellowshipNatural Sciences and Engineering Research Council of Canada PGS Scholarshi
Synthetic biology: Understanding biological design from synthetic circuits
An important aim of synthetic biology is to uncover the design principles of natural biological systems through the rational design of gene and protein circuits. Here, we highlight how the process of engineering biological systems — from synthetic promoters to the control of cell–cell interactions — has contributed to our understanding of how endogenous systems are put together and function. Synthetic biological devices allow us to grasp intuitively the ranges of behaviour generated by simple biological circuits, such as linear cascades and interlocking feedback loops, as well as to exert control over natural processes, such as gene expression and population dynamics