307 research outputs found
From everyday conversation to political action: Talking austerity in online âthird spacesâ
- Author
- Publication venue
- 'SAGE Publications'
- Publication date
- 01/01/2015
- Field of study
Taking forward a new agenda for online political deliberation â the study of everyday political talk in non-political, online âthird spacesâ â this article examines the dynamics of political talk across three general interest UK-based online forums. The quantitative analysis found that discussions about austerity were just as likely to emerge from non-political discussions as they were ones that began as âpoliticalâ, demonstrating the links people made between everyday experiences and public policy. Our qualitative analysis represents the first real attempt to analyse political actions within third spaces, with some striking outcomes. Over half of all political discussions led to at least one political action (with significant variation between forums). The findings demonstrate that while such third spaces remain concerned with the preoccupations of everyday life, they can and do perform a role of mobilizing agent towards political participation
Reliability analysis of the Ahringer Caenorhabditis elegans RNAi feeding library: a guide for genome-wide screens
- Author
- Publication venue
- BioMed Central
- Publication date
- 01/01/2011
- Field of study
<p>Abstract</p> <p>Background</p> <p>The Ahringer <it>C. elegans </it>RNAi feeding library prepared by cloning genomic DNA fragments has been widely used in genome-wide analysis of gene function. However, the library has not been thoroughly validated by direct sequencing, and there are potential errors, including: 1) mis-annotation (the clone with the retired gene name should be remapped to the actual target gene); 2) nonspecific PCR amplification; 3) cross-RNAi; 4) mis-operation such as sample loading error, <it>etc</it>.</p> <p>Results</p> <p>Here we performed a reliability analysis on the Ahringer <it>C. elegans </it>RNAi feeding library, which contains 16,256 bacterial strains, using a bioinformatics approach. Results demonstrated that most (98.3%) of the bacterial strains in the library are reliable. However, we also found that 2,851 (17.54%) bacterial strains need to be re-annotated even they are reliable. Most of these bacterial strains are the clones having the retired gene names. Besides, 28 strains are grouped into unreliable category and 226 strains are marginal because of probably expressing unrelated double-stranded RNAs (dsRNAs). The accuracy of the prediction was further confirmed by direct sequencing analysis of 496 bacterial strains. Finally, a freely accessible database named CelRNAi (<url>http://biocompute.bmi.ac.cn/CelRNAi/</url>) was developed as a valuable complement resource for the feeding RNAi library by providing the predicted information on all bacterial strains. Moreover, submission of the direct sequencing result or any other annotations for the bacterial strains to the database are allowed and will be integrated into the CelRNAi database to improve the accuracy of the library. In addition, we provide five candidate primer sets for each of the unreliable and marginal bacterial strains for users to construct an alternative vector for their own RNAi studies.</p> <p>Conclusions</p> <p>Because of the potential unreliability of the Ahringer <it>C. elegans </it>RNAi feeding library, we strongly suggest the user examine the reliability information of the bacterial strains in the CelRNAi database before performing RNAi experiments, as well as the post-RNAi experiment analysis.</p
Long-term follow-up of beryllium sensitized workers from a single employer
- Author
- American Thoracic Society
- American Thoracic Society
- Anne M Curtis
- AW Stange
- C Saltini
- CR Schuler
- David C Deubner
- DC Deubner
- EP Donovan
- JM Aronchick
- K Kreiss
- K Kreiss
- K Kreiss
- K Kreiss
- K Rosenman
- LA Maier
- LA Maier
- LS Newman
- LS Newman
- LS Newman
- Mark R Cullen
- MD Rossman
- Mona Duggal
- MR Cullen
- MR Cullen
- MR Cullen
- PK Henneberger
- PK Henneberger
- RJ Knudson
- RO Crapo
- TN Markham
- Publication venue
- BioMed Central
- Publication date
- 01/01/2010
- Field of study
<p>Abstract</p> <p>Background</p> <p>Up to 12% of beryllium-exposed American workers would test positive on beryllium lymphocyte proliferation test (BeLPT) screening, but the implications of sensitization remain uncertain.</p> <p>Methods</p> <p>Seventy two current and former employees of a beryllium manufacturer, including 22 with pathologic changes of chronic beryllium disease (CBD), and 50 without, with a confirmed positive test were followed-up for 7.4 +/-3.1 years.</p> <p>Results</p> <p>Beyond predicted effects of aging, flow rates and lung volumes changed little from baseline, while D<sub>L</sub>CO dropped 17.4% of predicted on average. Despite this group decline, only 8 subjects (11.1%) demonstrated physiologic or radiologic abnormalities typical of CBD. Other than baseline status, no clinical or laboratory feature distinguished those who clinically manifested CBD at follow-up from those who did not.</p> <p>Conclusions</p> <p>The clinical outlook remains favorable for beryllium-sensitized individuals over the first 5-12 years. However, declines in D<sub>L</sub>CO may presage further and more serious clinical manifestations in the future. These conclusions are tempered by the possibility of selection bias and other study limitations.</p
Deregulation of apoptosis mediators' p53 and bcl2 in lung tissue of COPD patients
- Author
- A Agusti
- AM Vignola
- C Jiménez-Ruiz
- CR Jenkins
- CV Weaver
- CV Weaver
- D Makris
- DA Martin
- DL Bratton
- DP Tashkin
- E Tzortzaki
- EM Vlachaki
- IK Demedts
- JW Park
- K Ikeda
- K Imai
- KF Rabe
- M Plataki
- M Schuler
- MA Pahlavani
- N Yokohori
- NE Alexis
- PM de Souza
- PM Henson
- RM Tuder
- RP Schleimer
- RS Mascaretti
- S Haupt
- S Hodge
- S Hodge
- S Hodge
- S Hodge
- S Hodge
- S Hodge
- SJ Hodge
- Y Kasahara
- Publication venue
- BioMed Central
- Publication date
- 01/04/2010
- Field of study
Abnormal apoptotic events in chronic obstructive pulmonary disease (COPD) subvert cellular homeostasis and may play a primary role in its pathogenesis. However, studies in human subjects are limited
Jet energy measurement with the ATLAS detector in proton-proton collisions at root s=7 TeV
- Author
- Aad G
- Abbott B
- Abdallah J
- Abdelalim AA
- Abdesselam A
- Abdinov O
- Abi B
- Abolins M
- Abramowicz H
- Abreu H
- Acerbi E
- Acharya BS
- Adams DL
- Addy TN
- Adelman J
- Aderholz M
- Adomeit S
- Adragna P
- Adye T
- Aefsky S
- Aguilar-Saavedra JA
- Aharrouche M
- Ahlen SP
- Ahles F
- Ahmad A
- Ahsan M
- Aielli G
- Akdogana T
- Akesson TPA
- Akimoto G
- Akimov AV
- Akiyama A
- Aktas A
- Alam MA
- Alam MS
- Albert J
- Albrand S
- Aleksa M
- Aleksandrov IN
- Alessandria F
- Alexa C
- Alexander G
- Alexandre G
- Alexopoulos T
- Alhroob M
- Aliev M
- Alimonti G
- Alison J
- Aliyev M
- Allport PP
- Allwood-Spiers SE
- Almenar CC
- Almond J
- Aloisio A
- Alon R
- Alonso A
- Alviggi MG
- Amako K
- Amaral P
- Amelung C
- Ammosov VV
- Amorim A
- Amoros G
- Amram N
- Anastopoulos C
- Ancu LS
- Andari N
- Andeen T
- Anders CF
- Anders G
- Anderson KJ
- Andreazza A
- Andrei V
- Andrieux M-L
- Anduaga XS
- Angerami A
- Anghinolfi F
- Anh TV
- Anjos N
- Annovi A
- Antonaki A
- Antonelli M
- Antonov A
- Antos J
- Anulli F
- Aoun S
- Apolle R
- Arabidze G
- Aracena I
- Arai Y
- Aranda CP
- Arce ATH
- Archambault JP
- Arfaoui S
- Arguin J-F
- Arik E
- Arik M
- Armbruster AJ
- Arnaez O
- Arnault C
- Artamonov A
- Artoni G
- Arutinov D
- Asai S
- Asfandiyarov R
- Ask S
- Asman B
- Asner D
- Asquith L
- Assamagan K
- Astbury A
- Astvatsatourov A
- Atoian G
- Aubert B
- Auge E
- Augsten K
- Aurousseau M
- Austin N
- Avolio G
- Avramidou R
- Axen D
- Ay C
- Azuelos G
- Azuma Y
- Baak MA
- Baccaglioni G
- Bacci C
- Bach AM
- Bachacou H
- Bachas K
- Bachy G
- Backes M
- Backhaus M
- Badescu E
- Bagnaia P
- Bahinipati S
- Bai Y
- Bailey DC
- Bain T
- Baines JT
- Baker MD
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- Bangert A
- Bansal V
- Bansil HS
- Barajas CAC
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- Barashkou A
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- Bardin DY
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- Belenguer MJ
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- Zhemchugov A
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- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/03/2013
- Field of study
The jet energy scale and its systematic uncertainty are determined for jets measured with the ATLAS detector at the LHC in proton-proton collision data at a centre-of-mass energy of âs = 7TeV corresponding to an integrated luminosity of 38 pb-1. Jets are reconstructed with the anti-kt algorithm with distance parameters R=0. 4 or R=0. 6. Jet energy and angle corrections are determined from Monte Carlo simulations to calibrate jets with transverse momenta pTâ„20 GeV and pseudorapidities {pipe}η{pipe}<4. 5. The jet energy systematic uncertainty is estimated using the single isolated hadron response measured in situ and in test-beams, exploiting the transverse momentum balance between central and forward jets in events with dijet topologies and studying systematic variations in Monte Carlo simulations. The jet energy uncertainty is less than 2. 5 % in the central calorimeter region ({pipe}η{pipe}<0. 8) for jets with 60â€pT<800 GeV, and is maximally 14 % for pT<30 GeV in the most forward region 3. 2â€{pipe}η{pipe}<4. 5. The jet energy is validated for jet transverse momenta up to 1 TeV to the level of a few percent using several in situ techniques by comparing a well-known reference such as the recoiling photon pT, the sum of the transverse momenta of tracks associated to the jet, or a system of low-pT jets recoiling against a high-pT jet. More sophisticated jet calibration schemes are presented based on calorimeter cell energy density weighting or hadronic properties of jets, aiming for an improved jet energy resolution and a reduced flavour dependence of the jet response. The systematic uncertainty of the jet energy determined from a combination of in situ techniques is consistent with the one derived from single hadron response measurements over a wide kinematic range. The nominal corrections and uncertainties are derived for isolated jets in an inclusive sample of high-pT jets. Special cases such as event topologies with close-by jets, or selections of samples with an enhanced content of jets originating from light quarks, heavy quarks or gluons are also discussed and the corresponding uncertainties are determined. © 2013 CERN for the benefit of the ATLAS collaboration
Measurement of the inclusive and dijet cross-sections of b-jets in pp collisions at sqrt(s) = 7 TeV with the ATLAS detector
- Author
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- Abbott B
- Abdallah J
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- Abdesselam A
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- Zwalinski L
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 31/12/2010
- Field of study
The inclusive and dijet production cross-sections have been measured for jets
containing b-hadrons (b-jets) in proton-proton collisions at a centre-of-mass
energy of sqrt(s) = 7 TeV, using the ATLAS detector at the LHC. The
measurements use data corresponding to an integrated luminosity of 34 pb^-1.
The b-jets are identified using either a lifetime-based method, where secondary
decay vertices of b-hadrons in jets are reconstructed using information from
the tracking detectors, or a muon-based method where the presence of a muon is
used to identify semileptonic decays of b-hadrons inside jets. The inclusive
b-jet cross-section is measured as a function of transverse momentum in the
range 20 < pT < 400 GeV and rapidity in the range |y| < 2.1. The bbbar-dijet
cross-section is measured as a function of the dijet invariant mass in the
range 110 < m_jj < 760 GeV, the azimuthal angle difference between the two jets
and the angular variable chi in two dijet mass regions. The results are
compared with next-to-leading-order QCD predictions. Good agreement is observed
between the measured cross-sections and the predictions obtained using POWHEG +
Pythia. MC@NLO + Herwig shows good agreement with the measured bbbar-dijet
cross-section. However, it does not reproduce the measured inclusive
cross-section well, particularly for central b-jets with large transverse
momenta.Comment: 10 pages plus author list (21 pages total), 8 figures, 1 table, final
version published in European Physical Journal
Expected Performance of the ATLAS Experiment - Detector, Trigger and Physics
- Author
- \uc5kesson T P A
- \uc5sman B
- Aad G
- Abat E
- Abbott B
- Abdallah J
- Abdelalim A A
- Abdesselam A
- Abdinov O
- Abi B
- Abolins M
- Abramowicz H
- Acharya B S
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- Adragna P
- Adye T
- Aguilar-Saavedra J A
- Aharrouche M
- Ahlen S P
- Ahles F
- Ahmad A
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- Aielli G
- Akdogan T
- Akimoto G
- Alam M A
- Alam S M
- Albert J
- Albrand S
- Aleksa M
- Aleksandrov I N
- Alessandria F
- Alexa C
- Alexander G
- Alexandre G
- Alexopoulos T
- Alhroob M
- Alimonti G
- Alison J
- Aliyev M
- Allport P P
- Allwood-Spiers S E
- Aloisio A
- Alon R
- Alonso A
- Alonso J
- Alviggi M G
- Amako K
- Amaral P
- Amelung C
- Ammosov V V
- Amor\uf3s G
- Amorim A
- Amram N
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- Anderson K J
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- Zenonos Z
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- Zitoun R
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- Zmouchko V V
- Zobernig G
- Zoccoli A
- zur Nedden M
- Zychacek V
- Publication venue
- Publication date
- 01/01/2008
- Field of study
A detailed study is presented of the expected performance of the ATLAS
detector. The reconstruction of tracks, leptons, photons, missing energy and
jets is investigated, together with the performance of b-tagging and the
trigger. The physics potential for a variety of interesting physics processes,
within the Standard Model and beyond, is examined. The study comprises a series
of notes based on simulations of the detector and physics processes, with
particular emphasis given to the data expected from the first years of
operation of the LHC at CERN
Nonsense Mediated Decay Resistant Mutations Are a Source of Expressed Mutant Proteins in Colon Cancer Cell Lines with Microsatellite Instability
- Author
- A Duval
- A Leibovitz
- A Weise
- Antony W. Burgess
- B Drewinko
- Ben C. B. Ko
- BH Tom
- C Engel
- CR Boland
- CR Boland
- D Middleton
- D Tougeron
- David S. Williams
- E Gasteiger
- E Ripberger
- Edouard C. Nice
- EM Stoffel
- EY Chang
- F Pastor
- FM Speetjens
- Franscesa Walker
- G Des Guetz
- HH Zhang
- HT Lynch
- Hui Hua Zhang
- I Saeterdal
- I Saeterdal
- J El-Bchiri
- J Wen
- KS Cresswell
- KT You
- L Berglund
- L Shen
- M Linnebacher
- M Linnebacher
- M Reuschenbach
- MA Jenkins
- Matthew J. Bird
- MG Brattain
- MM Schuler
- RH Whitehead
- RH Whitehead
- RH Whitehead
- RN Jorissen
- Robert N. Jorissen
- S Ogino
- S Popat
- S von Kleist
- SA Bader
- SC Kirkland
- SM Andrew
- SM Phillips
- SM Woerner
- SM Woerner
- SM Woerner
- T Ishikawa
- T Watanabe
- TJ Hubbard
- Y Ionov
- Y Ionov
- Y Niv
- Y Schwitalle
- Y Schwitalle
- Y Sykulev
- Yen Lin Yu
- YF Chang
- Publication venue
- Public Library of Science
- Publication date
- 31/12/2010
- Field of study
BACKGROUND: Frameshift mutations in microsatellite instability high (MSI-High) colorectal cancers are a potential source of targetable neo-antigens. Many nonsense transcripts are subject to rapid degradation due to nonsense-mediated decay (NMD), but nonsense transcripts with a cMS in the last exon or near the last exon-exon junction have intrinsic resistance to nonsense-mediated decay (NMD). NMD-resistant transcripts are therefore a likely source of expressed mutant proteins in MSI-High tumours. METHODS: Using antibodies to the conserved N-termini of predicted mutant proteins, we analysed MSI-High colorectal cancer cell lines for examples of naturally expressed mutant proteins arising from frameshift mutations in coding microsatellites (cMS) by immunoprecipitation and Western Blot experiments. Detected mutant protein bands from NMD-resistant transcripts were further validated by gene-specific short-interfering RNA (siRNA) knockdown. A genome-wide search was performed to identify cMS-containing genes likely to generate NMD-resistant transcripts that could encode for antigenic expressed mutant proteins in MSI-High colon cancers. These genes were screened for cMS mutations in the MSI-High colon cancer cell lines. RESULTS: Mutant protein bands of expected molecular weight were detected in mutated MSI-High cell lines for NMD-resistant transcripts (CREBBP, EP300, TTK), but not NMD-sensitive transcripts (BAX, CASP5, MSH3). Expression of the mutant CREBBP and EP300 proteins was confirmed by siRNA knockdown. Five cMS-bearing genes identified from the genome-wide search and without existing mutation data (SFRS12IP1, MED8, ASXL1, FBXL3 and RGS12) were found to be mutated in at least 5 of 11 (45%) of the MSI-High cell lines tested. CONCLUSION: NMD-resistant transcripts can give rise to expressed mutant proteins in MSI-High colon cancer cells. If commonly expressed in primary MSI-High colon cancers, MSI-derived mutant proteins could be useful as cancer specific immunological targets in a vaccine targeting MSI-High colonic tumours
Effectiveness of a physical activity program on cardiovascular disease risk in adult primary health-care users: the âPas-a-Pasâ community intervention trial
- Author
- A Kouvonen
- AB Segerström
- AH Tiessen
- Angels Vinuesa
- BA Lawton
- C Besler
- CJ Salinas
- CP Wen
- CR Elley
- D Stensvold
- Dolors Aguas
- E Andersen
- EMF Sluijs Van
- Felipe Villalobos
- G Schuler
- GuĂa Europea de PrevenciĂłn Cardiovascular en la PrĂĄctica ClĂnica
- H Liira
- HW Kohl
- IT RodrĂguez
- J Halbert
- J Kim
- J Marrugat
- J Myers
- J Truthmann
- JM Rippe
- Josep Basora
- LC Dalleck
- M Hamer
- M Otero
- Mercé Timón
- MF Piepoli
- MH Murphy
- OK Duru
- R Ramos
- Roser Pedret
- S Mora
- SA Claas
- SC Dumith
- SM Hegde
- Teresa Basora
- V Arija
- Victoria Arija
- WL Haskell
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
From Words to Images Through Legal Visualization
- Author
- Publication venue
- Publication date
- 01/11/2018
- Field of study
One of the common characteristics of legal documents is the absolute preponderance of text and their specific domain language, whose complexity can result in impenetrability for those that have no legal expertise. In some experiments, visual communication has been introduced in legal documents to make their meaning clearer and more intelligible, whilst visualizations have also been automatically generated from semantically-enriched legal data. As part of an ongoing research that aims to create user-friendly privacy terms by integrating graphical elements and Semantic Web technologies, the process of creation and interpretation of visual legal concepts will be discussed. The analysis of current approaches to this subject represents the point of departure to propose an empirical methodology that is inspired by interaction and human-centered design practices
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