Web-based Aptitude Tests at Universities in German-speaking Countries

Universities can select their students more and more independently. In order to support the selection process, web-based aptitude tests are a possibility to balance benefits und efforts of this task. Within this paper, we will point out how current web-based aptitude tests are designed, what competences are covered, and which methods for development are used. For this purpose, we developed a classification how web-based aptitude tests are implemented. Furthermore, competences as the basis of web-based aptitude tests are appraised. Four competence categories (professional, methodological, personal, and social competences) are selected as the most appropriate pattern. Thereafter, we analyse methods for developing competence specifications. Finally, we state lessons learned for the development of web-based aptitude tests at universities. These results are an important preparatory work and a basis for a systematically development of a web-based aptitude test for the University of Erlangen-Nuremberg

Evaluation of User Acceptance for Web-based Aptitude Tests

Web-based aptitude tests, which are a special category of aptitude tests, can be used for rather standardized test methods and for a large amount of users. The characteristics of web-based aptitude tests can have an impact on the test results and the user acceptance. The aim of our research is to develop a method for the evaluation of the user acceptance for web-based aptitude tests. Therefore, we used the DART-approach with the dimension (Perceived) Usefulness, (Perceived) Ease of Use, (Perceived) Network Effects and (Perceived) Costs as the theoretical basis, identified important acceptance indicators, developed a questionnaire and conducted a survey. Afterwards, we proved the reliability and conducted a factor analysis. The results point out that some of the defined acceptance indicators should be revised. Additionally, the factor analysis shows that a combination of two dimensions (Perceived) Usefulness and (Perceived) Network Effects is useful especially with regard to web-based aptitude tests. Finally, we conducted a univariate analysis to evaluate the user acceptance of a web-based aptitude test. The visualised result on the basis of a DART-chart clearly shows that the interviewees evaluated the indicators very differently. There are fields, where the aptitude test fulfils the expectations, and fields, which can be improved

Testing intermediate-age stellar evolution models with VLT photometry of LMC clusters. II. Analysis with the Yale models

We present an analysis of CMDs of three intermediate-age LMC clusters, namely NGC 2173, SL 556 and NGC 2155. The main goal of our project is to investigate the amount of convective core overshoot necessary to reproduce the CMDs of relatively metal-poor, intermediate age stellar populations. We conclude that a moderate amount of overshoot and some fraction of binary stars are essential for reproducing the observed shapes around the turnoff in the CMD's of all three clusters: unresolved binary stars fill in the expected core contraction gap, and make a unique sequence near the gap, which cannot be reproduced by single stars alone, even with a larger amount of overshoot. From our overall analysis such as, shape of isochrones, star counts, color distribution, and synthetic CMD comparisons, we conclude that overshoot ~ 20% of the local pressure scale height best reproduces the CMD properties of all three clusters. The best age estimates are 1.5, 2.1 and 2.9 Gyr for NGC 2173, SL 556 and NGC 2155, respectively.Comment: 19 pages, 13 figures, accepted for the publication in A

Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells

Background: Although p75 neurotrophin receptor (p75NTR) is the first neurotrophin receptor isolated, its diverse physiological functions and signaling have remained elusive for many years. Loss-of-function phenotypic analyses for p75NTR were mainly focused at the genetic level; however these approaches were impacted by off-target effect, insufficient stability, unspecific stress response or alternative active splicing products. In this study, p75NTR surface expression was suppressed for the first time at the protein level by endoplasmic reticulum (ER) retained intrabodies. Results: Three monoclonal recombinant antibody fragments (scFv) with affinities in the low nanomolar range to murine p75NTR were isolated by antibody phage display. To suppress p75NTR cell surface expression, the encoding genes of these scFvs extended by the ER retention peptide KDEL were transiently transfected into the neuron-like rat pheochromocytoma cell line PC12 and the mouse neuroblastoma x mouse spinal cord hybrid cell line NSC19. The ER retained intrabody construct, SH325-G7-KDEL, mediated a downregulation of p75NTR cell surface expression as shown by flow cytometry. This effect was maintained over a period of at least eight days without activating an unfolded protein response (UPR). Moreover, the ER retention of p75NTR resulted in downregulation of mRNA levels of the anti-apoptotic protein Bcl-xL as well as in strong inhibition of NGF-induced neurite outgrowth in PC12 cells. Conclusion: The ER retained intrabody SH325-G7-KDEL not only induces phenotypic knockdown of this p75NTR but als

Germline variation at 8q24 and prostate cancer risk in men of European ancestry

Chromosome 8q24 is a susceptibility locus for multiple cancers, including prostate cancer. Here we combine genetic data across the 8q24 susceptibility region from 71,535 prostate cancer cases and 52,935 controls of European ancestry to define the overall contribution of germline variation at 8q24 to prostate cancer risk. We identify 12 independent risk signals for prostate cancer (p < 4.28 × 10−15), including three risk variants that have yet to be reported. From a polygenic risk score (PRS) model, derived to assess the cumulative effect of risk variants at 8q24, men in the top 1% of the PRS have a 4-fold (95%CI = 3.62–4.40) greater risk compared to the population average. These 12 variants account for ~25% of what can be currently explained of the familial risk of prostate cancer by known genetic risk factors. These findings highlight the overwhelming contribution of germline variation at 8q24 on prostate cancer risk which has implications for population risk stratification

Fine-mapping of prostate cancer susceptibility loci in a large meta-analysis identifies candidate causal variants

Prostate cancer is a polygenic disease with a large heritable component. A number of common, low-penetrance prostate cancer risk loci have been identified through GWAS. Here we apply the Bayesian multivariate variable selection algorithm JAM to fine-map 84 prostate cancer susceptibility loci, using summary data from a large European ancestry meta-analysis. We observe evidence for multiple independent signals at 12 regions and 99 risk signals overall. Only 15 original GWAS tag SNPs remain among the catalogue of candidate variants identified; the remainder are replaced by more likely candidates. Biological annotation of our credible set of variants indicates significant enrichment within promoter and enhancer elements, and transcription factor-binding sites, including AR, ERG and FOXA1. In 40 regions at least one variant is colocalised with an eQTL in prostate cancer tissue. The refined set of candidate variants substantially increase the proportion of familial relative risk explained by these known susceptibility regions, which highlights the importance of fine-mapping studies and has implications for clinical risk profiling. © 2018 The Author(s).Prostate cancer is a polygenic disease with a large heritable component. A number of common, low-penetrance prostate cancer risk loci have been identified through GWAS. Here we apply the Bayesian multivariate variable selection algorithm JAM to fine-map 84 prostate cancer susceptibility loci, using summary data from a large European ancestry meta-analysis. We observe evidence for multiple independent signals at 12 regions and 99 risk signals overall. Only 15 original GWAS tag SNPs remain among the catalogue of candidate variants identified; the remainder are replaced by more likely candidates. Biological annotation of our credible set of variants indicates significant enrichment within promoter and enhancer elements, and transcription factor-binding sites, including AR, ERG and FOXA1. In 40 regions at least one variant is colocalised with an eQTL in prostate cancer tissue. The refined set of candidate variants substantially increase the proportion of familial relative risk explained by these known susceptibility regions, which highlights the importance of fine-mapping studies and has implications for clinical risk profiling. © 2018 The Author(s).Peer reviewe

Antioxidant effects of selegiline in oxidative stress induced by iron neonatal treatment in rats

Increased levels of iron in specific brain regions have been reported in neurodegenerative disorders. It has been postulated that iron exerts its deleterious effects on the nervous system by inducing oxidative damage. In a previous study, we have shown that iron administered during a particular period of the neonatal life induces oxidative damage in brain regions in adult rats. The aim of the present study was to evaluate the possible protective effect of selegiline, a monoamino-oxidase B (MAO-B) inhibitor used in pharmacotherapy of Parkinson’s disease, against ironinduced oxidative stress in the brain. Results have shown that selegiline (1.0 and 10.0 mg/kg), when administered early in life was able to protect the substantia nigra as well as the hippocampus against iron-induced oxidative stress, without affecting striatum. When selegiline (10.0 mg/kg) was administered in the adult life to iron-treated rats, oxidative stress was reduced only in the substantia nigra

Citotoxicidade do haloperidol mediada por radicais livres

10

Loss of Y-Chromosome during Male Breast Carcinogenesis

Loss of Y-chromosome (LOY) is associated with increased cancer mortality in males. The prevalence of LOY in male breast cancer (BC) is unknown. The aim of this study is to assess the presence and prognostic effect of LOY during male BC progression. We included male BC patients diagnosed between 1989 and 2009 (n = 796). A tissue microarray (TMA) was constructed to perform immunohistochemistry and fluorescent in situ hybridization (FISH), using an X and Y probe. We also performed this FISH on a selected number of patients using whole tissue slides to study LOY during progression from ductal carcinoma in situ (DCIS) to invasive BC. In total, LOY was present in 12.7% (n = 92) of cases, whereby LOY was associated with ER and PR negative tumors (p = 0.017 and p = 0.01). LOY was not associated with the outcome. Using whole slides including invasive BC and adjacent DCIS (n = 22), we detected a concordant LOY status between both components in 17 patients. In conclusion, LOY is an early event in male breast carcinogenesis, which generally starts at the DCIS stage and is associated with ER and PR negative tumor