Building Interdisciplinary Learning Partnerships

Capital University Law School & Grant Medical Center partnered to provide training on medical malpractice to law students and medical residents. The course utilized a unique, interdisciplinary approach to skills training & professional development. The course attempts to meet several needs: (1) Increase experiential learning opportunities to develop professional skills and judgment; (2) Provide authentic, experiential learning that is cost-effective and accessible to all law students, including non-traditional and part­time students; and (3) Promote interdisciplinary education.https://fuse.franklin.edu/ss2016/1066/thumbnail.jp

Changes in vertical ice extent along the East Antarctic Ice Sheet margin in western Dronning Maud Land – initial field and modelling results of the MAGIC-DML collaboration

Constraining numerical ice sheet models by comparison with observational data is crucial to address the interactions between cryosphere and climate at a wide range of scales. Such models are tested and refined by comparing model predictions of past ice geometries with field-based reconstructions from geological, geomorphological, and ice core data. However, for the East Antarctic Ice sheet, there is a critical gap in the empirical data necessary to reconstruct changes in ice sheet geometry in the Dronning Maud Land (DML) region. In addition, there is poor control on the regional climate history of the ice sheet margin, because ice-core locations, where detailed reconstructions of climate history exist, are located on high inland domes. This leaves numerical models ofregional glaciation history in this near-coastal area largely unconstrained. MAGIC-DML is an ongoing Swedish-US-Norwegian-German-UK collaboration with a focus on improvingice sheet models of the western DML margin by combining advances in modeling with filling critical data gaps regarding the timing and pattern of ice-surface changes. A combination of geomorphological mapping using remote sensing data, field observations, cosmogenic nuclide surface exposure dating, and numerical ice sheetmodeling are being used in an iterative manner to produce a comprehensive reconstruction of the glacial historyof western DML. Here, we present an overview of the project, field evidence for formerly higher ice surfaces and in-situ cosmogenic nuclide measurements from the 2016/17 expedition. Preliminary field evidence indicate that interior sectors of DML have experienced a general decrease in ice sheet thickness since the late Miocene, with potential episodes of increasing thickness in the late Pleistocene (700-300 ka, 250-75 ka). To aid in interpreting these field data, new high-resolution ice sheet model reconstructions, constraining ice sheet configurations during key episodes, are presented

Mid-Pleistocene ice sheet fluctuations from cosmogenic nuclide field constraints in western Dronning Maud Land, Antarctica

The East Antarctic Ice Sheet (EAIS) is generally assumed to have been relatively insensitive to Quaternary climat echange. However, recent studies have shown potential instabilities in coastal, marine sectors of the EAIS. In addition, long-term climate reconstructions and modelling experiments indicate the potential for significant changes in ice volume and ice sheet configuration since the Pliocene. Hence, more empirical evidence for ice surface and ice volume changes is required to discriminate between contrasting inferences. MAGIC-DML is an ongoing Swedish-US-Norwegian-German-UK collaboration focused on improving ice sheetm odels by filling critical data gaps that exist in our knowledge of the timing and pattern of ice surface changes along the western Dronning Maud Land (DML) margin and combining this with advances in numerical techniques. As part of the project, field studies in the 2016/17 and 2017/18 austral summers targeted selected sites spanning accessible altitudes in the Heimefrontfjella, Vestfjella, Ahlmannryggen, Borgmassivet, and Kirwanveggen nunatakranges for in situcosmogenic nuclide sampling. Comparing concentrations of nuclides with widely differing half-lives in bedrock and erratics from a range of altitudes above modern ice surfaces can provide information on ice sheet fluctuations and complex burial and exposure histories, and thus, past configurations of non-erosive ice. Quartz-bearing rock types were sampled and analyzed for 10Be (t1/21.4 My),14C (t1/25.7 ky),26Al (t1/2705ky), and 21Ne (stable), and mafic lithologies for36Cl (t1/2301 ky). Results thus far for 3210Be and 26Al isotope pairs complemented with seven21Ne measurements have yielded some consistent patterns of paleoglaciation for the western DML margin. Eight out of fourteen bedrock samples from high-elevation (1700-2238 m a.s.l.) ridges and summits return some of the oldest exposure ages in Antarctica and have consistent 10Be,26Al, and 21Ne minimum apparent exposure ages of 1.8-4.1 Ma. Initial results therefore indicate that parts of the ice sheet marginal to the Antarctic plateau, along the Heimefrontfjella range, generally have experienced a decrease in ice thickness since the late Miocene. Another six bedrock samples (1556-1732 ma.s.l.) fall in the 300-700 ka range, and they all show significant burial. At face value, perhaps this indicates aregional ice sheet surface above 1700 m a.s.l. for much of the Plio-early Pleistocene. All other samples analyzedto date are erratics from lower elevation and more coastal sites (10 from nunataks at 553-1400 m a.s.l., and 6 froma surface moraine at 1385 m a.s.l.), exhibiting ages between 59 and 275 ka, save for two (4 and 6 ka). Whereas almost all of the nunatak erratics (including the young ones) show significant burial durations, five of the six surface moraine samples do not. These 2016/17 field samples are not yet leading to conclusive age constraints but already start to paint a picture of the western DML margin being relatively stable although there was possibly one or more episodes of relatively limited ice thickening during the last 700 ka

Fundulus as the premier teleost model in environmental biology : opportunities for new insights using genomics

Author Posting. © Elsevier B.V., 2007. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 2 (2007): 257-286, doi:10.1016/j.cbd.2007.09.001.A strong foundation of basic and applied research documents that the estuarine fish Fundulus heteroclitus and related species are unique laboratory and field models for understanding how individuals and populations interact with their environment. In this paper we summarize an extensive body of work examining the adaptive responses of Fundulus species to environmental conditions, and describe how this research has contributed importantly to our understanding of physiology, gene regulation, toxicology, and ecological and evolutionary genetics of teleosts and other vertebrates. These explorations have reached a critical juncture at which advancement is hindered by the lack of genomic resources for these species. We suggest that a more complete genomics toolbox for F. heteroclitus and related species will permit researchers to exploit the power of this model organism to rapidly advance our understanding of fundamental biological and pathological mechanisms among vertebrates, as well as ecological strategies and evolutionary processes common to all living organisms.This material is based on work supported by grants from the National Science Foundation DBI-0420504 (LJB), OCE 0308777 (DLC, RNW, BBR), BES-0553523 (AW), IBN 0236494 (BBR), IOB-0519579 (DHE), IOB-0543860 (DWT), FSML-0533189 (SC); National Institute of Health NIEHS P42-ES007381(GVC, MEH), P42-ES10356 (RTD), ES011588 (MFO); and NCRR P20 RR-016463 (DWT); Natural Sciences and Engineering Research Council of Canada Discovery (DLM, TDS, WSM) and Collaborative Research and Development Programs (DLM); NOAA/National Sea Grant NA86RG0052 (LJB), NA16RG2273 (SIK, MEH,GVC, JJS); Environmental Protection Agency U91620701 (WSB), R82902201(SC) and EPA’s Office of Research and Development (DEN)

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival