Comunidades vegetales de los bofedales de la Cordillera Real (Bolivia) bajo el calentamiento global

Los humedales altoandinos (bofedales) son uno de los más amenazados frente al calentamiento global. Dada la estrecha relación entre bofedales y agua, el retroceso glacial podrá causar su contracción. Con tres proxis del cambio climático (área de bofedales, influencia glaciar y altitud) se infirió indirectamente cómo éstos pueden afectar a las comunidades vegetales. Las hipótesis fueron 1) que la reducción del área del bofedal podría afectar negativamente la diversidad de plantas - la influencia glaciar y la altitud podrían afectar ésta relación por su efectos en la heterogeneidad ambiental y la diversidad – y 2) que el retroceso glacial puede afectar indirectamente la diversidad a través de cambios en las especies dominantes. Se midió la diversidad α y β aditiva de plantas en 20 bofedales (>4.400 m) de la Cordillera Real (Bolivia). Se encontraron 63 especies (riqueza especifica: 5-22/cuadrante 1m²). Variaciones del área e influencia glacial no se relacionaron con cambios en la vegetación, pero la diversidad fue menor a mayor altitud. Las hojas de Oxychloe andina son más resistentes al estrés ambiental por su mayor masa foliar seca, a diferencia de Distichia spp., indicando que los bofedales dominados por O. andina son más secos. Además tienen más especies que también se pueden encontrar en las laderas. Mientras, las comunidades de Distichia spp. son menos tolerantes al déficit hídrico. Si el retroceso glacial reducirá el agua en las próximas décadas, el bofedal de O. andina podrá ser más abundante a expensas de los de Distichia spp., generando desafíos para la conservación. Oxychloe andina , Retroceso glacial

Association of a single nucleotide polymorphism combination pattern of the Klotho gene with non-cardiovascular death in patients with chronic kidney disease

Chronic kidney disease (CKD) is associated with an elevated risk of all-cause mortality, with cardiovascular death being extensively investigated. However, non-cardiovascular mortality represents the biggest percentage, showing an evident increase in recent years. Klotho is a gene highly expressed in the kidney, with a clear influence on lifespan. Low levels of Klotho have been linked to CKD progression and adverse outcomes. Single nucleotide polymorphisms (SNPs) of the Klotho gene have been associated with several diseases, but studies investigating the association of Klotho SNPs with noncardiovascular death in CKD populations are lacking. The main aim of this study was to assess whether 11 Klotho SNPs were associated with non-cardiovascular death in a subpopulation of the National Observatory of Atherosclerosis in Nephrology (NEFRONA) study (n ¼ 2185 CKD patients). After 48 months of follow-up, 62 cardiovascular deaths and 108 non-cardiovascular deaths were recorded. We identified a high non-cardiovascular death risk combination of SNPs corresponding to individuals carrying the most frequent allele (G) at rs562020, the rare allele (C) at rs2283368 and homozygotes for the rare allele (G) at rs2320762 (rs562020 GG/AG þ rs2283368 CC/CT þ rs2320762 GG). Among the patients with the three SNPs genotyped (n ¼ 1016), 75 (7.4%) showed this combination. Furthermore, 95 (9.3%) patients showed a low-risk combination carrying all the opposite genotypes (rs562020 AA þ rs2283368 TT þ rs2320762 GT/TT). All the other combinations [n ¼ 846 (83.3%)] were considered as normal risk. Using competing risk regression analysis, we confirmed that the proposed combinations are independently associated with a higher fhazard ratio [HR] 3.28 [confidence interval (CI) 1.51-7.12]g and lower [HR 6 × 10- (95% CI 3.3 × 10--1.1 × 10-)] risk of suffering a non-cardiovascular death in the CKD population of the NEFRONA cohort compared with patients with the normal-risk combination. Determination of three SNPs of the Klotho gene could help in the prediction of non-cardiovascular death in CKD

Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine

[This corrects the article DOI: 10.1186/s13054-016-1208-6.]

Canagliflozin and Renal Outcomes in Type 2 Diabetes and Nephropathy

BACKGROUND Type 2 diabetes mellitus is the leading cause of kidney failure worldwide, but few effective long-term treatments are available. In cardiovascular trials of inhibitors of sodium–glucose cotransporter 2 (SGLT2), exploratory results have suggested that such drugs may improve renal outcomes in patients with type 2 diabetes. METHODS In this double-blind, randomized trial, we assigned patients with type 2 diabetes and albuminuric chronic kidney disease to receive canagliflozin, an oral SGLT2 inhibitor, at a dose of 100 mg daily or placebo. All the patients had an estimated glomerular filtration rate (GFR) of 30 to 300 to 5000) and were treated with renin–angiotensin system blockade. The primary outcome was a composite of end-stage kidney disease (dialysis, transplantation, or a sustained estimated GFR of <15 ml per minute per 1.73 m 2), a doubling of the serum creatinine level, or death from renal or cardiovascular causes. Prespecified secondary outcomes were tested hierarchically. RESULTS The trial was stopped early after a planned interim analysis on the recommendation of the data and safety monitoring committee. At that time, 4401 patients had undergone randomization, with a median follow-up of 2.62 years. The relative risk of the primary outcome was 30% lower in the canagliflozin group than in the placebo group, with event rates of 43.2 and 61.2 per 1000 patient-years, respectively (hazard ratio, 0.70; 95% confidence interval [CI], 0.59 to 0.82; P=0.00001). The relative risk of the renal-specific composite of end-stage kidney disease, a doubling of the creatinine level, or death from renal causes was lower by 34% (hazard ratio, 0.66; 95% CI, 0.53 to 0.81; P<0.001), and the relative risk of end-stage kidney disease was lower by 32% (hazard ratio, 0.68; 95% CI, 0.54 to 0.86; P=0.002). The canagliflozin group also had a lower risk of cardiovascular death, myocardial infarction, or stroke (hazard ratio, 0.80; 95% CI, 0.67 to 0.95; P=0.01) and hospitalization for heart failure (hazard ratio, 0.61; 95% CI, 0.47 to 0.80; P<0.001). There were no significant differences in rates of amputation or fracture. CONCLUSIONS In patients with type 2 diabetes and kidney disease, the risk of kidney failure and cardiovascular events was lower in the canagliflozin group than in the placebo group at a median follow-up of 2.62 years

Canagliflozin and renal outcomes in type 2 diabetes and nephropathy

BACKGROUND Type 2 diabetes mellitus is the leading cause of kidney failure worldwide, but few effective long-term treatments are available. In cardiovascular trials of inhibitors of sodium–glucose cotransporter 2 (SGLT2), exploratory results have suggested that such drugs may improve renal outcomes in patients with type 2 diabetes. METHODS In this double-blind, randomized trial, we assigned patients with type 2 diabetes and albuminuric chronic kidney disease to receive canagliflozin, an oral SGLT2 inhibitor, at a dose of 100 mg daily or placebo. All the patients had an estimated glomerular filtration rate (GFR) of 30 to &lt;90 ml per minute per 1.73 m2 of body-surface area and albuminuria (ratio of albumin [mg] to creatinine [g], &gt;300 to 5000) and were treated with renin–angiotensin system blockade. The primary outcome was a composite of end-stage kidney disease (dialysis, transplantation, or a sustained estimated GFR of &lt;15 ml per minute per 1.73 m2), a doubling of the serum creatinine level, or death from renal or cardiovascular causes. Prespecified secondary outcomes were tested hierarchically. RESULTS The trial was stopped early after a planned interim analysis on the recommendation of the data and safety monitoring committee. At that time, 4401 patients had undergone randomization, with a median follow-up of 2.62 years. The relative risk of the primary outcome was 30% lower in the canagliflozin group than in the placebo group, with event rates of 43.2 and 61.2 per 1000 patient-years, respectively (hazard ratio, 0.70; 95% confidence interval [CI], 0.59 to 0.82; P=0.00001). The relative risk of the renal-specific composite of end-stage kidney disease, a doubling of the creatinine level, or death from renal causes was lower by 34% (hazard ratio, 0.66; 95% CI, 0.53 to 0.81; P&lt;0.001), and the relative risk of end-stage kidney disease was lower by 32% (hazard ratio, 0.68; 95% CI, 0.54 to 0.86; P=0.002). The canagliflozin group also had a lower risk of cardiovascular death, myocardial infarction, or stroke (hazard ratio, 0.80; 95% CI, 0.67 to 0.95; P=0.01) and hospitalization for heart failure (hazard ratio, 0.61; 95% CI, 0.47 to 0.80; P&lt;0.001). There were no significant differences in rates of amputation or fracture. CONCLUSIONS In patients with type 2 diabetes and kidney disease, the risk of kidney failure and cardiovascular events was lower in the canagliflozin group than in the placebo group at a median follow-up of 2.62 years

Nurses' perceptions of aids and obstacles to the provision of optimal end of life care in ICU

Contains fulltext : 172380.pdf (publisher's version ) (Open Access

Coryphantha spp. proximate composition and phytochemical profile

Mexico has an important diversity of cacti. Coryphantha species are abundant in Lagos de Moreno, located in the highlands of Mexico, in the state of Jalisco. However, little is known about their chemical composition. In this research work, three Coryphantha species (Coryphantha bumamma, C. clavata and C. cornifera) were studied to determine their chemical composition of primary metabolites (proximate analysis) and to identify the presence or absence of some secondary metabolites (phytochemical profile). Results showed a higher concentration for moist, ash, fat and fiber in C. bumamma and C. clavata. Coryphantha clavata and C. cornifera presented higher content of protein and carbohydrates. Alkaloids, saponins, flavonoids and sterols (triterpenes) were detected in all three species; while tannins and quinones were absent in all samples. The results were compared to chemical information reported for other Cactaceae. The information here presented is valuable in order to establish a database for the potential use of these species as phytochemical source

Coryphantha spp. proximate composition and phytochemical profile

Mexico has an important diversity of cacti. Coryphantha species are abundant in Lagos de Moreno, located in the highlands of Mexico, in the state of Jalisco. However, little is known about their chemical composition. In this research work, three Coryphantha species (Coryphantha bumamma, C. clavata and C. cornifera) were studied to determine their chemical composition of primary metabolites (proximate analysis) and to identify the presence or absence of some secondary metabolites (phytochemical profile). Results showed a higher concentration for moist, ash, fat and fiber in C. bumamma and C. clavata. Coryphantha clavata and C. cornifera presented higher content of protein and carbohydrates. Alkaloids, saponins, flavonoids and sterols (triterpenes) were detected in all three species; while tannins and quinones were absent in all samples. The results were compared to chemical information reported for other Cactaceae. The information here presented is valuable in order to establish a database for the potential use of these species as phytochemical source

Effects of indirect facilitation on functional diversity, dominance and niche differentiation in tropical alpine communities

International audienceQuestion: Positive interactions between plants have well-known effects on community species richness and biomass via micro-environment improvements. However, the effects of indirect facilitation performed by a nurse on an associated plant community, occurring e.g., through protection against grazing, have not attracted much attention in the literature so far, in particular regarding the functional traits of the associated plant communities. Because characteristics of trait distributions can reveal selective pressures at play, they offer valuable information for the study of grazing pressure. Here, we tested the extent to which indirect facilitation affects the amount of distinct trait combinations (H1), trait dominance (H2) and niche differentiation between species (H3) of associated plant communities living under nurse plants. Location: Tropical alpine peatlands, Cordillera Real, Bolivia. Methods: We set up a grazing exclusion experiment over two dominant nurse cushion species: one providing indirect facilitation under grazing pressure and the other not. We measured three functional traits in the associated communities, which are known to vary depending on the grazing level: LDMC, leaf thickness and maximum height. We assessed the amount of distinct trait combinations by computing the volume of the phenotypic space (H1). The variation in trait dominance was quantified with kurtosis and skewness of the trait distributions (H2). The variation in niche differentiation was evaluated using mean intra-population trait variance relative to intra-community trait variance (H3). Results: We did not find a significant effect of grazing and indirect facilitation on the volume of the phenotypic space (H1). However, our study revealed a significant effect of indirect facilitation on dominance in the associated communities by maintaining the evenness of the trait distributions (maximum height and leaf thickness) in grazed compared to ungrazed plots (H2), and on niche differentiation by maintaining trait overlaps (LDMC and maximum height) between species in grazed compared to ungrazed contexts (H3). Conclusion: Our results suggest that indirect facilitation promotes the co-existence of contrasting functional strategies (H2) and species niches (H3) in associated plant communities subject to grazing. These results reflect the buffering effect of indirect facilitation on the grazing selective pressures exerted on associated plant communities living under the protection of nurses