An ex vivo model using human osteoarthritic cartilage demonstrates the release of bioactive insulin-like growth factor-1 from a collagen-glycosaminoglycan scaffold.

Biomimetic scaffolds hold great promise for therapeutic repair of cartilage, but although most scaffolds are tested with cells in vitro, there are very few ex vivo models (EVMs) where adult cartilage and scaffolds are co-cultured to optimize their interaction prior to in vivo studies. This study describes a simple, non-compressive method that is applicable to mammalian or human cartilage and provides a reasonable throughput of samples. Rings of full-depth articular cartilage slices were derived from human donors undergoing knee replacement for osteoarthritis and a 3 mm core of a collagen/glycosaminoglycan biomimetic scaffold (Tigenix, UK) inserted to create the EVM. Adult osteoarthritis chondrocytes were seeded into the scaffold and cultures maintained for up to 30 days. Ex vivo models were stable throughout experiments, and cells remained viable. Chondrocytes seeded into the EVM attached throughout the scaffold and in contact with the cartilage explants. Cell migration and deposition of extracellular matrix proteins in the scaffold was enhanced by growth factors particularly if the scaffold was preloaded with growth factors. This study demonstrates that the EVM represents a suitable model that has potential for testing a range of therapeutic parameters such as numbers/types of cell, growth factors or therapeutic drugs before progressing to costly pre-clinical trials.The authors would like to kindly acknowledge funding from the EPSRC and Tigenix Ltd (LM), Technology Strategy Board and Tigenix Ltd (JW) and the NIHR (DH).This is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1002/cbf.3112

Erratum: The helium abundance in the ejecta of U Scorpii

U Scorpii (U Sco) is a recurrent nova which has been observed in outburst on 10 occasions, most recently in 2010. We present near-infrared (near-IR) and optical spectroscopy of the 2010 outburst of U Sco. The reddening of U Sco is found to be E(B − V) = 0.14 ± 0.12, consistent with previous determinations, from simultaneous optical and near-IR observations. The spectra show the evolution of the linewidths and profiles to be consistent with previous outbursts. Velocities are found to be up to 14 000 km s−1 in broad components and up to 1800 km s−1 in narrow-line components, which become visible around day 8 due to changes in the optical depth. From the spectra we derive a helium abundance of N(He)/N(H) = 0.073 ± 0.031 from the most reliable lines available; this is lower than most other estimates and indicates that the secondary is not helium-rich, as previous studies have suggested

Physical prognostic factors predicting outcome following anterior cruciate ligament reconstruction: Protocol for a systematic review

Introduction Injuries of the anterior cruciate ligament (ACL) are a common musculoskeletal complication and can cause significant reduction in patient function and quality of life. Many undergo ACL reconstruction, with high-quality rehabilitation key to successful outcome. Knowledge of physical prognostic factors, such as quadriceps strength, is crucial to inform rehabilitation and has important implications for outcome following ACL reconstruction. However, these factors predicting outcome are poorly defined. Therefore, the aim of this systematic review is to establish physical prognostic factors predictive of outcome in adults following ACL reconstruction. Outcome will be subdivided into two groups of outcome measures, patient-reported and performance-based. Physical prognostic factors of interest will reflect a range of domains and may be modifiable/non-modifiable. Results will help decide most appropriate management and assist in planning and tailoring preoperative and postoperative rehabilitation. Methods and analysis This systematic review protocol is reported according to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols. MEDLINE, CINAHL and EMBASE databases, key journals and grey literature will be searched from inception to July 2019. Prospective cohort studies including participants aged ≥16 years who have undergone ACL reconstruction will be included, with articles focusing on multi-ligament reconstructions and ACL repair surgery, or not published in English excluded. Two independent reviewers will conduct searches, assess study eligibility, extract data, assess risk of bias (Quality in Prognostic Studies tool) and quantify overall quality of evidence (modified Grading of Recommendations, Assessment, Development and Evaluation guidelines). If possible, a meta-analysis will be conducted, otherwise a narrative synthesis will ensue focusing on prognostic factors, risk of bias of included studies and strength of association with outcomes. Ethics and dissemination Findings will be published in a peer-reviewed journal, presented at conferences and locally to physiotherapy departments. Ethical approval is not required for this systematic review. PROSPERO registration number CRD42019127732

Impact of foot-and-mouth disease on mastitis and culling on a large-scale dairy farm in Kenya

Foot and mouth disease (FMD) is a highly transmissible viral infection of cloven hooved animals associated with severe economic losses when introduced into FMD-free countries. Information on the impact of the disease in FMDV-endemic countries is poorly characterised yet essential for the prioritisation of scarce resources for disease control programmes. A FMD (virus serotype SAT2) outbreak on a large-scale dairy farm in Nakuru County, Kenya provided an opportunity to evaluate the impact of FMD on clinical mastitis and culling rate. A cohort approach followed animals over a 12-month period after the commencement of the outbreak. For culling, all animals were included; for mastitis, those over 18 months of age. FMD was recorded in 400/644 cattle over a 29-day period. During the follow-up period 76 animals were culled or died whilst in the over 18 month old cohort 63 developed clinical mastitis. Hazard ratios (HR) were generated using Cox regression accounting for non-proportional hazards by inclusion of time-varying effects. Univariable analysis showed FMD cases were culled sooner but there was no effect on clinical mastitis. After adjusting for possible confounders and inclusion of time-varying effects there was weak evidence to support an effect of FMD on culling (HR = 1.7, 95% confidence intervals [CI] 0.88-3.1, P = 0.12). For mastitis, there was stronger evidence of an increased rate in the first month after the onset of the outbreak (HR = 2.9, 95%CI 0.97-8.9, P = 0.057)

Near-infrared studies of the 2010 outburst of the recurrent nova U Scorpii

We present near-infrared (near-IR) observations of the 2010 outburst of U Sco. JHK photometry is presented on 10 consecutive days starting from 0.59 d after outburst. Such photometry can gainfully be integrated into a larger data base of other multiwavelength data which aim to comprehensively study the evolution of U Sco. Early near-IR spectra, starting from 0.56 d after outburst, are presented and their general characteristics discussed. Early in the eruption, we see very broad wings in several spectral lines, with tails extending up to ∼10 000 km s−1 along the line of sight; it is unexpected to have a nova with ejection velocities equal to those usually thought to be exclusive to supernovae. From recombination analysis, we estimate an upper limit of [inline image] for the ejected mass

Double radio peak and non-thermal collimated ejecta in RS Ophiuchi following the 2006 outburst

We report Multi-Element Radio-Linked Interferometer Network, Very Large Array, One-Centimetre Radio Array, Very Long Baseline Array (VLBA), Effelsberg and Giant Metrewave Radio Telescope observations beginning 4.5 days after the discovery of RS Ophiuchi undergoing its 2006 recurrent nova outburst. Observations over the first 9 weeks are included, enabling us to follow spectral development throughout the three phases of the remnant development. We see dramatic brightening on days 4 to 7 at 6 GHz and an accompanying increase in other bands, particularly 1.46 GHz, consistent with transition from the initial ‘free expansion’ phase to the adiabatic expansion phase. This is complete by day 13 when the flux density at 5 GHz is apparently declining from an unexpectedly early maximum (compared with expectations from observations of the 1985 outburst). The flux density recovered to a second peak by approximately day 40, consistent with behaviour observed in 1985. At all times the spectral index is consistent with mixed non-thermal and thermal emission. The spectral indices are consistent with a non-thermal component at lower frequencies on all dates, and the spectral index changes show that the two components are clearly variable. The estimated extent of the emission at 22 GHz on day 59 is consistent with the extended east and west features seen at 1.7 GHz with the VLBA on day 63 being entirely non-thermal. We suggest a two-component model, consisting of a decelerating shell seen in mixed thermal and non-thermal emission plus faster bipolar ejecta generating the non-thermal emission, as seen in contemporaneous VLBA observations. Our estimated ejecta mass of 4 ± 2 × 10−7 M⊙ is consistent with a white dwarf (WD) mass of 1.4 M⊙. It may be that this ejecta mass estimate is a lower limit, in which case a lower WD mass would be consistent with the data

He abundance in the ejecta of U Sco (Erratum)

The paper ‘The helium abundance in the ejecta of U Scorpi’ was published in MNRAS, 419, 1465 (2012).An error has been identified in the abundance calculations, including the error analysis

Quality of wheelchair services as perceived by users in rural Bangalore district, India: a cross-sectional survey

Background and aim This study investigated the current state of wheelchair services in Bangalore Rural district, as provided by Bangalore Baptist Hospital, and identified areas for improvement. Method a cross-sectional survey was held among 50 wheelchair users. Data was collected on demographics, satisfaction, wheelchair skills and level of disability using QUEST, WST-Q and CHART-SF questionnaires. Result Overall satisfaction can be described as more or less satisfied, scoring 3.8 out of 5. Wheelchair users were less satisfied with the services compared to the wheelchair itself. The skills a wheelchair users had were strongly correlated with satisfaction scores (p < 0.01). Differences in satisfaction between genders were observed and related to multiple factors. Conclusion Wheelchairs should be easy to use with support services being easily accessible. A wheelchair should be delivered together with a training program to provide the user with the skills to operate and maintain the wheelchair. There are gender-wise differences in satisfaction towards wheelchair services that influence satisfaction Implications for rehabilitation Training of wheelchair skills should be part of the delivery process as this positively impacts satisfaction of wheelchair users and increases their independent mobility. Requirements towards a wheelchair and its support service are gender specific. Recognizing and acting upon the differing needs between male and female wheelchair users should be an integral part of a wheelchair provision service. Wheelchair users should take part in the development and implementation of novel technologies, policies and service delivery schemes as their need is not always answered. This could lead to a reduced uptake, or even failure, of innovations in (wheelchair) services. The financial situation of a wheelchair user is a factor that influences satisfaction. Therefore, empowering and/or enabling wheelchair users to take part in activities that earns them an income should be part of a wheelchair service