A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus \u3ci\u3eMagnaporthe oryzae\u3c/i\u3e within rice cells

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these effects, while glucose addition only suppresses host defenses. Our results indicate that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression

A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus \u3ci\u3eMagnaporthe oryzae\u3c/i\u3e within rice cells

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these effects, while glucose addition only suppresses host defenses. Our results indicate that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression

A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus \u3ci\u3eMagnaporthe oryzae\u3c/i\u3e within rice cells

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these effects, while glucose addition only suppresses host defenses. Our results indicate that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression

Costos de la aplicación de diferentes productos para el control de garrapata (Rhipicephalus microplus) en ganado vacuno

The objective of this work was to compare the costs of the application of acaricides used in the control of Rhipicephalus microplus ticks, considering the necessary inputs for the application, the personnel involved and the indirect expenses generated in each treatment. The methodology consisted of applying different products Cypermethrin®, Azuntol®, Fluazuron®, Ivermectin®, and a parasitic fungus (Metarhizium anisopliae) to control the tick and identify the costs of the application. The highest cost per year of treatment per head of cattle corresponded to Fluazuron® with an amount of $1,282.50 per head of cattle, secondly Ivermectin® with a total of$1,053.45, followed by Metarhizium anisopliae with the amount of $279.00, Azuntol® with a total of$162.85 and finally Cypermethrin® $109.93. Although the use of the parasitic fungus was not the most economical, it offers environmental advantages over the other products.El presente trabajo tuvo como objetivo realizar la comparación de costos de la aplicación de acaricidas usados en el control de garrapata Rhipicephalus microplus, considerando los insumos necesarios para la aplicación, el personal que interviene y los gastos indirectos generados en cada tratamiento. La metodología consistió en aplicar diferentes productos Cipermetrina®, Azuntol®, Fluazurón®, Ivermectina®, y un hongo parasitario (Metarhizium anisopliae) para controlar la garrapata e identificar los costos de la aplicación. El mayor costo por año de tratamiento por cabeza de ganado correspondió al Fluazuron® con un importe de$1,282.50 por cabeza de ganado, en segundo lugar la Ivermectina® con un total de $1,053.45, seguido por Metarhizium anisopliae con la cantidad de$279.00, Azuntol® con un total de $162.85 y finalmente Cipermetrina®$109.93. A pesar de que el uso del hongo parasitario no fue el más económico, este ofrece ventajas ambientales sobre los demás productos

Enhancing Sustainable Mobility: Multi-Criteria Analysis for Electric Vehicle Integration and Policy Implementation

In contemporary times, regulations have been established to govern access and integration of electric vehicles, which hold immense potential in mitigating the adverse impacts of transportation arising from escalating mobility demands and rapid urbanization. Nevertheless, their implementation poses intricate challenges due to the multifaceted nature of sustainability and other complexities, as they are considered a prospective solution to address the pressing issue of climate change and achieve holistic sustainability.  Moreover, this study elucidates the methodological approach employed for selecting a comprehensive set of criteria to be considered in energy planning initiatives involving the incorporation of electric vehicles, encompassing both renewable and conventional energy sources. The selection process was informed through a thorough review of existing literature and the insightful input from experts, utilizing the Likert scale and arithmetic mean as reference benchmarks. The outcomes of this research have yielded the identification of 27 sub-criteria, categorized into five distinct groups: technical, economic, social, environmental, and risk-related factors, thereby enabling a comprehensive evaluation of energy planning projects associated with the adoption of electric vehicles. The findings derived from employing the hierarchical analytical process with fuzzy logic FAHP highlight the considerable potential of most criteria and sub-criteria in devising effective measures. Notably, criteria related to policy-governance and environmental aspects emerged as the most influential, according to the collective expertise of the consulted group. This study significantly contributes to enhancing sustainable mobility and advancing progress towards attaining sustainable development goals. By delineating pertinent criteria and sub-criteria for the planning process, this research augments the literature dedicated to supporting informed decision-making in sustainable transportation, particularly through the potential application of a Multi-Criteria Decision Analysis (MCDA) method

The physical properties of star forming galaxies in the low redshift universe

(modified) We present a comprehensive study of the physical properties of \~10^5 galaxies with measurable star formation in the SDSS. By comparing physical information extracted from the emission lines with continuum properties, we build up a picture of the nature of star-forming galaxies at z<0.2. We take out essentially all aperture bias using resolved imaging, allowing an accurate estimate of the total SFRs in galaxies. We determine the SFR density to be 1.915^{+0.02}_{-0.01}(rand.)^{+0.14}_{-0.42} (sys.) h70 10^{-2} Msun/yr/Mpc^3 at z=0.1 (for a Kroupa IMF) and we study the distribution of star formation as a function of various physical parameters. The majority of the star formation in the low redshift universe takes place in moderately massive galaxies (10^10-10^11 Msun), typically in HSB disk galaxies. Roughly 15% of all star formation takes place in galaxies that show some sign of an active nucleus. About 20% occurs in starburst galaxies. We show that the present to past-average star formation rate, the Scalo b-parameter; is almost constant over almost three orders of magnitude in mass, declining only at M*>10^10 Msun. The volume averaged b parameter is 0.408^{+0.005}_{-0.002} (rand).^{+0.029}_{-0.090} (sys.) h70^{-1}. We use this value constrain the star formation history of the universe. In agreement with other work we find a correlation between $b$ and morphological type, as well as a tight correlation between the 4000AA break (D4000) and b. We discuss how D4000 can be used to estimate b parameters for high redshift galaxies.Comment: Accepted for MNRAS. Replaced with accepted version. A section on comparison with other methods of SFR estimation added and various updates have been made. The main results are almost unchange

Diagnostic and Therapeutic Problems of Pulmonary Tuberculosis in Elderly Patients

To identify differences in the clinical, radiologic, and microbiologic features of pulmonary tuberculosis (TB) in the young (<64 yr) and elderly (≥65 yr), we performed a retrospective analysis of the medical charts and chest radiographs of 207 young and 119 elderly pulmonary TB patients. Hemoptysis and a febrile sense were more frequent in the young, whereas weakness, dyspnea, anorexia, and mental change were more frequent in the elderly. Elderly patients showed higher frequencies of cardiovascular and chronic lung diseases, whereas the young showed a higher proportion of underlying liver disease. In addition, chest radiography showed a significantly higher frequency of mid or lower lung involvement by TB lesions in the elderly (10.6% vs. 22.7%, p<0.05). Lesions were frequently misdiagnosed as pneumonia or lung cancer in the elderly. However, there was no difference between these two groups in terms of sputum acid-fast bacilli positivity. The elderly showed a higher frequency of adverse drug reactions (18.5% vs. 40.7%, p<0.05), and higher TB-related mortality (1.3% vs. 11.1%, p<0.05). In conclusion this study showed that young and elderly pulmonary TB patients have similar microbiologic features; however, the elderly showed higher frequencies of atypical clinical and radiologic presentations, adverse drug reactions, and higher TB-related mortality

Chronic flavanol-rich cocoa powder supplementation reduces body fat mass in endurance athletes by modifying the follistatin/ myostatin ratio and leptin levels

Flavonols-rich cocoa has positive effects on lipid metabolism and might enhance the performance of athletes through an improvement in their body composition. To test this hypothesis a placebo-controlled intervention study in training endurance athletes who received 5 g of cocoa daily (425 mg of flavan-3-ols) for 10 weeks was performed. Dietary intake, body composition, exercise performance and plasma levels of follistatin, myostatin and leptin were measured. Cocoa intake significantly reduced body fat percentage (p = 0.020), specifically in the trunk (p = 0.022), visceral area (p = 0.034) and lower limbs (p = 0.004). The reduction in body fat mass was accompanied by an increase in plasma follistatin and a decrease in leptin, while myostatin levels remained unchanged. The intake of cocoa reduced the percentage of body fat of athletes, without any impact on athletes' performance. The change in fat body composition did not improve athletes' performance

Antioxidant activity of fractions from Quercus sideroxyla bark and identifi cation of proanthocyanidins by HPLC-DAD and HPLC-MS

The most active phenolics in Quercus sideroxyla Humb. & Bonpl. residual bark were identified and evaluated following a chromatographic fractionation. Bark powder was defatted with hexane and crude extract (CE) was obtained by extraction with aqueous acetone (70%). A liquid partition with ethyl acetate was performed to produce an organic extract (OE), which was subsequently purified by column chromatography (Toyopearl HW-40F, methanol), and resulted in six methanolic fractions (MF1 to MF6) and an oligomeric fraction (OLF) eluted with acetone 67%. Extraction yields, total phenolic and flavanol contents were determined. The antioxidant activity of bark extracts was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox (6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic-acid)-equivalent antioxidant capacity (TEAC), and ferric ion reducing antioxidant power (FRAP) assays. Their median effective concentration (EC₅₀) data and rate constants for DPPH radical scavenging were also estimated. Identification of major phenolics was carried out by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography with electrospray ionization coupled to mass spectrometry (HPLC-ESI-MS) instruments. Bioactive gallic acid, catechin, epicatechin, gallocatechin, catechin gallate, dimeric procyanidins, galloylated dimeric proanthocyanidins, trimeric procyanidins, and tetrameric proanthocyanidins were detected and identified in Q. sideroxyla bark extracts. MF2 was the most active fraction containing gallocatechin as its major compound; MF5 and OLF contain galloylated procyanidins, which may explain their higher antiradical activity. OLF besides galloylated procyanidins has gallocatechin, which presumably contributes to its higher antiradical activity. Consequently, Q. sideroxyla bark could be a good source of therapeutic health products or nutraceutical ingredients that may exert a potential prevention or treatment action against diseases in biological systems.This is the publisher’s final pdf. The published article is copyrighted by Walter de Gruyter and can be found at: http://www.degruyter.com/view/j/hfsg.Keywords: proanthocyanidins, Quercus sideroxyla ., bark, antiradical activity, HPL