Espécies de Belostoma Latreille (Heteroptera, Belostomatidae) de pequeno porte. III. Revisão do grupo oxyurum com uma nova espécie do Brasil e a descrição do macho de B. noualhieri Montandon

Species of the oxyurum group (sensu Lauck) consist of five extant Neotropical small species, whose lengths range 15.0 to 20.0 mm. The anterior interocular width about 1.5 times the width of an eye and ventral diverticulum of phallus flattened, circular, and large are, in combination, diagnostic. The small species of the oxyurum group were included in the Lauck's key to the identification of the species groups, without dealing with the species included in it because many of them are very similar in appearance. Therefore here we redescribe and key the Belostoma species of the oxyurum group. Belostoma oxyurum (Dufour) is newly recorded from Brazil (Paraná and Rio Grande do Sul). Holotype and lectotype are designated for B. oxyurum and B. sanctulum Montandon, respectively. The aspect of the prosternal keel, the ratio between the width of the ventral diverticulum of phallus and its length in ventral view, and the aspect of dorsal arms of ventral diverticulum have proven useful for better species delimitation. Based on specimens from Pará State (N. Brazil), Belostoma carajaensis Ribeiro & Estévez, sp. nov. is described and illustrated. This new species differs from B. sanctulum in having anteoculus shorter than interoculus and the dorsal arms of ventral diverticulum divergent and large. A male specimen of B. noualhieri Montandon was collected in São Paulo State and based mainly on features of male genitalia, this species is here also included under oxyurum group. © 2009 Sociedade Brasileira De Entomologia.Representantes do grupo oxyurum (sensu Lauck) compreendem cinco espécies neotropicais de pequeno porte, com comprimento total do corpo entre 15,0 e 20,0 mm. A largura interocular anterior cerca de uma vez e meia a largura de um olho e os formatos achatado, circular e alargado do divertículo ventral do falossoma são característicos de seus representantes. Esse grupo de espécies foi incluído na chave de identificação de Lauck para grupos de espécies de Belostoma Latreille, mas seus representantes não tiveram sua taxonomia detalhada por serem espécies com proximidade morfológica grande. Neste trabalho, as espécies do grupo oxyurum são redescritas e uma chave de identificação para suas espécies é fornecida. É registrada pela primeira vez no Brasil a espécie B. oxyurum (Dufour) nos estados do Paraná e do Rio Grande do Sul. Um holótipo e um lectótipo foram designados para B. oxyurum e B. sanctulum Montandon respectivamente. O aspecto da carena prosternal, a relação entre a largura do divertículo ventral do falossoma e seu comprimento em vista ventral e o aspecto dos braços dorsais do divertículo ventral mostraram-se importantes na melhor definição das espécies do grupo. Belostoma carajaensis Ribeiro & Estévez, sp. nov. é descrita e ilustrada com base em espécimes do Estado do Pará, norte do Brasil e se diferencia de B. sanctulum pelo comprimento do anteóculo menor que o do interóculo e pelos braços dorsais do divertículo ventral divergentes e largos. Um espécime macho de B. noualhieri Montandon foi coligido no Estado de São Paulo e, baseado principalmente nas características observadas da genitália masculina deles, essa espécie é aqui incluída no grupo oxyurum.Facultad de Ciencias Naturales y Muse

Espécies de Belostoma Latreille (Heteroptera, Belostomatidae) de pequeno porte. III. Revisão do grupo oxyurum com uma nova espécie do Brasil e a descrição do macho de B. noualhieri Montandon

Species of the oxyurum group (sensu Lauck) consist of five extant Neotropical small species, whose lengths range 15.0 to 20.0 mm. The anterior interocular width about 1.5 times the width of an eye and ventral diverticulum of phallus flattened, circular, and large are, in combination, diagnostic. The small species of the oxyurum group were included in the Lauck's key to the identification of the species groups, without dealing with the species included in it because many of them are very similar in appearance. Therefore here we redescribe and key the Belostoma species of the oxyurum group. Belostoma oxyurum (Dufour) is newly recorded from Brazil (Paraná and Rio Grande do Sul). Holotype and lectotype are designated for B. oxyurum and B. sanctulum Montandon, respectively. The aspect of the prosternal keel, the ratio between the width of the ventral diverticulum of phallus and its length in ventral view, and the aspect of dorsal arms of ventral diverticulum have proven useful for better species delimitation. Based on specimens from Pará State (N. Brazil), Belostoma carajaensis Ribeiro & Estévez, sp. nov. is described and illustrated. This new species differs from B. sanctulum in having anteoculus shorter than interoculus and the dorsal arms of ventral diverticulum divergent and large. A male specimen of B. noualhieri Montandon was collected in São Paulo State and based mainly on features of male genitalia, this species is here also included under oxyurum group. © 2009 Sociedade Brasileira De Entomologia.Representantes do grupo oxyurum (sensu Lauck) compreendem cinco espécies neotropicais de pequeno porte, com comprimento total do corpo entre 15,0 e 20,0 mm. A largura interocular anterior cerca de uma vez e meia a largura de um olho e os formatos achatado, circular e alargado do divertículo ventral do falossoma são característicos de seus representantes. Esse grupo de espécies foi incluído na chave de identificação de Lauck para grupos de espécies de Belostoma Latreille, mas seus representantes não tiveram sua taxonomia detalhada por serem espécies com proximidade morfológica grande. Neste trabalho, as espécies do grupo oxyurum são redescritas e uma chave de identificação para suas espécies é fornecida. É registrada pela primeira vez no Brasil a espécie B. oxyurum (Dufour) nos estados do Paraná e do Rio Grande do Sul. Um holótipo e um lectótipo foram designados para B. oxyurum e B. sanctulum Montandon respectivamente. O aspecto da carena prosternal, a relação entre a largura do divertículo ventral do falossoma e seu comprimento em vista ventral e o aspecto dos braços dorsais do divertículo ventral mostraram-se importantes na melhor definição das espécies do grupo. Belostoma carajaensis Ribeiro & Estévez, sp. nov. é descrita e ilustrada com base em espécimes do Estado do Pará, norte do Brasil e se diferencia de B. sanctulum pelo comprimento do anteóculo menor que o do interóculo e pelos braços dorsais do divertículo ventral divergentes e largos. Um espécime macho de B. noualhieri Montandon foi coligido no Estado de São Paulo e, baseado principalmente nas características observadas da genitália masculina deles, essa espécie é aqui incluída no grupo oxyurum.Facultad de Ciencias Naturales y Muse

First in situ observations of soft bottom megafauna from the Cascais Canyon head

We report the first in situ observations of soft bottom megafauna from the Cascais Canyon head. Observations were collected opportunistically during three technical dives with the ROV Luso between 460-805 m at two locations distanced 1,230 m. The habitats were clas-sified as upper bathyal fine mud. The soft bottom fauna was dominated by burrows of Nephrops norvegicus reaching up to 2.9 burrows/m2, a common habitat along the Portu-guese continental margin. To our knowledge, densities are the highest ever reported for depths below 300 m. The ichthyofauna at the upper Cascais Canyon is a mixture of lower shelf and upper bathyal species, including Phycis blennoides, Scyliorhynus canicula, Coe-lorhynchus labiatus/occa and Chimaera monstrosa. Bait release attracted Myxine glutinosa. Surveys in other geological settings of the Cascays Canyon are required to understand more comprehensively the diversity of its sessile and vagile biodiversity

Espécies de Heterópteros Dulciaquícolas (Hemiptera, Heteroptera, Gerromorpha e Nepomorpha) Registradas no Estado do Rio de Janeiro, Brasil

A preliminary list of the species of Gerromorpha and Nepomorpha from Rio de Janeiro State, southeastern Brazil, is presented. The list, which was prepared with basis on a careful survey of the literature and examination collections, includes 37 semiaquatic and 76 water bug species, totalizing 113 recorded species. The available data on the distribution of each species within the state (municipalities) are given. Most of the recorded species are from the now heavily fragmented Atlantic Forest of Brazil.Uma lista preliminar das espécies de Gerromorpha e Nepomorpha no Estado do Rio de Janeiro, sudeste do Brasil, é apresentada. A lista, preparada a partir de cuidadoso estudo da literatura e exame de coleções, inclui 113 espécies de percevejos dulciaquícolas, 37 de gerromorfos e 76 de nepomorfos. As informações disponíveis sobre a distribuição de cada espécie no estado (municípios) são fornecidas. A maior parte das espécies registradas é proveniente da Mata Atlântica brasileira, que hoje encontra-se muito fragmentada

Antioxidant compounds recovery from Juçara residue by thermal assisted extraction

This study aimed to recover bioactive compounds by solid-liquid extraction from the agro-industrial residue obtained during juçara fruits processing into pulp. A preliminary study using different solvents (methanol, ethanol and water) indicated ethanol in aqueous solution as the best solvent for antioxidants recovery. Then, a Box-Behnken design was applied considering as independent variables the solvent composition (3070% ethanol in water), temperature (3070 °C) and time (3060 min), in order to evaluate the effects of these factors on antioxidant activity in juçara extract. Results showed that the extracts with higher antioxidant activity were obtained using 30% ethanol at 70 °C for 60 min; measurements included ABTS and DPPH assays, determination of total phenolic content and total monomeric anthocyanins. Furthermore, the effect of pH in antioxidants recovery was evaluated. For this purpose, the 30% ethanol solution was acidified to pH 1 and 2 with HCl. Principal component analysis showed the formation of three distinct groups: one characterized by high bioactive compounds content (pH 1.0), another with superior antioxidant activity (pH 5.75, non-acidified), and finally the group at pH 2 presenting the worst concentrations in the evaluated responses. HPLC analysis showed the presence of cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside in the extracts. Therefore, the conventional solid-liquid extraction using renewable solvent can be successfully applied to recover bioactive compounds from juçara residue, which can be used by different food industries.The authors gratefully acknowledge the institutions: Coordenação de Aperfeiçoamento Pessoal deEnsinoSuperior (CAPES), Universidade Federal do Rio de Janeiro, Embrapa Agroindústria de Alimentos and University of Minho by the financial support of the research work and Juçaí Alimentos for the juçara residue. Ricardo N. Pereira gratefully acknowledge to Portuguese Foundation for Science and Technology (FCT) the financial grant with reference SFRH/BPD/ 81887/2011.info:eu-repo/semantics/publishedVersio

Portuguese Football Federation consensus statement 2020: nutrition and performance in football

Nutrition is an undeniable part of promoting health and performance among football (soccer) players. Nevertheless, nutritional strategies adopted in elite football can vary significantly depending on culture, habit and practical constraints and might not always be supported by scientific evidence. Therefore, a group of 28 Portuguese experts on sports nutrition, sports science and sports medicine sought to discuss current practices in the elite football landscape and review the existing evidence on nutritional strategies to be applied when supporting football players. Starting from understanding football's physical and physiological demands, five different moments were identified: preparing to play, match-day, recovery after matches, between matches and during injury or rehabilitation periods. When applicable, specificities of nutritional support to young athletes and female players were also addressed. The result is a set of practical recommendations that gathered consensus among involved experts, highlighting carbohydrates periodisation, hydration and conscious use of dietary supplements.info:eu-repo/semantics/publishedVersio

Activation of Type 1 Cannabinoid Receptor (CB1R) promotes neurogenesis in murine subventricular zone cell cultures

The endocannabinoid system has been implicated in the modulation of adult neurogenesis. Here, we describe the effect of type 1 cannabinoid receptor (CB1R) activation on self-renewal, proliferation and neuronal differentiation in mouse neonatal subventricular zone (SVZ) stem/progenitor cell cultures. Expression of CB1R was detected in SVZ-derived immature cells (Nestin-positive), neurons and astrocytes. Stimulation of the CB1R by (R)-(+)-Methanandamide (R-m-AEA) increased self-renewal of SVZ cells, as assessed by counting the number of secondary neurospheres and the number of Sox2+/+ cell pairs, an effect blocked by Notch pathway inhibition. Moreover, R-m-AEA treatment for 48 h, increased proliferation as assessed by BrdU incorporation assay, an effect mediated by activation of MAPK-ERK and AKT pathways. Surprisingly, stimulation of CB1R by R-m-AEA also promoted neuronal differentiation (without affecting glial differentiation), at 7 days, as shown by counting the number of NeuN-positive neurons in the cultures. Moreover, by monitoring intracellular calcium concentrations ([Ca2+](i)) in single cells following KCl and histamine stimuli, a method that allows the functional evaluation of neuronal differentiation, we observed an increase in neuronal-like cells. This proneurogenic effect was blocked when SVZ cells were co-incubated with R-m-AEA and the CB1R antagonist AM 251, for 7 days, thus indicating that this effect involves CB1R activation. In accordance with an effect on neuronal differentiation and maturation, R-m-AEA also increased neurite growth, as evaluated by quantifying and measuring the number of MAP2-positive processes. Taken together, these results demonstrate that CB1R activation induces proliferation, self-renewal and neuronal differentiation from mouse neonatal SVZ cell cultures.Fundacao para a Ciencia e a Tecnologia - Portugal [POCTI/SAU-NEU/68465/2006, PTDC/SAU-NEU/104415/2008, PTDC/SAU-NEU/101783/2008, POCTI/SAU-NEU/110838/2009]; Fundacao Calouste Gulbenkian [96542]; Fundacao para a Ciencia e Tecnologiainfo:eu-repo/semantics/publishedVersio

A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing

Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. Results: We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at http://www.corkoakdb.org. Conclusions: This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.Peer Reviewe