322 research outputs found
A role for dystroglycan in the pathophysiology of acute leukemic cells.
AIMS: Previous reports have demonstrated that alterations or reduced expression of Dystroglycan (Dg) complex (αDg and βDg subunits) are related to progression and severity of neoplastic solid tissues. Therefore we determined the expression pattern and subcellular distribution of Dg complex in Acute Myeloid Leukemia (AML) primary blasts (M1, M2, and M3 phenotypes), as well as HL-60 and Kasumi-1 leukemia cell lines. Additionally, we evaluated the relative expression of the main enzymes controlling α-Dg glycosylation to ascertain the post-translational modifications in the leukemia cell phenotype. MAIN METHODS: Primary leukemia blasts and leukemia cell lines were processed by confocal analysis to determine the subcellular distribution of α-Dg, β-Dg, and phosphorylated β-Dg (Y892), to evaluate the expression pattern of the different Dg species we performed Western Blot (WB) assays, while the messenger RNA (mRNA) expression of enzymes involved in α-Dg glycosylation, such as POMGnT1, POMT1, POMT2, LARGE, FKTN, and FKRP, were evaluated by qualitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). Finally, in an attempt to ameliorate the leukemia cell phenotype, we transfected leukemia cells with a plasmid expressing the Dg complex. KEY FINDINGS: The Dg complex was altered in leukemia cells, including decreased mRNA, protein, and α-Dg glycosylated levels, mislocalization of β-Dg, and a diminution of mRNA expression of LARGE in patients leukemia blasts and in cell lines. Interestingly, the exogenous expression of Dg complex promoted filopodial formation, differentiation, and diminished proliferation, attenuating some HL-60 and Kasumi cells characteristics. SIGNIFICANCE: Dg complex integrity and balance are required for a proper hematopoietic cell function, in that its disruption might contribute to leukemia pathophysiology
Search for W' bosons decaying to an electron and a neutrino with the D0 detector
This Letter describes the search for a new heavy charged gauge boson W'
decaying into an electron and a neutrino. The data were collected with the D0
detector at the Fermilab Tevatron proton-antiproton Collider at a
center-of-mass energy of 1.96 TeV, and correspond to an integrated luminosity
of about 1 inverse femtobarn. Lacking any significant excess in the data in
comparison with known processes, an upper limit is set on the production cross
section times branching fraction, and a W' boson with mass below 1.00 TeV can
be excluded at the 95% C.L., assuming standard-model-like couplings to
fermions. This result significantly improves upon previous limits, and is the
most stringent to date.Comment: submitted to Phys. Rev. Let
Measurement of the ratios of the Z/G* + >= n jet production cross sections to the total inclusive Z/G* cross section in ppbar collisions at sqrt(s) = 1.96 TeV
We present a study of events with Z bosons and jets produced at the Fermilab
Tevatron Collider in ppbar collisions at a center of mass energy of 1.96 TeV.
The data sample consists of nearly 14,000 Z/G* -> e+e- candidates corresponding
to the integrated luminosity of 0.4 fb-1 collected using the D0 detector.
Ratios of the Z/G* + >= n jet cross sections to the total inclusive Z/G* cross
section have been measured for n = 1 to 4 jet events. Our measurements are
found to be in good agreement with a next-to-leading order QCD calculation and
with a tree-level QCD prediction with parton shower simulation and
hadronization.Comment: 7 pages, 2 figures, slightly modified, submitted to Phys. Lett.
Search for single top quarks in the tau+jets channel using 4.8 fb of collision data
We present the first direct search for single top quark production using tau
leptons. The search is based on 4.8 fb of integrated luminosity
collected in collisions at =1.96 TeV with the D0 detector
at the Fermilab Tevatron Collider. We select events with a final state
including an isolated tau lepton, missing transverse energy, two or three jets,
one or two of them tagged. We use a multivariate technique to discriminate
signal from background. The number of events observed in data in this final
state is consistent with the signal plus background expectation. We set in the
tau+jets channel an upper limit on the single top quark cross section of
\TauLimObs pb at the 95% C.L. This measurement allows a gain of 4% in expected
sensitivity for the observation of single top production when combining it with
electron+jets and muon+jets channels already published by the D0 collaboration
with 2.3 fb of data. We measure a combined cross section of
\SuperCombineXSall pb, which is the most precise measurement to date.Comment: 12 pages, 5 figure
b-Jet Identification in the D0 Experiment
Algorithms distinguishing jets originating from b quarks from other jet
flavors are important tools in the physics program of the D0 experiment at the
Fermilab Tevatron p-pbar collider. This article describes the methods that have
been used to identify b-quark jets, exploiting in particular the long lifetimes
of b-flavored hadrons, and the calibration of the performance of these
algorithms based on collider data.Comment: submitted to Nuclear Instruments and Methods in Physics Research
Search for pair production of the scalar top quark in the electron-muon final state
We report the result of a search for the pair production of the lightest
supersymmetric partner of the top quark () in
collisions at a center-of-mass energy of 1.96 TeV at the Fermilab Tevatron
collider corresponding to an integrated luminosity of 5.4 fb. The scalar
top quarks are assumed to decay into a quark, a charged lepton, and a
scalar neutrino (), and the search is performed in the electron
plus muon final state. No significant excess of events above the standard model
prediction is detected, and improved exclusion limits at the 95% C.L. are set
in the the (,) mass plane
Measurement of the dijet invariant mass cross section in proton anti-proton collisions at sqrt{s} = 1.96 TeV
The inclusive dijet production double differential cross section as a
function of the dijet invariant mass and of the largest absolute rapidity of
the two jets with the largest transverse momentum in an event is measured in
proton anti-proton collisions at sqrt{s} = 1.96 TeV using 0.7 fb^{-1}
integrated luminosity collected with the D0 detector at the Fermilab Tevatron
Collider. The measurement is performed in six rapidity regions up to a maximum
rapidity of 2.4. Next-to-leading order perturbative QCD predictions are found
to be in agreement with the data.Comment: Published in Phys. Lett. B, 693, (2010), 531-538, 8 pages, 2 figures,
6 table
Measurement of Z/gamma*+jet+X angular distributions in ppbar collisions at sqrt{s}=1.96 TeV
We present the first measurements at a hadron collider of differential cross
sections for Z+jet+X production in delta phi(Z, jet), |delta y(Z, jet)| and
|y_boost(Z, jet)|. Vector boson production in association with jets is an
excellent probe of QCD and constitutes the main background to many small cross
section processes, such as associated Higgs production. These measurements are
crucial tests of the predictions of perturbative QCD and current event
generators, which have varied success in describing the data. Using these
measurements as inputs in tuning event generators will increase the
experimental sensitivity to rare signals.Comment: Published in Physics Letters B 682 (2010), pp. 370-380. 15 pages, 6
figure
Search for the standard model Higgs boson in tau final states
We present a search for the standard model Higgs boson using hadronically
decaying tau leptons, in 1 inverse femtobarn of data collected with the D0
detector at the Fermilab Tevatron ppbar collider. We select two final states:
tau plus missing transverse energy and b jets, and tau+ tau- plus jets. These
final states are sensitive to a combination of associated W/Z boson plus Higgs
boson, vector boson fusion and gluon-gluon fusion production processes. The
observed ratio of the combined limit on the Higgs production cross section at
the 95% C.L. to the standard model expectation is 29 for a Higgs boson mass of
115 GeV.Comment: publication versio
Search for scalar bottom quarks and third-generation leptoquarks in ppbar collisions at sqrt(s) = 1.96 TeV
We report the results of a search for pair production of scalar bottom quarks
(sbottom) and scalar third-generation leptoquarks in 5.2 fb-1 of ppbar
collisions at the D0 experiment of the Fermilab Tevatron Collider. Scalar
bottom quarks are assumed to decay to a neutralino and a quark, and we set
95% C.L. lower limits on their production in the (m_sbottom, m_neutralino) mass
plane such as m_sbottom>247 GeV for m_neutralino=0 and m_neutralino>110 GeV for
160<m_sbottom<200 GeV. The leptoquarks are assumed to decay to a tau neutrino
and a quark, and we set a 95% C.L. lower limit of 247 GeV on the mass of a
charge-1/3 third-generation scalar leptoquark.Comment: Published by Phys. Lett.
- …