16 research outputs found

    Sampling, isolating and identifying microplastics ingested by fish and invertebrates

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    Microplastic debris (<5 mm) is a prolific environmental pollutant, found worldwide in marine, freshwater and terrestrial ecosystems. Interactions between biota and microplastics are prevalent, and there is growing evidence that microplastics can incite significant health effects in exposed organisms. To date, the methods used to quantify such interactions have varied greatly between studies. Here, we critically review methods for sampling, isolating and identifying microplastics ingested by environmentally and laboratory exposed fish and invertebrates. We aim to draw attention to the strengths and weaknesses of the suite of published microplastic extraction and enumeration techniques. Firstly, we highlight the risk of microplastic losses and accumulation during biotic sampling and storage, and suggest protocols for mitigating contamination in the field and laboratory. We evaluate a suite of methods for extracting microplastics ingested by biota, including dissection, depuration, digestion and density separation. Lastly, we consider the applicability of visual identification and chemical analyses in categorising microplastics. We discuss the urgent need for the standardisation of protocols to promote consistency in data collection and analysis. Harmonized methods will allow for more accurate assessment of the impacts and risks microplastics pose to biota and increase comparability between studies

    Dark energy survey year 3 results: cosmology with peaks using an emulator approach

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    We constrain the matter density ωm and the amplitude of density fluctuations σ8 within the ΛCDM cosmological model with shear peak statistics and angular convergence power spectra using mass maps constructed from the first three years of data of the Dark Energy Survey (DES Y3). We use tomographic shear peak statistics, including cross-peaks: peak counts calculated on maps created by taking a harmonic space product of the convergence of two tomographic redshift bins. Our analysis follows a forward-modelling scheme to create a likelihood of these statistics using N-body simulations, using a Gaussian process emulator. We take into account the uncertainty from the remaining, largely unconstrained ΛCDM parameters (ωb, ns, and h). We include the following lensing systematics: multiplicative shear bias, photometric redshift uncertainty, and galaxy intrinsic alignment. Stringent scale cuts are applied to avoid biases from unmodelled baryonic physics. We find that the additional non-Gaussian information leads to a tightening of the constraints on the structure growth parameter yielding S8 σ8√Ωm/0.3=0.797-0.013+0.015 (68 per cent confidence limits), with a precision of 1.8 per cent, an improvement of 38 per cent compared to the angular power spectra only case. The results obtained with the angular power spectra and peak counts are found to be in agreement with each other and no significant difference in S8 is recorded. We find a mild tension of 1.5 σ between our study and the results from Planck 2018, with our analysis yielding a lower S8. Furthermore, we observe that the combination of angular power spectra and tomographic peak counts breaks the degeneracy between galaxy intrinsic alignment AIA and S8, improving cosmological constraints. We run a suite of tests concluding that our results are robust and consistent with the results from other studies using DES Y3 data

    Microplastics exposures of fish: internalization and effects on behavior and growth

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    International audienceAwareness of the presence of microplastics, i.e. plastic particles ranging in size from 1 ÎŒm to 5 mm, in marine and freshwaters has recently risen but detection and quantification is challenging. Furthermore, whether they pose a risk to aquatic organisms is not yet clear. Fish, for example, have been demonstrated to ingest microplastics particles but the link between quantification of uptake and impact assessment has not yet been made. In this context, we are exploring methods for particles quantification upon feeding juvenile fish with regular food and microplastics and assess whether exposure impacts behavior and growth. For quantification of uptake, we hypothesized that it is possible to analyze the fish tissue by flow cytometry in combination with viSNE, which allows the 2D clustering of particles with different features according to the fluorescence measured. Exposure experiments were carried out for up to three weeks, using different types of microplastic particles and a wide concentration range. In the flow, when particles were mixed with fish tissue, flow cytometry/viSNE was able to differentiate particles natures, numbers and sizes. About 10% of added particles were internalized by the fish from all particles that floated or settled on the bottom. Particles ingestion resulted in a slight impact on behavior. Yet, floating particles were massively incorporated by the fish and significant numbers remained even after 24h of depuration. Based on this, we are currently exploring if continuous feeding with microparticles contaminated food has consequences on juvenile fish growth. Taken together, our study demonstrates the power and limits of flow cytometry/viSNE for microplastics quantification in a complex biological matrix like fish.The setup could be extended to other types and forms of microplastics in different environmental matrices. Moreover, our study sheds light on ecological consequences that microplastics exposure might have on fish

    Between adoption and rejection: attitudes of adult educators toward digitization in Germany

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    The use of digital media in adult education is very heterogene-ous. To date, there are no empirical studies that have examined the possibility that media-related differences in media usage of adult educators could be in part due to differential media pedagogical attitudes of adult educators. Moreover, there is a lack of empirical evidence to support the understanding of what factors modulate differences in media pedagogical com-petencies of adult educators. In order to examine different the-oretical potentialities, in the present study, an online survey of adult educators (n = 626) was conducted to investigate the attitudes of adult educators in Germany toward their use of digital media. The results of the study indicate that there are influencing factors such as educational level or employment context on attitudes toward digital media
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