415 research outputs found
Cellular localization of the potassium channel Kir7.1 in guinea pig and human kidney
Cellular localization of the potassium channel Kir7.1 in guinea pig and human kidney.BackgroundK+ channels have important functions in the kidney, such as maintenance of the membrane potential, volume regulation, recirculation, and secretion of potassium ions. The aim of this study was to obtain more information on the localization and possible functional role of the inwardly rectifying K+ channel, Kir7.1.MethodsKir7.1 cDNA (1114 bp) was isolated from guinea pig kidney (gpKir7.1), and its tissue distribution was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, a genomic DNA fragment (6153 bp) was isolated from a genomic library. cRNA was expressed in Xenopus laevis oocytes for functional studies. Immunohistochemistry and RT-PCR were used to localize Kir7.1 in guinea pig and human kidney.ResultsThe expression of gpKir7.1 in Xenopus laevis oocytes revealed inwardly rectifying K+ currents. The reversal potential was strongly dependent on the extracellular K+ concentration, shifting from -14 mV at 96 mmol/L K+ to -90 mV at 1 mmol/L K+. gpKir7.1 showed a low affinity for Ba2+. Significant expression of gpKir7.1 was found in brain, kidney, and lung, but not in heart, skeletal muscle, liver, or spleen. Immunocytochemical detection in guinea pig identified the gpKir7.1 protein in the basolateral membrane of epithelial cells of the proximal tubule. RT-PCR analysis identified strong gpKir7.1 expression in the proximal tubule and weak expression in glomeruli and thick ascending limb. In isolated human tubule fragments, RT-PCR showed expression in proximal tubule and thick ascending limb.ConclusionOur results suggest that Kir7.1 may contribute to basolateral K+ recycling in the proximal tubule and in the thick ascending limb
Detailed spectral and morphological analysis of the shell type SNR RCW 86
Aims: We aim for an understanding of the morphological and spectral
properties of the supernova remnant RCW~86 and for insights into the production
mechanism leading to the RCW~86 very high-energy gamma-ray emission. Methods:
We analyzed High Energy Spectroscopic System data that had increased
sensitivity compared to the observations presented in the RCW~86 H.E.S.S.
discovery publication. Studies of the morphological correlation between the
0.5-1~keV X-ray band, the 2-5~keV X-ray band, radio, and gamma-ray emissions
have been performed as well as broadband modeling of the spectral energy
distribution with two different emission models. Results:We present the first
conclusive evidence that the TeV gamma-ray emission region is shell-like based
on our morphological studies. The comparison with 2-5~keV X-ray data reveals a
correlation with the 0.4-50~TeV gamma-ray emission.The spectrum of RCW~86 is
best described by a power law with an exponential cutoff at TeV and a spectral index of ~. A static
leptonic one-zone model adequately describes the measured spectral energy
distribution of RCW~86, with the resultant total kinetic energy of the
electrons above 1 GeV being equivalent to 0.1\% of the initial kinetic
energy of a Type I a supernova explosion. When using a hadronic model, a
magnetic field of ~100G is needed to represent the measured data.
Although this is comparable to formerly published estimates, a standard
E spectrum for the proton distribution cannot describe the gamma-ray
data. Instead, a spectral index of ~1.7 would be required, which
implies that ~erg has been transferred into
high-energy protons with the effective density cm^-3. This
is about 10\% of the kinetic energy of a typical Type Ia supernova under the
assumption of a density of 1~cm^-3.Comment: accepted for publication by A&
Characterizing the gamma-ray long-term variability of PKS 2155-304 with H.E.S.S. and Fermi-LAT
Studying the temporal variability of BL Lac objects at the highest energies
provides unique insights into the extreme physical processes occurring in
relativistic jets and in the vicinity of super-massive black holes. To this
end, the long-term variability of the BL Lac object PKS 2155-304 is analyzed in
the high (HE, 100 MeV 200 GeV)
gamma-ray domain. Over the course of ~9 yr of H.E.S.S observations the VHE
light curve in the quiescent state is consistent with a log-normal behavior.
The VHE variability in this state is well described by flicker noise
(power-spectral-density index {\ss}_VHE = 1.10 +0.10 -0.13) on time scales
larger than one day. An analysis of 5.5 yr of HE Fermi LAT data gives
consistent results ({\ss}_HE = 1.20 +0.21 -0.23, on time scales larger than 10
days) compatible with the VHE findings. The HE and VHE power spectral densities
show a scale invariance across the probed time ranges. A direct linear
correlation between the VHE and HE fluxes could neither be excluded nor firmly
established. These long-term-variability properties are discussed and compared
to the red noise behavior ({\ss} ~ 2) seen on shorter time scales during
VHE-flaring states. The difference in power spectral noise behavior at VHE
energies during quiescent and flaring states provides evidence that these
states are influenced by different physical processes, while the compatibility
of the HE and VHE long-term results is suggestive of a common physical link as
it might be introduced by an underlying jet-disk connection.Comment: 11 pages, 16 figure
Detection of variable VHE gamma-ray emission from the extra-galactic gamma-ray binary LMC P3
Context. Recently, the high-energy (HE, 0.1-100 GeV) -ray emission
from the object LMC P3 in the Large Magellanic Cloud (LMC) has been discovered
to be modulated with a 10.3-day period, making it the first extra-galactic
-ray binary.
Aims. This work aims at the detection of very-high-energy (VHE, >100 GeV)
-ray emission and the search for modulation of the VHE signal with the
orbital period of the binary system.
Methods. LMC P3 has been observed with the High Energy Stereoscopic System
(H.E.S.S.); the acceptance-corrected exposure time is 100 h. The data set has
been folded with the known orbital period of the system in order to test for
variability of the emission. Energy spectra are obtained for the orbit-averaged
data set, and for the orbital phase bin around the VHE maximum.
Results. VHE -ray emission is detected with a statistical
significance of 6.4 . The data clearly show variability which is
phase-locked to the orbital period of the system. Periodicity cannot be deduced
from the H.E.S.S. data set alone. The orbit-averaged luminosity in the
TeV energy range is erg/s. A luminosity of erg/s is reached during 20% of the orbit. HE and VHE
-ray emissions are anti-correlated. LMC P3 is the most luminous
-ray binary known so far.Comment: 5 pages, 3 figures, 1 table, accepted for publication in A&
The exceptionally powerful TeV gamma-ray emitters in the Large Magellanic Cloud
The Large Magellanic Cloud, a satellite galaxy of the Milky Way, has been
observed with the High Energy Stereoscopic System (H.E.S.S.) above an energy of
100 billion electron volts for a deep exposure of 210 hours. Three sources of
different types were detected: the pulsar wind nebula of the most energetic
pulsar known N 157B, the radio-loud supernova remnant N 132D and the largest
non-thermal X-ray shell - the superbubble 30 Dor C. The unique object SN 1987A
is, surprisingly, not detected, which constrains the theoretical framework of
particle acceleration in very young supernova remnants. These detections reveal
the most energetic tip of a gamma-ray source population in an external galaxy,
and provide via 30 Dor C the unambiguous detection of gamma-ray emission from a
superbubble.Comment: Published in Science Magazine (Jan. 23, 2015). This ArXiv version has
the supplementary online material incorporated as an appendix to the main
pape
Combining least absolute shrinkage and selection operator (LASSO) and principal-components analysis for detection of gene-gene interactions in genome-wide association studies
Constraints on the intergalactic magnetic field using Fermi-LAT and H.E.S.S. blazar observations
Magnetic fields in galaxies and galaxy clusters are believed to be the result
of the amplification of intergalactic seed fields during the formation of
large-scale structures in the universe. However, the origin, strength, and
morphology of this intergalactic magnetic field (IGMF) remain unknown. Lower
limits on (or indirect detection of) the IGMF can be obtained from observations
of high-energy gamma rays from distant blazars. Gamma rays interact with the
extragalactic background light to produce electron-positron pairs, which can
subsequently initiate electromagnetic cascades. The -ray signature of
the cascade depends on the IGMF since it deflects the pairs. Here we report on
a new search for this cascade emission using a combined data set from the Fermi
Large Area Telescope and the High Energy Stereoscopic System. Using
state-of-the-art Monte Carlo predictions for the cascade signal, our results
place a lower limit on the IGMF of G for a coherence
length of 1 Mpc even when blazar duty cycles as short as 10 yr are assumed.
This improves on previous lower limits by a factor of 2. For longer duty cycles
of () yr, IGMF strengths below G
( G) are excluded, which rules out specific models for IGMF
generation in the early universe.Comment: 20 pages, 7 figures, 4 tables. Accepted for publication in ApJ
Letters. Auxiliary data is provided in electronic format at
https://zenodo.org/record/801431
A Genome-Wide Homozygosity Association Study Identifies Runs of Homozygosity Associated with Rheumatoid Arthritis in the Human Major Histocompatibility Complex
Rheumatoid arthritis (RA) is a chronic inflammatory disorder with a polygenic mode of inheritance. This study examined the hypothesis that runs of homozygosity (ROHs) play a recessive-acting role in the underlying RA genetic mechanism and identified RA-associated ROHs. Ours is the first genome-wide homozygosity association study for RA and characterized the ROH patterns associated with RA in the genomes of 2,000 RA patients and 3,000 normal controls of the Wellcome Trust Case Control Consortium. Genome scans consistently pinpointed two regions within the human major histocompatibility complex region containing RA-associated ROHs. The first region is from 32,451,664 bp to 32,846,093 bp (−log10(p)>22.6591). RA-susceptibility genes, such as HLA-DRB1, are contained in this region. The second region ranges from 32,933,485 bp to 33,585,118 bp (−log10(p)>8.3644) and contains other HLA-DPA1 and HLA-DPB1 genes. These two regions are physically close but are located in different blocks of linkage disequilibrium, and ∼40% of the RA patients' genomes carry these ROHs in the two regions. By analyzing homozygote intensities, an ROH that is anchored by the single nucleotide polymorphism rs2027852 and flanked by HLA-DRB6 and HLA-DRB1 was found associated with increased risk for RA. The presence of this risky ROH provides a 62% accuracy to predict RA disease status. An independent genomic dataset from 868 RA patients and 1,194 control subjects of the North American Rheumatoid Arthritis Consortium successfully validated the results obtained using the Wellcome Trust Case Control Consortium data. In conclusion, this genome-wide homozygosity association study provides an alternative to allelic association mapping for the identification of recessive variants responsible for RA. The identified RA-associated ROHs uncover recessive components and missing heritability associated with RA and other autoimmune diseases
A deep spectromorphological study of the -ray emission surrounding the young massive stellar cluster Westerlund 1
Young massive stellar clusters are extreme environments and potentially
provide the means for efficient particle acceleration. Indeed, they are
increasingly considered as being responsible for a significant fraction of
cosmic rays (CRs) accelerated within the Milky Way. Westerlund 1, the most
massive known young stellar cluster in our Galaxy is a prime candidate for
studying this hypothesis. While the very-high-energy -ray source HESS
J1646-458 has been detected in the vicinity of Westerlund 1 in the past, its
association could not be firmly identified. We aim to identify the physical
processes responsible for the -ray emission around Westerlund 1 and
thus to better understand the role of massive stellar clusters in the
acceleration of Galactic CRs. Using 164 hours of data recorded with the High
Energy Stereoscopic System (H.E.S.S.), we carried out a deep
spectromorphological study of the -ray emission of HESS J1646-458. We
furthermore employed H I and CO observations of the region to infer the
presence of gas that could serve as target material for interactions of
accelerated CRs. We detected large-scale ( diameter) -ray
emission with a complex morphology, exhibiting a shell-like structure and
showing no significant variation with -ray energy. The combined energy
spectrum of the emission extends to several tens of TeV, and is uniform across
the entire source region. We did not find a clear correlation of the
-ray emission with gas clouds as identified through H I and CO
observations. We conclude that, of the known objects within the region, only
Westerlund 1 can explain the bulk of the -ray emission. Several CR
acceleration sites and mechanisms are conceivable, and discussed in detail.
(abridged)Comment: 15 pages, 9 figures. Corresponding authors: L. Mohrmann, S. Ohm, R.
Rauth, A. Specoviu
HESS J1809193: a halo of escaped electrons around a pulsar wind nebula?
Context. HESS J1809193 is an unassociated very-high-energy -ray
source located on the Galactic plane. While it has been connected to the nebula
of the energetic pulsar PSR J18091917, supernova remnants and molecular
clouds present in the vicinity also constitute possible associations. Recently,
the detection of -ray emission up to energies of 100 TeV with the
HAWC observatory has led to renewed interest in HESS J1809193.
Aims. We aim to understand the origin of the -ray emission of HESS
J1809193.
Methods. We analysed 93.2 h of data taken on HESS J1809193 above 0.27 TeV
with the High Energy Stereoscopic System (H.E.S.S.), using a multi-component,
three-dimensional likelihood analysis. In addition, we provide a new analysis
of 12.5 yr of Fermi-LAT data above 1 GeV within the region of HESS J1809193.
The obtained results are interpreted in a time-dependent modelling framework.
Results. For the first time, we were able to resolve the emission detected
with H.E.S.S. into two components: an extended component that exhibits a
spectral cut-off at 13 TeV, and a compact component that is located close
to PSR J18091917 and shows no clear spectral cut-off. The Fermi-LAT analysis
also revealed extended -ray emission, on scales similar to that of the
extended H.E.S.S. component.
Conclusions. Our modelling indicates that based on its spectrum and spatial
extent, the extended H.E.S.S. component is likely caused by inverse Compton
emission from old electrons that form a halo around the pulsar wind nebula. The
compact component could be connected to either the pulsar wind nebula or the
supernova remnant and molecular clouds. Due to its comparatively steep
spectrum, modelling the Fermi-LAT emission together with the H.E.S.S.
components is not straightforward. (abridged)Comment: 14 pages, 10 figures. Accepted for publication in A&A. Corresponding
authors: Vikas Joshi, Lars Mohrman
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