EAIMS: Emergency Analysis Identification and Management System

Social media has great potential as a means to enable civil protection and law enforcement agencies to more effectively tackle disasters and emergencies. However, there is currently a lack of tools that enable civil protection agencies to easily make use of social media. The Emergency Analysis Identification and Management System (EAIMS) is a prototype service that provides real-time detection of emergency events, related information finding and credibility analysis tools for use over social media during emergencies. This system exploits machine learning over data gathered from past emergencies and disasters to build effective models for identifying new events as they occur, tracking developments within those events and analyzing those developments for the purposes of enhancing the decision making processes of emergency response agencies

The IMERAPlus Joint Research Project For Determinations Of The Boltzmann Constant

Abstract. To provide new determinations of the Boltzmann constant, k, which has been asked for by the International Committee for Weights and Measures concerning preparative steps towards new definitions of the kilogram, the ampere, the kelvin and the mole, an iMERAPlus joint research project has coordinated the European activities in this field. In this major European research project the Boltzmann constant has been determined by various methods to support the new definition of the kelvin. The final results of the project are reviewed in this paper. Determinations of the Boltzmann constant k were achieved within the project by all three envisaged methods: acoustic gas thermometry, Doppler broadening technique, and dielectric constant gas thermometry. The results were exploited by the interdisciplinary Committee on Data for Science and Technology (CODATA) in their 2010 adjustment of recommended values for fundamental constants. As a result, the CODATA group recommended a value for k with a relative standard uncertainty about a factor of two smaller than the previous u(k)/k of 1.7×10 −6

CMS physics technical design report : Addendum on high density QCD with heavy ions

Peer reviewe

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Gene transfer to rat cerebral cortex mediated by polysorbate 80 and poloxamer 188 nonionic surfactant vesicles

Background: Gene therapy can be an intriguing therapeutic option in wide-ranging neurological disorders. Though nonviral gene carriers represent a safer delivery system to their viral counterparts, a thorough design of such vehicles is crucial to enhance their transfection properties. Purpose: This study evaluated the effects of combined use of two nonionic surfactants, poloxamer 188 (P) and polysorbate 80 (P80) into nanovesicles – based on 2,3-di(tetradecyloxy)propan-1-amine cationic lipid (D) – destined for gene delivery to central nervous system cells. Methods: Niosome formulations without and with poloxamer 188 (DP80 and DPP80, respectively) were prepared by the reverse-phase evaporation technique and characterized in terms of size, surface charge, and morphology. After the addition of pCMS-EGFP plasmid, the binding efficiency to the niosomes was evaluated in agarose gel electrophoresis assays. Additionally, transfection efficiency of complexes was also evaluated in in vitro and in vivo conditions. Results: In vitro experiments on NT2 cells revealed that the complexes based on a surfactant combination (DPP80) enhanced cellular uptake and viability when compared with the DP80 counterparts. Interestingly, DPP80 complexes showed protein expression in glial cells after administration into the cerebral cortices of rats. Conclusion: These data provide new insights for glia-centered approach for gene therapy of nervous system disorders using cationic nanovesicles, where nonionic surfactants play a pivotal role.This project was supported by the Basque Country Government (CGIC10/172), Spanish Ministry of Education (Grant CTQ2017-84415-R, MAT2015-69967-C3-1R), the Generalitat de Catalunya (2014/SGR/624), and the Instituto de Salud Carlos III (CB06_01_0019, CB06_01_1028). The authors also wish to thank the intellectual and technical assistance from the ICTS “NANBIOSIS”, more specifically by the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN) at the University of Basque Country (UPV/EHU). Technical and human support provided by SGIker (UPV/EHU) is gratefully acknowledged. Jose Luis Pedraz and Gustavo Puras are corresponding authors for this study.Peer reviewe

Non-viral vectors based on cationic niosomes and minicircle DNA technology enhance gene delivery efficiency for biomedical applications in retinal disorders

11 pags., 6 figs.Low transfection efficiency is a major challenge to overcome in non-viral approaches to reach clinical practice. Our aim was to explore new strategies to achieve more efficient non-viral gene therapies for clinical applications and in particular, for retinal diseases. Cationic niosomes and three GFP-encoding genetic materials consisting on minicircle (2.3 kb), its parental plasmid (3.5 kb) and a larger plasmid (5.5 kb) were combined to form nioplexes. Once fully physicochemically characterized, in vitro experiments in ARPE-19 retina epithelial cells showed that transfection efficiency of minicircle nioplexes doubled that of plasmids ones, maintaining good cell viability in all cases. Transfections in retinal primary cells and injections of nioplexes in rat retinas confirmed the higher capacity of cationic niosomes vectoring minicircle to deliver the genetic material into retina cells. Therefore, nioplexes based on cationic niosomes vectoring minicircle DNA represent a potential tool for the treatment of inherited retinal diseases.This work was supported by the Basque Country Government (Department of Education, University and Research, pre-doctoral grant PRE_2016_2_0302 and Consolidated Groups, IT907-16), the Ikerbasque Foundation for Science from the Basque Country, the Spanish Grant MAT 2015-69976-C3-1, SAF2013-42347-R, and by the Research Chair“Bidons Egara