Polyester-polyvinylalcohol hydrophilization strategies

Poly (ethylene terephthalate) (PET), also commonly called as polyester, is the most widely used polymer for the production of synthetic fibres over the past fifty years. The frequent use of this PET is due to its high mechanical strength combined with other properties such as the resistance to chemical products, stretching and abrasion. The fibre’s hydrophobicity also impacts the difficulty of cleaning these materials [1, 2]. Previous works shows that treatment with concentrated NaOH solutions can greatly improve hydrophilicity of PET fibre [1, 2, 3, 4]. However a significant decrease of mechanical properties takes place during this process. In this work, chemical strategies to counteract this negative effect and further increase the hydrophilicity of PET fibre’s has been tested. In particular, the effect of polyvinyl alcohol (PVA) and N, N ́-dimethylol-4, 5-dihydroxyethyleneurea (DMDHEU) chemically modified resin in the functionalization of saponified PET was carefully analysed. The treated fabrics were characterized by scanning electron microscopy (SEM), contact angle, ATR-FTIR spectroscopy and differential scanning calorimetry (DSC). When the best process conditions were considered for PVA-DMDHEU application, the modified PET presented a contact angle of 33.9o, stain release grade of 4 and a 45.6% increase in its mechanical properties when compared to saponified PET

A New Mutation Causing Progressive Familiar Intrahepatic Cholestasis Type 3 in Association with Autoimmune Hepatitis

Background: Some patients exhibit features of both autoimmune hepatitis (AIH) and primary sclerosing cholangitis (PSC). Similarly, patients with progressive familial intrahepatic cholestasis type 3 (PFIC3) may share histological features with PSC. Case report: We report the case of a 22-year-old man who, since he was 5 years of age, has presented with pruritus, an approximately ninefold elevation of aminotransferases, and γ-glutamyl transferase levels ~10 times the upper limit. Initially he was diagnosed with an overlap syndrome of small duct PSC plus AIH. However, fluctuations in liver enzymes were observed over the following years. Analysis of the ABCB4 gene indicated the diagnosis of PFIC3, revealing a mutation not previously reported. Conclusion: With this case report we aim to describe a new mutation, raise awareness of this rare pathology and highlight the importance of genetic testing of the ABCB4 gene in patients with autoimmune liver disease (mainly small duct PSC) with incomplete response to immunosuppressive treatmen

Instalação de um ensaio de produção intensiva de biomassa lenhosa para energia

No âmbito de um projecto de investigação tendo como propósito o desenvolvimento de sistemas sustentáveis de produção de biomassa lenhosa para energia, foi instalado em Bragança, em 2008, um ensaio de produção intensiva de biomassa lenhosa com base em culturas de curta rotação. Este ensaio tem como objectivos específicos a avaliação dos efeitos de factores como material vegetal, densidade de plantação, rega e fertilização no crescimento e produção de biomassa lenhosa. Nesta comunicação descrevemos a instalação do ensaio relativamente ao desenho experimental seguido, estrutura interna das parcelas de estudo, material vegetal utilizado, operações de preparação do solo e de plantação e ainda gestão da cultura com respeito a fertilização, rega e protecção. Avaliamos também a sobrevivência das plantas no final da primeira estação de crescimento, bem como apresentamos os custos gerais associados à instalação. A apresentação deste trabalho permite partilhar com a comunidade científica e técnica nacional a experiência da instalação deste tipo de culturas, muito recentes no nosso país, e dessa forma contribuir para a definição de processos ajustados e eficientes de instalação e gestão de culturas lenhosas para produção de biomassa para energia em Portugal

Laboratory of technologies and learning of programming and robotics for pre and primary school

This research intends to study how to integrate programming and robotics in kindergartens and primary schools, through a transversal intervention in all the areas of knowledge. This will allow the development of a theoretical framework based on research, able to support the formulation of a proposal of intervention extended to the Portuguese national context. For this project, we will be designing a physical and a mobile laboratory, equipped with tablets and robots, which will facilitate, on the one hand, the initial, postgraduate and continuous training of teachers and, on the other hand, the accomplishment of several case studies throughout Portugal. In addition, the training of teachers and also the participation of the children in the expected activities of this project will enable the designing a theoretical framework about the development of a competences profile for education professionals in this area.This work is funded by CIEd – Research Centre on Education, projects UID/CED/1661/2013 and UID/CED/1661/2016, Institute of Education, University of Minho, through national funds of FCT/MCTES-PT. Fundação para a Ciência e Tecnologia (FCT) – with the reference: SFRH/BPD/109205/2015) – Postdoctoral fellowshipinfo:eu-repo/semantics/publishedVersio

Complete proteome of a quinolone-resistant Salmonella Typhimurium phage type DT104B clinical strain

Salmonellosis is one of the most common and widely distributed foodborne diseases. The emergence of Salmonella strains that are resistant to a variety of antimicrobials is a serious global public health concern. Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) is one of these emerging epidemic multidrug resistant strains. Here we collate information from the diverse and comprehensive range of experiments on Salmonella proteomes that have been published. We then present a new study of the proteome of the quinolone-resistant Se20 strain (phage type DT104B), recovered after ciprofloxacin treatment and compared it to the proteome of reference strain SL1344. A total of 186 and 219 protein spots were recovered from Se20 and SL1344 protein extracts, respectively, after two-dimensional gel electrophoresis. The signatures of 94% of the protein spots were successfully identified through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS). Three antimicrobial resistance related proteins, whose genes were previously detected by polymerase chain reaction (PCR), were identified in the clinical strain. The presence of these proteins, dihydropteroate synthase type-2 (sul2 gene), aminoglycoside resistance protein A (strA gene) and aminoglycoside 6'-N-acetyltransferase type Ib-cr4 (aac(6')-Ib-cr4 gene), was confirmed in the DT104B clinical strain. The aac(6')-Ib-cr4 gene is responsible for plasmid-mediated aminoglycoside and quinolone resistance. This is a preliminary analysis of the proteome of these two S. Typhimurium strains and further work is being developed to better understand how antimicrobial resistance is developing in this pathogen

A small TAT-TrkB peptide prevents BDNF receptor cleavage and restores synaptic physiology in Alzheimer\u27s disease

Copyright \ua9 2024 The Author(s). Published by Elsevier Inc. All rights reserved. In Alzheimer\u27s disease (AD), amyloid β (Aβ)-triggered cleavage of TrkB-FL impairs brain-derived neurotrophic factor (BDNF) signaling, thereby compromising neuronal survival, differentiation, and synaptic transmission and plasticity. Using cerebrospinal fluid and postmortem human brain samples, we show that TrkB-FL cleavage occurs from the early stages of the disease and increases as a function of pathology severity. To explore the therapeutic potential of this disease mechanism, we designed small TAT-fused peptides and screened their ability to prevent TrkB-FL receptor cleavage. Among these, a TAT-TrkB peptide with a lysine-lysine linker prevented TrkB-FL cleavage both in vitro and in vivo and rescued synaptic deficits induced by oligomeric Aβ in hippocampal slices. Furthermore, this TAT-TrkB peptide improved the cognitive performance, ameliorated synaptic plasticity deficits and prevented Tau pathology progression in vivo in the 5XFAD mouse model of AD. No evidence of liver or kidney toxicity was found. We provide proof-of-concept evidence for the efficacy and safety of this therapeutic strategy and anticipate that this TAT-TrkB peptide has the potential to be a disease-modifying drug that can prevent and/or reverse cognitive deficits in patients with AD

Multiomics Assessment of Gene Expression in a Clinical Strain of CTX-M-15-Producing ST131 Escherichia coli

Extended-spectrum beta-lactamase (ESBL)-producing strain C999 was isolated of a Spanish patient with urinary tract infection. Previous genotyping indicated that this strain presented a multidrug-resistance phenotype and carried beta-lactamase genes encoding CTX-M-15, TEM-1, and OXA-1 enzymes. The whole-cell proteome, and the membrane, cytoplasmic, periplasmic and extracellular sub-proteomes of C999 were obtained in this work by two-dimensional gel electrophoresis (2DE) followed by fingerprint sequencing through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). A total of 602 proteins were identified in the different cell fractions, several of which are related to stress response systems, cellular responses, and antibiotic and drug responses, consistent with the multidrug-resistance phenotype. In parallel, whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) was done to identify and quantify the genes present and expressing. The prediction following WGS confirmed our strain as being serotype O25:H4 and sequence type ST131. The presence of proteins related to antibiotic resistance and virulence in an O25:H4-ST131 clone are serious indicators of the continued threat of antibiotic resistance spread amongst healthcare institutions. On a positive note, a multiomics approach can facilitate surveillance and more detailed characterization of virulent bacterial clones from hospital environments

Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis

54/2017). Publisher Copyright: © 2022 by the authors.The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0′) or 15 min (T15′) after incubation, indicating its potential use as a point-of-care method as a screening strategy.publishersversionpublishe

Portuguese recommendations for the use of biological and targeted synthetic diseasemodifying antirheumatic drugs in patients with rheumatoid arthritis – 2020 update

Objective: To update the recommendations for the treatment of rheumatoid arthritis (RA) with biological and targeted synthetic disease-modifying antirheumatic drugs (bDMARDs and tsDMARDs), endorsed by the Portuguese Society of Rheumatology (SPR). Methods: These treatment recommendations were formulated by Portuguese rheumatologists taking into account previous recommendations, new literature evidence and consensus opinion. At a national meeting, in a virtual format, three of the ten previous recommendations were re-addressed and discussed after a more focused literature review. A first draft of the updated recommendations was elaborated by a team of SPR rheumatologists from the SPR rheumatoid arthritis study group, GEAR. The resulting document circulated among all SPR rheumatologists for discussion and input. The level of agreement with each of all the recommendations was anonymously voted online by all SPR rheumatologists. Results: These recommendations cover general aspects such as shared decision, treatment objectives, systematic assessment of disease activity and burden and its registry in Reuma.pt. Consensus was also achieved regarding specific aspects such as initiation of bDMARDs and tsDMARDs, assessment of treatment response, switching and definition of persistent remission. Conclusion: These recommendations may be used for guidance of treatment with bDMARDs and tsDMARDs in patients with RA. As more evidence becomes available and more therapies are licensed, these recommendations will be updated.info:eu-repo/semantics/publishedVersio