Bioactive peptides generated from meat industry by products

There is a large generation of meat by-products, not only from slaughtering but also in the meat industry from trimming and deboning during further processing. This results in extraordinary volumes of by-products that are primarily used as feeds with low returns or, more recently, to biodiesel generation. The aim of this work was to review the state of the art to generate bioactive peptides from meat industry by-products giving them an added value. Hydrolysis with commercial proteases constitutes the typical process and a variety of peptides result from such extensive proteolysis. This review focuses on the potential of meat by-products for the generation of bioactive peptides through enzymatic hydrolysis. The potential of some of the identified peptides to be used as bioactive supplements in foods has also been considered. (C) 2014 Elsevier Ltd. All rights reserved.Grant AGL2010-16305 from MINECO and FEDER funds are fully acknowledged. JAEDOC-CSIC postdoctoral contract to L.M. is also acknowledged. This work was developed under the Unidad Asociada IAD-UPV/IATA-CSIC framework.Mora, L.; Reig Riera, MM.; Toldra, F. (2014). Bioactive peptides generated from meat industry by products. Food Research International. 65:344-349. https://doi.org/10.1016/j.foodres.2014.09.014S3443496

Challenges in the quantitation of the naturally generated bioactive peptides in processed meats

[EN] Background: The final characteristics of processed meats depend on many factors but one of the most important is the intense proteolysis occurred in muscle proteins due to the action of endogenous enzymes in dry-cured ham, and also microbial peptidases in the case of dry-fermented meats, that not only affects taste and flavour but also the generation of bioactive peptides. Scope and approach: In this review main difficulties in the identification of bioactive peptides in processed meats have been described. This study highlights the novel strategies used during the last years to identify naturally generated peptides, and emphasises the need of robust quantitative methodologies for the adequate characterisation of their bioavailability. In fact, the most common and well established quantitation approaches using proteomics are not adapted for peptidomics analysis, so alternative strategies need to be considered. Key findings and conclusions: The progress in the identification and characterisation of the activity of natural bioactive peptides is highly dependent on modern instruments and bioinformatics tools as well as updated protein databases. In fact, the use of in silico approaches and proteomics can be complementary tools in the identification of peptides from meat protein sources as the empirical experimental design can be simplified by using bioinformatics for computer simulation in most of the steps. Finally, Multiple Reaction Monitoring mass spectrometry methodology previously used in the quantitation of therapeutic peptides and biomarkers arises as a powerful tool for absolute quantitation or semiquantitation of bioactive peptides.The research leading to these results received funding from the European Union 7th Framework Programme (FP7/2007-2013) under Grant Agreement 312090 (BACCHUS). This publication reflects only the author views and the Community is not liable for any use made of the information contained therein. Grant AGL2014-57367-R from MINECO and FEDER funds and the Juan de la Cierva postdoctoral contract to LM are acknowledged. The proteomic analysis was performed in the proteomics facility of SCSIE University of Valencia that belongs to ProteoRed, PRB2-ISCIII, (IPT13/0001 - ISCIII-SGEFI/FEDER).Mora Soler, L.; Gallego-Ibáñez, M.; Reig Riera, MM.; Toldrá Vilardell, F. (2017). Challenges in the quantitation of the naturally generated bioactive peptides in processed meats. Trends in Food Science & Technology. 69:306-314. https://doi.org/10.1016/j.tifs.2017.04.011S3063146

Bioaktiiviset peptidit juustoissa ja hapanmaitovalmisteissa

vokET

Bioactive peptides

v2002okET

Bioaktiiviset peptidit

vEK

Bioactive peptides derived from whey proteins: opioid and ACE-inhibitory peptides

vokEK

The isolation and characterization of bioactive peptides derived from bovine milk proteins

vokET

Bioactive peptides derived from bovine whey proteins: opioid and ace-inhibitory peptides

vokEK

Isolation and characterisation of milk-derived bioactive peptides

vokEKTDiss. : Turun yliopisto, 199

Isolation and characterisation of milk-derived bioactive peptides

vokEK