Biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific GTPase Rab11B

Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites

Differential contribution of the m7G-cap to the 5′ end-dependent translation initiation of mammalian mRNAs

Many mammalian mRNAs possess long 5′ UTRs with numerous stem-loop structures. For some of them, the presence of Internal Ribosome Entry Sites (IRESes) was suggested to explain their significant activity, especially when cap-dependent translation is compromised. To test this hypothesis, we have compared the translation initiation efficiencies of some cellular 5′ UTRs reported to have IRES-activity with those lacking IRES-elements in RNA-transfected cells and cell-free systems. Unlike viral IRESes, the tested 5′ UTRs with so-called ‘cellular IRESes’ demonstrate only background activities when placed in the intercistronic position of dicistronic RNAs. In contrast, they are very active in the monocistronic context and the cap is indispensable for their activities. Surprisingly, in cultured cells or cytoplasmic extracts both the level of stimulation with the cap and the overall translation activity do not correlate with the cumulative energy of the secondary structure of the tested 5′ UTRs. The cap positive effect is still observed under profound inhibition of translation with eIF4E-BP1 but its magnitude varies for individual 5′ UTRs irrespective of the cumulative energy of their secondary structures. Thus, it is not mandatory to invoke the IRES hypothesis, at least for some mRNAs, to explain their preferential translation when eIF4E is partially inactivated

Analyse écorégionale marine de Nouvelle-Calédonie : atelier d'identification des aires de conservation prioritaires

Dans le cadre de l'initiative pour les récifs coralliens du Pacifique sud (CRISP), le WWF-France a souhaité développer un projet pour la protection des récifs et des lagons néo-calédoniens. L'atelier, qui s'est déroulé les 10 et 11 août à Nouméa, avait pour objectif de rassembler les scientifiques et les experts du lagon néocalédonien pour identifier, sur la base de leur connaissance experte, les zones les plus remarquables du lagon (richesse, endémisme, originalité des faunes et flores, espèces emblématiques, zones d'intérêt fonctionnel) sur lesquelles doivent porter en priorité les efforts de conservation. Il a permis d'identifier 20 aires prioritaires pour la conservation, parmi lesquelles 6 ont un intérêt mondial, 4 ont un intérêt sur le plan régional, les autres ayant un intérêt local

Happiness around the world: A combined etic-emic approach across 63 countries

What does it mean to be happy? The vast majority of cross-cultural studies on happiness have employed a Western-origin, or "WEIRD" measure of happiness that conceptualizes it as a self-centered (or "independent"), high-arousal emotion. However, research from Eastern cultures, particularly Japan, conceptualizes happiness as including an interpersonal aspect emphasizing harmony and connectedness to others. Following a combined emicetic approach (Cheung, van de Vijver & Leong, 2011), we assessed the cross-cultural applicability of a measure of independent happiness developed in the US (Subjective Happiness Scale; Lyubomirsky & Lepper, 1999) and a measure of interdependent happiness developed in Japan (Interdependent Happiness Scale; Hitokoto & Uchida, 2015), with data from 63 countries representing 7 sociocultural regions. Results indicate that the schema of independent happiness was more coherent in more WEIRD countries. In contrast, the coherence of interdependent happiness was unrelated to a country's "WEIRD-ness." Reliabilities of both happiness measures were lowest in African and Middle Eastern countries, suggesting these two conceptualizations of happiness may not be globally comprehensive. Overall, while the two measures had many similar correlates and properties, the self-focused concept of independent happiness is "WEIRD-er" than interdependent happiness, suggesting cross-cultural researchers should attend to both conceptualizations

Happiness around the world: A combined etic-emic approach across 63 countries.

What does it mean to be happy? The vast majority of cross-cultural studies on happiness have employed a Western-origin, or "WEIRD" measure of happiness that conceptualizes it as a self-centered (or "independent"), high-arousal emotion. However, research from Eastern cultures, particularly Japan, conceptualizes happiness as including an interpersonal aspect emphasizing harmony and connectedness to others. Following a combined emic-etic approach (Cheung, van de Vijver & Leong, 2011), we assessed the cross-cultural applicability of a measure of independent happiness developed in the US (Subjective Happiness Scale; Lyubomirsky & Lepper, 1999) and a measure of interdependent happiness developed in Japan (Interdependent Happiness Scale; Hitokoto & Uchida, 2015), with data from 63 countries representing 7 sociocultural regions. Results indicate that the schema of independent happiness was more coherent in more WEIRD countries. In contrast, the coherence of interdependent happiness was unrelated to a country's "WEIRD-ness." Reliabilities of both happiness measures were lowest in African and Middle Eastern countries, suggesting these two conceptualizations of happiness may not be globally comprehensive. Overall, while the two measures had many similar correlates and properties, the self-focused concept of independent happiness is "WEIRD-er" than interdependent happiness, suggesting cross-cultural researchers should attend to both conceptualizations

The economic well-being of nations is associated with positive daily situational experiences

People in economically advantaged nations tend to evaluate their life as more positive overall and report greater well-being than people in less advantaged nations. But how does positivity manifest in the daily life experiences of individuals around the world? The present study asked 15,244 college students from 62 nations, in 42 languages, to describe a situation they experienced the previous day using the Riverside Situational Q-sort (RSQ). Using expert ratings, the overall positivity of each situation was calculated for both nations and individuals. The positivity of the average situation in each nation was strongly related to the economic development of the nation as measured by the Human Development Index (HDI). For individuals’ daily experiences, the economic status of their nation also predicted the positivity of their experience, even more than their family socioeconomic status. Further analyses revealed the specific characteristics of the average situations for higher HDI nations that make their experiences more positive. Higher HDI was associated with situational experiences involving humor, socializing with others, and the potential to express emotions and fantasies. Lower HDI was associated with an increase in the presence of threats, blame, and hostility, as well as situational experiences consisting of family, religion, and money. Despite the increase in a few negative situational characteristics in lower HDI countries, the overall average experience still ranged from neutral to slightly positive, rather than negative, suggesting that greater HDI may not necessarily increase positive experiences but rather decrease negative experiences. The results illustrate how national economic status influences the lives of individuals even within a single instance of daily life, with large and powerful consequences when accumulated across individuals within each nation

GERMINAL, a fuel performance code of the PLEIADES platform to simulate the in-pile behaviour of mixed oxide fuel pins for sodium-cooled fast reactors

International audienceGERMINAL is a fuel performance code developed by the French Commission of Alternative and Atomic Energies (CEA) to simulate the in-pile behaviour of mixed oxide fuel pins for Sodium-cooled Fast Reactors. The code is continuously being improved.GERMINAL was initially designed to simulate the fuel pin behaviour of the PHENIX and SUPER-PHENIX reactors, which were built in France and have been in operation over the last decades. The GERMINAL models were then extended and improved to meet the needs of the design studies of ASTRID, a project of a technological Sodium Fast Reactor demonstrator in France. The goal of this article is to introduce the current modelling implemented in GERMINAL. The code is validated and the validation work is illustrated here by a selection of comparisons between calculations and measurements. Working perspectives for further modelling improvements are finally presented, through more mechanistic approaches sustained by three-dimensional computations or based on extended physical couplings

Current status and progression of germinal fuel performance code for sfr oxide fuel pins

International audienceA fuel performance code for SFR oxide fuel pins, GERMINAL, is developed by CEA within the PLEIADES simulation framework. The present main goal of GERMINAL is to meet the needs of the design studies of ASTRID, the future Advanced Sodium Technological Reactor for Industrial Demonstration in France. Recent works have been conducted to improve the modelling of different physical mechanisms having a strong influence on the design criteria evaluation. Thus, the formulation of the fuel pellet fragments relocation model has been revisited, by introducing a dependence to the thermal gradient inside the pellet. The description of this mechanism represents a key point to evaluate the pellet-to-cladding gap closure and the margin to melting at beginning of life. Another evolution concerns the pellet-clad mechanical interaction. The ability to simulate a stronger interaction for fuel pins with a higher filling fraction has been acquired with a focused work on fuel mechanical behavior. A stronger mechanical interaction may also happen with lower power operating conditions and a cladding material remaining stable under irradiation. Moreover, the description of the thermochemistry of oxide fuel is currently being improved by coupling GERMINAL with the OpenCalphad thermodynamic calculation software. In doing this, the goal is to obtain a better prediction of the amount of volatile fission products being transported outside the fuel pellet, and then contributing to the Joint Oxyde-Gaine formation. With refined estimations of JOG volume and composition, we expect further to improve the evaluation of heat transfer through pellet-to-cladding gap at high burn-up, and also a more mechanistic description of cladding corrosion due to released fission products. These works are based on a systematic comparison of calculation results to post-irradiation measures, by integrating progressively additional objects to our validation base. This process leads to a wider validity range targeting ASTRID design, and brings out new working perspectives

3d simulation in the pleiades software environment for sodium fast reactor fuel pin behavior under irradiation

International audienceIn the framework of the basic design of ASTRID (Advanced Sodium Technological Reactor for Industrial Demonstration) the GERMINAL fuel performance code is developed in the PLEIADES software environment. In order to improve one dimensional modelling of GERMINAL, a 3D simulation for the SFR fuel pin behavior under irradiation has been proposed. The 3D model represents a single pellet fragment and its associated piece of cladding. The scale transfer between this single fragment model and the fuel pin scale is achieved through appropriate boundary conditions given by GERMINAL results. The 3D thermo-mechanical computation scheme is implemented in the LICOS code of the PLEIADES platform. In this approach, chemo-physical state variables are pre-computed by the GERMINAL code and are introduced in the 3D computation scheme as some input data in a two-step procedure. First studies have been achieved in order to analyse pellet-to-cladding gap closure mechanisms at the beginning of irradiation. Two mechanisms of fuel relocation have been identified through the 3D simulation. The first one is linked to the hourglass shape of the fragmented fuel pellet under thermal gradient, and the second one is induced by the mass transfer due the central-hole formation and fuel restructuration. Thanks to our 3D results, the gap closure rate given by the fuel relocation displacement model of GERMINAL can be interpreted. The next step is now to propose a full coupling formulation between fuel mass transfer model and the radial relocation displacement model

Why do people come to support a highly controversial law? Manipulating the clarity and coherence of a law can shape individuals’ perception and approval

International audienceIn our research, we investigated the role of law clarity and coherence on individuals’ approvalof this law in the specific context of a widely discussed and controversial topic: the ban onwearing religious signs in public spaces. We hypothesize that manipulating the clarity andcoherence of a law influences individuals’ approval of that law through a change of theirperception. We conducted two experiments in which participants read a fictitious proposedlaw. We manipulated the degree of clarity and coherence of the law and measuredparticipants’ perception of and adherence to that law. The results consistently supported ourhypotheses. Our research contributes to the literature by showing how individuals’ perceptionand approval of a law are malleable, even in the specific context of a hot topic. Furthermore,these findings should sensitize policy makers on how to effectively communicate newpolicies