Design, manufacturing and set-up tests of a wave energy converter prototype in the context of the European Project LIFE-Demowave

LifeDemoWave Project is a real case of development of a R&D project, from the initial idea to the final construction and installation of a prototype for testing in operational environment. This project was born from an idea of the main researcher of the CIMA Group that finally led to two patents of two wave generation systems. Based on these patents, CIMA sought different ways for funding with the aim of developing prototypes with a high TRL and being able to test the operating principle of the patented systems. Finally, funding was obtained through the Life Program of the European Union and in collaboration with five other partners. The main objective of the LifeDemoWave project (http://www. life-demowave.eu/en/) is the demonstration of the feasibility of the use of wave power for electric generation in order to reduce greenhouse gases' emissions. For demonstration purposes, prototypes of wave power generation, reproducible and scalable at high level, are installed in the Galician coast. LifeDemoWave project considers, as well as its design and implementation, the environmental impact in the installation areas and its effect on biodiversity.Peer Reviewe

Ozone Improves the Aromatic Fingerprint of White Grapes

Ozone, a powerful oxidative stressor, has been recently used in wine industry as sanitizing agent to reduce spoilage microflora on grapes. In this study, we evaluated ozone-induced metabolic and molecular responses during postharvest grape dehydration. Ozone increased the contents of total volatile organic compounds (VOCs), which have a great impact on the organoleptic properties of grapes and wines. Among terpenes, responsible for floral and fruity aroma, linalool, geraniol and nerol were the major aromatic markers of Moscato bianco grapes. They were significantly affected by the long-term ozone treatment, increasing their concentration in the last phases of dehydration (>20% weight loss). At molecular level, our results demonstrated that both postharvest dehydration and ozone exposure induce the biosynthesis of monoterpenes via methylerythritol phosphate (MEP) pathway and of aldehydes from lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway. Therefore, transcriptional changes occurred and promoted the over-production of many important volatile compounds for the quality of white grapes

On the Mediterranean Water Composition

The Mediterranean Outflow Water (MOW) spills from the Mediterranean Sea (east North Atlantic basin) west off the Strait of Gibraltar. As MOW outflows, it entrains eastern North Atlantic Central Waters (ENACW) and Intermediate Waters to form the neutrally buoyant Mediterranean Water (MW) that can be traced over the entire North Atlantic basin. Its high salinity content influences the thermohaline properties of the intermediate–deep water column in the North Atlantic and its dynamics. Here, the composition of MW in its source region (the Gulf of Cádiz, west off Strait of Gibraltar) is investigated on the basis of an optimum multiparameter analysis. The results obtained indicate that mixing of MOW (34.1% ± 0.3%) occurs mainly with overlying ENACW (57.1% ± 0.8%) in a process broadly known as central water entrainment. A diluted form (80% of dilution) of the Antarctic Intermediate Water (AAIW) reaches the region and also takes part in MW formation (8.3% ± 0.5%). Finally, the underlying Labrador Sea Water (LSW) also contributes (0.4% ± 0.1%) to the characteristics of MW. From these results and considering 0.74 Sverdrups (Sv; 1 Sv ≡ 106 m3 s−1) as the mean outflow of MOW, the MW exportation rate was inferred (2.2 Sv), which, decomposing MW, means that the MOW outflow is accompanied by 1.24 Sv of entrained ENACW, 0.18 Sv of AAIW, and <0.01 Sv of LSW

The contribution of varietal thiols in the diverse aroma of Italian monovarietal white wines

Thanks to their low odor detection thresholds, free varietal thiols (VTs) play a key role in the primary aroma of wines, to which they confer an intense scent reminiscent of box tree, grapefruit, citrus fruits, passionfruit and cat urine odor. Excluding wines from a few VT-rich grapevine cultivars, VTs appear to be present in most cultivars at trace levels, although a comprehensive dataset is still missing. The low concentration of VTs combined with their high reactivity and matrix complexity make their determination in wines a challenging task. In this research an optimized liquid chromatography - tandem mass spectrometry (LC-MS/MS) method was validated and used for the quantification of 4-methyl-4-sulfanylpentan-2-one (4-MSP), 3-sulfanylhexan-1-ol (3-SH), 3-sulfanylhexyl acetate (3-SHA) and ethyl 3-sulfanylpropionate (E3SP) in 246 samples (vintage 2019) representative of 18 monovarietal Italian white wines. VTs were detected in all cultivars even though higher values of 3-SH were found in Lugana, Müller-Thurgau and Verdicchio cultivars. Müller-Thurgau wines showed the highest level of 4-MSP, that was mainly correlated to the odor descriptors of passionfruit and box tree/cat urine. The VTs composition of Müller-Thurgau was confirmed on a second set of 50 wines from different vintages. From a sensory perspective, the samples of Müller-Thurgau showed the best positive correlations between chemical variables and the odor descriptors thiol note, passion fruit and box tree/cat urine. These notes are significantly related to 4-MSP, suggesting that it could play a relevant olfactory role for the aroma of Müller-Thurgau wines. Sorting analysis allowed to group these wines according to their thiolic characteristics. The chemical variables and the odor descriptors attributable to the thiol notes are important for Müller-Thurgau and Lugana wines, while the contribution of thiol notes was sensorially negligible for the other wines.18openNoMinistry of Education, University and Research (MIUR) under the PRIN 2017 grant (Prot. 2017RXFFRR, CUP code B38D19000120006)Carlin, Silvia; Piergiovanni, Maurizio; Pittari, Elisabetta; Tiziana Lisanti, Maria; Moio, Luigi; Piombino, Paola; Marangon, Matteo; Curioni, Andrea; Rolle, Luca; Rìo Segade, Susana; Versari, Andrea; Ricci, Arianna; Parpinello, Giuseppina Paola; Luzzini, Giovanni; Ugliano, Maurizio; Perenzoni, Daniele; Vrhovsek, Urska; Mattivi, FulvioCarlin, S.; Piergiovanni, M.; Pittari, E.; Tiziana Lisanti, M.; Moio, L.; Piombino, P.; Marangon, M.; Curioni, A.; Rolle, L.; Rìo Segade, S.; Versari, A.; Ricci, A.; Parpinello, G.P.; Luzzini, G.; Ugliano, M.; Perenzoni, D.; Vrhovsek, U.; Mattivi, F

On the determination of the interaction time of GeV neutrinos in large argon gas TPCs

Next-generation megawatt-scale neutrino beams open the way to studying neutrino-nucleus scattering resorting, for the first time, to gaseous targets. This could lead to deeper knowledge of neutrino cross sections in the energy region between hundreds of MeV and a few GeV, of interest for the upcoming generation of long-baseline neutrino oscillation experiments. The challenge is, therefore, to accurately track and (especially) time the particles produced in neutrino interactions in large and seamless volumes down to few-MeV energies. We propose to accomplish this through an optically-read time projection chamber (TPC) filled with high-pressure argon and equipped with both tracking and timing functions. In this work, we present a detailed study of the time-tagging capabilities of such a device, based on end-to-end optical simulations that include the effect of photon propagation, photosensor response, dark-count rate and pulse reconstruction. We show that the neutrino interaction time could be reconstructed from the primary-scintillation signal with a precision in the range 1--2.5~ns ($\sigma$) for point-like deposits with energies down to 5~MeV, and well below 1~ns for minimum-ionizing particle tracks. A discussion on previous limitations towards such a detection technology, and how they can be realistically overcome in the near future thanks to recent developments in the field, is presented (particularly the strong scintillation yields recently reported for Ar/CF$_4$ mixtures). The performance presented in our analysis seems to be well within reach of next-generation neutrino-oscillation experiments through the instrumentation of the proposed TPC with conventional reflective materials and a SiPM carpet behind a transparent cathode

Modelling phenolic and technological maturities of grapes by means of the multivariate relation between organoleptic and physicochemical properties

The ripeness of grapes at the harvest time is one of the most important parameters for obtaining high quality red wines. Traditionally the decision of harvesting is to be taken only after analysing sugar concentration, titratable acidity and pH of the grape juice (technological maturity). However, these parameters only provide information about the pulp ripeness and overlook the real degree of skins and seeds maturities (phenolic maturity). Both maturities, technological and phenolic, are not simultaneously reached, on the contrary they tend to separate depending on several factors: grape variety, cultivar, adverse weather conditions, soil, water availability and cultural practices. Besides, this divergence is increasing as a consequence of the climate change (larger quantities of CO2, less rain, and higher temperatures). 247 samples collected in vineyards representative of the qualified designation of origin Rioja from 2007 to 2011 have been analysed. Samples contain the four grape varieties usual in the elaboration of Rioja wines (‘tempranillo’, ‘garnacha’, ‘mazuelo’ and ‘graciano’). The present study is the first systematic investigation on the maturity of grapes that includes the organoleptic evaluation of the degree of grapes maturity (sugars/acidity maturity, aromatic maturity of the pulp, aromatic maturity of the skins and tannins maturity) together with the values of the physicochemical parameters (probable alcohol degree, total acidity, pH, malic acid, K, total index polyphenolics, anthocyans, absorbances at 420, 520 and 620 nm, colour index and tartaric acid) determined over the same samples. A varimax rotation of the latent variables of a PLS model between the physicochemical variables and the mean of four sensory variables allows identifying both maturities. Besides, the position of the samples in the first plane defines the effect that the different factors exert on both phenolic and technological maturitiesMinisterio de Economía y Competitividad (CTQ2011-26022) and Junta de Castilla y León (BU108A11-2

Absence of Positive Selection on Centromeric Histones in Tetrahymena Suggests Unsuppressed Centromere-Drive in Lineages Lacking Male Meiosis

Centromere-drive is a process where centromeres compete for transmission through asymmetric "female" meiosis for inclusion into the oocyte. In symmetric "male" meiosis, all meiotic products form viable germ cells. Therefore, the primary incentive for centromere-drive, a potential transmission bias, is believed to be missing from male meiosis. In this article, we consider whether male meiosis also bears the primary cost of centromere-drive. Because different taxa carry out different combinations of meiotic programs (symmetric + asymmetric, symmetric only, asymmetric only), it is possible to consider the evolutionary consequences of centromere-drive in the context of these differing systems. Groups with both types of meiosis have large, rapidly evolving centromeric regions, and their centromeric histones (CenH3s) have been shown to evolve under positive selection, suggesting roles as suppressors of centromere-drive. In contrast, taxa with only symmetric male meiosis have shown no evidence of positive selection in their centromeric histones. In this article, we present the first evolutionary analysis of centromeric histones in ciliated protozoans, a group that only undergoes asymmetric "female" meiosis. We find no evidence of positive selection acting on CNA1, the CenH3 of Tetrahymena species. Cytological observations of a panel of Tetrahymena species are consistent with dynamic karyotype evolution in this lineage. Our findings suggest that defects in male meiosis, and not mitosis or female meiosis, are the primary selective force behind centromere-drive suppression. Our study raises the possibility that taxa like ciliates, with only female meiosis, may therefore undergo unsuppressed centromere drive

A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways

Otitis media (OM), inflammation of the middle ear (ME), is a common cause of conductive hearing impairment. Despite the importance of the disease, the aetiology of chronic and recurrent forms of middle ear inflammatory disease remains poorly understood. Studies of the human population suggest that there is a significant genetic component predisposing to the development of chronic OM, although the underlying genes are largely unknown. Using N-ethyl-N-nitrosourea mutagenesis we identified a recessive mouse mutant, edison, that spontaneously develops a conductive hearing loss due to chronic OM. The causal mutation was identified as a missense change, L972P, in the Nischarin (NISCH) gene. edison mice develop a serous or granulocytic effusion, increasingly macrophage and neutrophil rich with age, along with a thickened, inflamed mucoperiosteum. We also identified a second hypomorphic allele, V33A, with only modest increases in auditory thresholds and reduced incidence of OM. NISCH interacts with several proteins, including ITGA5 that is thought to have a role in modulating VEGF-induced angiogenesis and vascularization. We identified a significant genetic interaction between Nisch and Itga5; mice heterozygous for Itga5-null and homozygous for edison mutations display a significantly increased penetrance and severity of chronic OM. In order to understand the pathological mechanisms underlying the OM phenotype, we studied interacting partners to NISCH along with downstream signalling molecules in the middle ear epithelia of edison mouse. Our analysis implicates PAK1 and RAC1, and downstream signalling in LIMK1 and NF-κB pathways in the development of chronic OM

The DUNE far detector vertical drift technology. Technical design report

DUNE is an international experiment dedicated to addressing some of the questions at the forefront of particle physics and astrophysics, including the mystifying preponderance of matter over antimatter in the early universe. The dual-site experiment will employ an intense neutrino beam focused on a near and a far detector as it aims to determine the neutrino mass hierarchy and to make high-precision measurements of the PMNS matrix parameters, including the CP-violating phase. It will also stand ready to observe supernova neutrino bursts, and seeks to observe nucleon decay as a signature of a grand unified theory underlying the standard model. The DUNE far detector implements liquid argon time-projection chamber (LArTPC) technology, and combines the many tens-of-kiloton fiducial mass necessary for rare event searches with the sub-centimeter spatial resolution required to image those events with high precision. The addition of a photon detection system enhances physics capabilities for all DUNE physics drivers and opens prospects for further physics explorations. Given its size, the far detector will be implemented as a set of modules, with LArTPC designs that differ from one another as newer technologies arise. In the vertical drift LArTPC design, a horizontal cathode bisects the detector, creating two stacked drift volumes in which ionization charges drift towards anodes at either the top or bottom. The anodes are composed of perforated PCB layers with conductive strips, enabling reconstruction in 3D. Light-trap-style photon detection modules are placed both on the cryostat's side walls and on the central cathode where they are optically powered. This Technical Design Report describes in detail the technical implementations of each subsystem of this LArTPC that, together with the other far detector modules and the near detector, will enable DUNE to achieve its physics goals