Determinación de NO3 – con electrodo de ión selectivo en aguas de bebida de diversas poblaciones de la provincia de Granada

Se ha detenninado el contenido de ión NO-3 en aguas de bebida en diversas poblaciones de la provincia de Granada, mediante la utilización de un electrodo de ión selectivo de NO3 . Encontrándose que el contenido de NO-3de todas las aguas analizadas están dentro de los límites permitidos en la legislación española para aguas potables que consideran tolerable un máximo de 50 mg/l. Mediante el método de adición simple, los valores calculados para la concentración de NO-3 están comprendidos entre 1,44 mg/l en Lobras y 28,2 mg/l en la Zubia, con un error medio del resultado de ± 0,01 y ± 0,21 respectivamente y los intervalos de confianza son de ± 0,02 y ± 0,51

Kinematic analysis of the Large Magellanic Cloud using Gaia DR3

Context: The high quality of the Gaia mission data is allowing to study the internal kinematics of the Large Magellanic Cloud (LMC) in unprecedented detail, providing insights on the non-axisymmetric structure of its disc. Aims: To define and validate an improved selection strategy to distinguish the LMC stars from the Milky Way foreground. To check the possible biases that assumed parameters or sample contamination from the Milky Way can introduce in the analysis of the internal kinematics of the LMC using Gaia data. Methods: Our selection is based on a supervised Neural Network classifier using as much as of the Gaia DR3 data as possible. We select three samples of candidate LMC stars with different degrees of completeness and purity; we validate them using different test samples and we compare them with the Gaia Collaboration paper sample. We analyse the resulting velocity profiles and maps, and we check how these results change when using also the line-of-sight velocities, available for a subset of stars. Results: The contamination in the samples from Milky Way stars affects basically the results for the outskirts of the LMC, and the absence of line-of-sight velocities does not bias the results for the kinematics in the inner disc. For the first time, we perform a kinematic analysis of the LMC using samples with the full three dimensional velocity information from Gaia DR3. Conclusions: The dynamics in the inner disc is mainly bar dominated; the kinematics on the spiral arm over-density seem to be dominated by an inward motion and a rotation faster than that of the disc in the piece of the arm attached to the bar; contamination of MW stars seem to dominate the outer parts of the disc and mainly affects old evolutionary phases; uncertainties in the assumed disc morphological parameters and line-of-sight velocity of the LMC can in some cases have significant effects. [ABRIDGED

Determinación de NO3 – con electrodo de ión selectivo en aguas de bebida de diversas poblaciones de la provincia de Granada

Se ha detenninado el contenido de ión NO-3 en aguas de bebida en diversas poblaciones de la provincia de Granada, mediante la utilización de un electrodo de ión selectivo de NO3 . Encontrándose que el contenido de NO-3de todas las aguas analizadas están dentro de los límites permitidos en la legislación española para aguas potables que consideran tolerable un máximo de 50 mg/l. Mediante el método de adición simple, los valores calculados para la concentración de NO-3 están comprendidos entre 1,44 mg/l en Lobras y 28,2 mg/l en la Zubia, con un error medio del resultado de ± 0,01 y ± 0,21 respectivamente y los intervalos de confianza son de ± 0,02 y ± 0,51.The NO-3 Ion content in drinkable water taking from various towns of the province of Granada by using a NO-3 ión selective Electrode it has been determined. The NO-3 content in all analyzed water has been found that is inc1uded within the allowed limits according to Spanish legislation for drinkable water where a 50 mg/l maximium is tolerable. The calculated values for the N03 concentration, through the method of addition simple, are comprised between 1,44 mg/l in Lobras and 28,2 mg/l in la Zubia, with an average error in the result of ± 0,01 and ± 0,21 respectively and the creadibility gaps are ± 0,02 and ± 0,51

Striatal expression of GDNF and differential vulnerability of midbrain dopaminergic cells

Glial cell line-derived neurotrophic factor (GDNF) is a member of the transforming growth factor-beta superfamily that when exogenously administrated exerts a potent trophic action on dopaminergic (DA) cells. Although we know a lot about its signalling mechanisms and pharmacological effects, physiological actions of GDNF on the adult brain remain unclear. Here, we have used morphological and molecular techniques, and an experimental model of Parkinson's disease in rats, to investigate whether GDNF constitutively expressed in the adult mesostriatal system plays a neuroprotective role on midbrain DA cells. We found that although all midbrain DA cells express both receptor components of GDNF (GFRalpha1 and Ret), those in the ventral tegmental area (VTA) and rostromedial substantia nigra (SNrm) also contain GDNF but not GDNFmRNA. The levels of GDNFmRNA are significantly higher in the ventral striatum (vSt), the target region of VTA and SNrm cells, than in the dorsal striatum (dSt), the target region of DA cells in the caudoventral substantia nigra (SNcv). After fluoro-gold injection in striatum, VTA and SNrm DA cells show triple labelling for tyrosine hydroxylase, GDNF and fluoro-gold, and after colchicine injection in the lateral ventricle, they become GDNF-immunonegative, suggesting that GDNF in DA somata comes from their striatal target. As DA cells in VTA and SNrm are more resistant than those in SNcv to intracerebroventricular injection of 6-OHDA, as occurs in Parkinson's disease, we can suggest that the fact that they project to vSt, where GDNF expression is significantly higher than in the dSt, is a neuroprotective factor involved in the differential vulnerability of midbrain DA neurons

Adolescents and adults with Fontan circulation:insights from the PREpArE-Fontan registry

The Patient Registry for Adolescents and Adults with Stable Fontan Circulation aims to describe a contemporary cohort of Fontan patients who could be eligible for a clinical trial investigating macitentan, an endothelin receptor antagonist. This international, non-interventional, multicentre, cross-sectional, observational registry enrolled patients with “stable” Fontan circulation ≥10 years following extra-cardiac conduit or lateral tunnel procedure. Main exclusion criteria were NYHA functional class IV, reoperation of Fontan circulation, or signs of disease worsening. Patient characteristics at enrolment are described; available data were collected during a single registration visit. Of the 266 screened patients, 254 were included in this analysis. At enrolment, median (interquartile range) age was 24 (20;30) years, 37%/63% of patients were from the USA/Europe, 54% were male, 54%/47% had undergone extra-cardiac conduit/lateral tunnel procedures, and 95% were in NYHA functional class I or II. History of arrhythmia was more common in older patients and patients with lateral tunnel; overall prevalence was 19%. Most laboratory values were within the normal range but mean creatinine clearance was abnormally low (87.7 ml/min). Angiotensin-converting enzyme inhibitors were used by 48% of patients and their use was associated with creatinine clearance <90 ml/min (p = 0.007), as was Fontan completion at an older age (p = 0.007). 53.4% of patients had clinical characteristics that could potentially meet an endothelin receptor antagonist trial’s eligibility criteria. The PREpArE-Fontan registry describes a cohort of patients who could potentially participate in an endothelin receptor antagonist trial and identified early subtle signs of Fontan failure, even in “stable” patients

Different healthy habits between northern and southern Spanish school children

Aim: Healthy habits are influenced by several factors such as, geographical location. The aims of this study were to describe and compare healthy habits within two populations of sixth-grade primary school children (aged 11-12 years) from Northern and Southern Spain. Subject and Methods: A cross-sectional study using two representative samples of school children was conducted. Participants came from Logroño (n=329), in the North and Granada (n=284), in the South of Spain. Socio-demographic and anthropometric variables, adherence to the Mediterranean diet, aerobic fitness, and healthy lifestyles were recorded. Results: Boys reported higher level of physical activity and aerobic fitness than girls (p=0.000). Southern school children reported significantly higher adherence to the Mediterranean diet (♀: p=0.041; ♂: p=0.008), and lower aerobic fitness (♀: p=0.000; ♂: p=0.042) and hours of nightly sleep (♀: p=0.008, ♂: p=0.007) than Northern school children. Southern boys also reported lower levels of physical activity (p=0.013). There were slight or moderate correlations among all habits measured (physical activity, diet, screen and sleep time). Additionally, physical activity level was inversely related to body mass index in Northern boys (p=0.020) and Southern girls (p=0.024). Conclusions: Results showed differences in physical activity, eating and sleep habits, and aerobic fitness, according to geographical location. The relationships found among lifestyle habits indicate the need of health promotion interventions nationally and considering the differences discussed here

Serum Lipopolysaccharide Binding Protein Levels Predict Severity of Lung Injury and Mortality in Patients with Severe Sepsis

Background: There is a need for biomarkers insuring identification of septic patients at high-risk for death. We performed a prospective, multicenter, observational study to investigate the time-course of lipopolysaccharide binding protein (LBP) serum levels in patients with severe sepsis and examined whether serial serum levels of LBP could be used as a marker of outcome. Methodology/Principal Findings: LBP serum levels at study entry, at 48 hours and at day-7 were measured in 180 patients with severe sepsis. Data regarding the nature of infections, disease severity, development of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), and intensive care unit (ICU) outcome were recorded. LBP serum levels were similar in survivors and non-survivors at study entry (117.4±75.7 µg/mL vs. 129.8±71.3 µg/mL, P = 0.249) but there were significant differences at 48 hours (77.2±57.0 vs. 121.2±73.4 µg/mL, P<0.0001) and at day-7 (64.7±45.8 vs. 89.7±61.1 µg/ml, p = 0.017). At 48 hours, LBP levels were significantly higher in ARDS patients than in ALI patients (112.5±71.8 µg/ml vs. 76.6±55.9 µg/ml, P = 0.0001). An increase of LBP levels at 48 hours was associated with higher mortality (odds ratio 3.97; 95%CI: 1.84–8.56; P<0.001). Conclusions/Significance: Serial LBP serum measurements may offer a clinically useful biomarker for identification of patients with severe sepsis having the worst outcomes and the highest probability of developing sepsis-induced ARDS

University–industry collaboration: using meta-rules to overcome barriers to knowledge transfer

This is the final version of the article. Available from Springer Verlag via the DOI in this record.University–industry knowledge transfer is an important source wealth of creation for all partners; however, the practical management of this activity within universities is often hampered by procedural rigidity either through the absence of decision-making protocols to reconcile conflicting priorities or through the inconsistent implementation of existing policies. This is problematic, since it can impede operational effectiveness, prevent inter-organisational knowledge-creation and hamper organisational learning. This paper addresses this issue by adopting a cross-discipline approach and presenting meta-rules as a solution to aid organisational decision making. It is proposed that meta-rules can help resolve tensions arising from conflicting priorities between academics, knowledge transfer offices and industry and help facilitate strategic alignment of processes and policies within and between organisations. This research contributes to the growing debate on the strategic challenges of managing knowledge transfer and presents meta-rules as a practical solution to facilitate strategic alignment of internal and external stakeholder tensions. Meta-rules has previously only been applied in a computer intelligence context however, this research proves the efficacy of meta rules in a university–industry knowledge transfer context. This research also has practical implications for knowledge transfer office managers who can use meta-rules to help overcome resource limitations, conflicting priorities and goals of diverse internal and external stakeholders