197 research outputs found

    Utilización del chapulixtle (Dodonae viscosa) en los procesos agrícolas de la comunidad de Progreso Hidalgo, Estado de México

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    Progreso Hidalgo es una comunidad ru ral ubicada en una zona de transición ecológica del Estado de México que se dis tingue por la amplia diversidad de paisajes que, de acuerdo a las condiciones fisiográficas, ambientales y ecológicas, presentan características que explican y favorecen la agrodiversidad y el manejo de los recursos naturales, principalmente de la vegetación silvestre, como es el caso de Dodonae viscosa. El uso de esta planta ha contribuido significativamente en la economía de las familias campesinas, ya que es utilizada en la elaboración de tutores para cultivos de riego con fines comerciales como tomate, zarzamora, chile y jitomate. Las condiciones ambientales, geográficas y ecológicas presentes en Progreso Hidalgo favorecen la existencia de Dodonae viscosa en las laderas, lomeríos y barrancas, y al mismo tiempo su utilización en la agricultura. En este artículo se muestra, mediante entrevistas aplicadas a 60 campesinos de la comunidad, la importancia de esta planta para la elaboración de diversas herramientas utilizadas en la vida diaria de sus pobladores

    RNY3 modulates cell proliferation and IL13 mRNA levels in a T lymphocyte model: a possible new epigenetic mechanism of IL-13 regulation

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    [EN] llergic asthma is the most common type of asthma. It is characterized by TH2 cell–driven infammation in which interleukin-13 (IL-13) plays a pivotal role. Cytoplasmic RNAs (Y-RNAs), a variety of non-coding RNAs that are dysregulated in many cancer types, are also diferentially expressed in patients with allergic asthma. Their function in the development of the disease is still unknown. We investigated the potential role of RNY3 RNA (hY3) in the TH2 cell infammatory response using the Jurkat cell line as a model. hY3 expression levels were modulated to mimic the upregulation efect in allergic disease. We evaluated the efect of hY3 over cell stimulation and the expression of the TH2 cytokine IL13. Total RNA was isolated and retrotranscribed, and RNA levels were assessed by qPCR. In Jurkat cells, hY3 levels increased upon stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. When transfecting with high levels of hY3 mimic molecules, cell proliferation rate decreased while IL13 mRNA levels increased upon stimulation compared to stimulated control cells. Our results show the efect of increased hY3 levels on cell proliferation and the levels of IL13 mRNA in Jurkat cells. Also, we showed that hY3 could act over other cells via exosomes. This study opens up new ways to study the potential regulatory function of hY3 over IL-13 production and its implications for asthma development.Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLE

    Seagrass Halodule wrightii as a new habitat for the amphioxus Branchiostoma californiense (Cephalochordata, Branchiostomidae) in the southern Gulf of California, Mexico

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    The first record of the amphioxus Branchiostoma californiense on seagrass patches of Halodule wrightii in the Gulf of California is reported. Sixty individuals (19 males, 18 females, and 23 undifferentiated) were collected in May 2017 at Bahía Balandra, Gulf of California, from subtidal seagrass patches at a depth of 0.5 m at low tide. The length and weight ranged from 15.88–28.44 mm and from 0.01–0.11 g for females and 11.7–27.9 mm and 0.01–0.09 g for males, respectively. The minimum size of sexually mature individuals was 11.70 mm for males and 15.88 mm for females; 62% of the specimens were sexually mature. Analysis of the total length-weight relationship suggested an allometric growth pattern among females, males and undifferentiated individuals, whereas an analysis of the entire sample suggested an isometric growth pattern. Typical and additional morphological characters were used to identify the amphioxi. High morphological variability between individuals was found, suggesting the presence of several morphotypes. Branchiostoma californiense had been previously reported as exclusively associated with bare sandy areas, but our study shows that this species can also be found in seagrass patches, using them as breeding and feeding grounds. Thus, seagrass patches are evidenced as suitable habitats for amphioxus

    Alkalinity determination by potentiometry - intercalibration using three different methods

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    Seawater was sampled from different depths in the North Atlantic Ocean (Canary Islands region) and distributed among three different labs for the determination of titration alkalinity. Analysis was performed by potentiometric methods, involving titration in a closed cell, titration in an open cell and a two end-point acid addition method. The precision, which is the sample reproducibility taken from the mean standard deviation for replicate measurements, was between 0.45 and 0.90 µmol kg(-1) for the individual labs. Accuracy, here taken as the deviation for the values of a lab from the mean of all three, was mostly below 1 µmol kg(-1) and never exceeded 0.1% of the sample value. Mean standard deviation for all labs and all samples was 0.87 µmol kg(-1), once the individual methods were calibrated using certified reference material (CRM). Without CRM calibration, the mean standard deviation would increase to 2.8 µmol k(-1). The conclusion is that current high precision methods for alkalinity measurements calibrated with CRMs are able to reach similar accuracy as the measurement of total dissolved inorganic carbon by coulometry and therefore allow for the precise determination of the oceanic carbon dioxide system by using the two measured parameters

    Historical Reconstruction of Subordination in a Local Context: Agroecosystems with Sugarcane in Veracruz, Mexico

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    Objective: Rebuild the historical transformation of agroecosystems based on sugarcane in the sub-humid tropic, as a consequence of the change in public policies of the Mexican State in the last three decades. Design/methodology/approach: The transformation of sugar cane agroecosystems was studied in the continuum of reality, as changes in their resource management and management practices. Thus, methodologies used included life stories, field diary, and documentary review. The information collected was transcribed and classified in a database, using keywords for their previous identification by categories. The statements were identified and ordered based on their content, interpretation, and underlying concepts. Results: During the last century, public policies directed towards the sugar sector encouraged the existence of sugarcane agroecosystems as a monoculture. This implied changes in the management of time, work organization, orientation of the production, dependence on credits driven by the mills, and a process encouraged by the individualization of production, eroding the collective and solidarity work that lay behind other crops. Study limitations/implications: It is important to compare these findings with other investigations done in sugar cane producing municipalities. Findings / conclusion: Neoliberal public policies aiming the sugar sector deepened the producers' financial dependence on mills; as a consequence, they lost their autonomy in the management and use of their agroecosystems.Objective: Rebuild the historical transformation of agroecosystems based on sugarcane in the sub-humid tropic, as a consequence of the change in public policies of the Mexican State in the last three decades. Design/methodology/approach: The transformation of sugar cane agroecosystems was studied in the continuum of reality, as changes in their resource management and management practices. Thus, methodologies used included life stories, field diary, and documentary review. The information collected was transcribed and classified in a database, using keywords for their previous identification by categories. The statements were identified and ordered based on their content, interpretation, and underlying concepts. Results: During the last century, public policies directed towards the sugar sector encouraged the existence of sugarcane agroecosystems as a monoculture. This implied changes in the management of time, work organization, orientation of the production, dependence on credits driven by the mills, and a process encouraged by the individualization of production, eroding the collective and solidarity work that lay behind other crops. Study limitations/implications: It is important to compare these findings with other investigations done in sugar cane producing municipalities. Findings / conclusion: Neoliberal public policies aiming the sugar sector deepened the producers' financial dependence on mills; as a consequence, they lost their autonomy in the management and use of their agroecosystems
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