Analysis of QUIC Session Establishment and its Implementations

International audienceIn the recent years, the major web companies have been working to improve the user experience and to secure the communications between their users and the services they provide. QUIC is such an initiative, and it is currently being designed by the IETF. In a nutshell, QUIC originally intended to merge features from TCP/SCTP, TLS 1.3 and HTTP/2 into one big protocol. The current specification proposes a more modular definition, where each feature (transport, cryptography, application, packet reemission) are defined in separate internet drafts. We studied the QUIC internet drafts related to the transport and cryptographic layers, from version 18 to version 23, and focused on the connection establishment with existing implementations. We propose a first implementation of QUIC connection establishment using Scapy, which allowed us to forge a critical opinion of the current specification, with a special focus on the induced difficulties in the implementation. With our simple stack, we also tested the behaviour of the existing implementations with regards to security-related constraints (explicit or implicit) from the internet drafts. This gives us an interesting view of the state of QUIC implementations

A fascinating multifaceted redox-active chelating ligand: introducing the N,N′-dimethyl-3,3′-biquinoxalinium “methylbiquinoxen” platform

To intimately combine a chelating ligand function with the numerous properties of a viologen-like redox-active centre would offer a rare possibility to design controllable multi-redox states, whose properties arise from strongly correlated phenomena between the organic ligand as well as with any metalloid coordinated centres. Such a concept previously proved to be feasible, however is not widely applicable owing to challenges in terms of synthesis, isolation, and aerial sensitivity of both the ligand and its metal complexes. Here we report the first stable example of such a redox-active molecule, N,N′-dimethyl-3,3′-biquinoxalinium2+/˙+/0 “methylbiquinoxen, MBqn2+/˙+/0”, which shows a rich redox chemistry and chelates a metal ion in the case of the metal complex [CdCl2(MBqn0)]. This goes beyond what is possible to achieve using viologens, which are limited by not providing chelation as well as having no accessible biradicaloid state, corresponding to the neutral direduced MBqn0 open-shell behaviour we observe here

concerto: A Methodology Towards Reproducible Analyses of TLS Datasets

Over the years, SSL/TLS has become an essential part of Internet security. As such, it should offer robust and state-of-the-art security, in particular for HTTPS, its first application. Theoretically, the protocol allows for a trade-off between secure algorithms and decent performance. Yet in practice, servers do not always support the latest version of the protocol, nor do they all enforce strong cryptographic algorithms. To assess the quality of HTTPS and other TLS deployment at large, several studies have been led to grasp the state of the ecosystem, and to characterize the quality of certificate chains in particular. In this paper, we propose to analyse some of the existing data concerning TLS measures on the Internet. We studied several datasets, from the first public ones in 2010 to more recent scans. Even if the collection methodology and the used tools vary between campaigns, we propose a unified and reproducible way to analyse the TLS ecosystem through different datasets. Our approach is based on a set of open-source tools, concerto. Our contribution is therefore threefold: an analysis of existing datasets to propose a unified methodology, the implementation of our approach with concerto, and the presentation of some results to validate our toolsets

The Host Microbiota Contributes to Early Protection Against Lung Colonization by Mycobacterium tuberculosis

Tuberculosis (TB), caused by the airborne bacterial pathogen Mycobacterium tuberculosis, remains a major source of morbidity and mortality worldwide. So far, the study of host-pathogen interactions in TB has mostly focused on the physiology and virulence of the pathogen, as well as, on the various innate and adaptive immune compartments of the host. Microbial organisms endogenous to our body, the so-called microbiota, interact not only with invading pathogens, but also with our immune system. Yet, the impact of the microbiota on host defense against M. tuberculosis remains poorly understood. In order to address this question, we adapted a robust and reproducible mouse model of microbial dysbiosis based on a combination of wide-spectrum antibiotics. We found that microbiota dysbiosis resulted in an increased early colonization of the lungs by M. tuberculosis during the first week of infection, correlating with an altered diversity of the gut microbiota during this time period. At the cellular level, no significant difference in the recruitment of conventional myeloid cells, including macrophages, dendritic cells and neutrophils, to the lungs could be detected during the first week of infection between microbiota-competent and -deficient mice. At the molecular level, microbiota depletion did not impact the global production of pro-inflammatory cytokines, such as interferon (IFN)γ, tumor necrosis factor (TNF)α and interleukin (IL)-1β in the lungs. Strikingly, a reduced number of mucosal-associated invariant T (MAIT) cells, a population of innate-like lymphocytes whose development is known to depend on the host microbiota, was observed in the lungs of the antibiotics-treated animals after 1week of infection. These cells produced less IL-17A in antibiotics-treated mice. Notably, dysbiosis correction through the inoculation of a complex microbiota in antibiotics-treated animals reversed these phenotypes and improved the ability of MAIT cells to proliferate. Altogether, our results demonstrate that the host microbiota contributes to early protection of lung colonization by M. tuberculosis, possibly through sustaining the function(s) of MAIT cells. Our study calls for a better understanding of the impact of the microbiota on host-pathogen interactions in TB. Ultimately, this study may help to develop novel therapeutic approaches based on the use of beneficial microbes, or components thereof, to boost anti-mycobacterial immunity

Macrophage Plasticity in Experimental Atherosclerosis

As in human disease, macrophages (MØ) are central players in the development and progression of experimental atherosclerosis. In this study we have evaluated the phenotype of MØ associated with progression of atherosclerosis in the apolipoprotein E (ApoE) knockout (KO) mouse model

Preclinical assessment of a new live attenuated Mycobacterium tuberculosis Beijing-based vaccine for tuberculosis.

Tuberculosis still claims more lives than any other pathogen, and a vaccine better than BCG is urgently needed. One of the challenges for novel TB vaccines is to protect against all Mycobacterium tuberculosis lineages, including the most virulent ones, such as the Beijing lineage. Here we developed a live attenuated M. tuberculosis mutant derived from GC1237, a Beijing strain responsible for tuberculosis outbreaks in the Canary Islands. The mutant strain is inactivated both in the Rv1503c gene, responsible for surface glycolipid synthesis, and in the two-component global regulator PhoPR. This double mutant is as safe as BCG in immunodeficient SCID mice. In immune-competent mice and guinea pigs, the mutant is as protective as BCG against M. tuberculosis strains of common lineage 4 (Euro-American). By contrast, in mice the vaccine is protective against a M. tuberculosis strain of lineage 2 (East-Asian, Beijing), while BCG is not. These results highlight differences in protection efficacy of live attenuated M. tuberculosis-derived vaccine candidates depending on their genetic background, and provide insights for the development of novel live vaccines against TB, especially in East-Asian countries where M. tuberculosis strains of the Beijing family are highly dominant

Estimation of π-π Electronic Couplings from Current Measurements

The - interactions between organic molecules are among the most important parameters for optimizing the transport and optical properties of organic transistors, light-emitting diodes, and (bio-)molecular devices. Despite substantial theoretical progress, direct experimental measurement of the - electronic coupling energy parameter t has remained an old challenge due to molecular structural variability and the large number of parameters that affect charge transport. Here, we propose a study of - interactions from electrochemical and current measurements on a large array of ferrocene-thiolated gold nanocrystals. We confirm the theoretical prediction that t can be assessed from a statistical analysis of current histograms. The extracted value of t ≈ 35 meV is in the expected range based on our density functional theory analysis. Furthermore, the t distribution is not necessarily Gaussian and could be used as an ultrasensitive technique to assess intermolecular distance fluctuation at the sub-angstrom level. The present work establishes a direct bridge between quantum chemistry, electrochemistry, organic electronics, and mesoscopic physics, all of which were used to discuss results and perspectives in a quantitative manner