Papiloma virus humano y neoplasia cervical: relación histopatológica. Hospital Regional Teodoro Maldonado Carbo, 1999-2000

Se realizó un estudio de tipo analítico, transversal de caracter retrospectivo, no aleatorio, monocéntrico, en el hospital Teodoro Maldonado Carbo; cuyo objetivo principal fue demostrar el grado de asociación de la infección por Papiloma virus humano y neoplasia cervical en pacientes que acudieron al hospital durante los años 1999 y 2000. El resultado principal fue la demostración de la relación entre la infección por Papiloma virus humano y la neoplasia cervical, siendo la población urbana más afectada y su mayor incidencia en la cuarta década de la vida.Conclusiones: se demostró por medio del trabajo la alta asociación de la enfermedad en relación a la infección, siendo el riesgo relativo de 3.9, y la ocurrencia relativa de 5.8

How glycobiology can help us treat and beat the COVID-19 pandemic

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged during the last months of 2019, spreading throughout the world as a highly transmissible infectious illness designated as COVID-19. Vaccines have now appeared, but the challenges in producing sufficient material and distributing them around the world means that effective treatments to limit infection and improve recovery are still urgently needed. This review focuses on the relevance of different glycobiological molecules that could potentially serve as or inspire therapeutic tools during SARS-CoV-2 infection. As such, we highlight the glycobiology of the SARS-CoV-2 infection process, where glycans on viral proteins and on host glycosaminoglycans have critical roles in efficient infection. We also take notice of the glycan-binding proteins involved in the infective capacity of virus and in human defense. In addition, we critically evaluate the glycobiological contribution of candidate drugs for COVID-19 therapy such as glycans for vaccines, anti-glycan antibodies, recombinant lectins, lectin inhibitors, glycosidase inhibitors, polysaccharides, and numerous glycosides, emphasizing some opportunities to repurpose FDA-approved drugs. For the next-generation drugs suggested here, biotechnological engineering of new probes to block the SARS-CoV-2 infection might be based on the essential glycobiological insight on glycosyltransferases, glycans, glycan-binding proteins, and glycosidases related to this pathology.publishedVersionFil: Lardone, Ricardo Dante. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Lardone, Ricardo Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina.Fil: Garay, Yohana Camila. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Garay, Yohana Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina.Fil: Parodi, Pedro. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Parodi, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina.Fil: de la Fuente, Sofia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: de la Fuente, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina.Fil: Angeloni, Genaro. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Angeloni, Genaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina.Fil: Bravo, Eduardo O. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos San Roque de Gonnet; ArgentinaFil: Schmider, Anneke K. Psychiatrische Klinik Lüneburg; AlemaniaFil: Irazoqui, Fernando José. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Irazoqui, Fernando José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina

Close companions around young stars

Multiplicity is a fundamental property that is set early during stellar lifetimes, and it is a stringent probe of the physics of star formation. The distribution of close companions around young stars is still poorly constrained by observations. We present an analysis of stellar multiplicity derived from APOGEE-2 spectra obtained in targeted observations of nearby star-forming regions. This is the largest homogeneously observed sample of high-resolution spectra of young stars. We developed an autonomous method to identify double lined spectroscopic binaries (SB2s). Out of 5007 sources spanning the mass range of $\sim$0.05--1.5 \msun, we find 399 binaries, including both RV variables and SB2s. The mass ratio distribution of SB2s is consistent with a uniform for $q0.95$. The period distribution is consistent with what has been observed in close binaries ($<10$ AU) in the evolved populations. Three systems are found to have $q\sim$0.1, with a companion located within the brown dwarf desert. There are not any strong trends in the multiplicity fraction (MF) as a function of cluster age from 1 to 100 Myr. There is a weak dependence on stellar density, with companions being most numerous at $\Sigma_*\sim30$ stars/pc$^{-2}$, and decreasing in more diffuse regions. Finally, disk-bearing sources are deficient in SB2s (but not RV variables) by a factor of $\sim$2; this deficit is recovered by the systems without disks. This may indicate a quick dispersal of disk material in short-period equal mass systems that is less effective in binaries with lower $q$.Comment: 25 pages, 20 figures. Accepted to A

Corantes como fontes de pigmentação para gemas de ovos de galinhas

A comparison was made of the egg yolk pigmentation produced by two comercial products, Carophyll red (10% canthaxantin) and Pigmenting Complex 1131 (streptoxanthin). The colour of ten yolks a day per treatment was determined over an experimental period of 20 days using a Roche Colour Fan and a Hunter Lab Colour & Colour Difference Meter. The results show that pigment deposition first appeared in the yolks of eggs laid on the 5th day of treatment. Both pigmenting sources were significantly different at the 1% level from the control group but there was no difference between them. The correlation between the two methods of measurement was highly significant.Foi comparado o comportamento da estreptoxantina, produto novo no mercado, com "Carophyll" vermelho, já usado comercialmente no Brasil para coloração de gemas de ovos. Foram utilizadas galinhas Leghorn Branca com 14 meses de idade, alojadas a duas por gaiola; o delineamento experimental foi inteiramente casualizado, sendo três tratamentos com quatro repetições, constituindo 20 aves cada parcela. A determinação diária da cor foi feita em gemas de dez ovos de cada parcela, nos períodos pré-experimental (10 dias), experimental (20 dias) e pós-experimental (até que a coloração retornasse ao normal). A avaliação subjetiva da cor foi feita pelo leque colorido da Roche, dando os resultados por uma escala de 1 a 15 pontos, e a objetiva, pelo colorímetro diferencial Hunter, que expressa os resultados através de três parâmetros. Os resultados mostraram que o efeito dos corantes apareceu a partir do quinto dia do inicio da sua adição e permaneceu por seis a sete dias após sua suspensão; houve diferença significativa ao nível de significância de 1% entre a testemunha e os dois tratamentos com corantes, não ocorrendo, entretanto, diferença significativa entre estes. Os métodos utilizados para determinação da cor em gemas do ovos estão altamente correlacionados

Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A

Enterotoxigenic Escherichia coli (ETEC) is a major cause of morbidity in children under 5 years of age in low- and middle-income countries and a leading cause of traveler's diarrhea worldwide. The ability of ETEC to colonize the intestinal epithelium is mediated by fimbrial adhesins, such as CS21 (Longus). This adhesin is a type IVb pilus involved in adherence to intestinal cells in vitro and bacterial self-aggregation. Fourteen open reading frames have been proposed to be involved in CS21 assembly, hitherto only the lngA and lngB genes, coding for the major (LngA) and minor (LngB) structural subunit, have been characterized. In this study, we investigated the role of the LngA, LngB, LngC, LngD, LngH, and LngP proteins in the assembly of CS21 in ETEC strain E9034A. The deletion of the lngA, lngB, lngC, lngD, lngH, or lngP genes, abolished CS21 assembly in ETEC strain E9034A and adherence to HT-29 cells was reduced 90%, compared to wild-type strain. Subcellular localization prediction of CS21 proteins was similar to other well-known type IV pili homologues. We showed that LngP is the prepilin peptidase of LngA, and that ETEC strain E9034A has another peptidase capable of processing LngA, although with less efficiency. Additionally, we present immuno-electron microscopy imagens to show that the LngB protein could be localized at the tip of CS21, and probably helps to control CS21 length. In conclusion, our results demonstrate that the LngA, LngB, LngC, LngD, LngH, and LngP proteins are essential for CS21 assembly, as well as for bacterial aggregation and adherence to HT-29 cells

The Fourteenth Data Release of the Sloan Digital Sky Survey: First Spectroscopic Data from the extended Baryon Oscillation Spectroscopic Survey and from the second phase of the Apache Point Observatory Galactic Evolution Experiment

The fourth generation of the Sloan Digital Sky Survey (SDSS-IV) has been in operation since July 2014. This paper describes the second data release from this phase, and the fourteenth from SDSS overall (making this, Data Release Fourteen or DR14). This release makes public data taken by SDSS-IV in its first two years of operation (July 2014-2016). Like all previous SDSS releases, DR14 is cumulative, including the most recent reductions and calibrations of all data taken by SDSS since the first phase began operations in 2000. New in DR14 is the first public release of data from the extended Baryon Oscillation Spectroscopic Survey (eBOSS); the first data from the second phase of the Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE-2), including stellar parameter estimates from an innovative data driven machine learning algorithm known as "The Cannon"; and almost twice as many data cubes from the Mapping Nearby Galaxies at APO (MaNGA) survey as were in the previous release (N = 2812 in total). This paper describes the location and format of the publicly available data from SDSS-IV surveys. We provide references to the important technical papers describing how these data have been taken (both targeting and observation details) and processed for scientific use. The SDSS website (www.sdss.org) has been updated for this release, and provides links to data downloads, as well as tutorials and examples of data use. SDSS-IV is planning to continue to collect astronomical data until 2020, and will be followed by SDSS-V.Comment: SDSS-IV collaboration alphabetical author data release paper. DR14 happened on 31st July 2017. 19 pages, 5 figures. Accepted by ApJS on 28th Nov 2017 (this is the "post-print" and "post-proofs" version; minor corrections only from v1, and most of errors found in proofs corrected