There be giants! The importance of taxonomic clarity of the large ocean sunfishes (genus Mola, Family Molidae) for assessing sunfish vulnerability to anthropogenic pressures.

The study of ocean sunfishes has for decades been practically synonymous with the study of Mola mola, recently listed as ‘vulnerable’ on a global scale by the International Union for Conservation of Nature. The concerns are high levels of fisheries bycatch worldwide, however sunfish bycatch is rarely identified to species level anywhere, perhaps as a long legacy of taxonomic confusion in the sunfish taxonomy has rendered identification to species level challenging. This includes the Australian and New Zealand longline fisheries, where sunfishes are listed at “High Risk” due to data deficiency. In the popular sunfish SCUBA dive tourism off Bali, Indonesia, another type of anthropogenic pressure is manifested through diver crowding, preventing sunfish from interacting with cleaner-fish on the local reefs. The consequences are difficult to gauge due to a paucity of information on this highly seasonal phenomenon, but have motivated discussions of sunfish protection by Indonesian authorities. In this study, the species identities and zoogeographies of the little studied sunfishes in Australia and New Zealand were explored through biopsy sampling in the longline fisheries, and by reviewing museum collections across both countries. Specimen IDs were established phylogenetically and/or morphologically. Combined, the results revealed a new species of ocean sunfish, Mola tecta, which was diagnosed and described. Furthermore, the results showed that three large species of sunfish dominate the tropical, subtropical/warmtemperate and cold-temperate waters of Australia and New Zealand; Masturus lanceolatus, Mola alexandrini and Mola tecta, respectively. Mola mola appears to be rare. These results imply that the long-term fisheries observer sunfish bycatch data from both countries consists of a mix of species. Bycatch rate analyses within four fishing grounds sub-areas, each presumably dominated by one species of sunfish, did not reveal downwards trends over the 10 - 12 year periods, for which data were available. In the Bali tourism industry, the sunfish species identity was confirmed molecularly and morphologically as Mola alexandrini. The strong seasonality in diver sightings was documented through sunfish encounter rates based on operator logs. The results revealed that the 2015 sunfish season (August – October/November) lagged by ~1 month the seasonal change in intensity of two major oceanographic features of the area, established from in situ and satellite sea surface temperature data. Data from eight satellite tagged sunfish revealed a high affinity by four fish to the dynamic Lombok Straight during the sunfish season, however, at least one other fish left the area. Overall, temperature emerged as an unlikely main driver of the sunfish seasonality, which is instead presumably driven by an increase in sunfish prey availability, associated with the seasonal cold-water upwelling. Overall, these findings have provided much needed clarity to the taxonomy and phylogenetic relationships of the genus Mola, and demonstrated the need for species-level investigations of fisheries bycatch globally to inform fisheries risk assessments. Furthermore, the results provided important information on the sunfish seasonality off Bali to inform tourism management decisions

Polygenic Risk Score Prediction for Endometriosis

Endometriosis is a major health care challenge because many young women with endometriosis go undetected for an extended period, which may lead to pain sensitization. Clinical tools to better identify candidates for laparoscopy-guided diagnosis are urgently needed. Since endometriosis has a strong genetic component, there is a growing interest in using genetics as part of the clinical risk assessment. The aim of this work was to investigate the discriminative ability of a polygenic risk score (PRS) for endometriosis using three different cohorts: surgically confirmed cases from the Western Danish endometriosis referral Center (249 cases, 348 controls), cases identified from the Danish Twin Registry (DTR) based on ICD-10 codes from the National Patient Registry (140 cases, 316 controls), and replication analysis in the UK Biobank (2,967 cases, 256,222 controls). Patients with adenomyosis from the DTR (25 cases) and from the UK Biobank (1,883 cases) were included for comparison. The PRS was derived from 14 genetic variants identified in a published genome-wide association study with more than 17,000 cases. The PRS was associated with endometriosis in surgically confirmed cases [odds ratio (OR) = 1.59, p = 2.57× 10(−7)] and in cases from the DTR biobank (OR = 1.50, p = 0.0001). Combining the two Danish cohorts, each standard deviation increase in PRS was associated with endometriosis (OR = 1.57, p = 2.5× 10(−11)), as well as the major subtypes of endometriosis; ovarian (OR = 1.72, p = 6.7× 10(−5)), infiltrating (OR = 1.66, p = 2.7× 10(−9)), and peritoneal (OR = 1.51, p = 2.6 × 10(−3)). These findings were replicated in the UK Biobank with a much larger sample size (OR = 1.28, p < 2.2× 10(−16)). The PRS was not associated with adenomyosis, suggesting that adenomyosis is not driven by the same genetic risk variants as endometriosis. Our results suggest that a PRS captures an increased risk of all types of endometriosis rather than an increased risk for endometriosis in specific locations. Although the discriminative accuracy is not yet sufficient as a stand-alone clinical utility, our data demonstrate that genetics risk variants in form of a simple PRS may add significant new discriminatory value. We suggest that an endometriosis PRS in combination with classical clinical risk factors and symptoms could be an important step in developing an urgently needed endometriosis risk stratification tool

Identification of common genetic risk variants for autism spectrum disorder

Autism spectrum disorder (ASD) is a highly heritable and heterogeneous group of neurodevelopmental phenotypes diagnosed in more than 1% of children. Common genetic variants contribute substantially to ASD susceptibility, but to date no individual variants have been robustly associated with ASD. With a marked sample-size increase from a unique Danish population resource, we report a genome-wide association meta-analysis of 18,381 individuals with ASD and 27,969 controls that identified five genome-wide-significant loci. Leveraging GWAS results from three phenotypes with significantly overlapping genetic architectures (schizophrenia, major depression, and educational attainment), we identified seven additional loci shared with other traits at equally strict significance levels. Dissecting the polygenic architecture, we found both quantitative and qualitative polygenic heterogeneity across ASD subtypes. These results highlight biological insights, particularly relating to neuronal function and corticogenesis, and establish that GWAS performed at scale will be much more productive in the near term in ASD

Genomic Relationships, Novel Loci, and Pleiotropic Mechanisms across Eight Psychiatric Disorders

Genetic influences on psychiatric disorders transcend diagnostic boundaries, suggesting substantial pleiotropy of contributing loci. However, the nature and mechanisms of these pleiotropic effects remain unclear. We performed analyses of 232,964 cases and 494,162 controls from genome-wide studies of anorexia nervosa, attention-deficit/hyper-activity disorder, autism spectrum disorder, bipolar disorder, major depression, obsessive-compulsive disorder, schizophrenia, and Tourette syndrome. Genetic correlation analyses revealed a meaningful structure within the eight disorders, identifying three groups of inter-related disorders. Meta-analysis across these eight disorders detected 109 loci associated with at least two psychiatric disorders, including 23 loci with pleiotropic effects on four or more disorders and 11 loci with antagonistic effects on multiple disorders. The pleiotropic loci are located within genes that show heightened expression in the brain throughout the lifespan, beginning prenatally in the second trimester, and play prominent roles in neurodevelopmental processes. These findings have important implications for psychiatric nosology, drug development, and risk prediction.Peer reviewe

Species identification of sunfish specimens (Genera Mola and Masturus, Family Molidae) from Australian and New Zealand natural history museum collections and other local sources

This data-in-brief comprises a summary of sunfish specimens (Genera Mola and Masturus, Family Molidae, >29 cm total length) from natural history museum collections and other sources, such as strandings, in Australia and New Zealand. Each specimen was evaluated morphologically and identified to lowest possible taxon based on recent advances in the understanding of the Mola taxonomy. References to phylogenetic analyses, where applicable, are included. The summary was collated in support of publication Giant jelly eaters on the line: species distribution and bycatch of three dominant sunfishes in the Southwest Pacific [1]

Reproductive biology of the loliginid squid, Alloteuthis subulata in the north-east Atlantic and adjacent waters

A study of the reproductive biology of the loliginid squid, Alloteuthis subulata in the North Sea, Irish Sea and Portuguese waters was carried out. A predominance of small squid (<50 mm ML) during autumn was observed in all three areas. Multi-modal size-frequency distributions were apparent in both sexes. The greatest complexity was observed in Portuguese waters, with males exhibiting possibly four or more modal size classes. Sexually mature squid were found throughout the year, with generally more mature squid in spring-summer and fewer in autumn-winter. In Portuguese waters, maturity in both sexes peaked in spring, whereas maturity in the other areas peaked in summer. Similar sizes-at-maturity were observed, with male squid maturing over a greater size range (40-125 mm ML) than females (30-50 mm ML). An apparent secondary peak in male maturity at 65 mm ML suggests two different size-maturation patterns in Portuguese waters. Estimates of potential fecundity in the Irish Sea ranged from similar to 2200-13 500 eggs per female. Mean egg diameters of 0.45 mm and 1.55 mm were recorded for developing oocytes and mature ova, respectively. Three to four modal size classes of oocytes were apparent in each ovary. There was some indication of latitudinal effects on growth, maturation and reproduction of A. subulata across its geographic range. The greater complexity of size-structure in warmer waters suggests that water temperature may be the main factor involved, possibly through shorter incubation times, faster growth and maturation rates and extended spawning periods

Ocean sunfish (Mola mola Linnaeus, 1758) monitoring program Spanish Trap fishery in the Western Mediterranean

Meeting of Subcommittee on Ecosystems and Bycatch, 8-12 May 2023, Madrid, SpainThe Subcommittee asked about the absence of tag recoveries and their causes and about the possibility of contributing biological samples from the Atlantic for the genetic analysis in the project. The presenter explained how the Mola mola are probably caught in traps during migration, which might explain the low number of recoveries. The low number of tagged fish to date could also contribute to the absence of recoveries. The presenter also welcomed the offer of analyzing samples from the Atlantic Ocean in the Mediterranean programme. The Subcommittee asked about the presence of Ranzania laevis in the catches of the Spanish traps, and it was clarified the project has not found Ranzania laevis in Spanish traps to dat