A phenomenological study examining how spirituality is manifested in the lives of student nurses whilst on clinical practice

This study aims to identify how spirituality is manifested in the lives of student nurses whilst on clinical practice. The area of spirituality and spiritual care is becoming an important issue within nursing practice and there has been a significant amount of research into understanding spirituality and examining the views of healthcare professionals. However there is limited research in the student nurse perspective, justifying this study. This was a piece of qualitative research involving unstructured interviews. The methodological approach taken was hermeneutic phenomenology which allowed for in depth responses to be provided. Ten 4th year Masters of Nursing Science students were interviews to gain a better insight into how they experience spiritual care in practice. The unstructured interviews were then recorded and transcribed. The analysis process found four key themes: Understanding of spiritual care and spirituality, Nurse Education and Practice, The role of the nurse in the provision of spiritual care and The impact of providing spiritual care on student nurses. The study found the views these participants had about spirituality and spiritual care were in many ways similar to those previously found of qualified nurses. It was apparent that there was a level of confusion over the meaning of spirituality and its role in nursing. However the students also recognised the importance of spirituality and the potential need for changes in the nurse curriculum to enable nurses to be more able to provide spiritual care. The voice of the student nurse is one that should be listened to. It seems that there is room and need for change in the nursing curriculum to ensure that student nurses have the knowledge and understanding of spirituality in future practice. The importance of reflective practice is apparent in this study and it should be utilised in spiritual care

Explorations of Classroom Talk and Links to Reading Achievement in Upper Elementary Classroo

The current study reports on a large-scale quantitative analysis of classroom talk practices and links to different measures of reading achievement within upper elementary classrooms. Data involving 745 fourth- and fifth-grade teachers and 18,844 students from the Measures of Effective Teaching (MET) study were used. Talk was quantified via various talk-related indicators from 2 observation protocols and a student survey. Dimensionality analyses suggest these indicators represent 4 factors consisting of teacher explaining, questioning, encouraging of student talk, and big-picture communicating. Links to 2 different standardized reading achievement measures were also modeled with improved ratings of teacher explanations and questioning predicting higher standardized reading scores. Relationships varied, though, by different measures of classroom talk (i.e., observational protocols vs. student surveys) and levels of analysis (i.e., the student, class period, or school level). Students’ but not observers’ ratings of talk practices linked to standardized reading at the class period level, whereas observers’ ratings related to standardized reading performance at the school level. Interpretations, implications for future research, and connections to educational practice are conveyed

Double blockade of CD14 and complement C5 abolishes the cytokine storm and improves morbidity and survival in polymicrobial sepsis in mice

Sepsis and septic shock, caused by an excessive systemic host-inflammatory response, are associated with high morbidity and mortality. The complement system and TLRs provide important pattern recognition receptors initiating the cytokine storm by extensive cross-talk. We hypothesized that double blockade of complement C5 and the TLR coreceptor CD14 could improve survival of experimental polymicrobial sepsis. Mice undergoing cecal ligation and puncture (CLP)–induced sepsis were treated with neutralizing anti-CD14 Ab biG 53, complement C5 inhibitor coversin (Ornithodoros moubata C inhibitor), or a combination thereof. The inflammatory study (24-h observation) revealed statistically significant increases in 22 of 24 measured plasma biomarkers in the untreated CLP group, comprising 14 pro- and anti-inflammatory cytokines and 8 chemokines, growth factors, and granulocyte activation markers. Single CD14 or C5 blockade significantly inhibited 20 and 19 of the 22 biomarkers, respectively. Combined CD14 and C5 inhibition significantly reduced all 22 biomarkers (mean reduction 85%; range 54–95%) compared with the untreated CLP group. Double blockade was more potent than single treatment and was required to significantly inhibit IL-6 and CXCL1. Combined inhibition significantly reduced morbidity (motility and eyelid movement) and mortality measured over 10 d. In the positive control CLP group, median survival was 36 h (range 24–48 h). Combined treatment increased median survival to 96 h (range 24–240 h) (p = 0.001), whereas survival in the single-treatment groups was not significantly increased (median and range for anti-CD14 and anti-C5 treatment were 36 h [24–48 h] and 48 h [24–96 h]). Combined with standard intervention therapy, specific blockade of CD14 and C5 might represent a promising new therapeutic strategy for treatment of polymicrobial sepsis

Genomics for public health and international surveillance of antimicrobial resistance.

Historically, epidemiological investigation and surveillance for bacterial antimicrobial resistance (AMR) has relied on low-resolution isolate-based phenotypic analyses undertaken at local and national reference laboratories. Genomic sequencing has the potential to provide a far more high-resolution picture of AMR evolution and transmission, and is already beginning to revolutionise how public health surveillance networks monitor and tackle bacterial AMR. However, the routine integration of genomics in surveillance pipelines still has considerable barriers to overcome. In 2022, a workshop series and online consultation brought together international experts in AMR and pathogen genomics to assess the status of genomic applications for AMR surveillance in a range of settings. Here we focus on discussions around the use of genomics for public health and international AMR surveillance, noting the potential advantages of, and barriers to, implementation, and proposing recommendations from the working group to help to drive the adoption of genomics in public health AMR surveillance. These recommendations include the need to build capacity for genome sequencing and analysis, harmonising and standardising surveillance systems, developing equitable data sharing and governance frameworks, and strengthening interactions and relationships among stakeholders at multiple levels

Intra-rater reliability, measurement precision, and inter-test correlations of 1RM single-leg leg-press, knee-flexion, and knee-extension in uninjured adult agility-sport athletes: Considerations for right and left unilateral measurements in knee injury control

Objectives Knowledge of single-leg knee strength test reliability for the right and left limb is critical for between-limb clinical decision-making. Knowledge of between-test correlations is essential for understanding whether tests measure similar or different aspects of muscle strength. This study investigated the intra-rater, test-retest reliability and measurement precision of one repetition maximum (1RM) single-leg leg-press (LP), knee-flexion (KF), and knee-extension (KE) for both limbs, and inter-test correlations. Design Repeated measures; Setting University. Participants Six males, seven females (age 25.6±5.5yr; height 171.4±8.4cm; mass 71.8±13.4kg). Main outcome measures Normalised 1RM (percent body-mass (%BM)), intraclass correlation coefficient (ICC) (Avery, 1995; Rivara, 2003), standard error of measurement (SEM; %BM), Pearson's correlation (r), coefficient of determination (r2). Results Mean 1RM test-retest values were (right, left): LP, 214.2–218.5%BM, 213.5–215.4%BM; KF, 35.9–38.9%BM, 37.7–38.2%BM; KE, 43.3–44.6%BM, 36.2–39.3%BM. The ICCs/SEMs were (right, left): LP, 0.98/7.3%BM, 0.94/14.2%BM; KF, 0.75/4.9%BM, 0.95/1.9%BM; KE, 0.87/3.4%BM, 0.78/4.4%BM. Correlations were significant (P < 0.01), r/r2 values were: LP-KF, 0.60/0.36; LP-KE, 0.59/0.35; KF-KE, 0.50/0.25. Conclusions Tests demonstrated good reliability and measurement precision, although ICCs and SEMs were different between limbs. Tests were correlated, but only one-third of the variance was shared between tests. Practitioners should be cognisant of between-limb differences in reliability and include all tests for knee clinical decision-making

Precise interpolar phasing of abrupt climate change during the last ice age

The last glacial period exhibited abrupt Dansgaard–Oeschger climatic oscillations, evidence of which is preserved in a variety of Northern Hemisphere palaeoclimate archives¹. Ice cores show that Antarctica cooled during the warm phases of the Greenland Dansgaard–Oeschger cycle and vice versa[superscript 2,3], suggesting an interhemispheric redistribution of heat through a mechanism called the bipolar seesaw[superscript 4–6]. Variations in the Atlantic meridional overturning circulation (AMOC) strength are thought to have been important, but much uncertainty remains regarding the dynamics and trigger of these abrupt events[superscript 7–9]. Key information is contained in the relative phasing of hemispheric climate variations, yet the large, poorly constrained difference between gas age and ice age and the relatively low resolution of methane records from Antarctic ice cores have so far precluded methane-based synchronization at the required sub-centennial precision[superscript 2,3,10]. Here we use a recently drilled high-accumulation Antarctic ice core to show that, on average, abrupt Greenland warming leads the corresponding Antarctic cooling onset by 218 ± 92 years (2σ) for Dansgaard–Oeschger events, including the Bølling event; Greenland cooling leads the corresponding onset of Antarctic warming by 208 ± 96 years. Our results demonstrate a north-to-south directionality of the abrupt climatic signal, which is propagated to the Southern Hemisphere high latitudes by oceanic rather than atmospheric processes. The similar interpolar phasing of warming and cooling transitions suggests that the transfer time of the climatic signal is independent of the AMOC background state. Our findings confirm a central role for ocean circulation in the bipolar seesaw and provide clear criteria for assessing hypotheses and model simulations of Dansgaard–Oeschger dynamics

A multimodal cell census and atlas of the mammalian primary motor cortex

ABSTRACT We report the generation of a multimodal cell census and atlas of the mammalian primary motor cortex (MOp or M1) as the initial product of the BRAIN Initiative Cell Census Network (BICCN). This was achieved by coordinated large-scale analyses of single-cell transcriptomes, chromatin accessibility, DNA methylomes, spatially resolved single-cell transcriptomes, morphological and electrophysiological properties, and cellular resolution input-output mapping, integrated through cross-modal computational analysis. Together, our results advance the collective knowledge and understanding of brain cell type organization: First, our study reveals a unified molecular genetic landscape of cortical cell types that congruently integrates their transcriptome, open chromatin and DNA methylation maps. Second, cross-species analysis achieves a unified taxonomy of transcriptomic types and their hierarchical organization that are conserved from mouse to marmoset and human. Third, cross-modal analysis provides compelling evidence for the epigenomic, transcriptomic, and gene regulatory basis of neuronal phenotypes such as their physiological and anatomical properties, demonstrating the biological validity and genomic underpinning of neuron types and subtypes. Fourth, in situ single-cell transcriptomics provides a spatially-resolved cell type atlas of the motor cortex. Fifth, integrated transcriptomic, epigenomic and anatomical analyses reveal the correspondence between neural circuits and transcriptomic cell types. We further present an extensive genetic toolset for targeting and fate mapping glutamatergic projection neuron types toward linking their developmental trajectory to their circuit function. Together, our results establish a unified and mechanistic framework of neuronal cell type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties

A phenomenological study examining how spirituality is manifested in the lives of student nurses whilst on clinical practice

This study aims to identify how spirituality is manifested in the lives of student nurses whilst on clinical practice. The area of spirituality and spiritual care is becoming an important issue within nursing practice and there has been a significant amount of research into understanding spirituality and examining the views of healthcare professionals. However there is limited research in the student nurse perspective, justifying this study. This was a piece of qualitative research involving unstructured interviews. The methodological approach taken was hermeneutic phenomenology which allowed for in depth responses to be provided. Ten 4th year Masters of Nursing Science students were interviews to gain a better insight into how they experience spiritual care in practice. The unstructured interviews were then recorded and transcribed. The analysis process found four key themes: Understanding of spiritual care and spirituality, Nurse Education and Practice, The role of the nurse in the provision of spiritual care and The impact of providing spiritual care on student nurses. The study found the views these participants had about spirituality and spiritual care were in many ways similar to those previously found of qualified nurses. It was apparent that there was a level of confusion over the meaning of spirituality and its role in nursing. However the students also recognised the importance of spirituality and the potential need for changes in the nurse curriculum to enable nurses to be more able to provide spiritual care. The voice of the student nurse is one that should be listened to. It seems that there is room and need for change in the nursing curriculum to ensure that student nurses have the knowledge and understanding of spirituality in future practice. The importance of reflective practice is apparent in this study and it should be utilised in spiritual care

Surface reactivation of FeNiPC metallic glass: A strategy for highly enhanced catalytic behavior

With intrinsically disordered atomic structure, metallic glasses (MGs) have been extensively studied as advanced catalysts. Although the nature of widely tunable atomic composition in MGs facilitates accommodation in different catalytic environments, it may also induce the decline of catalytic activity due to surface effect. The role of surface of MGs is still not fully understood. In this work, an Fe50Ni30P13C7 (FeNiPC) glassy ribbon was found to have a strong-binding surface layer with the superior anticorrosion but low catalytic ability. Nevertheless, an effective elimination of surface layer by chemical dealloying could highly promote the catalytic ability of FeNiPC glassy ribbons from 20 min to only 10 min for brilliant black BN dye degradation. Further investigation revealed that chemical dealloying could effectively dissolve the surface layer of FeNiPC glassy ribbons induced reactivation of surface that was previously buried under the strong oxide layer, instead of generating nano-porous structured surface. The reactivation of ribbon surface effectively optimized active reaction sites and the re-exposure of Fe, Ni and P with zero-valent state formed galvanic cells by atomic clusters leading to the acceleration of catalysis. The insight and revelation of intrinsic surface activity in this work provide an effective way to highly promote the catalytic ability of MGs