La Fitoterapia hoy: evidencias

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

La Fitoterapia hoy: evidencias

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

Actividad de albendazol y los aceites esenciales de menta (Mentha piperita) y manzanilla (Matricaria chamomilla) frente Anisakis tipo I

Objetivos: Estudiar la posible actividad sinérgica de los aceites esenciales de Mentha piperita y Matricaria chamomilla frente Anisakis tipo I. Material y métodos: Se ensayó tanto in vitro como in vivo, la actividad larvicida de la mezcla de los aceites esenciales utilizando albendazol como fármaco de referencia y midiéndo su eficacia mediante un modelo estadístico. Resultados: En los ensayos in vitro, a las concentraciones de 250 y 187.5 μg/ml, la mortalidad de las larvas fue del 100% mientras que albendazol no mostró eficacia. In vivo, la mezcla de aceites esenciales fue más eficaz, reduciendo las lesiones en ratas en comparación con las tratadas con albendazol y control. Albendazol no redujo de forma significativa el porcentaje de lesiones producidas por las larvas frente al control. Conclusión: La mezcla de aceite esencial de menta y manzanilla podría ser candidata para su uso en el tratamiento/profilaxis de la anisakiasis humana.Aim: We have studied the possible synergistic activity of the essential oils of Mentha piperita and Matricaria chamomile against Anisakis type I. Materials and methods: the larvicidal activity of the mixture of essential oils was tested in vitro and in vivo, using as a reference the drug Albendazole and effectiveness measured through a statistical model. Results: The in vitro and in vivo experiments both evidenced that the larvicidal activity of essential oils, was higher than the Albendazole activity. In the in vitro assay, at concentrations of 250 and 187.5 μg/ml, the mortality was 100% with the mixture of essential oils while albendazole was ineffective at the concentrations studied. In the in vivo assay, the mixture of essential oils, was significantly more effective in the reduction of numbers of lesions with rats in comparison to albendazole treatments and control. Albendazole did not significantly reduce the percentage of lesions caused by larvae vs. control. Conclusion: Considering the results, we have come to the conclusion that the mixture of peppermint essential oils and chamomile could be used in the treatment/prophylaxis of human Anisakis.Trabajo financiado por el proyecto: P07-CVI-03249

In vivo larvicidal activity of menthol against Anisakis type I

Se ha estudiado la actividad larvicida in vivo de mentol, principal componente del aceite esencial de Mentha pipperita, frente a larvas de Anisakis tipo I aisladas del hospedador Micromesistius poutassou (bacaladilla), adquiridas en diversas pescaderías de la ciudad de Granada. Los resultados obtenidos en el ensayo muestran que en el grupo tratado con mentol no se apreciaron lesiones en el aparato digestivo, mientras que en el grupo control estas lesiones fueron observadas en el 93’3% de los animales de experimentación. En este mismo grupo se encontraron larvas que habían perforado la pared gástrica, localizándose libres en la cavidad corporal.We have studied the in vivo larvicidal activity of menthol, the main component of the essential oil of Mentha pipperita, against Anisakis type I larvae isolated from the host Micromesistius poutassou (blue whiting), acquired in several fishmongers in the city of Granada. The in vivo test results showed no gastric wall lesions in the group treated with menthol, while in the control group, 93.3% of infected rats showed those lesions. In the same control group were found larvae that had drilled the gastric wall, located free in the abdominal cavity.Trabajo financiado por el proyecto: P07-CVI-03249

IN VIVO activity of essential oil Origanum elongatum against larva L3 of Anisakis pegreffii

En este trabajo se aborda la posible actividad larvicida in vivo del aceite esencial de Origanum elongatum, recolectado en Marruecos, frente a las larvas L3 de Anisakis pegreffii, que parasitan distintas especies marinas. Los resultados obtenidos son demostrativos del interés potencial de este aceite esencial como preventivo de la infección por Anisakis, tras ingestión del pescado parasitado.Anisakiasis is an emerging zoonotic disease caused by species of the genus Anisakis. In humans, this parasite is manifested by digestive symptoms. In the virtual absence of effective treatments against this infection, our working group has initiated a series of investigations aimed at finding natural products such as essential oils and their major components, which might be of interest in the treatment of the infectious form of these zoonoses. OBJECTIVE: Establishment of the possible in vivo activity of essential oil of O. elongatum, against L3 larva of Anisakis pegreffii. METHODOLOGY: For the study in vivo, parasites were isolated from the host Scomber japonicus (mackerel) and Trachurus trachurus (horse mackerel). The experimental animals (female Wistar rats) were infected with 6 Anisakis larva by gastric catheter, this technique was used also for the administration of O. elongatum (46.9 mg / 0.5 ml of olive oil), according to the following guidelines: infection and joint treatment and sacrifice at 4 hours. Parallel to this, a control test was performed, administering 0.5 ml olive oil together with six larvae of the parasite to a group of animals. The identification of the larvae was carried out using molecular techniques (PCR-RFLP). The identification of the main components of essential oil was performed by GC-MS.Trabajo financiado por el proyecto: P07-CVI-0324

Integrative epigenomics in Sjögren´s syndrome reveals novel pathways and a strong interaction between the HLA, autoantibodies and the interferon signature

Primary Sjögren's syndrome (SS) is a systemic autoimmune disease characterized by lymphocytic infiltration and damage of exocrine salivary and lacrimal glands. The etiology of SS is complex with environmental triggers and genetic factors involved. By conducting an integrated multi-omics study, we confirmed a vast coordinated hypomethylation and overexpression effects in IFN-related genes, what is known as the IFN signature. Stratified and conditional analyses suggest a strong interaction between SS-associated HLA genetic variation and the presence of Anti-Ro/SSA autoantibodies in driving the IFN epigenetic signature and determining SS. We report a novel epigenetic signature characterized by increased DNA methylation levels in a large number of genes enriched in pathways such as collagen metabolism and extracellular matrix organization. We identified potential new genetic variants associated with SS that might mediate their risk by altering DNA methylation or gene expression patterns, as well as disease-interacting genetic variants that exhibit regulatory function only in the SS population. Our study sheds new light on the interaction between genetics, autoantibody profiles, DNA methylation and gene expression in SS, and contributes to elucidate the genetic architecture of gene regulation in an autoimmune population

O31 Integrative analysis reveals a molecular stratification of systemic autoimmune diseases

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La Fitoterapia hoy: evidencias

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

Integrative epigenomics in Sjögren´s syndrome reveals novel pathways and a strong interaction between the HLA, autoantibodies and the interferon signature

International audienceAbstract Primary Sjögren’s syndrome (SS) is a systemic autoimmune disease characterized by lymphocytic infiltration and damage of exocrine salivary and lacrimal glands. The etiology of SS is complex with environmental triggers and genetic factors involved. By conducting an integrated multi-omics study, we confirmed a vast coordinated hypomethylation and overexpression effects in IFN-related genes, what is known as the IFN signature. Stratified and conditional analyses suggest a strong interaction between SS-associated HLA genetic variation and the presence of Anti-Ro/SSA autoantibodies in driving the IFN epigenetic signature and determining SS. We report a novel epigenetic signature characterized by increased DNA methylation levels in a large number of genes enriched in pathways such as collagen metabolism and extracellular matrix organization. We identified potential new genetic variants associated with SS that might mediate their risk by altering DNA methylation or gene expression patterns, as well as disease-interacting genetic variants that exhibit regulatory function only in the SS population. Our study sheds new light on the interaction between genetics, autoantibody profiles, DNA methylation and gene expression in SS, and contributes to elucidate the genetic architecture of gene regulation in an autoimmune population