En este trabajo se aborda la posible actividad larvicida in vivo del aceite esencial de Origanum
elongatum, recolectado en Marruecos, frente a las larvas L3 de Anisakis pegreffii, que parasitan
distintas especies marinas. Los resultados obtenidos son demostrativos del interés potencial de este
aceite esencial como preventivo de la infección por Anisakis, tras ingestión del pescado parasitado.Anisakiasis is an emerging zoonotic disease caused by species of the genus Anisakis. In humans, this
parasite is manifested by digestive symptoms. In the virtual absence of effective treatments against
this infection, our working group has initiated a series of investigations aimed at finding natural
products such as essential oils and their major components, which might be of interest in the treatment
of the infectious form of these zoonoses. OBJECTIVE: Establishment of the possible in vivo activity
of essential oil of O. elongatum, against L3 larva of Anisakis pegreffii.
METHODOLOGY: For the study in vivo, parasites were isolated from the host Scomber japonicus
(mackerel) and Trachurus trachurus (horse mackerel). The experimental animals (female Wistar rats)
were infected with 6 Anisakis larva by gastric catheter, this technique was used also for the
administration of O. elongatum (46.9 mg / 0.5 ml of olive oil), according to the following guidelines:
infection and joint treatment and sacrifice at 4 hours. Parallel to this, a control test was performed,
administering 0.5 ml olive oil together with six larvae of the parasite to a group of animals. The
identification of the larvae was carried out using molecular techniques (PCR-RFLP). The
identification of the main components of essential oil was performed by GC-MS.Trabajo financiado por el proyecto: P07-CVI-0324
