45 research outputs found
Expression of genes for estrogen receptors Ī± and Ī² in human articular chondrocytes
AbstractObjective To investigate the gene expression of estrogen receptor (ER) Ī± and ERĪ² in human articular chondrocytes.Methods 16 articular cartilage specimens were obtained from 15 patients during surgery. Three of the specimens were from men and 13 from women; three from hip joints and 13 from knee joints; four were normal and 12 showed osteoarthritic cartilage. Total RNA was extracted from the articular chondrocytes and the expression of both ERĪ± and ERĪ² genes was investigated by the reverse transcription-polymerase chain reaction (RT-PCR) method.Results Gene expressions of ERĪ± were detected in all specimens and those of ERĪ² were found in 15 specimens by the RT-PCR method. There was a significant correlation between the amounts of ERĪ± and ERĪ². Expression levels of both genes were significantly higher in men than in women. There were no significant differences in the expression levels of both ER genes between the hip and knee joint sites, nor between normal and osteoarthritic tissues.Conclusion This study is to our knowledge the first to demonstrate the gene expression of both ERĪ± and ERĪ² in human articular chondrocytes. Since there are some functional differences between the two receptors, the effects of estrogen on cartilage metabolism should be elucidated by two different receptor mechanisms.{copy
Sex-specific response of rat costochondral cartilage growth plate chondrocytes to 17Ī²-estradiol involves differential regulation of plasma membrane associated estrogen receptors
Sexual dimorphism of growth plate prehypertrophic and hypertrophic chondrocytes in response to testosterone requires metabolism to dihydrotestosterone (DHT) by steroid 5-alpha reductase type 1
The effect of estrogen on the expression of cartilage-specific genes in the chondrogenesis process of adipose-derived stem cells
Porcine Fetal Enamel Matrix Derivative Stimulates Proliferation But Not Differentiation of Pre-Osteoblastic 2T9 Cells, Inhibits Proliferation and Stimulates Differentiation of Osteoblast-Like MG63 Cells, and Increases Proliferation and Differentiation of Normal Human Osteoblast NHOst Cells
Evidence for Receptors Specific for 17Ī²-Estradiol and Testosterone in Chondrocyte Cultures
Biocompatibility of four common orthopedic biomaterials following neuroelectromyostimulation: An ināvivo
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