Amino functionalized mesoporous silica nanoparticles encapsulated octahedral organoruthenium complex as an efficient platform for combatting cáncer.

In the process of synthesis of a new drug, as important as the drug itself is the formulation used, because the same compound can present a very different efficacy depending on how it is administered. In this work, we demonstrate how the antitumor capacity of a new octahedral organo-ruthenium complex, [Ru(ppy-CHO)(phen)2][PF6] is affected by its encapsulation in different types of mesoporous silica nanoparticles. The interactions between the Ru complex and the silica matrix and how these interactions are affected at two different pHs (7.4 and 5.4, mimicking physiological and endolysosomal acidic conditions, respectively) have been studied. The encapsulation has also been shown to affect the induction of apoptosis and necrosis and progression of the cell cycle compared to the free drug. The encapsulation of the Ru complex in nanoparticles functionalized with amino groups produced very high anticancer activity in cancer cells in vitro, especially against U87 glioblastoma cells, favoring cellular internalization and significantly increasing the anticancer capacity of the initial non-encapsulated Ru complex

Autoantibodies against type I IFNs in patients with critical influenza pneumonia

In an international cohort of 279 patients with hypoxemic influenza pneumonia, we identified 13 patients (4.6%) with autoantibodies neutralizing IFN-alpha and/or -omega, which were previously reported to underlie 15% cases of life-threatening COVID-19 pneumonia and one third of severe adverse reactions to live-attenuated yellow fever vaccine. Autoantibodies neutralizing type I interferons (IFNs) can underlie critical COVID-19 pneumonia and yellow fever vaccine disease. We report here on 13 patients harboring autoantibodies neutralizing IFN-alpha 2 alone (five patients) or with IFN-omega (eight patients) from a cohort of 279 patients (4.7%) aged 6-73 yr with critical influenza pneumonia. Nine and four patients had antibodies neutralizing high and low concentrations, respectively, of IFN-alpha 2, and six and two patients had antibodies neutralizing high and low concentrations, respectively, of IFN-omega. The patients' autoantibodies increased influenza A virus replication in both A549 cells and reconstituted human airway epithelia. The prevalence of these antibodies was significantly higher than that in the general population for patients 70 yr of age (3.1 vs. 4.4%, P = 0.68). The risk of critical influenza was highest in patients with antibodies neutralizing high concentrations of both IFN-alpha 2 and IFN-omega (OR = 11.7, P = 1.3 x 10(-5)), especially those <70 yr old (OR = 139.9, P = 3.1 x 10(-10)). We also identified 10 patients in additional influenza patient cohorts. Autoantibodies neutralizing type I IFNs account for similar to 5% of cases of life-threatening influenza pneumonia in patients <70 yr old

Ecosystem change and zoonoses in the Anthropocene

International audienc

Room temperature operation of Er-doped silicon-rich oxide microcavities supporting high-Q whispering-gallery modes.

In the past few years, many studies have been carried out to use the ability of light to transport information into silicon-based integrated photonic circuits. The realization of an efficient silicon-based light source is therefore necessary but however challenging. Lasing cannot be easily achieved from silicon emission because of its indirect bandgap. Therefore, one solution proposed is to use other efficient emitters, like rare earth, into silicon or Silicon On Insulator based microcavities. Silica microdisk has been demonstrated to support high-Q whispering-gallery modes, and can be upgraded to ultra-high-Q toroidal microcavities by a CO2 laser melting process. Microdisk high Q-factor balances the low gain generally obtained from the active medium. Thus, those microcavities may be good candidatesfor silicon-based laser. In this paper, the fabrication and roomtemperature operation of silica microdisk associated with Er-doped silicon rich oxide is presented. Er atoms are excited at the 351 nm wavelength via the silicon clusters, giving to the material a high photonic capture section, and therefore a good photoluminescence efficiency. We demonstrate efficient coupling of erbium atoms to high-Q whispering-gallery modes. The photoluminescence spectrum is then theoretically treated. The WGM resonances are thus identified. We also discuss the contribution of the spot excitation and the weak coupling to the higher radial order modes. Finally, the polarization dependence of the observed modes is investigated, and the experimental results are compared to our analytical model of disk-shape cavities. Those results give us to think that an integrated laser should be soon achieved

Nitrogen dioxide and allergic sensitization in the 2005–2006 National Health and Nutrition Examination Survey

SummaryBackgroundAllergic sensitization is a risk factor for asthma and allergic diseases. The relationship between ambient air pollution and allergic sensitization is unclear.ObjectiveTo investigate the relationship between ambient air pollution and allergic sensitization in a nationally representative sample of the US population.MethodsWe linked annual average concentrations of nitrogen dioxide (NO2), particulate matter ≤10 μm (PM10), particulate matter ≤2.5 μm (PM2.5), and summer concentrations of ozone (O3), to allergen-specific immunoglobulin E (IgE) data for participants in the 2005–2006 National Health and Nutrition Examination Survey (NHANES). In addition to the monitor-based air pollution estimates, we used the Community Multiscale Air Quality (CMAQ) model to increase the representation of rural participants in our sample. Logistic regression with population-based sampling weights was used to calculate adjusted prevalence odds ratios per 10 ppb increase in O3 and NO2, per 10 μg/m3 increase in PM10, and per 5 μg/m3 increase in PM2.5 adjusting for race, gender, age, socioeconomic status, smoking, and urban/rural status.ResultsUsing CMAQ data, increased levels of NO2 were associated with positive IgE to any (OR 1.15, 95% CI 1.04, 1.27), inhalant (OR 1.17, 95% CI 1.02, 1.33), and indoor (OR 1.16, 95% CI 1.03, 1.31) allergens. Higher PM2.5 levels were associated with positivity to indoor allergen-specific IgE (OR 1.24, 95% CI 1.13, 1.36). Effect estimates were similar using monitored data.ConclusionsIncreased ambient NO2 was consistently associated with increased prevalence of allergic sensitization

Flavonoid-rich fruit and vegetables improve microvascular reactivity and inflammatory status in men at risk of cardiovascular disease-FLAVURS:a randomized controlled trial

Observed associations between increased fruit and vegetable (F&V) consumption, particularly those F&Vs that are rich in flavonoids, and vascular health improvements require confirmation in adequately powered randomized controlled trials