43 research outputs found

    Estudio comparativo de las condiciones de vida de las familias desplazadas por la violencia en Urabá

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    Por convenio entre la Universidad de Antioquia- Instituto de Estudios Regionales (INER) y la Cruz Roja programa Urabá - Cruz Roja Sueca, se está realizando este estudio con miras a conocer las condiciones de vida de las familias desplazadas por la violencia en Urabá e, igualmente, crear conciencia en las familias y en la población para controlar la violencia a nivel micro y macro; buscar para las familias desplazadas, en caso de retorno, garantías a la conservación de sus tierras y a la seguridad e integridad; contribuir a frenar el desplazamiento, y difundir nacional e internacionalmente los resultados obtenidos con el fin de llamar la atención sobre un fenómeno que afecta directamente a la familia y encadena a poblaciones entera

    High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey

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    [EN] Quantification of chemical residues in honey is a market requirement to ensure consumer safety. The most common method used to analyze these compounds is the LC/MS/MS methodology, which requires highly qualified technicians and a tedious pre-treatment of the sample. The honey-packaging industry needs cheaper and faster alternatives for routine control. HFF-QCM (High Fundamental Frequency Quartz Crystal Microbalance) sensors are becoming a good option due to their high sensitivity, fast detection and low cost, while avoiding complex sample pre-treatment. The HFF-QCM technology is based on piezoelectric sensors with frequencies in the range from several tenths of MHz to hundreds of MHz. In this work a 100 MHz HFF-QCM sensor was used in a monoclonal antibody-based competitive immunoassay for specific bio-recognition of carbaryl pesticide as testing contaminant. The work intends to validate the use of HFF-QCM technology, in comparison with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique, for the detection of contaminants in honey. For this purpose, the validation criteria required by SANCO 12571/2013 guidance document were considered. The precision and accuracy (recovery) of both methods were determined by comparison of 5 replicates at 4 different concentrations (from 0 to 100 ¿g/kg) using the same honey matrix. HFF-QCM technology showed good accuracy, with recovery percentages always between 110 and 120%. As regards to precision, HFF-QCM coefficients of variation (CV) were around 10% higher than those recommended by GC SANCO 12571/2013. HFF-QCM limits of detection (LOD) and quantification (LOQ) were in the same order of magnitude as those for LC-MS/MS, which allows the analysis of carbaryl residues in honey under the established maximum residue limits (MRL), without sample pre-treatment. These results show that biosensors based on HFF-QCM technology has become a serious alternative to the traditional analytical techniques for food quality and safety applications.The authors are grateful for financial support from the Ministry of Economy and Competitiveness of Spain under the project AGL2013-48646-R. We also want to thank “Generalitat Valenciana” for the financial support to hire research personnel through the grant “Ayudas para la contratación de personal investigador en formación de carácter predoctoral” to carry out this research (DOC Num.7615/15.09.2015).Cervera-Chiner, L.; Juan Borrás, MDS.; March, C.; Arnau Vives, A.; Escriche Roberto, MI.; Montoya, Á.; Jiménez Jiménez, Y. (2018). High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey. Food Control. 92:1-6. https://doi.org/10.1016/j.foodcont.2018.04.026S169

    High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey

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    [EN] In this study, a piezoelectric immunosensor based on High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) technology was developed for detection of sulfathiazole in honey. The biorecognition was based on a competitive immunoassay in the conjugate-coated format, using monoclonal antibodies as specific im-munoreagents. The quantification of sulfathiazole was performed by building the corresponding calibration standard curve in diluted honey (1/140). Due to the competitive nature of the immunoassay, the standard curve showed a sigmoidal pattern with limits of detection (LOD) and quantification (LOQ) of 0.10 ¿g/kg and 2 ¿g/kg honey, respectively. The LOD reached by this immunosensor is 40¿50 times lower than those reported by other techniques for antibiotic detection. Moreover, this method requires minimum honey pre-treatment, making it faster and simpler than other methods. This immunosensor meets the precision and accuracy requirements established by SANCO guidelines, when sulfathiazole concentration in honey is not lower than 10 ¿g/kg. These findings could be the basis for reaching enough reliability for lower concentrations. Therefore, HFF-QCMD immunosensors can be considered a feasible alternative to current techniques for rapid and highly sensitive determination of sulfathiazole in honey with minimum sample preparation.The Spanish Ministry of Economy and Competitiveness, with FEDER-funds, has financially supported this work through the AGL 2013-48646-R project. Furthermore, authors are grateful for financial support from the European Social Fund and the Generalitat Valenciana for the PhD grant (Ayudas para la contratación de personal investigador en formación de carácter predoctoral) to Lourdes Cervera Chiner (ACIF/2016/132). The authors would also like to thank the Universitat Politècnica de València for the support provided for contracting Marisol Juan Borrás in the frame of PAID-10-17 (Ayuda para la contratación de acceso al Sistema Español de Ciencia, Tecnología e Innovación en Estructuras de Investigación).Cervera-Chiner, L.; Jiménez Jiménez, Y.; Montoya, Á.; Juan-Borras, MDS.; Pascual, N.; Arnau Vives, A.; Escriche Roberto, MI. (2020). High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey. Food Control. 115:1-6. https://doi.org/10.1016/j.foodcont.2020.107296S1611

    Meaning of the academic practices implemented in PRISA

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    ABSTRACT: It was systematization method applied to the project for a healthy childhood-PRISA-academic practices that involved six health programs from the University of Antioquia. The systematization begun with a reflexive process involving a systematic and continue accurate methodological processes that allowed us to find the meaning of actor’s experiences working in PRISA and participating in the systematization method. The process led us to establish the objectives of the systematization and the definition of investigative techniques more appropriate for the actors of PRISA in order to find the meaning of their experiences. The following objectives were proposed: a) to investigate the concept of academic practice and social academic units that are applied by people working in PRISA. b) To investigate actor’s academic practice outcomes working in PRISA. We found with the systematization method that PRISA was a place for learning experiences. The concept of instrumental and instructive practice was essential, for teachers, students and community who found meaning for this practice, this experience made they to take pedagogical and social actions, to search for other meanings, creating new purposes and motivations that may go beyond the institutional proposals, establishing new relationships and therefore generating knowledge for themselves.RESUMEN: La presente sistematización se realizó en el marco del Proyecto Interinstitucional por una Infancia Saludable (PRISA), proyecto interinstitucional de prácticas académicas en el que participaron seis facultades del área de la salud de la Universidad de Antioquia. La sistematización partió de un proceso reflexivo y continúo con un riguroso transcurso metodológico a través del cual se encontró el sentido a las voces de los actores participantes en PRISA y en la sistematización. El proceso de reflexión e indagación conllevó al establecimiento de los objetivos de la sistematización y a la definición de las técnicas de investigación más propicias para interactuar con los actores de PRISA y encontrar el sentido a sus vivencias. Se establecieron los siguientes objetivos; entre otros: a) indagar por el concepto de Práctica Social y académica de las unidades académicas que participaron en PRISA.b) indagar por el sentido de las prácticas académicas para los actores participantes en PRISA. Entre los hallazgos de la sistematización se evidencia que PRISA constituyó un escenario de vivencias y de aprendizajes, de esta manera el concepto instrumental e instructivo de la práctica se transformó, porque profesores, estudiantes y comunidad le dieron otros sentidos a la misma, generando acciones pedagógicas y sociales que, en la búsqueda de otros significados, configuraron nuevos propósitos y commotivaciones que trascendieron lo propuesto institucionalmente; instaurándose nuevas relaciones e intersubjetividades y, por lo tanto, generando aprendizajes desde la práctica

    Hydrophobic, hydrophilic and charged amino acids' networks within Protein

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    The native three dimensional structure of a single protein is determined by the physico chemical nature of its constituent amino acids. The twenty different types of amino acids, depending on their physico chemical properties, can be grouped into three major classes - hydrophobic, hydrophilic and charged. We have studied the anatomy of the weighted and unweighted networks of hydrophobic, hydrophilic and charged residues separately for a large number of proteins. Our results show that the average degree of the hydrophobic networks has significantly larger value than that of hydrophilic and charged networks. The average degree of the hydrophilic networks is slightly higher than that of charged networks. The average strength of the nodes of hydrophobic networks is nearly equal to that of the charged network; whereas that of hydrophilic networks has smaller value than that of hydrophobic and charged networks. The average strength for each of the three types of networks varies with its degree. The average strength of a node in charged networks increases more sharply than that of the hydrophobic and hydrophilic networks. Each of the three types of networks exhibits the 'small-world' property. Our results further indicate that the all amino acids' networks and hydrophobic networks are of assortative type. While maximum of the hydrophilic and charged networks are of assortative type, few others have the characteristics of disassortative mixing of the nodes. We have further observed that all amino acids' networks and hydrophobic networks bear the signature of hierarchy; whereas the hydrophilic and charged networks do not have any hierarchical signature.Comment: Corresponding author: Sudip Kund

    Evolving Clustered Random Networks

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    We propose a Markov chain simulation method to generate simple connected random graphs with a specified degree sequence and level of clustering. The networks generated by our algorithm are random in all other respects and can thus serve as generic models for studying the impacts of degree distributions and clustering on dynamical processes as well as null models for detecting other structural properties in empirical networks

    The use of interactive response systems as a tool to favor proactive learning in Engineering

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    El desarrollo de las tecnologías de la información y la comunicación (TIC) ha permitido el surgimiento de herramientas didácticas en el campo de la educación, proporcionando herramientas prácticas para apoyar a las clases presenciales. En este contexto, los sistemas electrónicos de respuesta estudiantil pueden ser útiles para introducir un elemento tecnológico motivador en las lecciones, así como una nueva metodología. En este estudio, además del uso de los sistemas de respuesta interactiva o clickers, se ha introducido la tecnología de aprendizaje móvil mediante la elaboración de una metodología de uso de las herramientas Kahoot y Telegram en la asignatura "Fundamentos Físicos en la Ingeniería II" del Grado en Ingeniería Electrónica Industrial. El Departamento de Física Aplicada de la Universidad de Córdoba tiene una amplia experiencia en el uso de Clickers en clases teóricas con grupos grandes para diferentes grados universitarios, pero ahora el uso de tecnologías móviles de aprendizaje se ha introducido en grupos de tamaño medio para clases prácticas. Usando esta nueva metodología, los estudiantes de grupos medianos realizan un cuestionario durante la lección de resolución de problemas, donde utilizaron sus conocimientos adquiridos durante la clase. La realización del cuestionario permite a los profesores evaluar en tiempo real el nivel del estudiante y utilizar la retroalimentación para abordar los problemas iniciales y los malentendidos. Los resultados muestran que los sistemas de respuesta interactiva son altamente valorados por los estudiantes, que lo perciben como una herramienta para mejorar el aprendizaje y aumentar la competencia en el aula.The development of information and communication technologies (ICT) has enabled the emergence of teaching tools in the education field, providing practical tools to support face-to-face classes. In this context, electronic student response systems can be useful for introducing a motivating technological element into the lessons, as well as a new methodology. In this study, in addition to the use of interactive response systems or clickers, mobile learning technology has been introduced by developing a methodology for using the Kahoot and Telegram tools in the subject “Physical Foundations of Engineering II” of Electronic Engineering Degree. Department of Applied Physics of University of Cordoba has a broad experience using Clickers in theory classes with large groups for different university degrees, but now the use of mobile learning technologies has been introduced in medium-sized groups for practical classes. Using this new methodology, students of medium-sized groups perform a quiz during the problem solving lesson, where they utilized their knowledge gained during the class. The completion of the quiz allows teachers to assess the student’s level in real time, and to use the feedback to address initial problems and misunderstandings. Results show the interactive response systems are highly valued by students, who perceive it as a tool to improve learning and increase competition in the classroom

    Experiencias docentes en tiempo de pandemia

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    El texto Experiencias docentes en tiempo de pandemia está estructurado en dos partes: 1. Discusiones necesarias sobre la educación superior en tiempo de pandemia. 2. Prácticas docentes en época de pandemia expresadas a través del relato. En la primera parte, se presenta un conjunto de nueve artículos que buscan fundamentar teóricamente la situación actual de la educación conforme a las circunstancias que estamos viviendo, de manera que: Robert Fernando Bolaños Vivas, en su artículo La filosofía de la Educación ante la crisis sanitaria COVID-19, una oportunidad de humanización, considera que la crisis sanitaria de la COVID-19 ha puesto de manifesto la profunda crisis humana que pone a prueba la calidad de los seres humanos, razón por la cual el autor intenta demostrar la deficiente respuesta antropológica a las complejas exigencias y complejidades de la crisis sanitaria provocada por la COVID- 19, en este sentido, en el artículo reflexiona sobre la dimensión de la alteridad, la historicidad y la temporalidad humana

    Gestión del conocimiento: perspectiva multidisciplinaria. Volumen 12

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    El libro “Gestión del Conocimiento. Perspectiva Multidisciplinaria”, Volumen 12, de la Colección Unión Global, es resultado de investigaciones. Los capítulos del libro, son resultados de investigaciones desarrolladas por sus autores. El libro cuenta con el apoyo de los grupos de investigación: Universidad Sur del Lago “Jesús María Semprúm” (UNESUR), Zulia – Venezuela; Universidad Politécnica Territorial de Falcón Alonso Gamero (UPTAG), Falcón – Venezuela; Universidad Politécnica Territorial de Mérida Kleber Ramírez (UPTM), Mérida – Venezuela; Universidad Guanajuato (UG) - Campus Celaya - Salvatierra - Cuerpo Académico de Biodesarrollo y Bioeconomía en las Organizaciones y Políticas Públicas (C.A.B.B.O.P.P), Guanajuato – México; Centro de Altos Estudios de Venezuela (CEALEVE), Zulia – Venezuela, Centro Integral de Formación Educativa Especializada del Sur (CIFE - SUR) - Zulia - Venezuela, Centro de Investigaciones Internacionales SAS (CIN), Antioquia - Colombia.y diferentes grupos de investigación del ámbito nacional e internacional que hoy se unen para estrechar vínculos investigativos, para que sus aportes científicos formen parte de los libros que se publiquen en formatos digital e impreso

    “La historia es así: o ellos o nosotros”

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