Estudio clínico de un brote de peritonitis infecciosa felina

Se describe un brote de peritonitis infecciosa felina (P.L.F) en una comunidad de 6 gatos. La baja prevalencia de la enfermedad, la inespecificidad de sus síntomas y la complejidad del diagnóstico dieron como resultado que sólo se detectase la enfermedad en e! estudio ana tomo patológico post-mortern del primer caso. Posteriormente, este diagnóstico se confirmó mediante necropsia y análisishistopatológico de otro de los gatos que contrajeron la enfermedad. No se ha encontrado ninguna referencia anterior de esta enfermedad en España, por lo que creemos que se trata de la primera descripción de P.L.F en nuestro país.An outbreak offeline infectious peritonitis (F.l.P.) is described in a community of six cats. The low prevalence of the disease, the non-specific nature of its symptoms and the complexity of diagnosis gave, as their result, that it could only be detected in the post-mortem anatornical-pathological study of the first case. Later on, this diagnosis was supported by means of the necropsy and histopathological analysis of one of the other cats that caught the disease. No previous reference to this disease has been found in Spain, due to which we belieue this may be considered as thefirst description of Feline Infectious Peritonitis in our country

Immunometabolism modulation in therapy

The study of cancer biology should be based around a comprehensive vision of the entire tumor ecosystem, considering the functional, bioenergetic and metabolic state of tumor cells and those of their microenvironment, and placing particular importance on immune system cells. Enhanced understanding of the molecular bases that give rise to alterations of pathways related to tumor development can open up new therapeutic intervention opportunities, such as metabolic regulation applied to immunotherapy. This review outlines the role of various oncometabolites and immunometabolites, such as TCA intermediates, in shaping pro/anti-inflammatory activity of immune cells such as MDSCs, T lymphocytes, TAMs and DCs in cancer. We also discuss the extraordinary plasticity of the immune response and its implication in immunotherapy efficacy, and highlight different therapeutic intervention possibilities based on controlling the balanced systems of specific metabolites with antagonistic functions

Estimación de la huella hídrica de baldosas de gres porcelánico esmaltado

Resumen de la Presentació oral presentada al LIII Congreso de la Sociedad Española de Cerámica y Vidrio (SECV) y XII Congreso del Técnico Cerámico (ATC), celebrat a l'Escola Española de Cerámica y Vidrio, Alcora (Castellón) del 23 al 25 d'octubre de 2013.En este trabajo se presenta un primer estudio de la estimación de la huella hídrica de las baldosas de gres porcelánico esmaltado, realizado a partir de datos sectoriales, es decir, no es un estudio específico de ninguna empresa en particular y con el soporte de bases de datos de ACV (concretamente PE International, ILCD)

On the coherence loss in phase-referenced VLBI observations

Context: Phase referencing is a standard calibration technique in radio interferometry, particularly suited for the detection of weak sources close to the sensitivity limits of the interferometers. However, effects from a changing atmosphere and inaccuracies in the correlator model may affect the phase-referenced images, leading to wrong estimates of source flux densities and positions. A systematic observational study of signal decoherence in phase referencing, and its effects in the image plane, has not been performed yet. Aims: We systematically studied how the signal coherence in Very-Long-Baseline-Interferometry (VLBI) observations is affected by a phase-reference calibration at different frequencies and for different calibrator-to-target separations. The results obtained should be of interest for a correct interpretation of many phase-referenced observations with VLBI. Methods: We observed a set of 13 strong sources (the S5 polar cap sample) at 8.4 and 15 GHz in phase-reference mode, with 32 different calibrator/target combinations spanning angular separations between 1.5 and 20.5 degrees. We obtained phase-referenced images and studied how the dynamic range and peak flux density depend on observing frequency and source separation. Results: We obtained dynamic ranges and peak flux densities of the phase-referenced images as a function of frequency and separation from the calibrator. We compared our results with models and phenomenological equations previously reported. Conclusions: The dynamic range of the phase-referenced images is strongly limited by the atmosphere at all frequencies and for all source separations. The limiting dynamic range is inversely proportional to the sine of the calibrator-to-target separation. We also find that the peak flux densities, relative to those obtained with the self-calibrated images, decrease with source separation.Comment: 5 pages, 3 figures. Submitted to A&A on 5.02.2010; accepted on 11.03.2010

Role of small Rhizaria and diatoms in the pelagic silica production of the Sourther Ocean

We examined biogenic silica production and elementary composition (biogenic Si, particulate organic carbon and particulate organic nitrogen) of Rhizaria and diatoms in the upper 200 m along a transect in the Southwest Pacific sector of the Southern Ocean during austral summer (January–February 2019). From incubations using the 32Si radioisotope, silicic acid uptake rates were measured at 15 stations distributed in the Polar Front Zone, the Southern Antarctic Circumpolar Current and the Ross Sea Gyre. Rhizaria cells are heavily silicified (up to 7.6 nmol Si cell−1), displaying higher biogenic Si content than similar size specimens found in other areas of the global ocean, suggesting a higher degree of silicification of these organisms in the silicic acid rich Southern Ocean. Despite their high biogenic Si and carbon content, the Si/C molar ratio (average of 0.05 ± 0.03) is quite low compared to that of diatoms and relatively constant regardless of the environmental conditions. The direct measurements of Rhizaria's biogenic Si production (0.8–36.8 μmol Si m−2 d−1) are of the same order of magnitude than previous indirect estimations, confirming the importance of the Southern Ocean for the global Rhizaria silica production. However, diatoms largely dominated the biogenic Si standing stock and production of the euphotic layer, with low rhizarians' abundances and biogenic Si production (no more than 1%). In this manuscript, we discuss the Antarctic paradox of Rhizaria, that is, the potential high accumulation rates of biogenic Si due to Rhizaria in siliceous sediments despite their low production rates in surface waters.Versión del editor3,38

Genetic and Chemical Modifiers of a CUG Toxicity Model in Drosophila

Non-coding CUG repeat expansions interfere with the activity of human Muscleblind-like (MBNL) proteins contributing to myotonic dystrophy 1 (DM1). To understand this toxic RNA gain-of-function mechanism we developed a Drosophila model expressing 60 pure and 480 interrupted CUG repeats in the context of a non-translatable RNA. These flies reproduced aspects of the DM1 pathology, most notably nuclear accumulation of CUG transcripts, muscle degeneration, splicing misregulation, and diminished Muscleblind function in vivo. Reduced Muscleblind activity was evident from the sensitivity of CUG-induced phenotypes to a decrease in muscleblind genetic dosage and rescue by MBNL1 expression, and further supported by the co-localization of Muscleblind and CUG repeat RNA in ribonuclear foci. Targeted expression of CUG repeats to the developing eye and brain mushroom bodies was toxic leading to rough eyes and semilethality, respectively. These phenotypes were utilized to identify genetic and chemical modifiers of the CUG-induced toxicity. 15 genetic modifiers of the rough eye phenotype were isolated. These genes identify putative cellular processes unknown to be altered by CUG repeat RNA, and they include mRNA export factor Aly, apoptosis inhibitor Thread, chromatin remodelling factor Nurf-38, and extracellular matrix structural component Viking. Ten chemical compounds suppressed the semilethal phenotype. These compounds significantly improved viability of CUG expressing flies and included non-steroidal anti-inflammatory agents (ketoprofen), muscarinic, cholinergic and histamine receptor inhibitors (orphenadrine), and drugs that can affect sodium and calcium metabolism such as clenbuterol and spironolactone. These findings provide new insights into the DM1 phenotype, and suggest novel candidates for DM1 treatments

The challenge and scientific application of the CO2 4.3 um atmospheric limb emission of Mars

The atmospheric fluorescent emissions of CO2 at 4.3- um have been observed in the daytime upper atmosphere of Mars from a limb geometry by the instruments OMEGA and PFS on board Mars Express [1, 8]. Initial analysis using non-local thermodynamic equilibrium (NLTE) models show that the emissions are well understood [7, 3, 6]. Yet they have not been exploited to derive important thermospheric parameters, like CO2 densities and temperatures. Our major goals are to improve current NLTE models with a joint study of OMEGA and PFS data, and to build an ambitious state-of-the-art NLTE retreival scheme for Mars. Recent progress has been made in these directions on Mars, Venus and Earth. We will present a summary of these efforts and the difficulties and expectatives for its application to the Mars Express dat

Drosophila muscleblind Codes for Proteins with One and Two Tandem Zinc Finger Motifs

Muscleblind-like proteins, Muscleblind (Mbl) in Drosophila and MBNL1-3 in vertebrates, are regulators of alternative splicing. Human MBNL1 is a key factor in the etiology of myotonic dystrophy (DM), a muscle wasting disease caused by the occurrence of toxic RNA molecules containing CUG/CCUG repeats. MBNL1 binds to these RNAs and is sequestered in nuclear foci preventing it from exerting its normal function, which ultimately leads to mis-spliced mRNAs, a major cause of the disease. Muscleblind-proteins bind to RNAs via N-terminal zinc fingers of the Cys3-His type. These zinc fingers are arranged in one (invertebrates) or two (vertebrates) tandem zinc finger (TZF) motifs with both fingers targeting GC steps in the RNA molecule. Here I show that mbl genes in Drosophila and in other insects also encode proteins with two TZF motifs, highly similar to vertebrate MBNL proteins. In Drosophila the different protein isoforms have overlapping but possibly divergent functions in vivo, evident by their unequal capacities to rescue the splicing defects observed in mbl mutant embryos. In addition, using whole transcriptome analysis, I identified several new splicing targets for Mbl in Drosophila embryos. Two of these novel targets, kkv (krotzkopf-verkehrt, coding for Chitin Synthase 1) and cora (coracle, coding for the Drosophila homolog of Protein 4.1), are not muscle-specific but expressed mainly in epidermal cells, indicating a function for mbl not only in muscles and the nervous system

Investigations of the Mars Upper Atmosphere with ExoMars Trace Gas Orbiter

The Martian mesosphere and thermosphere, the region above about 60 km, is not the primary target of the ExoMars 2016 mission but its Trace Gas Orbiter (TGO) can explore it and address many interesting issues, either in-situ during the aerobraking period or remotely during the regular mission. In the aerobraking phase TGO peeks into thermospheric densities and temperatures, in a broad range of latitudes and during a long continuous period. TGO carries two instruments designed for the detection of trace species, NOMAD and ACS, which will use the solar occultation technique. Their regular sounding at the terminator up to very high altitudes in many different molecular bands will represent the first time that an extensive and precise dataset of densities and hopefully temperatures are obtained at those altitudes and local times on Mars. But there are additional capabilities in TGO for studying the upper atmosphere of Mars, and we review them briefly. Our simulations suggest that airglow emissions from the UV to the IR might be observed outside the terminator. If eventually confirmed from orbit, they would supply new information about atmospheric dynamics and variability. However, their optimal exploitation requires a special spacecraft pointing, currently not considered in the regular operations but feasible in our opinion. We discuss the synergy between the TGO instruments, specially the wide spectral range achieved by combining them. We also encourage coordinated operations with other Mars-observing missions capable of supplying simultaneous measurements of its upper atmosphere